• Journal of the Korean Society of Environmental Restoration Technology
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• v.14 no.3
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• pp.157-168
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• 2011

In order to promote efficiency of upstream and downstream migration of fishes, this study has developed a combined fishway of herring-bone bottom baffle type and brush type fishways. The results obtained are as follows : 1. In a channel with constant incline, the velocity of current generally shows a distinct tendency of acceleration as it goes down the stream. But in the hydraulic experiment of herring-bone bottom baffle type fishway, the velocity reached its maximum only at 0.4m/sec, and it tended to be stable without any acceleration. 2. The velocity in the brush type fishway showed a distinct tendency of acceleration as the discharge increased. But its greatest velocity was only 0.3m/sec, and its velocity change according to the discharge increase was only 0.15m/sec at maximum. 3. The maximum velocity in the combined type fishway was less than half of the blast speed of the poorest swimmer, the juvenile eel with 90mm of body length. So any species of fishes are supposed to be able to migrate upstream from the estuary through this combined type fishway. 4. The field experiment of upstream migration showed that the combined type fishway can promote efficiency of upstream and downstream migration of any species of fishes.

• Korean Journal of Environment and Ecology
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• v.31 no.1
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• pp.54-61
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• 2017

We investigate predation rates of larvae and juveniles of Rana catesbeiana by using six native predatory fishes and six birds to select effective species to ecological control and management of R. catesbeiana that is invasive alien species and affects seriously wetland in Korea. Among freshwater fishes, Pseudobagrus fulvidraco, Silurus asotus and Channa argus ate larvae and juveniles of R. catesbeiana, and prefer the former, however Opsariichthys uncirostris, Monopterus albus and Anguilla japonica didn't eat at all. Six experimental water birds, Cygnus columbianus, Cygnus cygnus, Anas crecca, Aix galericulata, Anas penelope and Anas formosa nerver ate them. We were able to confirm control of R. catesbeiana probability using the native predatory fishes in Korea wetland.

• Development and Reproduction
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• v.23 no.2
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• pp.139-148
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• 2019

Rising of water temperature due to global warming is a great concern to aquaculturists and fishery biologists. Hence, the present study aimed to investigate the effects of high water temperature on juvenile red spotted grouper, Epinephelus akaara based on the evaluation of stress responses in blood. E. akaara juveniles were exposed to different thermal conditions ($25^{\circ}C$, $28^{\circ}C$, $31^{\circ}C$, and $34^{\circ}C$) for 6 weeks following 2 weeks of acclimation at $25^{\circ}C$. Blood cell morphology and number were examined at three sampling points (2, 7, and 42 days) from a total of 180 fish. Major erythrocytic cellular abnormalities (ECA) observed in blood smears of thermally stressed groups ($31^{\circ}C$ and $34^{\circ}C$) after 6 weeks were echinocytes, teardrop-like cells, swollen cells and vacuolated cells. Both red and white blood cell number (RBC and WBC) were significantly (p<0.05) elevated in $31^{\circ}C$ and $34^{\circ}C$ group after 6 weeks thermal exposure. Differential leucocytes number showed significant increases in neutrophil (N) and decreases in lymphocytes (L) in the highest temperature ($34^{\circ}C$). Different N:L ratio was observed at different thermal conditions which can be used as a reliable alternative to measure stress response. Taken together, these results suggest that higher temperature ($31^{\circ}C$ and $34^{\circ}C$) can interfere the immune system of red spotted grouper by altering the blood cell morphology and number.

• Korean Journal of Ichthyology
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• v.35 no.3
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• pp.151-159
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• 2023

This study investigated the early life history of the Micropercops swinhonis in Botongcheon stream Hangang River and compared the relationship between the related species and flexibility. The fertilized egg of the ovoid type, and the average egg diameter was 1.23 mm in long diameter and 0.85 mm in short diameter. At a water temperature of 20.6℃, the hatching time took 179 hours. The newly hatched larvae measured 3.82~4.08 mm in total length (TL), and their mouth and anus were not yet open. At 18 days after hatching, the postflexion larvae reached 7.97~8.43 mm in TL, with the tip of the caudal fin bent at 45°. At 29 days after hatching, the juveniles reached 9.83~10.8 mm in TL and were differentiated by their constant number of fins (7~10 first dorsal, 10~11 second dorsal, 7~10 anal fins). As a result of the study, there was a difference from the related species in the egg diameter, the newly hatched larva size. The postflexion larvae period and the development period of the first dorsal fin were the slowest among the comparable simila species, and the size was characterized by a large number, indicating a difference from similar species in the early life history process.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.26 no.5
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• pp.465-476
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• 1993

Rockfish, Sebastes inermis is a ovoviviparous fish distributed around the coastal waters of Korea and Japan. Naturally beared larvae obtained from female fish kept in the aquarium were reared for three months in the laboratory. The naturally healed larvae were 6.05mm in average total length(TL) with $8+18{\sim}19=26{\sim}27$ myomeres. Melanophores were distributed on the top of head, around the intestine tube and on the dorsal and ventral margins of the tail, but none on the pectoral fins. The larva(7.1mm in TL) consumed all the yolk and oil globule, in 7 days after bearing, and jaw bones were more rapidly ossified than vertebrae and cranium. In 21 days after bearing, the larvae averaged 8.50mm in TL and the caudal notochord flex $45^{\circ}$. Fin development in S. inermis larvae proceeds in the following sequence: caudal, pectoral, dorsal, anal and ventral fin. Individuals $17.6{\sim}20.8mm$ in TL($65{\sim}69$ days after bearing) are regarded as the juvenile stage. All fins bones was nearly completed in this stage.

