• Journal of Photoscience
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• v.9 no.2
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• pp.278-280
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• 2002

We have cloned a hydromedusan opsin cDNA and showed that the deduced amino acid sequence of the cytoplasmic loop between helices 5 and 6 (loop 5-6) was clearly different from that reported so far. The amino acid sequence of the loop 5-6 is important on determination of the specificity for the coupled G- protein. To clarify which class of G-protein mediates the phototransduction system in the ocellus of the hydromedusan, we investigated G-proteins expressed in the ocellus. By PCR against the cDNA of the ocellus with primers designed according to the conserved amino acid sequence in G-protein a subunit, we obtained three kinds of cDNA fragments. Based on the sequence similarities, ttwo of them (JGI and JG3) were classified as $G_{i}$ and $G_{q}$, respectively. The other one (JG2) was a new subtype within $G_{*}$ class. Electron microscopic immunocytochemistry with the antiserum against the C-terminal sequence of $G_{q}$ or $G_{t}$ revealed the presence. of the both classes in the ocellus. The similarity of the C-terminal sequence of the JG2 with that of bovine $G_{t}$ suggests that the anti- $G_{t}$ antiserum would bind to JG2. These results suggest the possibility that the hydromedusan rhodopsin decides the specificity for the coupled G-protein by the other domain than the loop 5-6.oop 5-6.5-6.

• The Journal of the Petrological Society of Korea
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• v.23 no.4
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• pp.337-349
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• 2014

We measured rare earth element and Zr concentrations of USGS granite standard material GSP-2 and GSJ granite standard material JG-1a to clarify the effect of zircon during rare earth element analysis using ICP-MS. We also measured rare-earth element and zirconium (Zr) contents of zircon from granite by acid-digestion methods using conventional teflon vial and pressure-bomb. The results show that acid-digestion using teflon vial dissolved ca. 50% of zircon compared to pressure-bomb method. The Zr contents of JG-1a and GSP-2 gave ca 50% of reference value. However, rare-earth element abundance of JG-1a and GSP-2 were similar to those of reference values. This suggests that the decomposition degree of zircon might give a negligible effect on a petrological and geochemical interpretation using chondritenormalized REE pattern.

• Proceedings of the PSK Conference
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• 2000.04a
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• pp.103.2-103.2
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• 2000

• Journal of the Korean Society of Food Culture
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• v.35 no.2
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• pp.215-223
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• 2020

Ganjang and doenjang are known as major fermented soy-based foods in Koreans. Current investigations have proved that fermented soybean foods impart anti-cancer, anti-obesity, and anti-diabetic effects. The aim of this study was to evaluate the anti-cancer activities of commercialized soy food, Ganjang, as a function of aging period. The test groups were classified into four time periods-short (under 5 years, S group), mid (under 10 years, M group), long (under 15 years, L group), and eternal (over 15 years, E group). The anti-cancer effects of Ganjang were determined by cell cytotoxicity assay of three types of cancer cell lines and splenocyte proliferation assay. Besides these assays, we also analyzed NK cell activity for cancer immunotherapy. The results show that the anti-cancer effect increased in the S and M period aging groups for all three cancer cell lines. Interestingly, similar to the anti-cancer result, splenocyte proliferation and NK activity showed the highest effect in the S and M groups. In contrast, Japanese ganjang-treated (JG1, JG2) groups and E group showed significantly reduced splenocyte proliferation. Collectively, these results suggest that the short and middle periods of traditional fermented Ganjang might have potential anti-cancer activities.

• Korean Journal of Acupuncture
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• v.19 no.2
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• pp.1-12
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• 2002

Objectives : Assuming That the Characteristic of Meridian System Has Been Similar to This of Electric Potentials in Human Body and That Measurements of Electric Potential at Well and Sea Points in Branches of the Twelve Meridians Will Be Representative of Measurements of the Twelve Meridians, to Measure the Electric Potentials in Healthy Volunteers(HG), Patients Diagnosed As a Cerebral Infarction(CG), a Arrhythmia(AG), a Other Intervertebral Disc Disorder(IG), and a Joint Disorder(JG), and Then to Find Out the Characteristic of Biophysical Meridian System, Finally to Compare with the Results of the Electric Potential in Those Groups. Methods : We Selected Who HG Were Diagnosed by a Blood Test, Urine Examination and Differentiation of Syndromes by Five Viscera among Volunteers, CG by CT and Wind-Syndrome Caused by Hyperactivity of the Liver-Yang, AG by EKG, CT, and Deficiency or the Heart Blood, IG by X-ray or CT and Pain in Waist, and JG by X-ray and Knee Pain. Their Electric Potential of Well and Sea Points in the Meridians Were Measured by hysiograph. Results and Conclusions : Measurements Were Analyzed by Factor Analysis, Analysis of Variance, and Logistic Regression Model, We Obtained That Most of the Results, In Conclusion, Hold Good for the Classical Meridian Doctrine.

