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Changes of the Structural and Biomechanical Properties of the Bovine Pericardium after the Removal of ${\alpha}$-Gal Epitopes by Decellularization and ${\alpha}$-Galactosidase Treatment

  • Nam, Jinhae;Choi, Sun-Young;Sung, Si-Chan;Lim, Hong-Gook;Park, Seong-Sik;Kim, Soo-Hwan;Kim, Yong Jin
    • Journal of Chest Surgery
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    • v.45 no.6
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    • pp.380-389
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    • 2012
  • Background: Bovine pericardium is one of the most widely used materials in bioprosthetic heart valves. Immunologic responses have been implicated as potential causes of limited durability of xenogenic valves. This study aimed to determine the effectiveness of decellularization and ${\alpha}$-galactosidase (${\alpha}$-gal) to remove major xenoreactive antigens from xenogenic tissues. Materials and Methods: Recombinant Bacteroides thetaiotaomicron (B. thetaiotaomicron) ${\alpha}$-gal or decellularization, or both were used to remove ${\alpha}$-gal from bovine pericardium. It was confirmed by ${\alpha}$-gal-bovine serum albumin-based enzyme-linked immunosorbent assay (ELISA), high-performance anion exchange chromatography, flow cytometry, 3,3'-diaminobenzidine-staining, and lectin-based ELISA. The mechanical properties of bovine pericardium after decellularization or ${\alpha}$-gal treatment were investigated by tests of tensile-strength, permeability, and compliance. Collagen fiber rearrangement was also evaluated by a 20,000${\times}$ transmission electron microscope (TEM). Results: Recombinant B. thetaiotaomicron ${\alpha}$-gal could effectively remove ${\alpha}$-gal from bovine pericardium B. thetaiotaomicron (0.1 U/mL, pH 7.2) while recombinant human ${\alpha}$-gal removed it recombinant human ${\alpha}$-gal (10 U/mL, pH 5.0). There was no difference in the mechanical properties of fresh and recombinant ${\alpha}$-gal-treated bovine pericardium. Furthermore, the TEM findings demonstrated that recombinant ${\alpha}$-gal made no difference in the arrangement of collagen fiber bundles with decellularization. Conclusion: Recombinant B. thetaiotaomicron ${\alpha}$-gal effectively removed ${\alpha}$-gal from bovine pericardium with a small amount under physiological conditions compared to human recombinant ${\alpha}$-gal, which may alleviate the harmful xenoreactive immunologic responses of ${\alpha}$-gal. Recombinant ${\alpha}$-gal treatment had no adverse effects on the mechanical properties of bovine pericardium.

Estimation of Eutrophication during Summer and Fall in Danghang Bay (당항만의 여름과 가을의 부영양화 평가)

  • Kim, Sung Jae;Yoo, Young Jin
    • Journal of Wetlands Research
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    • v.19 no.4
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    • pp.383-392
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    • 2017
  • In 2013, August and September(early) as summer and October and November as Fall the probe of eutrophication has been done at 22 sampling points from the entrance of Danghang Bay (Jinhae Bay) to Geosan reservoir. In Danghang Bay, total chlorophyll(TChl) concentration of summer was 3.7 times higher than that of fall, and sampling points closer to the center showed 1.8 times higher concentrations than sampling points near the fringe where fresh water encountered. Eutrophication Index(EI) exceeded 1 at all sampling points in Danghang Bay during summer and fall, and if other conditions for algae growth met there was a possibility red tide to bloom at any place. There was a tendency of EI to gradually increase moving up from the entrance of bay to the inner side during summer and fall. Especially there was a sudden increase by 2.3 times higher at sampling points of 13~22 (planned region as Madong reservoir) than at other points during fall. Nitrogen was a limiting nutrient for growth of algae during summer and fall in Danghang Bay, but phosphorus was a limiting nutrient during summer rainy season. During summer and fall, multiple linear regression analysis between EI and COD, DIN, and DIP showed a significant positive relationship and that DIP was the most effective variable. Whereas multiple linear regression analysis between TChl and COD, DIN, DIP, and DSi showed a significant positive relationship and that DIP was also the most effective variable during summer. There was no significant correlation between TChl and the other parameters during fall.

