• KSBB Journal
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• 제19권1호
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• pp.88-92
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• 2004

장티푸스 협막 다당체와 일본 뇌염 바이러스로 구성되어있는 혼합백신을 제조하여 마우스에서 면역성을 측정하였다. 혼합 항원 백신은 단일 항원을 투여한 경우보다 장티푸스와 일본 뇌염에 대해서 더 높은 IgG 항체 형성을 나타내었다. Aluminium hydroxide를 adjuvant로 첨가한 경우에도 첨가하지 않은 대조군에 비하며 모두 IgG 항체 형성이 증가하는 것으로 나타났다. 일본뇌염의 경우 중화항체 값 측정 시험에서도 혼합 항원백신은 일본뇌염 단독 항원 시에 비교해서 더 많은 중화항체 값을 형성하는 결과를 나타내었다. 본 실험에서 장티푸스 협막 다당체와 일본뇌염 바이러스의 혼합백신은 서로 다른 성분의 항원간에 나타날 수 있는 항체 형성에 대한 masking effect가 발생하지 않으며 오히려 synergic effect를 나타낸다는 사실을 보여주었다. 본 연구에서는 접종시기가 유사한 두 항원의 혼합 백신으로의 사용가능성을 높여주고 최근에 백신 산업에서 종류가 다른 여러 백신들을 혼합하여 다가백신을 제조 생산하는 추세의 산업화에 큰 기대효과를 제공할 수 있으리라 사려된다.

• Pediatric Infection and Vaccine
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• 제23권1호
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• pp.1-9
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• 2016

The Zika virus, a flavivirus related to dengue and Japanese encephalitis was discovered in the Zika forest in Uganda, 1947. Since Zika virus was first reported in Brazil in May 2015, infections have occurred in at least 40 countries, especially in the Americas. Zika virus infection usually is asymptomatic or causes mild illness, but may be related to severe clinical manifestations, particularly microcephaly and Guillain-$Barr{\acute{e}}$ syndrome. Although the possibility of autochthonous Zika virus transmission in South Korea is low, the imported cases and Zika virus-transmitting mosquito should be adequately monitored and promptly managed. In addition, enhancing preparedness for Zika virus infection are needed.

• Journal of Microbiology
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• 제42권1호
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• pp.25-31
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• 2004

Sf9 cells have obvious advantages for the conventional production technology of vaccine. They are useful tools for high concentration and large-scale cultures. Sf9 cells were grown to maximal concentration, 8${\times}$l0$\^$6/ cells/$m\ell$ in a 500$m\ell$ spinner flask, with a doubling time at the exponentially growing phase of 24.5 hours, using serum-free media. To explore the ability of Sf9 cells to be infected by the Japanese encephalitis (JE) virus Beijing-l strain, Sf9 cells were infected with the virus. By 4-5 days post-infection, 10-15 % of the Sf9 cells showed cytopathic effect (CPE), from granularity to the formation of syncytia and multinucleated giant cells continuously observed over a period of 35 days. Positive fluorescent reactions were detected in 30-40% of cells infected with the JE virus Beijing-l strain, and the uninfected Sf9 cells were completely negative. Virus particles, propagated in Sf9 and Vero cells, were concentrated by sedimentation on 40% trehalose cushions by ultracentrifugation, and showed identical patterns of viral morphogenesis. Complete virus particles, 40 to 50 nm in diameter, were observed, and JE virus envelope (E) proteins, at 53 kDa, were found in the western blot analysis to the anti-JE virus E protein monoclonal antibody and reacted as a magenta band in the same position to the glycoprotein staining. To evaluate whether the infectious virus was produced in Sf9 cells inoculated with the JE virus Beijing-l stain, Sf9 cells were inoculated with the virus, and sample harvested every 5 days. The titers of the JE virus Beijing-l strain rose from 1.0${\times}$l0$\^$5/ to 1.5${\times}$l0$\^$6/ pfu/$m\ell$. The infected Sf9 cells could be subcultured in serum-free medium, with no change in the plaque sizes formed by the JE virus Beijing-l strain in the plaque assay. It is suggested that the ability of the JE virus Beijing-l strain to infect Sf9 cells in serum-free media will provide a useful insect cell system, where the JE virus replication, cytopathogenicity and vaccine immunogen can be studied.

