• Title/Summary/Keyword: JEJU-HORSE

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The Differences in Chemical Composition, Physical Quality Traits and Nutritional Values of Horse Meat as Affected by Various Retail Cut Types

  • Seong, Pil Nam;Park, Kyoung Mi;Kang, Geun Ho;Cho, Soo Hyun;Park, Beom Young;Chae, Hyun Seok;Ba, Hoa Van
    • Asian-Australasian Journal of Animal Sciences
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    • v.29 no.1
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    • pp.89-99
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    • 2016
  • The effects of retail cut type on chemical, quality and nutritional characteristics of horse meat were studied. Jeju female breed horses (n = 9) at 32-mo-old were slaughtered and the carcasses at 24 h post-mortem were fabricated into 10 retail cuts including: tender-loin, loin, strip-loin, shoulder-chuck-roll, shoulder-clod, top-round, outside-round, brisket, short-plate-brisket, and shank. The results revealed that all of parameters (chemical, meat quality and nutritional composition) examined significantly (p<0.05) differed between the cuts. The chemical composition range (minimum to maximum) of cuts was found as such: moisture 65.06% to 71.69%; protein 19.07% to 21.28%; collagen 1.40% to 2.45%; fat 2.56% to 12.14% and cholesterol 55.76 to 79.50 mg/100 g. Shoulder-chuck-roll had the highest pH and water-holding capacity, while top-round had the highest cooking loss. Shear force ranged between the cuts from $2.80kg/cm^2$ to $4.98kg/cm^2$. The Cu, Fe, and Zn contents ranged between the cuts from 1.52 mg/kg to 2.75 mg/kg, 21.25 mg/kg to 30.85 mg/kg, and 16.51 mg/kg to 40.42 mg/kg, respectively. Additionally, most of the cuts studied showed favorable polyunsaturated fatty acid/saturated fatty acid, n-3/n-6 and essential amino acid/non-essential amino acid ratios.

Establishing of Optimal Culture Conditions for MIC Panels for MIC Determination of Fish Bacterial Pathogens (어류 병원성 세균의 MIC 결정을 위한 MIC Panel의 최적화 배양 조건 확립)

  • Kim, Ye Ji;Jun, Lyu Jin;Kang, Mi Rae;Lee, Da Won;Woo, Soo Ji;Kim, Myoung Sug;Jeong, Joon Bum
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.53 no.3
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    • pp.443-450
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    • 2020
  • No established method can be used to select effective antibiotics in antibiotic susceptibility tests for fish bacterial pathogens quickly and accurately. Here, we established the optimal conditions for determining the minimal inhibitory concentration (MIC) of major fish bacterial pathogens (Streptococcus spp., Edwardsiella tarda, Vibrio spp., Aeromonas spp., and Pseudomonas spp.) using the KRAQ1 and CAMPY2 panels. The MIC panel used 18 antibiotics of two types and we conducted experiments to establish the optimal culture medium and temperature for each species. The optimal conditions for incubating Streptococcus spp. were in cation-adjusted Mueller-Hinton broth with TES buffer (CAMHBT) at 28℃, using 5% lysed horse blood (LHB) as recommended by the Clinical Laboratory Standards Institute. For Vibrio spp., the optimal culture conditions were 28℃ in CAMHBT supplemented with 1% NaCl. The optimal conditions for culturing E. tarda, Aeromonas spp., and Pseudomonas spp. were in CAMHBT at 28℃.