Occurrence and Survival Rate of the Larvae of Sea Mussel, Mytilus edulis (담치 종묘생산기술개발에 관한 연구 -진주담치 Mytilus edulis 부유유생의 출현과 생존율)

  • YOO Sung Kyoo;KANG Kyoung Ho;LEE Dong Yeub
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.21 no.1
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    • pp.35-41
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    • 1988
  • In order to set up a predictive model for effective spat collection of sea mussel Mytilus edulis , the survival rate and time required at each developmental stage of drifting larvae were surveyed during the period from March 14 to July 20 in 1987 at the Naesan Ri, Jinhae Bay, the southern part of Korea. The advent of D - shape larvae ca. $120\times90um$ um long had three peaks in that area: April 15, May 13 and June 7. Umbo shape larvae ca.$188\times162{\mu}m$ and full grown larvae ca. $289\times280{\mu}m$ long also showed three peaks: April 27. May 24 and June 20 for the former, and May 10, June 5 and June 30 for the latter. Eleven to thirteen days were required for D - shape larvae to develop to umbo - shape larvae. The instantaneous death rate was 0.1300 and the daily survival rate 0.8781 at this intermorphological stage. The turnover time of umbo to full grown larvae varied from ten to thirteen days with a instantaneous death rate of 0.1520, daily survival rate of 0.8590, and mean survival rate of $16.89\%$. Twenty - three to twenty - five days were required for each group of the D - shape larvae to reach a full grown stage, and their mean survival rate was $3.55\%$ during this developmental period.

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Estimation of the Range of the Suspended Solid from the Nakdong River using Satellite Imageries and Numerical Model (위성영상 및 수치모델을 이용한 낙동강유출 부유토사 확산범위 추정)

  • Hwang, Jae-Dong;Kang, Yong Q.;Suh, Yong-Sang;Cho, Kyu Dae;Park, Sung Eun;Jang, Lee-Hyun;Lee, Na Kyung
    • Journal of the Korean Association of Geographic Information Studies
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    • v.5 no.2
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    • pp.25-33
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    • 2002
  • We were trying to understand indirectly the range of the discharge from the Nakdong with the dispersion of suspended solid(SS) related to the amount of discharge from river in this study. The range of dispersion of SS from the Nakdong was estimated using satellite remote sensing and numerical modeling. The stream field with two dimensional and numerical model using the condition of integrated depth was calculated. According to the results, the streamline flowed from Busan to the Jinhae Bay and Geojae Island. at the flood. The situation at the ebb was totally changed. The streamline flowed out Busan from the Bay. The velocity in offshore was faster than one at coastal water of the Nackdong. Residual current which was averaged during 12hours dominantly appeared the dominant direction from the southwestern part of the Nackdong to the northeastern part of it. The eastward current appeared at the eastern coast of Gaduck Is. Base on the results of the velocity field, the quantifying of the dispersion of SS was estimated by the method of numerical tracer related to the Lagrangian method. The significant range of the dispersion of the SS from the Nackdong was from the eastern coast of Gaduck Is, to the coastal areas of Busan, Korea. The estimated range of the dispersion of the SS using the SeaWiFS and Landsat satellite data was similar to the estimated results using the numerical model.

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Quality Characteristics of Yogurt Prepared with Flowering Cherry (Prunus serrulata L. var. spontanea Max. wils.) Fruit Powder during Storage (버찌(Fruit of Prunus serrulata L. var. spontanea Max. wils.) 분말을 첨가한 요구르트의 저장 중 품질 특성)

  • Kim, Kyoung-Hee;Hwang, Hye-Rim;Jo, Ji-Eun;Lee, Sang-Young;Kim, Na-Young;Yook, Hong-Sun
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.38 no.9
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    • pp.1229-1236
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    • 2009
  • This study was performed to examine the quality characteristics of the curd yogurt with different contents (0.5$\sim$2.0% (w/v)) of cherry powder (obtained from freeze-dried fruit of Prunus serrulata L. var. spontanea Max. wils.). Yogurt was fermented with two kinds of lactic acid bacteria (Streptococcus thermophilus, Lactobacillus bulgalicus) at $40^{\circ}C$ for 18h. During the period of 14 days of storage ($4^{\circ}C$, 14 days), acid production (pH and titratable acidity) of the yogurts increased significantly with the addition of cherry powder. The sugar content of the yogurts prepared with cherry powder increased but cherry powder yogurts presented lower viscosity values than the control. Hunter color values, lightness (L) and yellowness (b) of yogurt colors decreased as the concentration of cherry powder increased, whereas the redness (a) increased. Lactic acid bacteria of yogurts increased with the addition of cherry powder and all samples had increasing levels of lactic bacteria over the storage period. The sensory properties, such as flavor, taste, viscosity, and overall acceptability, of the yogurts containing the 1.0% cherry powder were superior to the control sample. Consequently, the optimal quality achieved in these experiments was yogurt with 1.0% cherry powder added.

