• Journal of Microbiology and Biotechnology
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• v.5 no.5
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• pp.285-291
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• 1995

Saccharide utilizations for the growth by Bifidobacterium longum and Bifidobacterium breve were compared. B. longum fermented glucose more rapidly than lactose as a carbon source whereas B. breve fermented lactose at a rate higher than that of glucose. The highest cell concentration, in the case of B. longum, was obtained when cultivated in a jar fermentor that contained modified MRS medium that half the beef extract was replaced by the same amount of tuna extract, and that pH was controlled at 6.0. B. breve showed the best growth when grown in a jar fermentor containing the MRS medium with lactose instead of glucose, controlled at pH 6.0. The optimal concentration of peptone in MRS medium for the growth of B. breve was 5 g/l.

• Microbiology and Biotechnology Letters
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• v.30 no.2
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• pp.167-171
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• 2002

The resting cells of Candida sp. SY16 produced a large amount of mannosylerythritol lipid as a biosurfactant when incubated in the distilled water containing only the carbon source. The resting cells exhibited the highest production at 20 g cells per liter on the soybean oil of 75 g/1 as a sole substrate and pH 4∼5 in the shaking culture. Under the optimal conditions, the biosurfactant was extracellularly produced to 58 g/1 after 120 h in jar fermentor, and the yield became higher than that obtained by using the glowing cells of the strain in batch fermentation.

• Korean Journal of Food Science and Technology
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• v.23 no.3
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• pp.291-295
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• 1991

In order to obtain the highest productivity of polysaccharide, acetic acid bacteria was used. Several acetic acid bacteria were investigated to the productivity of polysaccharide, an mutant, Acetobacter pasteurianus IFO 13751-5 selected among serveral acetic acid bacteria which can produce the polysaccharide by radiation of ultra-violet ray. Acetobacter pasteurianus IFO 13751-5 was shown 3 fold polysaccharide production than that of its parents. When the Acetobacter pasteurianus IFO 13751-5 was investigated under the condition of carbon source containing 5% sucrose, the highest amount of polysaccharide (45.95 mg/ml) was obtained. The polysaccharide production by Acetobacter pasteurianus IFO 13751-5 was 55.10 mg/ml by using jar fermentor.

• Korean Journal of Food Science and Technology
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• v.35 no.3
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• pp.417-422
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• 2003

The onions are considered to be a favorable functional source of beverage because they contain much sugar and various nutrients, and they are juicy vegetable. Recently, consumers have a new trend to take functional foods with health benefits. To meet this need, this study was the basic research to establish a manufacturing process of functional onion beverage by glucose oxidase. Glucose oxidase catalyzes reaction of glucose oxidation and makes generation of gluconic acid. Kinetics of the reaction was also investigated, and maximum glucose consumption rate $(V_{max})$ of $26.1{\times}10^{-2}\;g/L{\cdot}min$ and $K_m$ of 5.84 g/L were obtained. Optimum conditions were obtained when the glucose oxidase catalyzed reaction was carried out at temperature of $25^{\circ}C$, agitation rate of 450 rpm and aeration rate of 4 vvm in a 2.5 L jar fermentor. Finally, the enzyme reactor was 10-times scaled up and a similar glucose oxidation performance was achieved in the scaled-up reactor.

• Microbiology and Biotechnology Letters
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• v.25 no.6
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• pp.598-605
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• 1997

The characteristics of cell growth and cellulose production by Acetobcter xylinum BRC5 were studied in shaking flasks and jar fermentors. Of the substrates tested, the highest cellulose yield was obtained from the fructose medium. Some inductive cultivation was observed and then cellulose was produced with cell growth. When glucose alone or mixture of glucose and fructose was used as the carbon source, cellulose could be biosynthesized under the glucose limitation. Corn steep liquor (CSL), as a low-cost nitrogen source, was a good substitute for yeast extract. In a jar fermentor experiment, 4.14 g/l of disintegrated cellulose was obtained from 8% CSL- medium containing 0.5% glucose and 1.5% fructose.

