• Fisheries and Aquatic Sciences
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• v.14 no.3
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• pp.174-178
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• 2011

This study aimed to determine the degree of hydrolysis and angiotensin-I-converting enzyme (ACE)-inhibitory activity of Giant Jellyfish Nemopilema nomurai (jellyfish) hydrolysates. The degree of hydrolysis using six proteolytic enzymes (Alcalase, Flavozyme, Neutrase, papain, Protamex, and trypsin) ranged from 13.1-36.8% and the inhibitory activities from 20.46-79.58%. Using papain hydrolysate, we newly isolated and characterized ACE-inhibitory peptides with a molecular weight of 3,000-5,000 Da that originated from jellyfish collagen. The purified peptide (FII-b) was predicted to be produced from an alpha-2 fragment of the type IV collagen of jellyfish. The N-terminal sequence of FII-b was Asp-Pro-Gly-Leu-Glu-Gly-Ala-His-Gly- and showed 87% identity to the collagen type IV alpha-2 fragment of Rattus norvegicus and a predicted protein from Nematostella vectensis, indicating that the ACE-inhibitory peptide originated from the collagen hydrolysate and had an $IC_{50}$ value of 3.8 ${\mu}g$/mL. The primary structure of the fragment is now being studied; this peptide represents an interesting new type of ACE inhibitor and will provide knowledge of the potential applications of jellyfish components as therapies for hypertension.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.44 no.2
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• pp.99-111
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• 2008

The purpose of this study is to develop the jellyfish separator system(JSS) for reducing fishery damage by the increase of jellyfish in the sea area of Korea in summer. First of all, to find the optimum structure of a JSS, six types of JSS in trawl fishery were designed and manufactured, the underwater shape of JSS and the separating process by JSS were observed in the circulating water channel(CWC). And the field experiments were carried out in July and September 2004 in the southern sea of Korea. For the moving path of the jellyfish model in the CWC, in case that the model was larger than the mesh size of the separator net, it was guided toward the lower part of the separator net by the guiding net and discharged through the outlet. In case that the model was smaller than the mesh size of the separator net, some models which passed through the upper part of the guiding net were smaller than the mesh size of the guiding net and discharged through the outlet and most of the model which passed through the lower part of the guiding net moved to the codend passing through the separator net. According to the field experiment result, the optimum tilt angle of separator net was inferred $20^{\circ}$ that the discharge rate of jellyfish was higher than the other tilt angle of separator net and the optimum structure of JSS was inferred GS type(consists of guiding net and separator net) that the discharge rate of jellyfish was higher than S type(consists of separator net). It was demamded to carry out more study for the countermeasure to reduce loss of fish.

• Korean Journal of Soil Science and Fertilizer
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• v.45 no.2
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• pp.227-234
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• 2012

Recently, the population of toxic and/or unusable jellyfish is increasing during summer along the east coast of Korea, causing massive economical and ecological damage to fisheries, nuclear power plant and marine environment. To solve this problem, this study was carried out using jellyfish as a potential soil additive for horticulture. The jellyfish was solidified and homogenized, then mixed with a commercial bed soil. Allium tuberosum ROTH was planted to control bed soil (BS) and jellyfish powder mixed bed soil groups (Mixed bed soil, MBS), and following parameters were measured during five weeks: water content, electrical conductivity and growth of leaves. At the end of the experiment, bacterial community structures of each pot were analyzed by DGGE. The relative water adsorption of jellyfish powder was about 2.5 times greater compared to its dry weight. The water content of MBS group was significantly higher than BS group 6.5 to 14.2%, and the electric conductivity of MBS group was measured around 2.8 dS/m where BS group was resulted average of 1.8 dS/m. However, the leaves of BS group were grown 30% longer compared to MBS group. DGGE analysis of MBS group was shown in high number of phylum Bacteroidetes and increased diversity of Sphingobacteriia compared to BS group. Jellyfish powder as a soil additive surely will be a good candidate as humectant and microbiota stimulator, although there are several obstacles such as high electrical conductivity and residual alum salt which used for solidification of jellyfish.

• Journal of Forest and Environmental Science
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• v.28 no.4
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• pp.227-231
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• 2012

Reforestation is one of the most important ways to reduce erosion soil. The objective of this study was to determine the effect of jellyfish soil amendment on seedling growth for reforestation. 100g jellyfish soil amendment was applied into planting hole for the purpose of improvement of the Chamaecyparis obtusa seedling growth. Results showed that during growing period, mortality were 4.4% for the fertilized group and 8.3% for control group. The seedling average height and root diameter were 95.0 cm and 1.07 cm in fertilized group and 40.6 cm and 0.74 cm in control group. The fertilized group was found to be superior (by 1 to 5%) to control group in terms of mortality rate, seedlings height, and root diameter. The positive growth of the fertilized group can be due the increase in soil moisture and the higher availability of nutrients to the plants from jellyfish fertilizer.

• Journal of Forest and Environmental Science
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• v.34 no.2
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• pp.196-198
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• 2018

This study aimed to evaluate the effectiveness of jellyfish chips in promoting the survival and growth of Pinus thunbergii seedlings in a coastal area based on a five-year field investigation from 2012 to 2016. Seedling survival rate was significantly higher in the treatment (96%) than in the control (75%) group in 2012. Furthermore, the height of five-year-old seedlings after plantation in 2012 was significantly greater in the treatment (3.41 m) than in the control (2.32 m) group (t=10.151; p-value<0.01). These results revealed that jellyfish chips can improve soil condition by enhancing moisture retention during the early growth stages and supplying nutrients to the seedlings over time. Our findings indicated that jellyfish chips could be used effectively as an organic fertilizer for growing coastal disaster prevention forests.