Possibility of Control of Turfgrass Insect Pest, Popillia quadriguttata(Coleoptera : Rutelidae) Using Pheromone Trap in Golf Course (페로몬 트랩을 이용한 골프장 잔디해충 녹색콩풍뎅이의 방제가능성)

  • Kim, Jae-Ho;Lee, Jae-Pil;Ham, Suon-Kyu;Kim, Doo-Hwan;Yeom, Ju-Rip;Lee, Dong-Woon
    • Asian Journal of Turfgrass Science
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    • v.23 no.1
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    • pp.45-60
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    • 2009
  • Occurrence ecology and possibility of mass trapping of Popillia quadriguttata, one of the most serious turfgrass insect pest in domestic golf courses was investigated using pheromone traps in Gimpo and Jinhae. Peak period of adult occurrence of P. quadriguttata was late June and early July in Gimpo Seaside Country Club from 2006 to 2008. It was early and middle July in Youngwon Country Club in 2006. The first catched day of P. quadriguttata adult was 20 June, while the last day was 10 September in Gimpo Seaside Country Club, 2006. Mean catched number of P. quadriguttata adult in Japanese beetle pheromone trap per day in peak period of occurrence were 18.2, 25.7, 29.0, and 15.7 at 2005, 2006, 2007 and 2008 respectively. Mean catched number of P. quadriguttata adult in Japanese beetle pheromone trap were more in fairways or roughs than tees or greens. Possibility of control of scarab beetle using pheromone trap with pesticide treatment was investigated against oriental beetle, Blitopertha orientalis in Dongrae Benest Golf Club. Mean number of dead oriental beetle adult were more higher(2.9 folds) in fenitrothion EC treatment than fenitrothion EC treatment after set oriental beetle pheromone trap.

The Assessment of Trophic State and the Importance of Benthic Boundary Layer in the Southern Coast of Korea (한국남부 연안의 영양상태 평가와 저층 경계면의 중요성)

  • 이재성;김기현;김성수;정래홍;박종수;최우정;김귀영;이필용;이영식
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.9 no.4
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    • pp.179-195
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    • 2004
  • The trophic state of the coastal waters of the southern part of Korea was assessed using biogeochemical data obtained from the National Marine Environmental Monitoring Program conducted by the National Fisheries Research and Development Institute for six years. The trophic state of different areas, analyzed by non-metric multi-dimensional scaling (MDS) analysis, could divide the areas into three groups. Masan Bay, with suboxic water masses and/or the highest concentrations of dissolved inorganic nitrogen and phosphorus occurred, was assessed as being in a hypertrophic state. Ulsan Bay, Onsan Bay, Busan and Jinhae Bay, located near strong point sources, were in a eutrophic state. Other areas, including Tongyong, Yosu, Mokpo and Jeju island, were evaluated as being in a mesotrophic state. During 1997 to 2002, the average values of excess nitrogen, which is the difference between the measured dissolved inorganic nitrogen (DIN) and the corrected DIN using the Redfield ratio, were positive at Ulsan, Onsan, and Busan, where there were inflows from polluted rivers. In contrast, those were negative values in Haengam Bay, Gwangyang Bay and nearby Yosu. This suggests that the limiting element for phytoplankton growth differed among sites. The time series data of excess nitrogen showed gradual decrease over time in the hypertrophic waters, but the opposite trend in the mesotrophic waters. This indicated that the ratio of nitrogen to phosphate varied according to the trophic state of the coastal waters. The enrichment of organic matter in sediment in eutrophic waters would disturb the normal pattern of biogeochemical cycling of nitrogen and phosphate. In order to assess the condition of the coastal environment, the benthic boundary layer should be considered.

