• 한국임상수의학회지
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• 제22권4호
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• pp.386-391
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• 2005

This study was done to produce a mutated protein inactivated cytotoxicity of Shigatoxin 2e (Stx2e) of E.coli O139 isolates by deletional mutagenesis of Stx2e A subunit gene encoding active-site cleft of enzymatic domain in ST2e holotoxin. Cytotoxicity of the toxoid expressed from the mutant Stx2e gene was compared with wild type Stx2e for development of vaccine candidate. A recombinant plasmid pED18 containing Stx2e gene ot E.coli O139 isolates was used to generate mutation plasmid. Deletion mutagenesis was conducted for Stx2e A subunit gene encoding enzymatically active domain by polymerase chain reaction (PCR) using ot designed primer to induce deletional mutation. DNA sequence analysis was confirmed that the pentamer (Typ 202- Ser 206) that lies within the proposed active-site cleft in the second region was completely deleted. A DNA fragment of 1.1 kb that encode the new mutant Stx2eA gene was inserted into plasmid pRSET vector digested with EcoRV-Hind III and named pEDSET The PEDSET was transformed in E. coli for expression of mutant protein and the protein was confirmed by SDS-PACE and Western-blotting. The protein expressed by the mutant was tested to confirm the reduction of cytotoxic activities on Vero cell using microcytotoxicity assay compared with wild type Stx2e, the cytotoxicity of deletional mutant protein was at least reduced by 3,000-fold on Vero cell.

• 한국보건간호학회지
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• 제16권2호
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• pp.304-314
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• 2002

A survey was conducted from September 15 to December 15 2001. Structured questionnaires were mailed to school nurses in 607 elementary schools around the country in order to determine the current status of anti-smoking education in elementary schools. The result were as followers 1. Of the 607 schools, 535 $(88.1\%)$ offered smoking-prevention education. By region, Jeju-do $(100\%)$ topped the list, followed by Seoul $(90.3\%)$, Gyeongsang-do$(90.2\%)$, Jeolla-do$(88.9\%)$, Gangwon-do $(87.8\%)$, Chungcheong-do $(84.6\%)$, and Gyeonggi-do $(81.4\%)$. 'Recognition of the need for anti-smoking program $(86\%)$' was a major motivation for initiating the program, while 'too much workload $(46.4\%)$' was cited as a main reason for the failure to do so. 2. The classes were offered mostly for 6th-grade students $(87.8\%)$, while $9.0\%$ and $2.0\%$ were implemented at 5th- and 4th- grades, respectively. 3. $49.1\%$ of the classes offered lasted one hour, while $31.8\%$ involved a two-hour program. 4. Programs were mainly about smoking-related diseases, habitual nature of smoking, impediment to growth and development, etc. 5. Audio-visual lecture $(46.5\%)$ was most frequently used as a method of education, followed by lecture. 6. $72.7\%$ of the programs used classroom as a unit of education, while collective education by sex or by grade accounted for $22.6\%$. 7. Video $(51.0\%)$ was the most popular medium for education, while computer ranked second with $26.5\%$. 8. $92.5\%$ of the education was offered by school nurses. 9. $99.2\%$ of school nurses responded in favor of anti-smoking programs. with $60.1\%$ of them answering that such education is a must. 5th grade was the most commonly cited grade for the initiation of the programs, followed by 4th grade and 6th grade. $33.2\%$ picked two hours as the most appropriate length of the program at the elementary school level. while $25.1\%$ chose 3 hours out of the range of 1-11 hour(s). 10. With regard to the evaluation by school nurses on smoking-prevention program, more than $30\%$ felt that hours of education, education materials, medium of education, interests of other teachers, interests of school authorities, etc. were inadequate or insufficient.

• Asian-Australasian Journal of Animal Sciences
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• 제31권11호
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• pp.1691-1699
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• 2018

