Micro-scale serpentine structure fibers are widely used as flexible sensor in the manufacturing of micro-nano flexible electronic devices because of their outstanding non-linear mechanical properties and organizational flexibility. The use of melt electrowriting (MEW) technology, combined with the axial bending effect of the Taylor cone jet in the process, can achieve the micro-scale serpentine structure fibers. Due to the interference of the process parameters, it is still challenging to achieve the precise deposition of micro-scale and high-consistency serpentine structure fibers. In this paper, the micro-scale serpentine structure fiber is produced by MEW combined with axial bending effect. Based on the controlled deposition of MEW, applied voltage, collector speed, nozzle height and nozzle diameter are adjusted to achieve the precise deposition of micro-scale serpentine structure fibers with different morphologies in a single motion dimension. Finally, the influence mechanism of the above four parameters on the precise deposition of micro-scale serpentine fibers is explored.
Jinhuo Zheng;Minglong Shen;Shifang Tu;Zhibo Chen;Xiaodong Ni
Geomechanics and Engineering
/
v.36
no.6
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pp.563-573
/
2024
In this study, various countermeasures used to mitigate tunnel deformations due to nearby multi-propped basement excavation in soft clay are explored by three-dimensional numerical analyses. Field measurements are used to calibrate the numerical model and model parameters. Since concrete slabs can constrain soil and retaining wall movements, tunnel movements reach the maximum value when soils are excavated to the formation level of basement. Deformation shapes of an existing tunnel due to adjacent basement excavation are greatly affected by relative position between tunnel and basement. When the tunnel is located above or far below the formation level of basement, it elongates downward-toward or upward-toward the basement, respectively. It is found that tunnel movements concentrate in a triangular zone with a width of 2 He (i.e., final excavation depth) and a depth of 1 D (i.e., tunnel diameter) above or 1 D below the formation level of basement. By increasing retaining wall thickness from 0.4 m to 0.9 m, tunnel movements decrease by up to 56.7%. Moreover, tunnel movements are reduced by up to 80.7% and 61.3%, respectively, when the entire depth and width of soil within basement are reinforced. Installation of isolation wall can greatly reduce tunnel movements due to adjacent basement excavation, especially for tunnel with a shallow burial depth. The effectiveness of isolation wall to reduce tunnel movement is negligible unless the wall reaches the level of tunnel invert.
Kim, Hyun Jung;Lee, Cho Ae;Hwang, Eun Kyung;Han, Man Young;Ann, Uk Sung;Cho, Young Min
Clinical and Experimental Pediatrics
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v.46
no.12
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pp.1235-1241
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2003
Purpose : We assessed the dynamic characteristics of 16 nebulizer/compressor combinations currently available in Korea. Methods : The 16 nebulizer/compressor combinations(Pariboy Type 38/Long life, Pariboy Type N/Long life, Pariboy Type N/Salter 8900, Pariboy Type N/LC, Devilbiss pulmoaid-LT/Hudson, Devilbiss pulmoaid/Hudson, Mesmed neb-300/Own, San-up 3040/Hudson, Midas(Basic)/Own, AirJolie 2/Hudson, Thomas 1127/Salter 8900, Noel NE-2000/Salter 8900, Omron CX3/Hudson, Chang Woo CWN-100/Salter 8900, Voyage/Mefar, Chang Woo ASI-Pro/Medel jet pulse) were evaluated in terms of particle size and mass output. In addition, we determined the effects of nebulizer fill volume on mass output. Results : Pariboy Type N/Long life has the highest respirable mass of 0.184 mg/min and Mesmed Neb-300/Own has the lowest 0.019 mg/min. Pariboy Type N/Long life has the highest mass output of 0.68 mg/min and the shortest mass median aerodynamic diameter(MMAD) of $3.76{\mu}m$. All combinations other than Pariboy Type N/Long life produced a MMAD of over $5{\mu}m$. MMAD over a 5 min nebulization ranged 3.76 to $9.83{\mu}m$. There were no significant effects of fill volume on mass output. Conclusion : We concluded that there is a wide variation in performance of nebulizer/compressor combinations. The characteristics of nebulizer/compressor combinations should be considered in selecting products.