Estimation of genetic parameters for temperament in Jeju crossbred horses

  • Kim, Nam Young;Son, Jun Kyu;Cho, In Cheol;Shin, Sang Min;Park, Seol Hwa;Seong, Pil Nam;Woo, Jae Hoon;Park, Nam Geon;Park, Hee Bok
    • Asian-Australasian Journal of Animal Sciences
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    • v.31 no.8
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    • pp.1098-1102
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    • 2018
  • Objective: Temperament can be defined as a type of behavioral tendency that appears in a relatively stable manner in responses to various external stimuli over time. The aim of this study was to estimate genetic parameters for the records of temperament testing that are used to improve the temperament of Jeju crossbred (Jeju${\times}$Thoroughbred) horses. Methods: This study was conducted using 205 horses (101 females and 104 males) produced between 2010 and 2015. The experimental animals were imprinted and tamed according to the Manual for Horse Taming and Evaluation for Therapeutic Riding Horses and evaluated according to the categories for temperament testing (gentleness, patience, aggressiveness, sensitivity, and friendliness) between 15 months and 18 months of age. Each category was scored on a five-point linear scale. Genetic parameters for the test categories were analyzed using a multi-trait mixed model with repeated records. The ASReml program was used to analyze the data. Results: The heritability of gentleness, patience, aggressiveness, sensitivity and friendliness ranged from 0.08 to 0.53. The standard errors of estimated heritability ranged from 0.13 to 0.17. The test categories showed high genetic correlations with each other, ranging from 0.96 to 0.99 and high repeatability, ranging from 0.70 to 0.73. Conclusion: The results of this study showed that the test categories had moderate heritability and high genetic correlations, but additional studies may be necessary to use the results for the improvement programs of the temperament of Jeju crossbred horses.

Successful Treatment of Quarter Crack Using Wiring and Polymethylmethacrylate Composites in a Thoroughbred Racehorse (경주마에서 와이어와 폴리메틸메타크릴레이트 합성충진제를 사용한 제측열제의 치료 1례)

  • Park, Kyung-won;Ahn, Seoung-jue;Lee, Eun-bee;Chun, Yong-woo;Jeong, Hyohoon;Kang, Tae-Young;Seo, Jong-pil
    • Journal of Veterinary Clinics
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    • v.37 no.5
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    • pp.278-281
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    • 2020
  • A 4-year-old Thoroughbred stallion was referred to J&C Equine Hospital with a quarter crack of the right front foot. On visual inspection, there was a medial quarter crack with laminar necrosis. The first step to treat this problem was to remove the necrotized tissue around the crack using Dremel tools, disinfecting crack, administering antibiotics through regional limb perfusion and systemically administering antibiotics and anti-inflammatory drugs consistently. After one month's anti-inflammatory management, the quarter crack was treated successfully using two series of wiring with one week's term and polymethylmethacrylate composites (Equilox). After treatment, this racehorse successfully resumed training and racing in Korea Racing Authority Seoul Race Course, and won three times, finishing second and third place once, out of thirteen starts.

Analysis of the Coordination of the Trunk Tilting Angle and Bilateral Lower Limbs According to the Stirrups Length during Trot in Equestrian: Asymmetric Index Development of Overall Movement Index Algorithm (승마 속보 시 등자 길이에 따른 체간기울기와 양측 하지의 협응성 비교분석 : 비대칭 지수 및 전체이동지수 알고리즘 개발)

  • Hyun, Seung-Hyun;Ryew, Che-Cheong
    • Korean Journal of Applied Biomechanics
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    • v.25 no.1
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    • pp.131-140
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    • 2015
  • Purpose : The purpose of this study was to analyze the coordination of the trunk tilting angle and bilateral lower limbs according to the stirrups length during trot in equestrian. Methods : Participants selected as subject were consisted of adult male(n=7, mean age: $45.00{\pm}3.78yrs$, mean height: $172.50{\pm}2.44cm$, mean body mass: $76.95{\pm}4.40kg$, mean, mean leg length: $97.30{\pm}2.60cm$). They were divided into 3-types of stirrups lengths(67 cm, 72 cm, 77 cm) during trot. The variables analyzed were consisted of the trunk front-rear angle, lower limb joint(Right Left hip, knee, ankle), overall movement index(OMI) of the lower limbs(thigh, shank, foot) and asymmetry index(AI%) during trot. Results : The average angle in hip and knee joint showed more extended posture according to the increase of stirrups lengths and ankle angle showed more plantarflexion posture according to increase of stirrups length during 1 stride in trot. Also, average angle showed more extended posture in right hip and ankle joint than that of left. The angle of knee joint didn't show significant difference statistically between right and left. Also asymmetric index in average angle of hip, knee and ankle joint didn't show significant difference statistically in between lower limbs, but hip joint showed higher asymmetric index in stirrup length of 77 cm and ankle joint showed higher asymmetric index in stirrup length of 67 cm than that of the others respectively. The FR angle in trunk of horse-rider showed relative backward leaning motions at stirrup length of 67 cm and 77 cm than that of stirrup length of 72 cm during stance and swing phase. OMI in thigh, shank, and foot limbs didn't show significant difference statistically according to the stirrups length of right and left lower limbs, but left lower limbs showed higher index than that of right lower limb. Stirrup length of 72 cm in shank and foot limbs showed higher index than that of stirrup length of 67 cm and 77 cm. But stirrup length of 72 cm showed higher asymmetric index than that of stirrups length of 67 cm and 77 cm. Conclusions : When considering the above, 72 cm(ratio of lower limb 74.04%) stirrup lengths could be useful in posture correction and stabilization than 67cm(ratio of lower limb 68.69%) and 77 cm(ratio of lower limb 79.18%) stirrup lengths during trot in horse back riding.