Objective: In Korea, there are three main cattle breeds, which are distinguished by coat color: Brown Hanwoo (BH), Brindle Hanwoo (BRH), and Jeju Black (JB). In this study, we sought to compare the genetic diversity and divergence among there Korean cattle breeds using a BovineHD chip genotyping array. Methods: Sample data were collected from 168 cattle in three populations of BH (48 cattle), BRH (96 cattle), and JB (24 cattle). The single-nucleotide polymorphism (SNP) genotyping was performed using the Illumina BovineHD SNP 777K Bead chip. Results: Heterozygosity, used as a measure of within-breed genetic diversity, was higher in BH (0.293) and BRH (0.296) than in JB (0.266). Linkage disequilibrium decay was more rapid in BH and BRH than in JB, reaching an average $r^2$ value of 0.2 before 26 kb in BH and BRH, whereas the corresponding value was reached before 32 kb in JB. Intra-population, interpopulation, and Fst analyses were used to identify candidate signatures of positive selection in the genome of a domestic Korean cattle population and 48, 11, and 11 loci were detected in the genomic region of the BRH breed, respectively. A Neighbor-Joining phylogenetic tree showed two main groups: a group comprising BH and BRH on one side and a group containing JB on the other. The runs of homozygosity analysis between Korean breeds indicated that the BRH and JB breeds have high inbreeding within breeds compared with BH. An analysis of differentiation based on a high-density SNP chip showed differences between Korean cattle breeds and the closeness of breeds corresponding to the geographic regions where they are evolving. Conclusion: Our results indicate that although the Korean cattle breeds have common features, they also show reliable breed diversity.

• 한국수산과학회지
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• 제52권6호
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• pp.666-684
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• 2019

The horizontal distributions of eggs and larvae of chub mackerel Scomber japonicus were extensively surveyed in the vicinity of Korean waters between 31°75'N and 36°50'N during May and June in 2016 and 2017 (total of four surveys). We used a coupled bio-physical model (DisMELS) that combines an individual-based model (IBM) incorporating vertical migration of larvae and temperature-dependent survival to understand transport processes in the early life stage. Using the distributions of eggs and larvae from surveys, the potential spawning grounds were estimated at the northwest and southeast of Jeju Island and the central East China Sea in May, and at the southwestern East Sea and southern West Sea in June by running the model backward in time. In forward experiments within 30 days from the backward results, most larvae were transported to both the Korean and Japanese sides of the East Sea through the Korea Strait. However, the larvae released in the central East China Sea were transported to the Japanese side only, while those released in the southern West Sea were retained within that region. The survival rates at 30 days after release based on the simulation incorporating temperature-dependent survival throughout May and June were 29.7% in 2016 and 28.8% in 2017.

• 환경생물
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• 제25권4호
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• pp.336-341
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• 2007

2004년 9월, 11월 그리고 2005년 2월 총 3회에 걸쳐 제주 차귀도 연안해역 10개 정점 표층수와 저층수에서 종속영양세균의 개체수를 조사하였고, 또한 해당 해역 수질의 이화학적 특성을 조사하였다. 2004년 9월, 11월 그리고 2005년 2월에 종속영양세균의 수는 각각 표층수에서는 $3.5\times10^1\sim1.16\times10^3\;cfu\;mL^{-1}$, $0.4\times10^1\sim5.6\times10^1\;cfu\;mL^{-1}$, $0.4\times10^1\sim7.8\times10^1$ 그리고 저층수에서는 $4.5\times10^1\sim1.0\times10^3\;cfu\;mL^{-1}$, $1.2\times10^1\sim1.5\times10^2\;cfu\;mL^{-1}$, $0.4\times10^1\sim4.4\times10^1\;cfu\;mL^{-1}$가 검출 되었다. 또한 조사해역의 수온, pH, 염분 및 COD, DO, 총 인, 총 질소, 투명도 그리고 부유물질을 분석하여 보았는데 수온이 높은 2004년 9월에 그 수치가 조금 높았을 뿐 해역수질 환경기준에 있어 1등급에 만족하는 결과가 나타났다. 2004년 9월, 11월, 2005년 2월 각 정점별 표층수와 저층수에서 우점적으로 자란 colony를 순수 분리하여 16S rRNA 염기서열 분석을 통하여 종속영양세균의 우점종을 비롯한 종속영양세균 군집을 분석한 결과 표층수와 저층수에서 Vibrio spp.와 Pseudoalteromonas spp.가 우점종으로 나타났으며 그 외에 Psuedomonas spp, Bacillus spp., Alteromonas spp., Aeromonas spp., Psychrobacter spp., Flavobacterium spp.가 조사 되었다.