Evaluation of Offshore Water Quality by Bioassay Using the Gametes and Embryos of Sea Urchins (성게 알을 이용한 생물검정에 의한 연안해수 수질평가에 관한 연구)

  • Yu, Chun-Man
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.3 no.3
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    • pp.170-174
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    • 1998
  • The water quality of offshore waters around the Korean coast was evaluated by bioassay using gametes, embryoss and early development systems of a sea urchin species Hemicentrotus pulcherrimus. The results show that despite the inflows of several river systems, the west coast maintain the grade II water. This is thought to be due to the decrease in pollutant input resulting from the purification of inflow rivers (e.g., the control of waste water discharge and the construction of sewage treatment facilities), and the dilution of pollutants by a strong tidal mixing with a large difference between the ebb and flood tides. However, Asan, Hampyong and Chonsu bays, where circulations of water are relatively poor, and the Kunsan and Mokpo harbors which are influenced by pollutants from neighboring cities have shown the grade III water. The south coast has maintain the grade II or III because developments of sea urchins were moderately or strongly inhibited. Also, Kangjin, Duekryang, Kwangyang, Masan, and Jinhae bays where water circulations are relatively poor, show the grade III water, with strong inhibitions of the early development of sea urchins. The east coast has maintained the grade I and II due to monotonous coastlines and smooth circulation of sea water. However, Chongcho Lake shows the worst water quality due to the breakwater which is constructed to maintain port functions.

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Efficient eliminating methods of noises imbedded acoustic signals (음향 신호에 내재된 잡음의 효율적인 소거 방법)

  • PARK, Junseong;LEE, Jeong-Hoon;KIM, Doo Nam;PARK, Yeongguel;JANG, Choong-Sik;PARK, Byung-Soo;KANG, Myounghee
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.51 no.3
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    • pp.432-440
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    • 2015
  • The hydro-acoustic technology has been widely used in not only South Korea but also many foreign countries for various scientific purposes. Unfortunately acoustic data especially collected from field surveys may contain noises caused by a variety of sources. Therefore, it is exceedingly important to eliminate noises when acoustic data are analyzed to derive quantitative results. This study introduced two methods for eliminating noises easily and effectively using post-processing software. Used acoustic data were collected on the Jinhae bay and Tongyeong coast of the South Sea in April 2015. The first method, that is the Wang's method, placed emphasis on 'erosion filter' to eliminate only data samples contaminated by noises. The second method (Yamandu's method) focused on the 'resample by number of pings' to remove pings contained noises. To substantiate the effectiveness of two methods, the mean Sv (Volume backscattering strength), mean height and depth of the fish schools detected were compared between before and after using the noise elimination methods. In the Wang's method the mean Sv was decreased from -52.4 dB to -52.9 dB, and in the Yamandu's method from -52.6 dB to -53.3 dB, indicating that noises were successfully eliminated. The mean height (1.5 m) and depth (19.0 m) were same between before and after using two methods showing that the shapes of fish schools were not changed.

Elderly Suicide and It's Related Factors : Focused on the Role of Social Support and Mastery in the Effects of Hopelessness and Depression on Suicidal Ideation (노인 자살관련 요인: 무망감과 우울증이 자살생각에 미치는 영향에 대한 사회적 지지와 자아통제감의 효과를 중심으로)

  • Eom, Tae-Wan
    • Korean Journal of Social Welfare
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    • v.59 no.2
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    • pp.355-379
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    • 2007
  • This study examined the role of social support and mastery in the effects of hopelessness and depression on suicidal ideation in order to identify elders' suicide and it's related factors. Especially, it focused to find out whether the hopelessness excluding depression has an independent effect on elders' suicidal ideation, and psychosocial factors such as social support and mastery has an interaction effects with elders' hopelessness variables and depression on suicidal ideation. This study recruited 297 elders over 65(older persons in the elder education programs of senior welfare center in Masan, Changwon and Jinhae) and employed a self-administered survey method during January, 2007. The following are the major results of the study. First, the level of hopelessness, depression and suicidal ideation related elders' suicide indicated differences according to their educational level, marital state, income, and their chronical disease numbers. Especially, the lower income and higher chronical disease numbers showed higher level of hopelessness, depression and suicidal ideation among the elder subjects. Second, interaction effects of social support and depression were significant in suicidal ideation, the more level of social support in elder' subjects, the lower the suicidal ideation. While, interaction effects of social support and hopelessness, and mastery and depression/hopelessness in suicidal ideation were not statistically significant. Third, psychosocial factors such as social support and mastery on elders' suicidal ideation have a significant effect when other relevant variables are controlled. From these results, hopelessness is other significant factor predicting suicidal ideation(suicide) among elders. Also, social support and mastery is very significant factor influencing to prevent or decrease suicidal ideation among elders.

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