• Smart Structures and Systems
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• 제13권5호
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• pp.849-868
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• 2014

An indirect approach is explored for structural health bridge monitoring allowing for wide, yet cost-effective, bridge stock coverage. The detection capability of the approach is tested in a laboratory setting for three different reversible proxy types of damage scenarios: changes in the support conditions (rotational restraint), additional damping, and an added mass at the midspan. A set of frequency features is used in conjunction with a support vector machine classifier on data measured from a passing vehicle at the wheel and suspension levels, and directly from the bridge structure for comparison. For each type of damage, four levels of severity were explored. The results show that for each damage type, the classification accuracy based on data measured from the passing vehicle is, on average, as good as or better than the classification accuracy based on data measured from the bridge. Classification accuracy showed a steady trend for low (1-1.75 m/s) and high vehicle speeds (2-2.75 m/s), with a decrease of about 7% for the latter. These results show promise towards a highly mobile structural health bridge monitoring system for wide and cost-effective bridge stock coverage.

• Steel and Composite Structures
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• 제34권1호
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• pp.155-170
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• 2020

Different parameters potentially affect the properties of corroded reinforced concrete beams. However, the high number of these parameters and their dependence cause that the effectiveness of the parameters could not be simply identified. In this study, an adaptive neuro-fuzzy inference system (ANFIS) was employed to determine the most influencing parameters on the properties of the corrosion-damaged reinforced concrete beams. 207 ANFIS models were developed to analyze the collected data from 107 reinforced concrete (RC) beams. The impact of 23 input parameters on nine output factors was investigated. The results of the paper showed the order of influence of each input parameter on the outputs and revealed that the input parameters regarding the uncorroded properties of concrete beams are the most influencing factors on the corresponding corroded properties of the beams.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제16권2호
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• pp.145-151
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• 1994

앞 턱 부위가 짧고 후방으로 위치해 있을 때 턱의 높이를 증가시키고 전방으로 돌출시키는 축조 이부성형술의 방법으로는, 매식체 삽입술, 골 절단술, 골 절제술 또는 이들을 동시에 시행하는 혼합술등이 행해져 왔다. 그러나 매식체 삽입술에서는 감염 또는 매식체의 유동성 등의 합병증의 발병이 빈발하고 매식체의 밑 부위에 골이 흡수된다든지 연조직의 변화를 예측하기 어렵다든지 하는 복잡한 문제점들이 종종 보고되고 있다. 따라서 매식체를 이용하지 않는 골 절단술이나 골 절제술만을 이용한 다양한 방법의 이부성형술이 많이 시행되고 있다. 하악골이 전후방 관계로 발육부전인 환자에게 양측 하악지 시상분할술을 시행하여 전방 이동시킨 후 앞 턱의 위치를 좀더 개선하기 위하여 $Michel{\'{e}}t$ 테크닉의 변형법을 이용한 이부성형술을 추가로 시행하였다. 이 방법의 이부성형술은 턱의 수직적, 수평적 길이를 증가시키기 위하여 하악결합면 부위에 장부와 장붓구멍 모양을 한 골 절단술을 시행하고 광범위한 연조직경을 보존하여 골편으로의 혈류 차단을 최소화하였으며 나사를 이용한 견고한 고정을 용이하게 하였다. 이 술식은 턱을 수직적으로 증가시킬 때 두 골편 사이의 공간에 골 이식을 시행할 필요가 없으며, 금속판을 이용하지 않아도 정확한 위치에 골편을 고정하기가 용이하고, 또한 고정용 나사가 향후 골형성 부위에 위치하게 된다는 장점을 경험하게 되어 이에 한 증례를 보고하는 바이다.

• Bulletin of the Korean Chemical Society
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• 제33권12호
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• pp.4041-4046
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• 2012

The recombinant expression of proteins has been the method of choice to meet the demands from proteomics and structural genomics studies. Despite its successful production of many heterologous proteins, Escherichia coli failed to produce many other proteins in their native forms. This may be related to the fact that the stresses resulting from the overproduction interfere with cellular processes. To better understand the physiological change during the overproduction phase, we profiled the metabolites along the time course of the recombinant protein expression. We identified 32 metabolites collected from different time points in the protein production phase. The stress induced by protein production can be characterized by (A) the increased usage of aspartic acid, choline, glycerol, and N-acetyllysine; and (B) the accumulation of adenosine, alanine, oxidized glutathione, glycine, N-acetylputrescine, and uracil. We envision that this work can be used to create a strategy for the production of usable proteins in large quantities.

• Water Engineering Research
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• 제7권1호
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• pp.9-20
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• 2006

The watersheds are functional geographical areas that integrate a variety of environmental and ecological processes and human impacts on landscapes. Geographical assessments using GIS recognize the relationship between interdependence of resources and ecological/environmental components in watersheds. They are useful methodology for viable long term natural resource management. This paper performs through the using hydrological analyses, landscape ecological analyses, remote sensing, and GIS. Indicators are items or measures that represent key components of the small watersheds, and they are developed to be evaluated. Some indicators are described that they represent watershed condition and trend as well as focus on physical, biological and chemical properties of small watershed. Also, ecological functions such as stability, resilience, and sensitivity are inferred from them. The model implemented in GIS allows to reflect the ecological and hydrological functioning of watershed. Methodology from image analysis, landscape ecological analysis, spatial interpolation, and numerical process modeling are integrated within GIS to provide assessment for eco-logical/environmental condition. Results are described from the small watershed of Gwynns Falls in Baltimore County and Baltimore City, Maryland, an area of about 66.5 square miles. The small watershed within Gwynns Falls watershed are subject to a number of land-use. But it is predominantly urban, with significantly lesser amounts of forest and agriculture. The increasing urbanization is ass-coiated with ecological/environmental impacts and citizen conflicts.

