• Journal of Veterinary Clinics
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• v.27 no.5
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• pp.501-507
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• 2010

The aim of the current study was to analyze the diversity of the major surface protein (Msp) gene in Theileria buffeli, which is known as the major antigenic protein recognized by the immune system of the host. In addition, we characterized the diversification of the Msp gene and its relationship to with the pathogenicity of Theileria. Complete blood counts (CBC) and Theileria 18S rRNA PCR sequence analysis were performed for 177 Korean indigenous cattle (KIC) in Jeju Island. A total of 28 KIC (16 anemic and 12 non-anemic KIC) were then randomly selected based on 18s rRNA PCR positive samples for sequence analysis of the Theileria Msp gene, which was performed twice for each specimen. The resulting 56 Msp gene sequences were classified into five antigenicity types (type I to V), according to the variable region (517-571 bp), which exhibited high similarity (${\geq}$ 98.9%) to several available GenBank sequences (Theileria spp. from China-EU584237; T. sergenti from China-DQ078264; Theileria spp. from Thailand-AB081329; Theileria spp. from Japan-AB218442; T. sergenti from Japan-AB016280). The 56 Msp sequences consisted of 22, 15, 9, 8, and 2 cases of type I to type V Msp genes, respectively. The most prevalent type in both anemic and non-anemic KIC was type I (37.5% in anemic and 41.7% in non-anemic). Among the remaining types, type II was the most prevalent (37.5%) in anemic KIC, while type IV was the most prevalent (25%) in non-anemic KIC. The results of our study help confirm the diversity of Msp gene types and demonstrate that the gene type distribution of Msp genes varies among Theileria-infected KIC in Jeju Island.

• Korean Journal of Agricultural and Forest Meteorology
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• v.8 no.2
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• pp.116-124
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• 2006

Remarkable winter season warming has been observed in East Asian countries during the last century. Accordingly, significant effects on dormancy and the resulting budburst of deciduous trees are expected. However phenological observations are rare and insufficient compared with the long-time climate records in the same region. A chill-day accumulation, which can be estimated from daily maximum and minimum temperature, is expected to make a reasonable proxy for dormancy depth of temperate zone fruit trees. To simulate dormancy depth during 1921-2004, a chill-day model parameterized for 'Campbell Early' grapevine, which is the major cultivar grown virtually anywhere in South Korea, was applied to daily temperature data at 8 locations in South Korea. The calculations showed that the chilling requirement for breaking endo-dormancy of this grapevine cultivar can be satisfied by mid-January to late February in South Korea, and the date was delayed going either northward or southward from the 'Daegu-Jeonju' line crossing the middle of South Korea in the east-west direction. Maximum length of the cold tolerant period (the number of days between endo-dormancy release and forced dormancy release) showed the same spatial pattern. When we divide the 83 years into 3 periods (I: 1921-1950, II: 1951-1980, and III: 1981-2004) and get the average of each period, dormancy release date of period III was accelerated by as much as 15 days compared with that of period I at all locations except Jeju (located in the southernmost island with subtropical climate) where an average15-day delay was predicted. The cold- tolerant period was also shortened at 6 out of 8 locations. As a result, budburst of 'Campbell Early' in spring was accelerated by 6 to 10 days at most locations, while inter-annual variation in budburst dates was increased at all locations. The earlier budburst after the 1970s was due to (1) warming in winter resulting in earlier dormancy release (Incheon, Mokpo, Gangneung, and Jeonju), (2) warming in early spring accelerating regrowth after breaking dormancy (Busan and Jeju), and (3) both of them (Seoul and Daegu).