• 한국생물공학회:학술대회논문집
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• 2000.04a
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• pp.211-214
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• 2000

Marine Microorganism strain 96CJ10356 produced extracellular polysaccharide (EPS-R) accompanied with cell growth. To improve the production of EPS-R, the effect of aeration rate was tested in a 5-liter jar fermentor with STN medium. The production of EPS-R was increased with aeration rate and after 72 hour cultivation, 12.20 g/l of EPS-R was obtained with an aeration rate of 1.5 vvm and the apparent viscosity was measured to be about 1000 cp with culture broth.

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 1976.10a
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• pp.186.3-187
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• 1976

섬유소단세포단백의 공업적 생산을 위하여 실험적인 결과를 토대로하여 Jar Fermentor에 의한 scale up 실험을 하였고, pilot plant scale의 발효조를 이용하여 대량생산 실험을 하였는 바 그 결과를 발표하고자 한다. 1. 배양중 생성되는 유기산을 중화하기 위하여 주입되는 암모니아의 소비량과 균체농도는 배양종반을 제외하고는 거의 비례하였으며 암모니아소비량으로 균체생육 정도를 간접적으로 판단할 수 있었다.(중략)

• Korean Journal of Microbiology
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• v.32 no.3
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• pp.232-237
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• 1994

Microorganism producing glutaryl 7-aminodeacetoxycephalosporanic acid (GL-7-ADCA) acylase was screened from soil. The microorganism was identified as Alcaligenes sp. J-421 by its morphology and biochemical properties. Cultural conditions of Alcaligenes sp. J-421 were investigated for the production of GL-7-ADCA acylase. Optimum medium composition was 1% glucose, 1% beef extract, 0.5% yeast extract, 0.2% monosodium L-glutamate, 0.1% glutaric acid, 0.2% NaCl, 0.5% $K_2$ $HPO_4$, and 0.05% $CuSO _4{\cdot}5H_2O$. Optimum cultivation conditions for the production of the enzyme in 5 l jar fermentor were $37^{\circ}C$, tip speed 300 rpm, aeration 1 vvm. Optimum reaction pH of the enzyme was 8.0 and the enzyme was stable at pH7.0-11.0.

• Microbiology and Biotechnology Letters
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• v.14 no.6
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• pp.461-466
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• 1986

A highly cyclodextrin glucanotransferase producing strain of Bacillus sp. was isolated from soil, and basic studies on the characteristics of the strain and its enzyme, conditions for the enzyme production, and the enzyme utilization were carried out. The isolated strain was aerobic, motile, endospore-forming and rod-shaped bacterium. Optimum pH and temperature for the enzyme action were 6.0 and 45$^{\circ}C$, and the enzyme was stable within 5$0^{\circ}C$, and between pH 6.0 and 10.0. The highest yield of the enzyme was obtained using the medium containing 2% corn starch as a carbon source, and 5% corn steep liquor, 0.1% urea and 0.25% ammonium sulfate as nitrogen sources. The fermentation conditions for the enzyme production in a jar fermentor were cetermined to be 3$0^{\circ}C$, 200rpm, 0.6vvm and 60hr cultural period. Stevioside transglycosylation catalyzed by this enzyme was identified by high performance liquid chromatography.

• Microbiology and Biotechnology Letters
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• v.20 no.4
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• pp.477-482
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• 1992

A bacterial strain Acinetobacter calcoaceticus was examined for its ability to degrade fats, oils and hydrocarbons, and tested for the possibility of application in wastewater treatment. All fats and oils tested were degraded by the strain. About 60% of hexadecane, 26% of fish oiL and 40-54% of vegetable oils were consumed respectively in shaking-flask culture. Saturated fatty acid compositions were about 55% in fish oil and 6-12% in vegetable oils. Increases in cell mass were accompanied with decreases in the concentrations of carbon sources. When jar fermentor in place of shaking-flask was used as a culturing vessel. above 80% of all carbon sources was consumed and yield of cell mass was improved to nearly 1.00. Synthetic wastewaters containing 3% of fat, oil, or hydrocarbon as a sale ca,bon source were treated sequentially with A. calcoaceticus first and then exposed to activated sludge. The concentrations of carbon sources were decreased below 0.06% through the process, and the concentrations of suspended solids were lower than 53 mglml. The data imply the potential use of A. calcoaceticus in wastewater treatment.