Lee, Yong-Boum;Lee, Doo-Soo;Cho, Sang-Cheol;Shin, Sang Tae
Journal of Embryo Transfer
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v.29
no.1
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pp.35-41
/
2014
Laparoscopic ovum pick-up (LOPU) is a convenient method for collecting oocytes in small ruminants. LOPU has the advantage of being a less invasive means of oocyte collection, thereby allowing for a repeated usage of the oocyte donor animals. A total of 25 Korean black goats were used in the winter season (December to February) and LOPU was applied to the goats which had been treated for superovulation more than two times during the last twelve months. Estrus was synchronized with an intravaginal insert containing 0.3 g progesterone for 10 to 12 days. Ovaries were hyperstimulated with eCG 1,000 IU oneshot, FSH with eCG (50 mg / 1,000 IU; 70 mg / 500 IU; 70 mg / 1,000 IU) oneshot or FSH multiple-shot with eCG oneshot ($20mg{\times}6/300IU$) given intramuscularly 72 h prior to LOPU. For these groups, the number of follicles (mean ${\pm}$ SEM) observed which developed to larger than 2 mm in diameter were $1.6{\pm}2.5$, $4.3{\pm}3.1$, $5.5{\pm}4.2$, $6.6{\pm}2.1$ and $8.8{\pm}7.8$, respectively. Oocytes were aspirated by using OPU needles and a vacuum pump. The overall oocyte retrieval rates were 41.4%. Oocytes were matured in TCM-199 supplemented with 10% (w/v) bovine serum albumin + $10{\mu}g/ml$ FSH + $1{\mu}g/ml$$17{\beta}$-estradiol for 27 h at $39^{\circ}C$ in 5% $CO_2$ in air. Oocytes were parthenogenetically activated by ionomycin combined with 6-diethylaminopurine (6-DMAP). Total oocyte maturation and cleavage rate were 67.3% and 78.8%, respectively. In summary, LOPU is a useful oocyte collection method in Korean black goats that can provide immature oocytes for transgenesis or nuclear transfer.
In this study, scenarios based on the leakage of highly compressed air and fire occurrence turned out to be high risks in an operation stage of CAES facility were constructed and estimated. By combining Bernoulli equation with momentum equation, an expression to calculate an impact force of a jet flow of compressed air was derived. An impact force was found to be proportional to the square of diameter of fracture and the pressure of compressed air. Four types of fire scenarios were composed to evaluate an effects that seasonal change and location of fire source have on the spread behavior of smoke. Smoke from the fire ignited in the vicinity of CAES opening descended more quickly below the limit line of breathing than one from the fire occurred 10 m away from CAES opening, which is expected to occur due to a propagation of wave front of smoke. It was shown that a rate of smoke spread of the winter fire is faster than one of the summer fire and smoke from the winter fire spreads farther than one of the summer fire, which are dependent on the direction of air flow into access opening. Evacuation simulation indicated that the required safe evacuation time(RSET) of the summer and winter fires are 262, 670 s each.
This study was canted out to develop cell lines overexpressing human H-transferase (HT). One of the approaches to prevent hyperacute rejection in xenotransplantation might be the expression of human HT in porcine cells. In this study, we cloned human HT gene from HepG2 cells using RT-PCR to establish HT-overexpressing vector. The full-length cDNA of human HT was inserted into the 3' end of CMV promoter for construction of the overexpression vector pRc/CMV-hHT. Using ietPEI DNA transfection reagent, the vector was introduced into porcine ear skin fibroblasts from newborn piglets. Transfected cells were selected by treatment of $300{\mu}g/ml$ G418 for 12 days. After antibiotic selection, survived colonies with approximately 5mm in diameter were picked and analysed for transgene human HT by PCR. The colonies proven to be human HT transfectants were analysed by RT-PCR to determine their expressions or human HT. In all colonies tested, human HT mRNA was detected. This result demonstrates the establishment of porcine cell lines overexpressing human HT, and these cell lines may be used for the development of transgenic pigs for xenotransplantation.