• Parasites, Hosts and Diseases
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• 제52권3호
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• pp.281-285
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• 2014

Ticks were collected from 35 animals from 5 provinces and 3 metropolitan cities during 2012. Ticks also were collected by tick drag from 4 sites in Gyeonggi-do (2) and Jeollabuk-do (2) Provinces. A total of 612 ticks belonging to 6 species and 3 genera were collected from mammals and a bird (n=573) and by tick drag (n=39). Haemaphyalis longicornis (n=434) was the most commonly collected tick, followed by H. flava (158), Ixodes nipponensis (11), Amblyomma testudinarium (7), H. japonica (1), and H. formosensis (1). H. longicornis and H. flava were collected from all animal hosts examined. For animal hosts (n>1), the highest Tick Index (TI) was observed for domestic dogs (29.6), followed by Siberian roe deer (17.4), water deer (14.4), and raccoon dogs (1.3). A total of 402 H. longicornis (adults 86, 21.4%; nymphs 160, 39.8%; larvae 156, 38.9%) were collected from wild and domestic animals. A total of 158 H. flava (n=158) were collected from wild and domestic animals and 1 ring-necked pheasant, with a higher proportion of adults (103, 65.2%), while nymphs and larvae only accounted for 12.7% (20) and 22.2% (35), respectively. Only 7 A. testudinarium were collected from the wild boar (6 adults) and Eurasian badger (1 nymph), while only 5 I. nipponensis were collected from the water deer (4 adults) and a raccoon dog (1 adult). One adult female H. formosensis was first collected from vegetation by tick drag from Mara Island, Seogwipo-si, Jeju-do Province.

• 한국산업보건학회지
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• 제24권3호
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• pp.293-299
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• 2014

Objectives: The main objective of this study is to investigate domestic usage amount of insecticide for vegetable cultivation to provide fundamental data for establishing job-exposure matrix(JEM) related to farmers treating agricultural insecticide. Materials and Methods: The survey on domestic usage amount of insecticide for vegetable cultivation was conducted by two research methods. The first method is to utilize agricultural pesticides published annually from Korea Crop Protection Association(KCPA). The second method is to apply cultivation area of vegetable announced officially from Statistics Korea(SK). An estimation of domestic usage amount of insecticide for vegetable cultivation through the second method was done by multiplying total cultivation area of vegetable($m^2$) with optimal spray amount of insecticide for vegetable cultivation per unit cultivation area of vegetable ($kg/m^2$). Results: As a result of analysis of public data related to insecticide for vegetable cultivation, it was found that its domestic usage amount has decreased gradually from the first sale year(1969) to current year(2012). There is, however, a considerable difference of annual usage trend of insecticide for vegetable cultivation between shipments and estimation. The annual usage trends of insecticide for vegetable cultivation based on regional classification were different from those based on total aspect. Conclusions: The region which used insecticide for vegetable cultivation the most in Korea was Jeolla-do, followed by Gyeonsang-do, Chungcheong-do, Seoul/Gyeonggi-do, Gangwon-do and Jeju-do. Substantially, mean ratio of usage amounts of insecticide based on shipments and those based on estimation by cultivation area was $281{\pm}115%$, which indicates that usage amounts of insecticide estimated by cultivation area are three times lower than those based on shipments.

• 한국산업보건학회지
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• 제24권1호
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• pp.59-64
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• 2014

Objectives: The principal aim of this study is to investigate and analyze domestic usage amounts of insecticide in rice cultivation in order to provide fundamental data for establishing a job-exposure matrix(JEM) related to farmers working with agricultural insecticides. Materials and Methods: An investigation of domestic usage amounts of insecticides rice cultivation was performed through two methods. The first method utilized information on agricultural pesticides published annually by the Korea Crop Protection Association(KCPA). The second method made use of area of cultivation of rice as officially determined by Statistics Korea(SK). An estimation of domestic usage of insecticides in rice cultivation through the second method was determined by multiplying the total cultivation area of rice($m^2$) by the optimal spray volume of insecticides for rice cultivation per unit of cultivation area($kg/m^2$). Results: As a result of the analysis of public data regarding insecticides in rice cultivation, it was found that the domestic usage amount has decreased sharply from the first year of market sales(1969) to the final data year(2012). There is little difference in the annual usage trend of insecticides in rice cultivation between shipment and estimation. Also, the annual usage trends of insecticides in rice cultivation based on regional classification were nearly similar to those based on the overall aspect. Conclusions: The region which used the largest volume of insecticide in rice cultivation in Korea was the Jeolla Provinces, followed by the Gyeonsang Provinces, the Chungcheong Provinces, Seoul/Gyeonggi Province, Gangwon Province and Jeju Province. Substantially, the mean ratio of usage amounts of insecticide based on shipments and those based on estimation by cultivation area was $96{\pm}29%$, which indicates that the domestic usage amount of insecticide for rice cultivation corresponded to the optimal spray standard per unit area.