• Fisheries and Aquatic Sciences
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• v.27 no.1
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• pp.1-6
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• 2024

In this study, the swimming behavior of a small box jellyfish (Carybdea brevipedalia), which consists of inhaling external seawater and expelling it through its mouth to generate forward thrust, was characterized using particle image velocimetry (PIV). The flow rate and structure during the suction and discharge process were quantitatively analyzed. During swimming, there was a change in the ratio of the internal area during inhalation and expulsion. Specifically, there was a 1.10-fold difference between the maximum area after inhalation and the minimum area after discharge. The maximum distance traveled after discharge was inversely proportional to the size of the inner area, with a 2.48-fold difference in the minimum distance traveled after suction. Depending on the propulsion stage, the inner area decreased and then increased in proportion to the moving distance and speed. The moving distance of the small box jellyfish was measured for each period. The speed for each swimming stage increased and then decreased at intervals of 0.15 to 0.2 seconds, and the suction and discharge cycle period was measured at approximately 0.5 seconds. Collectively, our findings provide a methodological basis for studying the swimming behavior of small and highly active trailing jet jellyfish, as well as the biological mechanisms that determine this behavior.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2007.06a
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• pp.707-708
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• 2007

• Journal of Applied Biological Chemistry
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• v.62 no.4
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• pp.347-353
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• 2019

Stings of jellyfish, which frequently occur in a warm season, cause severe pain, inflammation and sometimes irreversible results such as the death. Harmful venoms from jellyfish, therefore, have been studied for finding the therapeutic agents to relieve pain or to neutralize toxic components. However, it is still unclear if and how jellyfish venom reveal neuronal toxicity even though pain induction seems to result from the activation of nociceptors such as nerve endings. In this study, using HT-22 cell line, we investigated neurotoxic effects of the venom of Chrysaora pacifica (CpV) which appears in South-East ocean of Korea. In 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay, CpV significantly reduced the viability of HT-22 cells in a dose-dependent manner. Additionally, in 2',7'-Dichlorofluorescin diacetate fluorescence test under the culture condition lacking dominant inflammatory factors, CpV remarkably increased the production of intracellular reactive oxygen species (ROS). Reduced responsive fluorescence to Rhodamine123 and increased expression of intracellular cytochrome c were also observed in HT-22 cells treated with CpV. These indicate that CpV-reduced viability of HT-22 cells may be due to the activation of apoptotic signalings mediated with oxidative stress and mitochondrial dysfunction. Furthermore, removing Ca²⁺ ion or adding N-acetyl-Lcystein remarkably blocked the CpV effect to reduce the viability of HT-22 cells. The findings in this study clearly demonstrate that CpV may activate Ca²⁺-mediated ROS signalings and mitochondrial dysfunction resulting in neuronal damage or death, and suggest that blocking Ca²⁺ pathway is a therapeutic approach to possibly block toxic effects of jellyfish venoms.

• Animal Systematics, Evolution and Diversity
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• v.33 no.1
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• pp.8-16
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• 2017

We firstly described a box jellyfish, Carybdea brevipedalia collected from the southern coasts of Korea. It is morphologically characterized by gastric phacellae, a special digestive system of cubozoan jellyfish, and velarium, the thin muscle flap forming the opening of the subumbrellar cavity. The phacellae are linear-shaped, comprising numerous cirri branched from multiple roots. Each root also has multiple numbers of cirrus bundles. Basis of velarial cannels parts into two branches in each octant of velarium. Its geographic distributions were limited to Jeju-do and the middle-southern coasts where the organisms are seriously hazardous to bathers in summer. Numerous individuals and/or large-sized populations were observed specifically from beaches at Jeju-do, Namhae-do and a small fishing port of Namildae, while only a small amount of the individuals was observed in Gamak Bay, Jaran-Goseung Bay, and Geoje-do. We confirmed molecular identity of the Korean C. brevipedalia with comparison of nuclear ribosomal DNA sequences. Until now, Carybdea brevipedalia is the only cubozoan species reported to be distributed in Korean waters.

• Natural Product Sciences
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• v.15 no.2
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• pp.71-75
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• 2009

The great economic and social damage caused by unusual explosion of jellyfish population has attracted the attention of researchers. A chemical study on the bioactive components of the giant jellyfish Nemopilema nomurai led to the isolation of two new (1 and 2) and three known alkoxyglycerols (3 - 5), along with known monoglycerides (6 - 7) and fatty acids. Based on NMR and MS data, the structures of compounds 1 and 2 were elucidated as 1-O-[(Z)-tetradec-3-enyl]-sn-glycerol and 1-O-[(Z)-octadec-10-enyl]-sn-glycerol, respectively. The absolute configurations of compounds 1 - 7 were determined by comparison of specific optical rotation values with those reported. The isolated compounds were evaluated for suppressive effect on the proinflammatory mediators (NO, IL-6, and TNF-${\alpha}$) in murine macrophage cells. However, they were found inactive upto the concentration of 100 ${\mu}M$.