• Microbiology and Biotechnology Letters
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• v.49 no.4
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• pp.552-558
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• 2021

Traditional foods are manufactured according to the characteristics of each region for the nations of the world. Korea has mainly farmed, and seasonings have developed around rice and vegetables. In fermented foods, lactic acid bacteria such as Lactobacillus sp. and Pediococcus sp. and Bacillus sp. were isolated and identified from fermented foods. In this study, lactic acid bacteria were isolated and identified from commercially available traditional Korean fermented foods, and candidate strains were selected through antibacterial activity tests on human and fish disease bacteria. Thereafter, the final strain was selected by examining the resistance to simulated gastric and intestinal fluids, and hemolysis. The three (M1, K1, C13) final selected latic acid bacteria were miced with prebiotics and the antibacterial activity of synbiotics was evaluated. As for the fist antibacterial activity result, C13 showed high antibacterial acitivity in human diseases and fish diseases. Then, M1, K1 and C13, which did not produce β-haemolysis and were resistant to simulated gastric and intestinal fluids, were subjected to the second antibacterial activity of synbiotics. When the three prebiotics (FOS, GOS, Inulin) and probiotics (M1, K1, C13) were mixed, the antibacterial activity was increased or inhibited. Based on the 16S rRNA gene sequencing results, K1 and M1 were analyzed as Bacillus tequiensis 99.72%, Bacillus subtilis 99.65%, Bacillus inaquosorum 99.72%, Bacillus cabrialesii 99.72%, Bacillus stercoris 99.58%, Bacillus spizizenii 99.58%, Bacillus halotolerans 99.58%, and Bacillus mojavensis 99.51%. And C13 was analyzed as Bacillus velezensis 99.71%, Bacillus nematocida 99.36%, Bacillus amyloliquefaciens 99.44%, Bacillus atrophaeus 99.22%, and Bacillus nakamurai 99.44%.

• Journal of the Korean Institute of Traditional Landscape Architecture
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• v.39 no.1
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• pp.37-42
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• 2021

Munseom (Island) is known as the largest native Elaeocarpus sylvestris var. ellipticus in Korea. Recently, disease damage from the E. sylvestris var. ellipticus community has been reported in Munseom. This study was conducted to understand the damage situation and growth characteristics of the E. sylvestris var. ellipticus population in Munseom. The purpose of this study was to evaluate the spontaneous regeneration potential of the community by grasping the characteristics of the population of surviving E. sylvestris var. ellipticus individuals, and to discuss ways to restore native habitats. A total population survey was conducted to determine the distribution status of E. sylvestris var. ellipticus individuals. The field survey carefully recorded the height of tree, DBH, DRH, and growing status, along with GPS location information of the individual. The growth status of E. sylvestris var. ellipticus individuals distributed in Munseom and the characteristics of tree height, DBH, DRH of each individual were analyzed. The total number of E. sylvestris var. ellipticus populations identified in Munseom was 293. The dense E. sylvestris var. ellipticus community reported in 2005 has been identified as being greatly damaged or damaged. In particular, the damage was more serious in mature trees with a height of 6m or more forming the canopy layer in the forest, and it was confirmed that 80.6% of the mature trees died. In the growth characteristics of the old-growth tree, which is an indicator of the health of the forest based on DRH, 75.4% died or the growth condition was found to be poor. In order to restore the native habitat of E. sylvestris var. ellipticus in Munseom, conservation efforts such as pest control, preservation of mature trees, control of the territory of young trees, and reintroduction after ex-situ transplantation etc. are considered necessary.

• The Journal of Engineering Geology
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• v.33 no.1
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• pp.105-119
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• 2023

Water supply is decreasing due to climate change, and coastal and island regions are highly dependent on groundwater, reducing the amount of available water. For sustainable water supply in coastal and island regions, it is necessary to accurately diagnose the current condition and efficiently distribute and manage water. For a precise analysis of the groundwater flow in the coastal island region, submarine fresh groundwater discharge was calculated for the Seongsan basin in the eastern part of Jeju Island. Two methods were used to estimate the thickness of the fresh groundwater. One method employed vertical interpolation of measured electrical conductivity in a multi depth monitoring well; the other used theoretical Ghyben-Herzberg ratio. The value using the Ghyben-Herzberg ratio makes it impossible to accurately estimate the changing salt-saltwater interface, and the value analyzed by electrical conductivity can represent the current state of the freshwater-saltwater interface. Observed parameter was distributed on a virtual grid. The average of submarine fresh groundwater discharge fluxes for the virtual grid was determined as the watershed's representative flux. The submarine fresh groundwater discharge and flux distribution by year were also calculated at the basin scale. The method using electrical conductivity estimated the submarine fresh groundwater discharge from 2018 to 2020 to be 6.27 × 10⁶ m³/year; the method using the Ghyben-Herzberg ratio estimated a discharge of 10.87 × 10⁶ m³/year. The results presented in this study can be used as basis data for policies that determine sustainable water supply by using precise water budget analysis in coastal and island areas.

