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Studies on Genetic Toxicity of Epoxidized Soy Bean Oil (에폭시화 대두유의 유전독성 연구)

  • 한의식;정해관;김종원;박미선;엄미옥;강혁준;민수진;오혜영
    • Journal of Food Hygiene and Safety
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    • v.16 no.2
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    • pp.145-151
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    • 2001
  • EpoxidiBed soy bean oil (ESBO) is a plasticizer of PVC which is being widely used as a gaskets for the lid of glass jars including baby food. Using reverse mutation assay, chromosome aberration test and micronucleus test, ESBO were evaluated the mutagenicity. In the reverse mutation test, ESBO did not induced mutagenicity in Salmonella typhimurium TA98, TA100, TA1535, TA1537, TA102 with and without metabolic activation. In the chromosome aberration test using CHL cells, the results showed no increased structural and numerical aberrations in the concentration of sample producing cytotoxicity with and without metabolic activation. The in vivo induction of micronuclei was measured in polychromatic erythrocytes of bone marrow of young (3weeks old) and adult (6 weeks old) ddY mice of both sex. At 24 hours after treatment with ESBO 20, 10, 5, 2.5 g/B.W. kg/corn oil 10 ml by oral route animals were sacrificed and bone marrow cells were prepared for smear slides. The results showed no increased micronucleated polychromatic erythrocytes regardless of sex and age. It was concluded that water soluble ESBO did not show certain genotoxicity within our studies conducted.

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Traditional Jeupjang - A Study on Traditional Jeupjang (Succulent Jang) - (전통즙장 - 전통 즙장에 대한 연구 -)

  • Ann, Yong-Geun;Moon, Young-Ja
    • The Korean Journal of Food And Nutrition
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    • v.28 no.5
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    • pp.835-848
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    • 2015
  • In the past, Korea had many kinds of jeupjang (succulent jang), a rapidly maturing original Korean jang (fermented soybean paste) of which there is no record in Chinese cookbooks. However, this local delicacy has almost been forgotten. Therefore, we looked for information about jeupjang in cookbooks written prior to the Joseon Dynasty in Korea (1392~1910) and in the 1950s. Among the recipes, there were 34 jeupjangs prepared with vegetables, such as eggplant and cucumber, and 9 without. The main ingredients of jeupjang are soybean, bran (wheat crust), and barley, and wild wheat is also used. Jeupjang is made in small portions to expedite its rapid maturation, but the most common form is egg-shaped, and there is also a flat or round, hilt-shaped version. In most cases, jeupjang consists of a mixture of meju powder (moldy soybean), water, and salt. Other ingredients can include nuruk (moldy bran), bran, wheat flour, an alcoholic beverage, maljang (dried fermented soybeans), ganjang (liquid soy sauce), malt, and takju (Korean murky wine). Jeupjang meju can be fermented in a vessel, most widely in baskets made of straw (sum and dungumi) or willow or interwoven twigs (chirung), but jars can also be used. The leaves of the paper mulberry are generally used for the mat and cover, but straw or leaves of the sumac, mulberry, or pine tree, soy, and fallen leaves are also used. Unlike other jangs, jeupjang is matured at $60^{\circ}C$ to $65^{\circ}C$, using heat emitted from the decomposition of horse dung, haystacks, or manure. Jeupjang became defunct or was transformed into jeomjang, jiraejang, mujang, paggeumjang, makjang, jipjang, and tojang. These jangs differ from jeupjang in that they use rice, malt, or hot pepper powder.