For a methanol separation column of the BPA (Bisphenol A) plant, HAZOP (hazard and operability) assessment was performed and damage ranges were predicted from the accident scenarios for the fire and the explosion. As a result, the damage range of the jet fire was 20 m in the case of rupture of the discharge pipe (50 mm diameter) of safety valve, and that of the flash fire was 267 m in the case of catastrophic rupture. Also, the damage ranges of the unconfined vapor cloud explosion (UVCE) for the rupture of the discharge pipe and for the catastrophic rupture were 22 m and 542 m, respectively. For the worst case of release scenarios, safety measures were suggested as follows: the pressure instruments, which can detect abnormal rise of the internal pressure in the methanol separation column, should be installed by the 2 out of 3 voting method in the top section of the column. Through the detection, the instruments should simultaneously shut down the control and the emergency shut-off valves.
Zona pellucida (ZP), a primarily representative coat of mammalian egg and embryo, has an extremely heterogeneous morphology during different developmental stages. The objective of the present study was to compare the morphological changes of the ZP surface of immature, in vitro and in vivo matured canine oocytes by using scanning electron microscopy (SEM). Canine ovaries were collected from local veterinary hospitals to recover immature oocytes. The ovaries were sliced and the released cumulus oocyte complexes (COCs) were washed with TL-HEPES. The selected COCs were randomly divided into two groups, first group was processed immediately at immature state and the second group was processed 72 h after in vitro maturation, and compared with in vivo derived oocytes. Oocytes were fixed, critical point dried and examined under SEM. The diameters of oocyte and outer holes of the ZP were measured on a total of 249 oocytes; the results were analyzed using One-way ANOVA. Our results showed that, the diameter of immature oocytes significantly differed (p < 0.05) from that of in vivo matured oocytes ($79.60{\pm}0.77{\mu}m$ vs. $101.46{\pm}1.07{\mu}m$, respectively). Similarly, a significant difference (p < 0.05) in the diameters between those of in vitro and in vivo matured oocytes were found ($79.51{\pm}2.36{\mu}m$ vs. $101.46{\pm}1.07{\mu}m$, respectively). Moreover, the diameters of the outer holes of the ZP were significantly (p < 0.05) larger in in vivo matured ($1.48{\pm}0.42{\mu}m$) than in vitro matured for 72 and immature oocytes ($1.10{\pm}0.16$ and $0.43{\pm}0.12{\mu}m$, respectively). Taken together, these data indicates that the ZP surface is related to oocyte maturity in canine.
Veneers of two nominal thicknesses, 1.5 and 2.5mm, were rotary peeled from Japanese larch (Larix leptolepis). Dahurian larch (Larix gmelinei) and Radiata pine (Pinus radiata) bolts unheated and heated in the water vat temperature of $66^{\circ}C$ by rising a final temperature at 10cm core of $60^{\circ}C$ to remain core diameter of 11.4cm. 1.5 and 2.5mm thick veneer cut from the heated Japanese larch were significantly thinner than those of the unheated bolt and 2.5mm thick veneer cut from the heated Dahurian larch were significantly thicker than that of the unheated bolt. 1.5mm thick Dahurian larch veneer and 1.5 and 2.5mm thick Radiata pine veneers showed insignificant difference between the unheated and heated bolts, respectively. Check distance on the loose side of 1.5mm thick veneer cut from the heated Radiata pine was significantly wider than that of the unheated bolt. However check distances on the loose side of 1.5 and 2.5mm thick Japanese larch and Dahurian larch veneers and 2.5mm thick Radiata pine veneers showed insignificant difference between the unheated and heated bolts. Also the depth of check on the loose side of three species showed insignificantly difference between the unheated and heated bolts. Arithmatic mean deviation($R_a$) and maximum height($R_{max}$) of the profile on the loose side of dried veneer by preheating the bolt compared with unheated bolts were different among apecies and between veneer thickness. The preheating treatment slightly affected qualities of these thin veneers such as 1.5 and 2.5mm. The yield of 2.5mm thick veneer from the heated radiata pine was significantly higher than that of the unheated bolt. However the yield of 2.5mm thick veneer for other two species and 1.5mm thick veneer for three species showed insignificant difference between the unheated and heated bolts. The yield of 2.5mm thick veneer for three species were higher than those of 1.5mm thick veneer. The average yields of green veneer of Japanese larch. Dahurian larch and Radiata pine were 57.1, 55.1 and 54.0 percent, respectively. Variables such as initial MC. drying time and veneer thickness had strong effect for Japanese larch veneer, less effect for Radiata pine veneer and nuch less effect on final MC for Dahurian larch veneer in jet drying. Correlation between the current MC and the drying time of Dahurian larch with low variation of initial MC was higher than those of Japanese larch and Radiata pine veneer with high variation of initial MC in high temperature drying. Thickness shrinkages of 2.5mm thick veneer for Japanese larch and Radiata pine were higher than those of 1.5mm thick veneers, but shrinkages of Dahurian larch veneer were similar between two nominal veneer thicknesses.
