• 한국균학회지
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• 제45권4호
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• pp.381-385
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• 2017

Sclerotinia rot was observed on Ixeridium dentatum cultivated as a succeeding crop in a garlic field in Seosan-si, Korea during the growing season in 2016 and 2017. Symptoms progressed from the initial irregular, water-soaked spots on main stems to wilting and eventually to plant death. White, cottony mycelia and black, irregular sclerotia formed on the basal stem and on soil surfaces. The optimal temperature of hyphal growth and sclerotia germination were $20^{\circ}C$ and $25^{\circ}C$, respectively. Sequence analysis of the internal transcribed spacer (ITS) regions revealed that the three strains isolated from Ixeridium dentatum are grouped with Sclerotinia sclerotiorum. Three strains were identified as Sclerotinia sclerotiorum based on morphological features, ITS sequence, and pathogenicity test. To the best of our knowledge, this work is the first report of Sclerotinia sclerotiorum causing sclerotinia rot on Ixeridium dentatum in Korea.

• 생약학회지
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• 제51권3호
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• pp.222-229
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• 2020

Recently, consume for functional cosmetics containing natural products has been greatly increased. In order to develop it as a natural cosmetic material, we selected Ixeridium dentatum (Thunb.) Tzvelev, Plantago asiatica L. and Rumex crispus L. that have antioxidant and anti-inflammatory effects. In this study, simultaneous quantitative analysis of the isolated compounds and natural product complexes (Mix.) were validated using high performance liquid chromatography (HPLC). The isolated six compounds were shown in a large linearity with a correlation coefficient (R²) of 0.99. The limit of detection (LOD) of chlorogenic acid, plantamajoside, acteoside, emodin chrysophanol and physcion were 0.36 ㎍/mL, 0.36 ㎍/mL, 0.37 ㎍/mL, 0.30 ㎍/mL, 0.22 ㎍/mL and 0.12 ㎍/mL, respectively. The limit of quantification (LOQ) of chlorogenic acid, plantamajoside, acteoside, emodin chrysophanol and physcion were 1.10 ㎍/mL, 1.08 ㎍/mL, 1.12 ㎍/mL, 0.99 ㎍/mL, 0.66 ㎍/mL and 0.35 ㎍/mL, respectively. Content analysis showed chlorogenic acid (0.19 ± 0.02%), plantamajoside (0.48 ± 0.01%), acteoside (0.65 ± 0.01%), emodin (1.15 ± 0.11%), chrysophanol (0.73 ± 0.01%) and physcion (0.69 ± 0.09%). Therefore, the results of this study may provide for basic data of standardization research natural cosmetic material development on the I. dentatum, P. asiatica and R. cripus.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.242-242
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• 2022

As the aging population increases and interest in well-being increases, the importance of developing special crops increases. Natural medicine based on the special crops has been mainly used as an adjunct therapy for many diseases and symptoms based on culture, traditional medicine, and experience. In particular, it is attracting attention as a new resource to develop new drugs such as Artemisinin, a treatment for malaria. In order to efficiently use crops, it is essential to establish omics data such as genomes, transcriptomes, and metabolites of special-purpose crops. However, many special-purpose crops have large, heterogeneous and polyploid genomes that require high cost and long time to reference genome sequencing. Therefore, we built an inexpensive, fast, but very usefill reference transcriptome as the first step. We constructed high-quality reference transcriptom sets of Codonopsis lanceolata and Ixeridium dentatum with PacBio data. Our team will continue to construct reference transcriptoms of more special-purpose crops, and the data will be released by the National Agricultural Biotechnology Information Center (NABIC) in order to be widely used in agricultural as well as medical R&D.

• 한국환경생태학회지
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• 제26권2호
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• pp.200-209
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• 2012

우리나라에 생육하는 국화과 식물들의 화기구조와 화분매개충의 상호관련성을 밝히고자, 2010년 4월부터 2011년 10월까지 꽃을 찾는 곤충들을 조사였으며 꽃들의 화기구조를 관찰하였다. 나비목 곤충이 38, 꽃등에과를 비롯한 파리목 곤충의 방화빈도 등급의 합이 38, 벌목 곤충들이 36의 높은 방화빈도를 보였다. 꽃무지과를 비롯한 딱정벌레목의 방화빈도 등급의 합이 6으로 가장 낮았다. 주요 화분매개충은 벌목 13종(꿀벌과 11종, 개미과 2종), 나비목 29종(배추흰나비과 5종, 네발나비과 12종, 뱀눈나비과 3종, 팔랑나비과 3종, 부전나비과 2종, 왕나비과 1종 및 나방류 2종), 파리목 16종(기생파리과 1종, 꽃등에과 12종 및 집파리과 1종), 딱정벌레목 6종(꽃무지과 2종, 하늘소과 2종, 잎벌레과 1종 및 꽃벼룩과 1종) 등 65종으로 나타났다 개망초는 방화곤충이 31종으로 가장 많았고, 다음으로 등골나물이 15종, 까실쑥부쟁이가 13종 순으로 낮아졌다. 솜방망이, 씀바귀, 금불초, 지느러미엉겅퀴, 곰취, 단풍취, 수리취, 고려엉겅퀴, 이고들빼기 및 산국은 모두 방화곤충이 2종으로 매우 적었다. 나비목 곤충들은 황색이나 자주색에 비하여 백색의 꽃에 상대적으로 높은 방화빈도를 보였다. 이 연구로 국화과 식물의 화기구조와 화분 매개 곤충 간의 상리공생적 상호관계를 어느 정도 파악하였다.