• 대한수의학회지
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• 제32권3호
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• pp.365-368
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• 1992

1988년부터 1990년 6월까지 전국의 양돈장에서 수집된 돼지 유사산 태아 74복에서 바이러스성 원인체 분리 및 혈청학적 진단을 수행하였던 바 다음과 같은 결과를 얻었다. 공시한 74복의 유사산 태아중 44복의 태아 흉강액에서 면역 globulin이 검출되어 전염성 질병감염에 의한 유사산으로 추정되었다. 이중 37%가 바이러스성 유사산으로 나타났으며 유사산의 원인체별 분포를 살펴보면 돼지 파보바이러스가 21%로 가장 높았으며, 뇌심근염 바이러스가 11%, 일본뇌염 바이러스가 9% 등의 순으로 나타났다. 한편 돼지 콜레라바이러스 및 오제스키병 바이러스에 의한 유사산이 각각 1건씩 검출되었으며 동일 유사산 태아에서 2가지 병원체가 중복감염된 예도 관찰되었다.

• 대한수의학회지
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• 제29권2호
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• pp.109-114
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• 1989

1982년(年)부터 1984년도(年度)까지 3년간(年間)에 걸처, 일본내(日本內) 중부(中部) Saitama현(縣)의 우(牛) 1,306두(頭)와 남부(南部)의 Kagoshima현(縣)의 우(牛) 536두(頭)를 대상(對象)으로 하여 일본뇌염(日本腦炎)바이라스 (JEV)의 적혈구응집억제(赤血球凝集抑制) (HI) 항체양성율(抗體陽性率)을 검사(檢査)한 바, Kagoshima현(縣)에선 68.8% 그리고 Saitama 현(縣)에서는 65.5%가 양성(陽性)이었다. 계절별(季節別)로는 양지역(兩地域)이 공(供)히 하절(夏節)에 항체양성율(抗體陽性率)이 높았고, 연령별(年齡別) 양성율(陽性率)은 Saitama 현(縣)의 경우 64.0%부터 82.8%까지 분포(分布)하고, Kagoshima현(縣)의 우(牛)는 1세군(歲群)에서 29.4%, 2세군(歲群)에선 50.0%, 3세군(歲群)에선 47.4% 그리고 4세군(歲群)에선 74.5%의 양성율(陽性率)을 나타내었다. 그리고 양지역(兩地域)의 연령(年齡)과 항체력가간(抗體力價間)에는 상관성(相關性)이 없었고, Saitama현(縣) 우(牛)의 역가(力價)는 연령(年齡)에 따라 15.3~22.5, Kagoshima 현우(縣牛)은 20.0~32.3이었다.

• 대한바이러스학회지
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• 제27권2호
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• pp.197-207
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• 1997

To investigate the NS4 region of JEV, NS4 cDNA of K94P05 (JEV strain isolated from Korea in 1994) was amplified by RT-PCR and analyzed by sequencing PCR product. Genomic size of NS4 was 1212bp and nucleotide sequence was compared with that of other JEV strains. Nucleotide homology between JaOAr582 and K94P05 was 91.1% and that between Beijing and K94P05 was 89.8%, respectively. But the nucleotide sequence of E region of JaOAr582 and K94P05 showed 97.0% homology and that of Beijing and K94P05 did 95.8% homology. NS4 protein was expressed as a form of fusion protein by a prokaryotic expression system. The induced fusion product showed a lower molecular weight than predicted size and remained insoluble. The NS4 protein might be cleavaged by E. coli protease. Concluding above results, high hydrophobicity of the NS4 protein supported the fact that this protein played a role as a membrane component and the poor nucleotide sequence conservativity among JEV strains suggested that this region might be important to adapt each viral growth environment.

• 한국임상수의학회지
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• 제30권4호
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• pp.236-240
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• 2013

A total of 170 litters (575 samples) of aborted and stillbirth fetuses submitted to the Gyeongsangbuk-Do Veterinary Service Laboratory (GVSL) between January 2006 and December 2010 from pig farms in Gyeongbuk province were studied to identify porcine abortion- and stillbirth-associated viruses such as Porcine parvovirus (PPV), Encephalomyocarditis Virus (EMCV), Japanese Encephalitis Virus (JEV), Porcine Reproductive and Respiratory Syndrome Virus (PRRSV), and Aujeszky's Disease Virus (ADV). Virus was not detected by PCR in 36 litters, but viral antibody was detected by HI and ELISA in 93 litters. The majority of etiological viruses were PPV (67 litters, 39.4%), EMCV (50 litters, 29.4%), PRRSV (15 litters, 8.8%), and JEV (11 litters, 6.5%); ADV was not detected by either PCR or ELISA. Single infection occurred in 52 litters (30.6%), co-infection occurred in 41 litters (24.1%), and unknown cases with no detection of any of the five viruses occurred in 77 litters (45.3%).