Effect of packaging conditions on the quality changes of fermented soy paste and red pepper paste (포장조건에 따른 한국전통 된장과 고추장의 품질변화)

  • Jang, Jae-Deck;Hwang, Yong-Il;Lee, Dong-Sun
    • KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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    • v.6 no.1
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    • pp.31-36
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    • 2000
  • 180g of fermented soy paste and 150g of red pepper paste were packaged in glass jars of 232 mL with different conditions of active packaging and then stored at $13^{\circ}C$ for about 170 and 128 days, respectively. During the storage, package atmosphere, surface color, pH, acidity and microbial flora were monitored to see the effect of packaging conditions. Test packaging conditions include package equipped with $Ca(OH)_2\;as\;CO_2$ absorber, package with pinhole and closed control one. Closed control packages of soypaste and red pepper paste showed the increased $CO_2$ partial pressure, the decreased $O_2$ partial pressure and the constant $N_2$ partial pressure to produce high pressure buildup with storage. The paste packages with $Ca(OH)_2$ maintained relatively low $CO_2$ partial pressure and thus the package pressure close to normal atmospheric pressure for initial storage period of 70 days. The packages with air pinhole channel had the partial pressures of $O_2\;and\;N_2$ decreased with storage time, while $CO_2$ partial pressure first increased to a maximum and then slowly decreased thereafter Without any pressure increase the packages with pinhole gave the lowest quality changes possibly due to the effect of package atmosphere, but it had problem of mold contamination and growth for soy paste after 120 days. There were no difference in microbial flora between the packages after about 70 day storage.

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Studies on Taste Compound Content and Reasearch on Condition of Comsumer Attitude to Traditional Korean Soy Sauce with Varing Meju Type and Fermentation Jars (메주종류와 담금용기에 따른 전통간장의 맛성분과 소비자 사용실태조사)

  • 손경희;이현주;박현경;박옥진
    • Korean journal of food and cookery science
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    • v.14 no.5
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    • pp.463-467
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    • 1998
  • This study was carried out in order to analyze the changes in general compounds, reducig sugar, nitrogen compounds of traditional Korean soy sauce with varying Meju Type (Traditional Meju, Modified Meju) and ripening periods and to investigate correlation between sensory characteistics via sensory evaluation of soy sauce samples. And to investigate actual conditions and attitude of comsumer of soy suace.

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Eggs Development ana Larvae of the Horn Fish, Hemiramphus sajori TEMMINCK et SCHLEGEL (학공치의 난발생과 부화자어)

  • KIM Yong-Uk;MYOUNG Jung-Goo;CHOI Sang-Oong
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.17 no.2
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    • pp.125-131
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    • 1984
  • The horn fish, Hemiramphus sajori TEMMINCK et SCHLEGEL, is widely distributed in the coastal waters of Korea, Japan and Taiwan. On April 13, 1983, mature adults of the horn fish were collected from Changson channel, Namhae. The authors carried out artificial insemination on the field, and incubated the eggs and reared larvae in the laboratory. And these larvae were reared for about two weeks in glass jars, fed brine shrimp nauplii. The eggs of this species are demersal and adhesive, with 4 to 6 filaments at the animal pole and a filament at the vegetal pole. The diameter of these eggs varies 1.80 to 2.00 mm. The hatching took place in ca. 376 hours after fertilization. During the incubation period water temperature fluctuated between $13.5^{\circ}C\;and\;20.0^{\circ}C$. The newly hatched larvae were elongate in shape $7.40{\sim}8.00$ mm in total length with $40+16{\sim}17=56{\sim}57$ myomers. Many melanophore were distributed on the head, both sides of dorsal body contour, peritoneum and ventral line of the tail part. Four days after hatching, the larvae were from 9.00 to 9.65 mm in total length, at this tims the larvae absorbed the yolk completely, and became postlarvae. Fourteen days after hatching, the larvae attained 13.25 mm in total length, with 14 rays of the dorsal fin, 13 rays of the anal fin, and 4 rays of pectoral fin. The lower jaw has somewhat projected.