Genetic Characterization of Wolla Coat Color in Jeju Horses (제주마에서 월라 모색의 유전적 특성)

  • Kim, Nam-Young;Shin, Kwang-Ynu;Lee, Chong-Eon;Han, Sang-Hyun;Lee, Sung-Soo;Park, Yong-Sang;Ko, Moon-Suck;Hong, Hyun-Ju;Yang, Jae-Hyuk;Jang, Deok-Jee;Yang, Young-Hoon
    • Journal of Animal Science and Technology
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    • v.54 no.5
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    • pp.375-379
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    • 2012
  • This study was carried out to define the "Wolla" coat color using 376 Jeju registered horses (white patched 142, solid coat color 234). Three major factors related to the white patches i.e ECA3-inversion for Tobiano, EDNRB 2 bp nucleotide substitution for frame Overo, and the KIT intron 16 single nucleotide polymorphism (SNP) for Sabino types of coat color were analyzed. It was found that out of 142 Jeju horses with white patches that have the genotype for ECA3-inversion (To) 140 horses were +/To heterozygous and 2 horses were To/To homozygous all Jeju horses with white patches had ECA3-inversion allele. However, there was no frame Overo or Sabino allele type in EDNRB and KIT intron 16 SNP in Jeju horses with white patches. As for 234 Jeju horses with a solid coat color, there was no ECA3-inversion allele related to the white patches. Thus, it could be considered that Wolla coat color with white patches in Jeju horses might have come from the Tobiano line in the genetic classification by coat color.

Ossifying Epulis in a Thoroughbred Foal (더러브렛 망아지에서 발생한 골화성 치은종)

  • Kim, Joon-Gyu;Mun, Seong-Hwan;Ko, Kyu-Ryeon;Kim, Jae-Hoon
    • Journal of Veterinary Clinics
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    • v.31 no.2
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    • pp.159-162
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    • 2014
  • A 4-month-old female thoroughbred foal was presented with large mass on mandibular gingiva. The enlarged mass was firm and yellowish pink in color, and occupied the whole mandibular arcade. The filly could not close her mouth with her lips. Radiographs showed radioopaque mass under the mandible. The mass was surgically excised from mandible. Histopathologically, gingival mass was characterized by hyperplastic gingival epithelium, well vascularized collagenous stroma and large area of bony tissues. Based on the gross and histopathologic findings, this case was diagnosed as ossifying epulis in the mandibular gingiva. This is the first report of ossifying epulis in a thoroughbred horse in Korea.

Measurement of Size of Ovaries, Follicles, and Corpus Lutea by Ultrasonography with Jeju Horse (초음파상을 이용한 제주마의 난소, 난포 및 황체의 크기 측정)