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2010년도 학술발표회
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• pp.283-287
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• 2010

Stochastic simulation of hydrologic data has been widely developed for several decades. However, despite the several advances made in literature still a number of limitations and problems remain. In the current study, some stochastic simulation approaches tackling some of the existing problems are discussed. The presented models are based on nonparametric techniques such as block bootstrapping, and K-nearest neighbor resampling (KNNR), and kernel density estimate (KDE). Three different types of the presented stochastic simulation models are (1) Pilot Gamma Kernel estimate with KNNR (a single site case) and (2) Enhanced Nonparametric Disaggregation with Genetic Algorithm (a disaggregation case). We applied these models to one of the most challenging and critical river basins in USA, the Colorado River. These models are embedded into the hydrological software package, Pros and cons of the models compared with existing models are presented through basic statistics and drought and storage-related statistics.

• International Journal of Knowledge Content Development & Technology
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• 제10권3호
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• pp.7-20
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• 2020

This study examined the awareness and use of cloud computing services by librarians in selected universities in Edo State. A descriptive survey research design was employed and the instrument used was questionnaire. The population of the study was 132 professional and Para-professional librarians. The total enumeration technique was used to select the entire population because the size was manageable. Simple percentage, frequency count, and mean were used to analyze the data collected. From the analysis of data gathered, it was found that the librarians are aware of the use of OCLC, world cat, and Google docs to a very high extent. It was found that the librarians used cloud computing services and technologies for collection development functions and cataloging. Based on the findings it was recommended that management of the libraries should support librarians by providing adequate funding to the library in order to support the acquisition and maintenance of infrastructure for cloud computing.

• 한국미생물학회:학술대회논문집
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• 한국미생물학회 2000년도 추계학술발표대회
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• pp.15-23
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• 2000

The subcellular localization of the SopB protein, which is encoded by the Escherichia coli F plasmid and is involved in the partition of the single-copy plasmid, was directly visualized through the expression of the protein fused to the jellyfish green fluorescent protein (GFP). The fusion protein was found to localize to positions close but not at the poles of exponentially growing cells. Examination of derivatives of the fusion protein lacking various regions of SopB suggests that the signal for the cellular localization of SopB resides in a region close to its N terminus. Overexpression of SopB led to silencing of genes linked to, but well-separated from, a cluster of SopB-binding sites termed sopC. In this SopB-mediated repression of sopC-linked genes, all but the N-terminal 82 amino acids of SopB can be replaced by the DNA-binding domain of a sequence-specific DNA -binding protein, provided that the sopC locus is also replaced by the recognition sequence of the DNA-binding domain. These results suggest a mechanism of gene silencing: patches of closely packed DNA-binding protein is localized to specific cellular sites; such a patch can capture a DNA carrying the recognition site of the DNA -binding domain and sequestrate genes adjacent to the recognition site through nonspecific binding of DNA.

• Journal of Microbiology
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• 제42권2호
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• pp.69-73
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• 2004

Vibrio vulnificus, a Gram-negative bacterium found in estuarine waters, is responsible for over 95％ of all seafood-related deaths in the United States. As a result of a temperature downshift to 5$^{\circ}C$, this organism enters the viable but nonculturable (VBNC) state. Changes in the membrane fatty acid (FA) composition of V. vulnificus may be a contributing factor to the ability of this organism to enter into and survive in the VBNC state. This hypothesis was tested by incubating the organism at 5$^{\circ}C$ in arti-ficial sea water and analyzing the cells' FAs during the initial hours of temperature and nutrient down-shift. Prior to downshift, the predominant FAs were 16:0, 16:1 and 18:0. During the first four hours of downshift, statistically significant changes occurred in 15:0, 16:1, 16:0, 17:0, and 18:0. These results indicate that changes in FA composition occur prior to entry of V. vulnificus into the VBNC state, suggesting that the ability to maintain membrane fluidity may be a factor in this physiological response. Cells in which fatty acid synthesis was inhibited did not survive, indicating that active fatty acid metab-olism is essential for entry of cells into the VBNC state.

• Journal of Microbiology
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• 제42권2호
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• pp.74-79
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• 2004

Ingestion of shellfish-associated Vibrio parahaemolyticus is the primary cause of potentially severe gas-troenteritis in many countries. However, only Kanagawa phenomenon (hemolysin) positive (KP$\^$+/) strains of V. parahaemolyticus are isolated from patients, whereas >99％ of strains isolated from the environment do not produce this hemolysin (i.e. are KP$\^$-/). The reasons for these differences are not known. Following a temperature downshift, Vibrio parahaemolyticus enters the viable but noncultur-able (VBNC) state wherein cells maintain viability but cannot be cultured on routine microbiological media. We speculated that KP$\^$+/ and KP$\^$-/ strains may respond differently to the temperature and salinity conditions of seawater by entering into this state which might account for the low numbers of cul-turable KP$\^$+/ strains isolated from estuarine waters. The response of eleven KP$\^$+/ and KP$\^$-/ strains of V. parahaemolyticus following exposure to a nutrient and temperature downshift in different salinities, similar to conditions encountered in their environment, was examined. The strains included those from which the KP$\^$+/ genes had been selectively removed or added. Our results indicated that the ability to produce hemolysin did not affect entrance into the VBNC state. Further, VBNC cells of both biotypes could be restored to the culturable state following an overnight temperature upshift.