초음파상을 이용한 제주마의 난소, 난포 및 황체의 크기 측정

Measurement of Size of Ovaries, Follicles, and Corpus Lutea by Ultrasonography with Jeju Horse

  • Yoo, J.G. (Department of Veterinary Medicine, Cheju National University) ;
  • Kang, M.S. (Majour of Animal Science and Biotechnology, Cheju National University) ;
  • Son, W.J. (Department of Veterinary Medicine, Cheju National University) ;
  • Yun, Y.M. (Department of Veterinary Medicine, Cheju National University) ;
  • Lee, J.M. (Department of Veterinary Medicine, Cheju National University) ;
  • Kang, T.Y. (Department of Veterinary Medicine, Cheju National University)
  • 발행 : 2007.09.29

초록

본 연구는 도축된 제주마의 난소를 이용하여 초음파 유도난포란 채취 기술을 확립하고자 난소, 난포 및 황체의 크기를 초음파상과 육안적 측정치를 비교하고자 하였다. 초음파상 측정치와 caliper를 이용한 실제 크기의 측정치 비교에서는 통계학적 차이는 보이지 않았다. 배란 직전의 난포를 조사한 결과, 초음파상에서 관찰한 것은 난소 한 개 당 평균 0.83개와 평균 크기는 2.86 m였으며, 육안으로 관찰된 것은 난소 한 개 당 평균 0.75개와 평균 크기는 2.3 cm였다. 난소 한 개 당 평균난포 수는 초음파상에서는 4.25개와 육안에서는 4.38개였다. 제주마 난소의 난포 크기 및 수를 초음파상과 육안으로 조사하였던 바, 유의적인 차이가 나타나지 않았다. 이러한 결과로 차후 초음파를 이용한 OPU와 말에서 번식 기술과 관련된 연구에 기초 정보가 제공되리라 사려된다.

The purpose of the present study was to set up basic information of size and status of ovaries by using ultrasonography to retrieve in vivo matured oocytes with ovum pick- up method. Ovaries were collected from the abattoir in Jeju in May and June which is breeding season. When the size of ovaries on ultrasonography was compared with real size measured by caliper, no significant difference was shown (p<0.05). The number of preovulatory follicles (>21mm) was investigated with ultrasonography and naked eyes. Ultrasonography group had 0.83 preovulatory follicles per ovary and naked-eye group had 0.75 preovulatory follicle per ovary and their average size was 2.86 cm and 2.34 cm, respectively. The average number of follicle was 4.25 with ultrasonography and 4.38 with naked eyes. There was no significant difference considering the size of follicle and number of follicle between ultrasonography and actual size except for the size of preovulatory follicle, suggesting that information of ultrasonography is able to use for OPU or other reproductive technology of mare.

키워드

참고문헌

  1. Bracher V, Parlevliet JM, Pieterse MC, Vos PL, Wiemer P, Taverne MA and Colenbrander B. 1993. Transvaginal ultrasound-guided twin reduction in the mare. Vet. Rec., 133: 478-479 https://doi.org/10.1136/vr.133.19.478
  2. Bruck I, Synnestvedt B and Greve T. 1997. Repeated transvaginal oocyte aspiration in unstimulated and FSH-treated mares. Theriogenology, 47:1157-1167 https://doi.org/10.1016/S0093-691X(97)00096-4
  3. Carnevale EM and Ginther OJ. 1992. Relationships of age to uterine function and reproductive efficiency in mares. Theriogenology, 37:1101-1115 https://doi.org/10.1016/0093-691X(92)90108-4
  4. Carnevale, EM and Ginther OJ. 1993. Use of a linear ultrasonic transducer for the transvaginal aspiration and transfer of oocytes in the mare. J. Equine Vet. Sci., 13:331-333 https://doi.org/10.1016/S0737-0806(06)81118-4
  5. Hinrichs K and Kenney RM. 1987. Effect of timing of progesterone administration on pregnancy rate after embryo transfer in ovariectomized mares. J. Reprod. Fertil. Suppl., 35: 439-443
  6. Hinrichs K, Matthews GL, Freeman DA and Torello EM. 1998. Oocyte transfer in mares. J. Am. Vet. Med. Assoc., 212: 982-986
  7. Hinrichs K, Provost PJ and Torello EM. 1999. Birth of a foal after oocyte transfer to a nonovulating, hormone-treated recipient mare. Theriogenology, 51:1251-1258 https://doi.org/10.1016/S0093-691X(99)00069-2
  8. Hinrichs K. 1991. The relationship of follicle atresia to follicle size, oocyte recovery rate on aspiration, and oocyte morphology in the mare. Theriogenology, 36:157-168 https://doi.org/10.1016/0093-691X(91)90375-N
  9. Hinrichs K. 1998. Production of embryos by assisted reproduction in the horse. Theriogenology, 49: 13-21 https://doi.org/10.1016/S0093-691X(97)00398-1
  10. McKinnon AO, Squires EL, Harrison LA, Blach EL and Shideler RK. 1988. Ultrasonographic studies on the reproductive tract of mares after parturition: effect of involution and uterine fluid on pregnancy rates in mares with normal and delayed first postpartum ovulatory cycles. J. Am. Vet. Med. Assoc., 192:350-353
  11. Palmer E, Bezard J, Magistrini M and Duchamp G. 1991. In vitro fertilisation in the horse. A retrospective study. J. Reprod. Fertil. Suppl., 44:375-384
  12. Pierson RA, Kastelic JP and Ginther OJ. 1988. Basic principles and techniques for transrectal ultrasonography in cattle and horses. Theriogenology, 29:3-20
  13. Pieterse MC, Kappen KA, Kruip TA and Taverne MA. 1988. Aspiration of bovine oocytes during transvaginal ultrasound scanning of the ovaries. Theriogenology, 30:751-762 https://doi.org/10.1016/0093-691X(88)90310-X
  14. Sertich PL. 1989. Transcervical embryo transfer in performance mares. J. Am. Vet. Med. Assoc., 195:940-944
  15. Squires EL, McCue PM and Vanderwall D. 1999. The current status of equine embryo transfer. Theriogenology, 51: 91-104 https://doi.org/10.1016/S0093-691X(98)00234-9
  16. Squires EL. 1996. Maturation and fertilization of equine oocytes. Vet. Clin. North. Am. Equine Pract., 12:31-45 https://doi.org/10.1016/S0749-0739(17)30293-6
  17. Van der Shans A, van der Westerlaken LAJ, de Witt AAC, Eyestone WH and De Boer HA. 1991. Ultrasound-guided transvaginal collection of oocytes in the cow. Theriogenology, 35:288 https://doi.org/10.1016/0093-691X(91)90264-E
  18. Vogelsang MM, Kraemer DC, Potter GD and Stott GG. 1987. Fine structure of the follicular oocyte of the horse. J. Reprod. Fertil. Suppl., 35:157-167