With the introduction of DNA microarrays, a high throughput analysis of gene expression is now possible as a replacement to the traditional time-consuming Southern-blot analysis. This cDNA microarray should be ahighly favored technology in the area of molecular toxicology or analysis of environmental stresses.In this study, therefore, we developed a novel cDNA microarray for analyzing stress-specific responses in japanese Medaka fish. In the design and fabrication of this stress specific functional cDNA microarray, 123 different genes in Medaka fish were selected from eighteen different stress responsive groups and spotted on a 25${\times}$75 mm glass surface. After exposure of the fish to bisphenol A which is the one of the well-known endocrine disrupting chemicals (EDCs), over 1 or 10 days, the responses of the DNA chip were found to show distinct expression patterns according to the mode of toxic actions from environmental toxicants. As a results, they showed specific gene expression pattern to bisphenol A, additionally, the chemical spesific biomarkers could be suggested based on the chip analysis data. Therefore, this chip can be used to monitor stress responses of unknown and/or known toxic chemicals using Medaka fish and may be used for the further development of biomarkers by utilizing the gene expression patterns for known contaminants.
The objective of this study is to test the applicability of the Information processing model suggested by Kaplan to the preferences of Korean people as well as to investigate the preference patterns of Korean people and the influencing factors on Korean preferences for the landscape. Photo - questionnaire survey was conducted twice to collect the data concerning the landscape preferences. The samples of the first survey were selected from the citizens of Seoul and the students of Seoul National University. The first survey focused on the preference patterns of Korean people while the emphasis of the second survey was given to the test of information processing model and the influencing factors on the preferences of Korean people. The samples of second survey were also selected from the students of Seoul National University. The photos in the photo - questionnaire for the first survey included the scenes representing both three landscape styles(Korean, Japanese and Western styles)and three landscape qualities such as the layout of space, the use of landscape plants and the use of stones and rocks. For the second survey, four informational factors such as complexity, coherence, legibility and mystery were selected for inclusion in the photos of photo - questionnaire. Respondents for both survey were asked to respond their preferences on the five - point Likers scale. The results of the study suggested that four informational factors influence significantly the preferences of Korean students. The study indicated that both the citizens of Seoul and the students of Seoul National University prefer water and vegetation to rock among the contents of landscape. Among the landscape styles, Japanese landscape style was most preferred and Western landscape style was more preferred than Korean traditional landscape style. In addition to informational factors, it was found that the contents of landscape and landscape style were also major influencing factors on the landscape preferences. The socio - economic backgrounds of respondents such as age, foreign travel experience, income, residency before the age 14, familiarity and respondent's experties in landscape architecture seemed to Influence the landscape preferences.
Objectives l: This study is to investigate the emergence of herbal medicine during Japanese colonial period by reviewing newspaper advertisements and brochures about patient medicine of Chosun Maiyak Corporation. Methods : 69 kinds of patent medications and 584 newspaper advertisements are throughly reviewed to investigate dosage form, drug effect, sales unit and method, price, and advertisement pattern. Results : Lyungsinwhan represented more than half of the total advertisements, followed by Yungmihwan, Sahyangsohabwon, Yeollyeonggobondan, and Chiljehyangbuhwan. Lyungsinwhan was advertised mostly in spring and summer whereas Yungmihwan and Yeollyeonggobondan were advertised in spring and fall. Dosage form included pill(丸), mixture(膏), powder(散), and liquid(水, 液), while pill and mixture prevailed over other forms. Drug effect included dermatological, pediatric, digestive, and gynecological effects in the increasing order of drug numbers. Sales unit and price were found to vary significantly. Sales method included nationwide network distribution of medicine through postcards and stores. Conclusion : Herbal medicine during the colonial period was distributed in various kinds of forms with different effects and advertisement patterns through diverse sales unit and price.
