• Journal of Microbiology
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• v.43 no.spc1
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• pp.93-100
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• 2005

It had long been assumed that a bacterial cell was dead when it was no longer able to grow on routine culture media. We now know that this assumption is simplistic, and that there are many situations where a cell loses culturability but remains viable and potentially able to regrow. This mini-review defines what the 'viable but nonculturable' (VBNC) state is, and illustrates the methods that can be used to show that a bacterial cell is in this physiological state. The diverse environmental factors which induce this state, and the variety of bacteria which have been shown to enter into the VBNC state, are listed. In recent years, a great amount of research has revealed what occurs in cells as they enter and exist in this state, and these studies are also detailed. The ability of cells to resuscitate from the VBNC state and return to an actively metabolizing and culturable form is described, as well as the ability of these cells to retain virulence. Finally, the question of why cells become nonculturable is addressed. It is hoped that this mini-review will encourage researchers to consider this survival state in their studies as an alternative to the conclusion that a lack of culturability indicates the cells they are examining are dead.

• Molecular & Cellular Toxicology
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• v.2 no.1
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• pp.11-14
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• 2006

In this study we attempted to develop a novel genomic method to selectively isolate target functional microbial genomes from environmental samples. For this purpose, stable isotope probing (SIP) was applied in selectively isolating organic pollutant-assimilating populations. When soil microbes were fed with $^{13}C-labeled $ biphenyl, biphenyl-utilizing cells were incorporated with the heavy carbon isotope. The heavy DNA portion was successfully separated by CsCl equilibrium density gradient. And the diversity in the heavy DNA was sufficiently reduced, being suitable for the current DNA microarray techniques to detect biphenyl-utilizing populations in the soil. In addition, we proposed a new way to get more genetic information by combining this SIP method with selective metagenomic approach. The increased selective power of these new DNA isolation methods will be expected to provide a good quality of new genetic information, which, in turn, will result in development of a variety of biomarkers that may be used in assessing ecotoxicology issues including the impacts of organic hazards, and antibiotic-resistant pathogens on human and ecological systems.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2005.11a
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• pp.92-97
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• 2005

The $9 billion US global wind energy market is experiencing dramatic growth with installed generating capacity up 500% from 7,600 MW at the end of 1997 to nearly 39,300 MW at the end of 2003. With an average annual increase approaching 32%, wind is the world's fastest growing energy source on a percentage basis, and its growth is forecast to continue a double-digit pace into the next decade 1. While much of this growth is fueled by government decisions that are favorable to 'green' or renewable Power, it is also fueled by advances in wind turbine technology as evidenced by larger, more sophisticated machines. As a result, wind turbines are becoming more established as an economically viable alternative to fossil-fueled power generation. Today, wind 'farms' - consisting of anywhere from a single turbine to as many as several hundred turbines - are an important component of the world's source of electric energy.

• Proceedings of the Korea Institute of Fire Science and Engineering Conference
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• 1997.11a
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• pp.617-624
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• 1997

Over 100 experiments have been conducted at the University of Maryland to evaluate the performance of a water mist fire suppression system for protection of an interstitial space below a raised subfloor. Experiments are conducted as part of an ongoing research effort to compare the fire suppression capabilities of various water mist system designs in a raised subfloor space. Water mist system design parameters considered in the investigation include means of actuation, concentration of water mist required for extinguishment of fires, and delivery mechanisms of water mist within close proximity to the fire. Delivery of the required concentration of water mist within close proximity to the fire is a principal factor governing the adequacy of water mist systems. Two sets of experiments have been conducted to document the performance of water mist system designs. One set is involved in documenting the concentration of water mist as a function of position within the space. The second set of experiments is concerned with the ability of water mist system designs to control fires in the space. One result of the research is the assessment of the ability of a water mist system to control fires at particular locations as a function of water mist density at that location.

• Proceedings of the Korea Institute of Fire Science and Engineering Conference
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• 1997.11a
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• pp.193-200
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• 1997

Flame spread and heat release properties and incident heat flux of interior materials subject to an igniter heat flux in a compartment are investigated and compared by using computer model. A comer fire ignition source is maintained for 10 minutes at 100 kw and subsequently increased to 300kw. In executing the model, base-line material properties are selected and one is changed for each run. Also 4 different igniter heat flux conditions and examined. Results are compared for the 12 different materials tested by the ISO Room Comer Test (9705). The time for total energy release rate to reach 1MW is examined. The parameters considered include flame heat flux and thermal inertia, lateral flame spread parameter, heat of combustion and effective heat of gasfication. The model can show the importance of each property in causing fire growth on interior Hnish materials in a compartment. The effect of ignitor heat flux and material property effects were demonstrated by using dimensionless parameters a, b and Tb. Results show that for b greater than about zero, flashover time in the ISO Room-Corner test is principally proportional to ignition time and nothing more.