• Analytical Science and Technology
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• v.20 no.5
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• pp.383-392
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• 2007

The water soluble components were analyzed in the $PM_{2.5}$ fine particles collected at the 1100 site of Mt. Halla for the spring seasons between 1998 and 2004. The $PM_{2.5}$ mass concentrations were within $13.4{\pm}9.6{\sim}21.7{\pm}20.0{\mu}g/m^3$, and the concentrations of ionic components were in the order of nss-$SO{_4}^{2-}$ > $NH{_4}{^+}$ > $NO{_3}{^-}$ > $Ca^{2+}$ > $K^+$ > $Na^+$ > $Cl^-$ > $Mg^{2+}$, in which the concentration of nss-$SO{_4}^{2-}$($3.41{\pm}2.42{\mu}g/m^3$) was the highest. The concentrations of $NH{_4}{^+}$, $SO{_4}^{2-}$, and $NO{_3}{^-}$, the secondary pollutants, were respectively 0.60~1.50, 2.86~4.42, and $0.24{\sim}1.57{\mu}g/m^3$, which had occupied 88 % of the total ionic components, on the other hand, the concentrations of marine species were less than 5 %. The nss-$SO{_4}^{2-}$ showed the high correlation with $NH{_4}{^+}$, $K^+$, so that $NH{_4}{^+}$ and nss-$SO{_4}^{2-}$ might exist in the form of $(NH_4)_3H(SO_4)_2$ and $(NH_4)_2SO_4$ in fine particles. From the backward trajectory analysis, in case of high concentrations of $NH{_4}{^+}$ and nss-$SO{_4}^{2-}$ simultaneously, the air masses were originated and stagnated at the east region of China for a while, then moved into the atmosphere of Jeju. However, in case of $NO{_3}{^-}$ and nss-$Ca^{2+}$, the air masses originated at China and Siberia were moved into Jeju via the eastern China.

• Korean Journal of Environmental Biology
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• v.35 no.1
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• pp.13-20
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• 2017

Greenhouse gas emission from agricultural land is recognized as an important factor influencing climatic change. In this study, the national $CO_2$ emission was estimated for paddy soils, using soil GHG emission model (DNDC) with $1km^2$ scale. To evaluate the applicability of the model in Korea, verification was carried out based on field measurement data using a closed chamber. The total national $CO_2$ emission in 2015 was estimated at $5,314kt\;CO_2-eq$, with the emission per unit area ranging from $2.2{\sim}10.0t\;CO_2-eq\;ha^{-1}$. Geographically, the emission of Jeju province was particularly high, and the emission from the southern region was generally high. The result of the model verification analysis with the field data collected in this study (n=16) indicates that the relation between the field measurement and the model prediction was statistically similar (RMSE=22.2, ME=0.28, and $r^2=0.53$). More field measurements under various climate conditions, and subsequent model verification with extended data sets, are further required.

• Journal of Environmental Science International
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• v.15 no.3
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• pp.271-278
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• 2006