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Component Analysis and Reproduction of a Brown Solid Cosmetic Excavated from Tomb of Princess Hwahyeop (화협옹주 출토 화장품(갈색 고체) 성분 분석 및 재현연구)

  • Lee, Chaehoon;You, Song-e;Lee, Hanhyoung;Kim, Hyoyun;Kim, Seongmi;Chung, Yongjae
    • Journal of Conservation Science
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    • v.36 no.5
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    • pp.430-439
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    • 2020
  • This study focused on analyzing solid brown cosmetic residues that were excavated from the tomb of the Hwahyeop princess. The cosmetics had been buried in 1752 in Cheonghwa Baekja (small porcelain jars) within a Huigwakham (a stone box sealed with lime). A literature search and chemical analyses of the relics were conducted at the National Palace Museum of Korea. Based on the results of these analyses, raw materials and manufacturing methods for reproduction of the cosmetic were selected and then cream-form cosmetics were prepared. Aging tests using heat, ultraviolet (UV) radiation, and CO2 were carried out to emulate the deterioration of the excavated cosmetics. Further chemical analyses and functionality tests were performed to evaluate changes from the tests and the effect as a cosmetic. As heat aging progressed, among the reproduced cosmetics, the one which has a high proposition of oil showed a color difference value of 32; the oxidation of oils involved chemical changes in O-H and C=C groups. As the cosmetic cream deteriorated under UV aging, the pH value became acidic. The result of the functional coverage test of the reproduced cosmetic cream was lower than the standard coverage ratio of modern cosmetics.

Quality Properties of Traditional Doenjang Supplemented with Extracts of Korean Herb Medicines. (국산한약재 추출물을 이용한 전통 콩된장의 품질특성)

  • 박석규;정회정;김성희;권선화;이상원
    • Journal of Life Science
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    • v.14 no.4
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    • pp.553-559
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    • 2004
  • To improve quality as a food source and functional properties of traditional fermented soybean paste (Doenjang), the Korean herb medicines (group I ∼ IV) extracted at 121$^{\circ}C$ for 2.5 hours were used as brine sources. Capsule Meju were fermented in cool brines with three different concentration (group A, B and C) of the extracts at room temperature for 3 months in large earthenware jars. The effect of traditional soybean Doenjang (refer as DHM below) supplemented with the extract of herb medicines on the food quality and chemical characteristics were examined. General composition of DHM (moisture 58.2%∼61.9%, pH 5.32∼5.56, total acidity 3.07∼3.70 ml, salt 11.7∼13.9%) did not differ from those of control (moisture 61.9%, pH 5.50, total acidity 3.25 ml, salt 12.6%). Concentration of reducing sugar, crude protein, crude lipid and amino type nitrogen were higher in DHM than those of control without addition of the extracts. Water insoluble-brown index showed no difference by the extracts. However, the former had higher water soluble-brown index than the latter, and within the DHM, groups I and II were higher than groups III and IV. Lightness values (L) of Hunter color index in groups I and II were 24.32 and 31.04, respectively. Redness values (a) of all DHMs were lower than that of control. Yellowness value (b) of groups III and IV were similar to that of control group, and Hunter b values of group I and II were lower than that of control.

Assessment of cutting time on nutrient values, in vitro fermentation and methane production among three ryegrass cultivars

  • Wang, Chunmei;Hou, Fujiang;Wanapat, Metha;Yan, Tianhai;Kim, Eun Joong;Scollan, Nigel David
    • Asian-Australasian Journal of Animal Sciences
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    • v.33 no.8
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    • pp.1242-1251
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    • 2020
  • Objective: The 3×3 factorial arrangement was used to investigate if either high water-soluble carbohydrates (WSC) cultivars or suitable time of day that the grass cut could improve nutrient values and in vitro fermentation characteristics. Methods: The 3 cultivars were mowed at 3 diurnal time points and included a benchmark WSC ryegrass cultivar 'Premium', and 2 high WSC cultivars AberAvon and AberMagic, which contained, on average, 157, 173, and 193 g/kg dry matter (DM) of WSC, and 36.0, 36.5, and 34.1 g/kg DM of N during 7th regrowth stage, respectively. The fermentation jars were run at 39℃ with gas production recorded and sampled at 2, 5, 8, 11, 14, 17, 22, 28, 36, and 48 h. The rumen liquid was collected from 3 rumen fistulated cows grazing on ryegrass pasture. Results: High WSC cultivars had significantly greater WSC content, in vitro DM digestibility (IVDMD) and total gas production (TGP), and lower lag time than Premium cultivar. Methane production for AberMagic cultivar containing lower N concentration was marginally lower than that for AberAvon and Premium cultivars. Grass cut at Noon or PM contained greater WSC concentration, IVDMD and TGP, and lower N and neutral detergent fiber (NDF) contents, but CH4 production was also increased, compared to grass cut in AM. Meanwhile, the effects of diurnal cutting time were influenced by cultivars, such as in vitro CH4 production for AberMagic was not affected by cutting time. The IVDMD and gas production per unit of DM incubated were positively related to WSC concentration, WSC/N and WSC/NDF, respectively, and negatively related to N and NDF concentrations. Conclusion: These results imply either grass cut in Noon or PM or high WSC cultivars could improve nutrient values, IVDMD and in vitro TGP, and that AberMagic cultivar has a slightly lower CH4 production compared to AberAvon and Premium. Further study is necessary to determine whether the increase of CH4 production response incurred by shifting from AM cutting to Noon and/or PM cutting could be compensated for by high daily gain from increased WSC concentration and DM digestibility.