• Journal of Life Science
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• v.7 no.4
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• pp.358-360
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• 1997

Soybean [Glycine max (L.) Merr.] seed size is a important yield component and is a primary consideration in the development of cultivars for specialty markets. Our objective was to examine the consistency of QTLs for seed size across generations. A 68-plant F$_{2} segregation population derived from a mating between Marcury (small seed) and PI 467.468 (large seed) was evaluated with RAPD markers. In the F$_{2} plant generation (i.e. F$_{3} seed), three markers, OPL09a, OPM)7a, and OPAC12 were significantly (P<0.01) associated with seed size QTLs. In the F$_{2} ; F$_{3} generation (i.e., F$_{4} seed), four markers, OPA092, OPG19, OPL09b, and OPP11 were significantly (P<0.01) associated with seed size QTLs. Just two markers, OPL09a, and OPL09b were significantly (P<0.05) associated with seed size QTLs in both generations. The consistency of QTLs across generations indicates that marker-assisted selection for seed size is possible in a soybean breeding program.

• Journal of Korea Multimedia Society
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• v.7 no.6
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• pp.824-831
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• 2004

This paper presents the java bytecode-to-.NET MSIL intermediate language translator which enables the execution of the java program in .NET environments without JVM(java Virtual Machine), translating bytecodes produced by compiling java programs into MSIL codes. Java, one of the most widely used programming languages recently, is the language invented by James Gosling at Sun Microsystems, which is the next generation language independent of operating systems and hardware platforms. Java source code is compiled into bytecode as intermediate code independent of each platform by compiler, and also executed by JVM. .NET language such as C# and .NET platform in Microsoft Corp. has been developed to meet the needs of programmers, and cope with Java and JVM platform of Sun Microsystems. After compiling, a program written in .NET language is converted to MSIL code, and also executed by .NET platform but not in JVM platform. For this reason, we designed and implemented the java bytecode-to-.NET MSIL translator system for programs written in java language to be executed in the. NET platform without JVM. This work improves the execution speed of programs, enhances the productivity, and provides a environment for programmers to develop application programs without limitations of programming languages.

• Steel and Composite Structures
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• v.25 no.6
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• pp.727-734
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• 2017

This paper describes a quasi-static finite element analysis, which uses the explicit integration method, of the apex joint of a cold-formed steel portal frame. Such cold-formed steel joints are semi-rigid as a result of bolt-hole elongation. Furthermore, the channel-sections that are being connected have a reduced moment capacity as a result of a bimoment. In the finite element model described, the bolt-holes and bolt shanks are all physically modelled, with contact defined between them. The force-displacement curves obtained from the quasi-static analysis are shown to be similar to those of the experimental test results, both in terms of stiffness as well as failure load. It is demonstrated that quasi-static finite element analysis can be used to predict the behavior of cold-formed steel portal frame joints and overcome convergence issues experienced in static finite element analysis.

• Steel and Composite Structures
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• v.34 no.2
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• pp.171-188
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• 2020

The past research on cold-formed steel (CFS) flexural members have proved that rectangular hollow flanged sections perform better than conventional I-sections due to their higher torsional rigidity over the later ones. However, CFS members are vulnerable to local buckling, substantially due to their thin-walled features. The use of packing, such as firmly connected timber planks, to the flanges of conventional CFS lipped I-sections can drastically improve their flexural performance as well as structural efficiency. Whilst several CFS composites have been developed so far, only limited packing materials have been tried. This paper presents a series of tests carried out on different rectangular hollow compression flanged sections with innovative packing materials. Four-point flexural tests were carried out to assess the flexural capacity, failure modes and deformed shapes of the CFS composite beam specimens. The geometric imperfections were measured and reported. The North American Specifications and Indian Standard for cold-formed steel structures were used to compare the design strengths of the experimental specimen. The test results indicate clearly that CFS rectangular 'compression' flanged composite beams perform significantly better than the conventional rectangular hollow flanged CFS sections.

• Bulletin of the Korean Chemical Society
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• v.19 no.9
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• pp.927-933
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• 1998

Polycrystalline powder of vanadium-doped forsterite (Vδ $Mg_2SiO_4$) was synthesized by the $H_2O_2$-assisted sol-gel method. The vanadium dopant, which was added as VO$(OMe)_3$ in methanol, went through several redox reactions as the sol-gel reaction proceeded. Upon adding VO$(OMe)_3$ to a mixture of $Mg(OMe)_2$ and Si$(OEt)_4$ in methanol, V(V) reduced to V(IV). As hydrolysis reaction proceeded, the V(IV) oxidized all back to V(V). Apparently, some of the V(V) reduced to V(IV) during subsequent gelation by condensation reaction. The V(IV) remained even after heat treatment of the gel in highly oxidizing atmosphere. The crystallization of the xerogel around 880 ℃ readily produced single phase forsterite without any minor phase. Using the polycrystalline powder as feeding stock, single crystals of vanadium-doped forsterite were grown by the floating zone method in oxidizing or reducing atmosphere. The doping was limited in low level because of the high partitioning of the vanadium in liquid phase during melting. The greenish single crystal absorbed visible light of 700∼1100 nm. But, no emission was obtained in near infrared range.