• Proceedings of the Microbiological Society of Korea Conference
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• 1997.04a
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• pp.67.2-67.2
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• 1997

• Journal of the Korean Electrochemical Society
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• v.22 no.4
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• pp.172-176
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• 2019

The first commercialized cathode material, $LiCoO_2$, suffers from disadvantages such as high cost and toxicity and also possesses safety problems. The nickel-rich $LiNi_{0.9}Mn_{0.1}O_2$ cathode material, used as an alternative to $LiCoO_2$, has highly reversible capacity and high energy density. So, the nickel-rich $LiNi_{0.9}Mn_{0.1}O_2$ cathode material is widely used as an alternative to $LiCoO_2$ due to its highly reversible capacity and high energy density. However, $LiNi_{0.9}Mn_{0.1}O_2$ has several disadvantages as well, such as poor cycle performance and poor thermal instability. To address these problems, we synthesized a new material, $LiNi_{0.5}Mn_{0.5}O_2$, as a shell on the surface of a core to suppress the surface degradation. The new material showed high structural and thermal stabilities and could also maintain a high capacity. The capacity retention of the core-shell cathode (87.7%) was better than that of the core cathode (76.9%) after 50 cycles. Analysis using differential scanning calorimetry revealed that the heat generation in the core-shell cathode ($65.9Jg^{-1}$) was lower than that in the core cathode ($559.7Jg^{-1}$).

• Analytical Science and Technology
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• v.33 no.6
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• pp.245-251
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• 2020

Lead (Pb) has been shown to be a useful tracer of contamination sources and geochemical processes such as age dating and crustal evolution. These studies require a chromatographic technique for Pb separation from geological samples. This paper presents a comparison study on the effect of eluent concentration between 6M HCl and 8M HCl on the separation of Pb from Pb resin. The results showed that the separation of Pb using 6M HCl as the eluent was not effective compared to the separation using 8M HCl. To verify this method, we measured the Pb isotopic compositions of the Pb isotopic standard (NIST NBS981) and geological reference materials (BCR-2, GSP-2, and JG-1a) using a thermal ionization mass spectrometer (TIMS). The results correspond well with the reported values within the error range, implying that this method can be useful.

• The Journal of Natural Sciences
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• v.14 no.2
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• pp.83-91
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• 2004

Recently phage K11 lysozyme was cloned and characterized in our lab. The K11 lysozyme was identified to have dual functions. It not only cuts a peptidoglycan bond in bacterial cell wall but also acts as an inhibitor of K11 RNA polymerase. It has been known that the T7 lysozyme binds specifically to T7 RNA polymerase and inhibits transcription. The dual activities of K11 lysozyme are atreeable to the case of T7 phage lysozyme and RNA polymerare. In order to identify the binding magnitude of K11 lysozyme with K11 RNA polymerase, yeast two-hybrid system was used. K11 phage lysozyme gene was introduced into pLexA plasmid and used as a prey. Also, K11 phage RNA polymerase gene was introduced into pJG4-5 and used as a bait. The binding between K11 lysozyme and K11 RNA polymerase was demonstrated by expression of reporter genes such as lacZ and leu2.

• Journal of Plant Biotechnology
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• v.49 no.1
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• pp.61-73
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• 2022

In this study, we developed a reliable and efficient Agrobacterium-mediated genetic transformation system by applying sonication and vacuum infiltration to six chickpea cultivars (ICCV2, ICCV10, ICCV92944, ICCV37, JAKI9218, and JG11) using embryo axis explants. Wounded explants were precultured for 3 days in shoot induction medium (SIM) before sonication and vacuum infiltration with an Agrobacterium suspension and co-cultivated for 3 days in co-cultivation medium containing 100 µM/l of acetosyringone and 200 mg/l of L-cysteine. Responsive explants with putatively transformed shoots were selected using a gradual increase in kanamycin from 25 mg/l to 100 mg/l in selection medium to eliminate escapes. Results showed optimal transformation efficiency at a bacterial density of 1.0, an optical density at 600 nm wavelength (OD₆₀₀), and an infection duration of 30 min. The presence and stable integration of the β-glucuronidase (gusA) gene into the chickpea genome were confirmed using GUS histochemical assay and polymerase chain reaction. A high transformation efficiency was achieved among the different factors tested using embryo axis explants of cv. JAKI 9218. Of the six chickpea cultivars tested, JAKI9218 showed the highest transformation efficiency of 8.6%, followed by JG11 (7.2%), ICCV92944 (6.8%), ICCV37 (5.4%), ICCV2 (4.8%), and ICCV10 (4.6%). These findings showed that the Agrobacterium-mediated genetic transformation system will help transfer novel candidate genes into chickpea.