This paper is a study on the expression of Clothing and Textiles recorded in $\ulcorner Makuranosorsi(枕草子) \lrcorner$ This book was written by a Japanese servant in the Royal Court about the year 10000. In this book many kinds of clothing ornament color and materials were mentioned. The discoveries of this paper were as follows. 1. There were 85 different kinds of name In detail there were 46 Clothing and Accessories 32 Clors 11 Materials Patterns the method of Dyeing. 2. All the Clothing and Textiles in Makur-anosorsi were reflections of the reality of that times. 3. From ancient times japanse Costume was much influenced by Korea. but around the 7th century the influence increased. 4. Almost all clothing and Accessories used in Japan in the 10th century had been pre-viosly used in neighboring countries Korea and China But after they were imported to japan some of them were changed to japans hybrids especially in name and shape. 5. In Japan Clothing Colors were used in various ways sometimes colors coordinated by over lapping dress and othertimes the colors were weaved together Namely the width and length of these colorful fibres are also signifi-cant meaning. This meas that the Japanese was much more interested in color than other countries and this also proves Clothing Colors were very developed in japan by the 10th cen-tury. 6. The Materials discovered in this book were almost all silk This is the reason why $\ulcorner Makuranosorsi \lrcorner$ was the expression of the Royal Court.
We examined the genetic variability of Asian chum salmon (Oncorhynchus keta) populations using nuclear microsatellite (ms) DNA analysis with four polymorphic loci (OKM4, OKM5, OKM7, and OKM8) in 397 individuals from nine populations, including one in Korea, seven in Japan, and one in Russia. The msDNA gene diversity was highest in the Japanese populations, suggesting greater genetic variation in the populations in Japan than in populations in Korea and Russia. The pairwise $F_{ST}$ estimates based on our msDNA data showed that the Korean population was genetically different from the Japanese and Russian populations, and there were higher $F_{ST}$ estimates between Hokkaido and Honshu populations than between other population pairs. A neighbor-joining tree showed that the Korean population was distinct from two other clusters, representing the populations in Honshu and the populations in Hokkaido and Russia. These results suggest that the observed population genetic patterns of Asian chum salmon might be influenced by low or restricted gene flow.
This study aimed to suggest more appropriate and accurate festival costumes based on a comparative analysis on various reproduced costumes and a period review of traditional festival costumes. In particular, the comparative analysis examined festival costumes which appeared in Korean and Japanese festival parades during the Baekje Period. The Baekje Cultural and Sacheonwangsawasso Festivals were examined. A literature review as well as field research and digital restorative techniques were used in the study. The reproduction patterns of Baekje costumes from both festivals were compared with original Baekje costumes, demonstrating several differences in the configuration, material, color, and shape of the costumes in addition to the way they were worn. Based on the comparative analysis, Baekje costumes used in Festivals were grouped into three categories: King's Costume, Queen's Costume, and Government Official's Costume. Dress sketches and 3D illustrations also have been presented.
In this paper uses Kaname Akamatsu's 'Flying Geese' model to analyse Japan's role in East Asian integration. Japan made the first attempt to lead Asian countries before the Second World War. At that time, the Japanese Government embarked on a brutally expansionist policy the result of which was creation of the first gaggle of 'flying geese' under the name of the 'Greater East Asia Co-Prosperity Sphere.' During the 'flight' Japan was forcefully imposing its own ideals and values on the rest of the 'gaggle.' At the same time, the Japanese Government assumed hostile attitude toward Western countries. Japan's defeat in the Second World War signified the end of flight for the first 'flying geese' gaggle. After the war, Japan made another attempt at regional integration. This time it was done through establishing a production network in East Asia. Thus the second gaggle of 'flying geese' came into existence. During the flight of the 'second gaggle' of geese, Japan was fostering good ties with Western countries as well, especially the United States. However, some leaders of the 'second gaggle's' member-countries emboldened by their countries' economic success proclaimed that future belongs to Asia and put forward the 'Asian values' argument. The Asian economic crisis of 1997 interrupted the flight of the 'second gaggle' and effectively put an end to the 'Asian values' debate. It is interesting to note that some elements of the 'Asian values' argument resembled ultranationalist discourse that had been dominant in Japan before and during the Second World War. This paper compares historical patterns of East Asian regional integration and highlights future challenges for Japan's Asia policy.