Background: Minimal pressure support(PSmin) is a level of pressure support which offset the imposed work of breathing(WOBimp) developed by endotracheal tube and ventilator circuits in pressure support ventilation While the lower applied level of pressure support compared to PSmin could induce respiratory muscle fatigue, the higher level than PSmin could keep respiratory muscle rest resulting in prolongation of weaning period during weaning from mechanical ventilation PSmin has been usually applied in the level of 5~10 cm$H_2O$, but the accurate level of PSmin is difficult to be determinated in individual cases. PSmin is known to be calculated by using the equation of "PSmin = peak inspiratory flow rate during spontaneus ventilation$\times$total ventilatory system resistance", but correlation of calculated PSmin and measured PSmin has not been known. The objects of this study were firstly to assess whether customarily applied pressure support level of 5~10 cm$H_2O$ would be appropriate to offset the imposed work of breathing among the patients under weaning process, and secondly to estimate the correlation between the measured PSmin and calculated PSmin. Method : 1) Measurement of PSmin : Intratracheal pressure changes were measured through Hi-Lo jet tracheal tube (8mm in diameter, Mallinckroft, USA) by using pulmonary monitor(CP-100 pulmonary monitor, Bicore, USA), and then pressure support level of mechanical ventilator were increased until WOBimp was reached to 0.01 J/L or less. Measured PSmin was defined as the lowest pressure to make WOBimp 0.01 J/L or less. 2) Calculation of PSmin : Peak airway pressure(Ppeak), plateau airway pressure(Pplat) and mean inspiratory flow rate of the subjects were measured on volume control mode of mechanical ventilation after sedation. Spontaneous peak inspiratory flow rates were measured on CPAP mode(O cm$H_2O$). Thereafter PSmin was calculated by using the equation "PSmin = peak inspiratory flow rate$\times$R, R = (Ppeak-Pplat)/mean inspiratory flow rate during volume control mode on mechanical ventilation". Results: Sixteen patients who were considered as the candidate for weaning from mechanical ventilation were included in the study. Mean age was 64(${\pm}14$) years, and the mean of total ventilation times was 9(${\pm}4$) days. All patients except one were males. The measured PSmin of the subjects ranged 4.0~12.5cm$H_2O$ in 14 patients. The mean level of PSmin was 7.6(${\pm}2.5\;cmH_2O$) in measured PSmin, 8.6 (${\pm}3.25\;cmH_2O$) in calculated PSmin Correlation between the measured PSmin and the calculated PSmin is significantly high(n=9, r=0.88, p=0.002). The calculated PSmin show a tendancy to be higher than the corresponding measured PSmin in 8 out of 9 subjects(p=0.09). The ratio of measured PSmin/calculated PSmin was 0.81(${\pm}0.05$). Conclusion: Minimal pressure support levels were different in individual cases in the range from 4 to 12.5 cm$H_2O$. Because the equation-driven calculated PSmin showed a good correlation with measured PSmin, the application of equation-driven PSmin would be then appropriate compared with conventional application of 5~10 cm$H_2O$ in patients under difficult weaning process with pressure support ventilation.
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