Characteristics of Marine Litters Distribution on the Sea-bed of the East China Sea (동중국해의 해저 폐기물 분포특성)

  • Jeong, Sun-Beom;Lee, Dae-In;Cho, Hyeon-Seo;Kim, Young-Joo
    • Journal of the Korean Society for Marine Environment & Energy
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    • v.8 no.4
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    • pp.220-226
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    • 2005
  • This study evaluated the composition and distribution of marine litters on the sea-bed of the East China Sea. Surveys have been conducted by a benthic trawlnet of Dong-baek training ship of Yosu national university during the cruise of 2002-2004. Distribution density showed high value in C5 (north-western area of Jeju Island) with $110.3kg/km^2$ and those of annual mean were about $31-43kg/km^2$. Fishing gears such as nets, pots, octopus jars and etc. were about 42-72% of debris collected in the East China Sea. Composition ratio of rubber, vinyl. metal, plastic, glass, wood, cloth and etc. were within 25% except C5. Rope and drum showed strong fluctuations with 0-30% according to the trawling sites. Some vinyls and nets made in Korea, China and Japan were much collected. It is estimated that fishing gears were discarded to the sea by fishing operation, deliberately or not. An comprehensive program including continuous research, monitoring for marine litters in the Korean sea were necessary.

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Removing Contaminants from the Surface of Jagyeongnu of Changgyeonggung Palace, National Treasure No. 229 (국보 제229호 창경궁 자격루 누기 표면에 고착된 오염물 제거 방법 연구)

  • You, Ha Rim;Jo, Ha Nui;Lee, Jae Sung;Yu, Ji A;Park, Young Hwan;Ryu, Dong Wan
    • Journal of Conservation Science
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    • v.37 no.2
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    • pp.101-119
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    • 2021
  • Korea's National Treasure No. 229, the Jagyeongnu (clepsydra) of Changgyeonggung Palace, is a scientific and cultural property representing the pinnacle of science and technology in the Joseon Dynasty. Currently, only the large, mid-sie, and small Pasuhos (bronze jars) remain. During a nearly two-year conservation project by the Cultural Heritage Conservation Science Center (CHCSC) that began in 2018, conservators identified the contaminants on the surface of the water clock's components. It turned out that the contaminants had been caused by the exposure of squalane and silicone oil, used in an earlier preservation treatment, to the elements. The CHCSC conducted experiments to determine the most effective method to remove the contaminants. First, the conservators tried using an organic solvent, a poultice, and the application of toluene and bentonite, which yielded excellent reactivity and significant color difference changes (𝚫E). However, the reactivity was insufficiently effective to warrant the health hazards to the conservators and the environment (toluene is toxic). Although organic solvents required considerably more effort, time, and human resources, the conservators confirmed that their use achieved a true color difference variation (𝚫E) that was within the same range as the toxic hydrocarbon. Thus, they confirmed that using an organic surfactant was the best method for removing the contaminants.