To investigate the variations of physico-chemical factors and microbial population, in ten stations at water region of coastal area of Chagwi-Do, Nutritive salts, water temperature, transparency, suspended solid, salinity, COD, DO, pH, heterotrophic bacteria, coliform group and Vibrio spp. were analysed three times in September, November in 2004 and February in 2005. Heterotrophic bacteria in surface water was $3.5X10^1{\sim}1.16X10^3cfu/ml,\;1.0X10^2{\sim}5.2X10^1cfu/ml\;2.0X10^1{\sim}7.6X10^1cfu/ml$ and bottom water counted $7.0X10^2{\sim}1.0X10^3cfu/ml,\;1.4X10^1{\sim}2.5X10^2cfu/ml\;2.0X10^2{\sim}4.2X10^1cfu/ml$ in September, November in 2004 and February in 2005, respectively. The cell number of total coliform bacteria in the surface water amounted to $0{\sim}4.3X10^2cfu/ml,\;0{\sim}6.0X10^1cfu/ml,\;0{\sim}1.0X10^1cfu/ml$ and bottom water amounted $0{\sim}2.2X10^2cfu/ml,\;0{\sim}5.4X0^2cfu/ml,\;0{\sim}2.0X10^1cfu/ml$ in September, November in 2004 and February in 2005, respectively. As for Vibrio spp., the cell number in the surface water was $1.0X10^1{\sim}2.5X10^2cfu/ml,\;1.0X10^1{\sim}2.0X10^1cfu/ml,\;0cfu/ml$ and bottom water counted $1.0X10^1{\sim}5.2X10^2cfu/ml,\;0cfu/ml,\;2.0X10^1cfu/ml$ in September, November in 2004 and February in 2005, respectively.

• Journal of Korean Society of Forest Science
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• v.105 no.1
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• pp.63-72
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• 2016

A study was conducted to evaluate the effect of collected region(Bongpyeong in Gangwon, 690 m, Mt. Halla in Jeju, 1,085 m), pre-treatment (stratification, soaked in distilled water or $GA_3$) and shading (control, 50%, 80%) on seed emergence and seedlings growth of Ligularia fischeri. The result showed that seed emergence (%) of L. fischeri were 6.9~75.5% (Gangwon : 6.9~32.8%, Halla : 22.2~75.5%). Maximum seed emergence (%) of 32.8 (Gangwon) and 75.5 (Halla) were achieved for seeds were stratified, soaked in distilled water, and under non-shading for L. fischeri (Gangwon), whereas seeds were stratified, soaked in $GA_3$ 100 ppm, and under 80% shading for L. fischeri (Halla). In this study, the best seedling vigor index (SVI) of L. fischeri (Gangwon) and L. fischeri (Halla) were obtained when seeds were stratified, soaked in distilled water, and under 50% shading. The survival rate of potted seedlings for L. fischeri (Halla) were 100% of all shading conditions, but there were decreased by 77.8%, 61.1%, 55.6%, as an increasing shading rate (con, 50%, 80%) for L. fischeri (Gangwon). Therefore, good seedlings were obtained when seed (Mt. Halla) of L. fischeri were stratified, soaked in distilled water, and under 50% shading, and then seedlings were transferred in pot.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.15 no.1
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• pp.26-34
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• 1982

The East China Sea and the Yellow Sea are abundant in nutritions because of river inflows and are important as the nursery and spawning grounds of demersal and pelagic fishes. The remarkable thermal front between the Yellow Sea Bottom Cold Water and the Tsushima Warm Current is formed in this region, and the fluctuation of this front may affect the variation of the yellow croaker fishing ground. To investigate the mechanism of the yellow croaker fishing ground, the distribution ana seasonal change of the fishing ground are examined by using catch of stow net fishery (Fisheries Research and Development Agency, 1970-1979) and the water temperature data (Japan Hydrographic Association, 1978). The main fishing ground of yellow croaker was nine sea areas (rectangle of 30' latitude by 30' longitude) located at 40-150 nautical miles west and southwest of Jeju Island, the area of which occupies no more than $11\%$ of all fishing grounds, and it appeared that about $70\%$ of total catch of ten years was concentrated in this area. The main fishing periods were from March to May and September to October. The coefficients of variation of the catch for the main fishing ground were from 0.8 to 2.1 and the condition of all fishing grounds was generally unstable. The mean CPUE was 27kg/haul at the main fishing ground, while it was the largest on boundary area of the Yellow Sea Bottom Cold Water. It was found that the seasonal movement of fishing ground is related to the expansion and reduction of the Yellow Sea Bottom Cold Water ($10^{\circ}C$).