Determining the nutrient stoichiometry in plant organs is critical for understanding nutrient uptake and cycling in forest ecosystems. This study evaluated nutrient concentrations and stoichiometry in various plant organs (stem, bark, branches, and foliage) of species found in four warm-temperate forests in southern Korea. Cryptomeria japonica D. Don (CJ), Quercus serrata Thunb. (QS), evergreen broadleaved tree species (EB), and bamboo spp. (BB) were destructively sampled to measure nutrient (C, N, and P) concentrations in the plant organs. The mean C concentration in the stem was significantly higher in CJ than in QS, BB, or EB, whereas the C concentration in the foliage was the lowest in BB. The mean foliar N and P concentrations were higher in BB than in EB or CJ. The mean stem C:N and C:P ratios were highest in CJ but were lowest in the foliage of BB. Overall, stems of all species showed a strong positive correlation between C concentration and dry weight, but a negative correlation between N and dry weight. The N and P concentrations of foliage and bark were strongly correlated, whereas those of the stem and branches were poorly correlated. Positive correlations were detected between the C:N and C:P ratios in bark and foliage. These results indicate the existence of intraspecific differences in nutrient requirements in warm-temperate forest species and add to the understanding of nutrient uptake and storage patterns in the organs of species growing in warm-temperate forests.
The goal of this study is to develop a biomarker used in monitoring abnormal behaviors of Japanese medaka (Oryzias latipes) as a model organism caused by hazardous chemicals. Japanese medaka was treated by copper of appropriate sublethal concentrations after starvation for 48 hr. The untreated individuals showed common behavioral characteristics (i.e. , smooth and linear movements). Locomotive activity of the fish was monitored using an image processing and automatic data acquisition system. When treated with copper (100 ppb), the fish showed shaking patterns more frequently. As the concentration of copper increased to 1,000 ppb, activity decreated, and the fish showed an erratic movement. Fish were exposed to copper at various concentrations (0,100 and 1,000 ppb) for 24 hrs, and acetylcholine esterase (AChE) activity was observed. When fish were exposed to 1,000 ppb of copper, the body AChE activities appeared to decrease but the head AChE activities showed little change. Expressions of tyrosine hydroxylase (TH) protein in the different organs from both head (brain) and body (kidney) portions affected by the copper treatment were analyzed using immunohistochemical technique compared with control. Five organs of the fish (olfactory bulb, hyothalamus, optic lobe, pons and myelencephalon regions) showed a relatively strong TH protein expression in the control experiment. A differential expression of TH, however, was observed in the treatment (100 ppb and 1,000 ppb). The treatment (1,000 ppb) significantly suppressed TH protein production in the brain regions. In kidney, however, the same treatment caused little suppression compared with the control. Copper appeared to be less effective in suppression of TH than diazinon, a known TH suppressor. It was concluded that TH could be used at a potential biomarker to monitor the acute copper toxicity in Japanese medaka.
Geisha's gorgeous kimonos and grotesque make-up was the object of envy to Japanese women. There is a line in the movie like "Kimonos to the Geisha is the same as colors to the painter." Likewise, splendid patterns and colorful kimonos were an indispensible method or symbol for showing their artistic accomplishments. The purpose of this study is to investigate concept and origin of Geisha and their dress and decoration, analyze the dress of the people which appear in the movie, , arrange the expression of Geisha's dress as a movie costume, compare the difference of the dress by the career of Geisha and look back the disappearing aesthetic sense of Geisha. First, since Geisha appeared for the first time in Kyoto in 1751, it has become the world-wide representative of Japanese woman's images so that its existence oneself has become the tradition. Second, Geisha created fashion of various clothes, adornments, and hair styles as a creator of the popularity. Third, the strict regulation without exception is applied in Geisha's clothing, make-up, and motion tuned Samisen, Japanese traditional strings, and there is some difference in their hair style and dress by age of Maiko. Fourth, it is famous for unique make-up that Geisha makes up their faces, necks, and shoulders white, newly paints eyes, nose, lip line like drawing a picture in a pure white paper. Geishas put a little make-up as they have a higher position. Fifth, a heroin, Chiyo, shows clothing as a maid, an apprentice Geisha-Maiko- and a formal Geisha according to time flow. The length of kimono, textures, and hair decorations are longer, more splendid, and are more various as time goes. On the other hand, her make-up is lighter.
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