  • Yoo, J.G.;Kang, M.S.;Son, W.J.;Yun, Y.M.;Lee, J.M.;Kang, T.Y.
    • Journal of Embryo Transfer
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    • v.22 no.3
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    • pp.191-194
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    • 2007
  • The purpose of the present study was to set up basic information of size and status of ovaries by using ultrasonography to retrieve in vivo matured oocytes with ovum pick- up method. Ovaries were collected from the abattoir in Jeju in May and June which is breeding season. When the size of ovaries on ultrasonography was compared with real size measured by caliper, no significant difference was shown (p<0.05). The number of preovulatory follicles (>21mm) was investigated with ultrasonography and naked eyes. Ultrasonography group had 0.83 preovulatory follicles per ovary and naked-eye group had 0.75 preovulatory follicle per ovary and their average size was 2.86 cm and 2.34 cm, respectively. The average number of follicle was 4.25 with ultrasonography and 4.38 with naked eyes. There was no significant difference considering the size of follicle and number of follicle between ultrasonography and actual size except for the size of preovulatory follicle, suggesting that information of ultrasonography is able to use for OPU or other reproductive technology of mare.

Changes in the Productivity and the Percentage of Grasses Intake in Different Mixtures Grazed by Thoroughbred Horses (제주지역 말 방목 초지의 혼파조합별 생산성 및 채식율 변화)

  • Lee, Chong-Eon;Park, Nam-Geon;Park, Hyung-Soo;Oh, Woon-Yong;Ko, Moon-Suck;Kim, Dong-Hun;Kang, Dong-Hee
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.27 no.2
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    • pp.123-128
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    • 2007
  • A study was conducted over two years to determine the temperate mixtures of grasses for grazing horses in Jeju, Korea. The experiment field located at 450 m altitude (Jeju Horse Training Center of Korea Racing Association) was divided into 3 different mixtures plots (3 replications) without a fence between treatments, and sown by three main grasses (orchardgrass, perennial ryegrass and timothy). Seven castrated adult horses were kept on pasture from the middle of April to the end of November without supplementary concentrates. The dry matter yield, percentage of grasses intake and botanical composition were determined during the grazing period. Nutrients in grasses and soils were analyzed. The dry matter yield during two-year experimental period was not different among mixtures (11,000-12,000 kg/ha/year). In botanical composition, perennial ryegrass was dominant in all mixtures during spring through early summer season, but faded away during hot summer season. Timothy seemed to be more resistant against a hot or dry weather than other grasses. The percentage of grasses intakes was higher in the mixture of timothy than those in other mixtures. The contents of NDF (neutral detergent fiber) and ADF (acid detergent fiber) were not different among different mixtures. These results indicate that the mixture of timothy, orchardgrass, perennial ryegrass and white clover (20, 5, 5, 2 kg/ha as a seeding rate) can be used for gazing pasture of horses in the high altitude of Jeju.

PCR-RFLP for the Identification of Mammalian Livestock Animal Species

  • Han, Sang-Hyun;Park, Seon-Mi;Oh, Hong-Shik;Kang, Geunho;Park, Beom-Young;Ko, Moon-Suck;Cho, Sang-Rae;Kang, Yong-Jun;Kim, Sang-Geum;Cho, In-Cheol
    • Journal of Embryo Transfer
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    • v.28 no.4
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    • pp.355-360
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    • 2013
  • Precise, rapid and simple methods for species identification in animals are among the most important techniques in the livestock industry and research fields including meat classification. In this study, polymerase chain reaction (PCR) based molecular identification using inter species polymorphisms were examined by PCR-restriction fragment length polymorphism (RFLP) analysis for mitochondrial DNA (mtDNA) cytochrome b (CYTB) gene sequences among four mammalian livestock animals (cattle, horse, goat and pig). The results from PCR-RFLP analysis using the AluI restriction enzyme were also provided for the species-specific band patterns among CYTB gene sequences in these four species. The AluI-digestion for CYTB genes provided interesting migration patterns differentially displayed according to each species. Cattle and horse had one AluI-recognition site at different nucleotide positions and their AluI-digested fragments showed different band patterns on the gels. Pig had two AluI-recognition sites within the amplified CYTB sequences and produced three bands on the gels. Goat had no AluI-recognition site and was located at the same position as the uncut PCR product. The results showed the species-specific band patterns on a single gel among the four livestock animal species by AluI-RFLP. In addition, the results from blind tests for the meat samples collected from providers without any records showed the identical information on the species recorded by observing their phenotypes before slaughter. The application of this PCR-RFLP method can be useful and provide rapid, simple, and clear information regarding species identification for various tissue samples originating from tested livestock species.