• 한국지역지리학회지
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• 제17권4호
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• pp.416-431
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• 2011

지리산과 한라산을 대상으로 산악 국립공원 등산로의 훼손 유형을 요인별로 조사하였다. 등산로 훼손은 침식프로세스가 진행되는 장소에 따라 노면 저하, 측벽 후퇴, 노면 확대 및 분기로 구분할 수 있다. 노면 저하와 측벽 후퇴가 주로 자연적인 침식 요인에 의해 일어나는 훼손 유형인 반면 노면 확대 및 분기는 인위적인 답압으로 발생하는 훼손 유형이다. 노면 저하를 일으키는 가장 탁월한 프로세스는 우세이며, 측벽 후퇴는 등산로의 물리적 환경에 따라 우세, 서릿발작용, 취식, 도목, 동물작용 등 탁월한 프로세스가 달라진다. 노면 확대 및 분기는 탑압을 일으키는 원인에 의해 유형화할 수 있다. 지표류에 의해 등산로 노면이 낮아지는 과정에서 드러난 나무뿌리, 자갈 및 암반과 국지적인 세굴로 인하여 노변에 만들어진 높낮이차 등 우세와 관련하여 발생하는 훼손 사례가 많다. 또한 평탄면 등산로에서는 물웅덩이, 산림지대의 등산로에서는 천근성 수목인 구상나무의 도목이 노면 확대 빛 분기의 주요 원인이 된다. 돌깔기 공법처럼 노변 보호공법이 오히려 등산객의 등산로 이탈을 조장하기도 하며, 보행에 불편을 주는 요소가 없더라도 등산객이 지름길을 만드는 과정에서 노면 확대 및 분기가 발생할 수 있다.

• 자원환경지질
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• 제17권3호
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• pp.215-230
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• 1984

Petrological, paleomagnetic, geomorphological and structural studies on the southern part of, so called, Chugaryeong rift valley, have been carried out in order to clarify the nature of the rift valley. Three stages of volcanic activities characterized by Jijangbong acidic volcanic rocks and tholeiitic and andesitic basalt of Cretaceous age(?), and Jongok Quaternary olivine basalt occurred along the Dongducheon fault line. Jijangbong acidic volcanic rocks distributed in the central part of the studied area consist of rhyodacite, acidic tuff and tuff breccia, which are bounded by Dongsong fault on the east and Daegwangri fault on the west. The Jongok basalt differs from those of Ulrung and Jeju islands in mineralogy, chemical composition and differentiation. Jongok basalt distributed along the Hantan river dilineates the vesicles curved toward downstream direction and increment of numbers and thickness of lava flow toward upstream direction. These facts suggest that lava flowed from upstream side of the river. Rectangular drainage patterns also support the presence of the Dongducheon, Pocheon, Wangsukcheon and Kyonggang faults which were previously known. LANDSAT image, however, does not show any lineaments which could be counted as a graben or rift valley. Displacement of Precambrian quartzite and Jurassic Daedong supergroup along the southwestern extension of the Dongducheon fault shows the right lateral movement. The Paleomagnetic study of the tholeiitic and andesitic basalts from Baegeuri, Jangtanri and Tonghyeonri located at 2. 3km east, 0km east, and 1.5km west of Dongducheon fault respectively shows that their VGP(Virtual Geomagnetic Pole) being to intermediate geomagnetic field of short duration which suggests that they formed in almost same period. Mean VGP of Jongok basalt is located 82.4N and 80.6E. This is in good coincidence with worldwide VGP of Plio-Pleistocene indicating that Jongok basalt was extruded during Plio-Pleistocene epoch, and suggesting that the studied area has been tectonically stable since then. From the present study, the tectonic episode of the region is concluded as following three stages. 1. The 1st period is worked by the Daebo orogeny of Jurassic during which granodiorite was intruded in Precambrian basement. 2. The 2nd period is the time when right lateral strike-slip fault of NNE-SSW direction was formed probably during late Cretaceous to Paleogene and the Jijangbong acidic volcanic rocks and the older basalts were extruded. 3. The 3rd period is the time when the fault was rejuvenated during Pliocene or Pleistocene accompanied by the eruption of Jongok basalt. As a conclusion, geologic structure of the studied area is rather fault line valley than graben or rift valley, which is formed by differential erosion along the Dongducheon fault suggesting a continuation of the Sikhote-Alin fault. The volcanic rocks including the Jijangbong acidic rocks, tholeiitic-andesitic basalt and olivine basalt are associated with this fault line.