• IMMUNE NETWORK
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• 제7권2호
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• pp.66-74
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• 2007

Background: The genus Flavivirus consists of many emerging arboviruses, including Dengue virus (DV), Japanese encephalitis virus (JEV) and West Nile virus (WNV). Effective preventive vaccines remain elusive for these diseases. Mice are being increasingly used as the animal model for vaccine studies. However, the pathogenic mechanisms of these viruses are not clearly understood. Here, we investigated the interaction of DV and JEV with murine bone marrow-derived dendritic cells (bmDC). Methods: ELISA and FACS analysis were employed to investigate cytokine production and phenotypic changes of DCs obtained from bone marrow following flavivirus infection. Results: We observed that these viruses altered the cytokine profile and phenotypic markers. Although both viruses belong to the same family, JEV-infected bmDC produced anti-inflammatory cytokine (IL-10) along with pro-inflammatory cytokines, whereas DV infection induced production of large amounts of pro-inflammatory cytokines (IL-6 and TNF-${\alpha}$) and no IL-10 from murine bmDCs. Both flaviviruses also up-regulated the expression of co-stimulatory molecules such as CD40, CD80 and CD86. JEV infection led to down-regulation of MHC II expression on infected bmDCs. We also found that cytokine production induced by JEV and DV is MyD88-dependent. This dependence was complete for DV, as cytokine production was completely abolished in the absence of MyD88. With regard to JEV, the absence of MyD88 led to a partial reduction in cytokine levels. Conclusion: Here, we demonstrate that MyD88 plays an important role in the pathogenesis of flaviviruses. Our study provides insight into the pathogenesis of JEV and DV in the murine model.

• 한국동물위생학회지
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• 제41권3호
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• pp.165-169
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• 2018

This survey is performed for the vector mosquitoes including of Japanese encephalitis, Malaria and West niles virus. Black light traps were installed in 5 sites (Sejong lake, Janggun-myeon, Jeonui-myeon, Yeongi-myeon, Bukang-myeon). Mosquitoes were collected twice every week from five collection sites using a black light trap from May to September. A total of 10 species belonging to 4 genera, 14,234 female mosquitoes were collected and identified as vector mosquitoes. Among ten mosquito species, Culex pipiens complex was the most commonly mosquitoes (6,163 individuals, 43.3%). The greatest number of Culex pipiens pallens was trapped in the first week of July. A total of 115 pools from 14,234 mosquitoes were prepared according to their species, collection date and site. We have screened infection of virus in mosquitoes. All of virus were not detected in mosquitoes. Our findings confirm that population and first appearance time of vectors mosquitoes and virus infection in Sejong city.

• 한국콘텐츠학회:학술대회논문집
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• 한국콘텐츠학회 2017년도 춘계 종합학술대회 논문집
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• pp.297-298
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• 2017

플라비바이러스(Flavivirus)는 모기와 같은 곤충을 매개로 하여 인체에 감염된다고 잘 알려져 있다. 그 대표적인 예로 지카 바이러스(Zika virus), 뎅기 바이러스(Dengue virus), 황열 바이러스(Yellow fever virus), 일본 뇌염 바이러스(Japanese encephalitis virus) 등을 들 수 있다. 본 연구에서는 생물정보학을 기반으로 인체 감염 주요 플라비바이러스인 지카 바이러스, 뎅기 바이러스. 황열 바이러스, 일본 뇌염 바이러스의 총 4종류 플라비바이러스에 공통적으로 적용 가능한 펩타이드 백신 후보를 제시하고자 한다. 먼저 UniProt (The Universal Protein Resource)의 유전자 서열정보를 이용하여 4종류의 바이러스가 가진 단백질 중 백신으로써 적합한 단백질을 선정하였다. 선정된 단백질의 아미노산 서열정보를 바탕으로 IEDB (Immune Epitope Database And Analysis Resource)를 활용한 에피토프(epitope) 분석을 통해 에피토프로 작용하는 4 종류 바이러스의 공통적인 서열을 도출하였다.