• Journal of fish pathology
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• v.17 no.3
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• pp.159-169
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• 2004

Bacterial wihte enteritis ocurred by infection of V. ichthyoenteri is a devastating disease in olive flounder (Paralichthys olivaceus) hatcheries in Korea. Since white enteritis has been a problem in aquqtic industries, necessity of a rapid detection method is increased. In an attempt to develop rapid PCR method the detection of V. ichthyoenteri, we examined the 16S-23S rRNA intergenic spacer region(ISR) of V. ichthyoenteri and developed species-specific primer for V. ichthyoenteri. The intergenic spacers were amplified by primers complementary to conserved region of 16S and 23S rRNA genes. The intergenic spacer region between the 16S and 23S rRNA genes of V. ichthoenteri were investigated by PCR fragment length typing and DNA sequencing. Analysis of the ISR sequences showed that V. ichthyoenteri contains one types of polymorphic ISRs. The size of ISRs ranged 348bp length and not contains tRNA genes. Mutiple alignment of representative sequences from different Vibrio species revealed several domains of high sequence variability, and allowed to design species-specific primer for detection of Vibrio ichthyoenteri. PCR. The specific of the primer was examined using genomic DNA prepared from 19 different Vibrio species, isolated 18group Vibrio species. The results showed that the PCR reaction using species-specific primer designed in this study can be used to detect V. ichthyoenteri.

• Journal of Aquaculture
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• v.13 no.3
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• pp.223-230
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• 2000

A rearing experiment of the olive flounder was performed in a set of water-reuse system to test the reusability of the water in culture system with (i) a foam fractionator to separate particles from water and (ii) a culture tank contain mineral particles to filter the metabolic wastes by adsorption and/or decomposition. Two kinds of commercially processed loess particles and a dolomite particle (all 50 ${\mu}$diameter) were tested. The mineral particles were suspended in the culture tank and the water was pumped into the foam fractionator, where the particles were separated and drained out with foam from the system. In a circular culture tank of 4.8 m in diameter with 10 d water, the juvenile olive flounders (23.1 g/fish, 5,555 fish, 128 kg total body weight) were stocked. 90 % of the rearing water was reused and turnover rate of the water in the tank was two times per hour. Water temperature was maintained 17${\pm}$1$^{\circ}C$. At the end of 75 day-experimental rearing, 5,532 flounders, weighing 468 kg, were harvested. An individual flounder grew to 84.6 g of body weight. The final stocking density was 26.0 kg/$m^2$. No diseases were observed during the experiment.

• Korean Journal of Ichthyology
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• v.23 no.3
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• pp.198-205
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• 2011

Early life history of the endangered species, Gobiobotia macrocephala was investigated to provide baseline data for biological characteristics and recovery. Adult fish were sampled by spoon net at Hongcheon-gun, Gangwon-do, Korea in April to June 2010. The female's eggs were obtained by injecting Ovaprim and fertilized by dry method in the laboratory. The mature eggs were slightly adhesive and transparent with greyish and average 0.89${\pm}$0.04mm in diameter. The hatching of the embryo took place in about 107 hours after fertilization under water temperature of 23$^{\circ}C$ and newly hatched larvae were average 4.6${\pm}$0.16mm in total length. At 4 days after hatching, they were average 6.1${\pm}$0.20mm in total length and their yolk sacs were completely absorbed. From 15 days after hatching, they entered the juvenile stage and reached at 8.6${\pm}$0.67mm in total length. At the 100 days after hatching, their band patterns and external form were similar to those of adults, and they averaged 31.5${\pm}$3.32mm in total length.

• Korean Journal of Ichthyology
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• v.29 no.1
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• pp.52-61
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• 2017

The egg development and early life history of Korean endemic fish, Squalidus multimaculatus (Gobioninae), were investigated. The eggs from the females were obtained by injecting 10 IU/g of human chorionic gonadotropin and inseminated by wet method in the laboratory. The fertilized eggs were 0.8~0.9 mm in diameter and had no oil globules. The embryo began to hatch about 65 hrs after fertilization under water temperature of $24{\pm}1^{\circ}C$. The newly-hatched larvae were 2.5~3.1 mm in total length, and their mouth and anus were not opened. Four days after hatching, the postlarva were 4.0~4.2 mm in total length, and their york sacs were completely absorbed. They entered the juvenile stage when all fin-rays were formed at 30 days after hatching, and their total length were 11.2~15.7 mm. At 45 days after hatching, the external from of juveniles were similar to those of adults (total length were 18.8~22.5 mm), and 80 days after hatching, the external characteristics from of juveniles were same to adults (total length were 25.7~35.9 mm).

• Journal of fish pathology
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• v.19 no.2
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• pp.87-97
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• 2006

In previous study, scuticociliate P. dicentrachi was isolated from farmed flounder during the december 2004 to april 2005. Pathogenicity, mortality and infection symptom were studied using 3 cm and 5 cm groups of juvenile flounder. The ciliates were exponentially increased from bottom layer of the experimental tanks, which propagated within 2000 cells/ml (1.4 × 103 cell ㎖-1 ～ 2.5 × 103 cell ㎖-1) after 6 days of inoculation. The middle layer was maintained within 300 cells/ml. Both 3 cm and 5 cm groups were infected with ciliates, mainly 3 cm group showed high mortality during the experimental period. The death of 3 cm group was started from 5 days and showed 95.6% of mortality after 28 days of first inoculation. The control group showed 4.4% of mortality however, we could not observed any ciliates. The death of 5 cm group was started later than 3 cm group after 18 days of first inoculation. The total mortality was 71% during 28 days. No mortality and infection symptoms were observed in the control. We also studied SSU rRNA gene of ciliates which, re-isolated from infected flounder of experimental groups. When SSU rRNA in this study compared with previous data showed that the identified strain of both previous and present study was same.