• 생약학회지
• /
• 제38권3호통권150호
• /
• pp.254-257
• /
• 2007

The aerial parts of Brassica juncea (Cruciferae) called by brown or oriental mustard have been widely used as a spice in food and also traditional folk medicine as stimulant, diuretic and expectorant agent. And Gatkimchi made of the aerial parts of this plant are very popular in Korea. The aerial parts of this plant was refluxed with MeOH and then fractionationed with $CH_2Cl_2$, EtOAc, n-BuOH and $H_2O$, successively. One higher alcohol compound and three flavonoids were isolated from the EtOAc fraction through silica gel and Sephadex LH-20 column chromatographies. Their structures were elucidated as n-hexacosanol(1), kaempferol(2), isorhamnetin $3-O-{\beta}-D-glucopyranoside(3)$ and isorhamnetin $3-O-(6'-O-acetyl)-{\beta}-D-glucopyranoside(4)$ by comparison of spectral data with those in references. And compounds 1 and 4 were firstly isolated from this plant.

• Asian-Australasian Journal of Animal Sciences
• /
• 제25권7호
• /
• pp.950-955
• /
• 2012

This study was conducted to determine how the isolation method of the porcine preantral follicles influenced the following follicular growth in vitro. Mechanical and enzymatical isolations were used for retrieving the follicles from prepubertal porcine ovaries, and in vitro-growth of the follicles and the expression of folliculogenesis-related genes were subsequently monitored. The enzymatic retrieval with collagenase treatment returned more follicles than the mechanical retrieval, while the percentage of morphologically normal follicles was higher with mechanical retrieval than with enzymatic retrieval. After 4 days of culture, mechanically retrieved, preantral follicles yielded more follicles with normal morphology than enzymatically retrieved follicles, which resulted in improved follicular growth. The mRNA expression of FSHR, LHR Cx43, DNMT1 and FGFR2 genes was significantly higher after culture of the follicles retrieved mechanically. These results suggest that mechanical isolation is a better method of isolating porcine preantral follicles that will develop into competent oocytes in in vitro culture.

• 한국소음진동공학회논문집
• /
• 제15권3호
• /
• pp.280-289
• /
• 2005

A single degree of freedom system and transmissibility are key concepts in many problems of vibration isolation. In order to apply this approach, however, several assumptions must be satisfied, which are often not realistic. For examples, in practical systems, vibration transmissions at multi-point with multi-degree of freedom(translational and rotational DOF) take place and mobilities or impedances of receiver structures cannot be ignored any more especially over high frequency range. Therefore, a multi-dimensional treatment is required for accurate estimation of dynamic behavior of the system. In this paper, an approach using vibrational power flow is introduced to deal with analysis of multi-dimensional vibration isolation system in a more practical way and in aspects of vibration isolations and vibration path analysis. Procedures of this approach and some results of research for vibrational power path analysis with rotational terms included are presented. Difficulties in this method are also discussed.

• Mycobiology
• /
• 제47권4호
• /
• pp.506-511
• /
• 2019

We investigated the antifungal activities of an endophytic fungus identified as Acaromyces ingoldii, found on a loblolly (Pinus taeda L.) pine bolt in Louisiana during routine laboratory microbial isolations. The specific objectives were to determine the inhibitory properties of A. ingoldii secondary metabolites (crude extract) on the mycelial growth of a brown-rot fungus Gloeophyllum trabeum and a white-rot fungus Trametes versicolor, and to determine the effective concentration of A. ingoldii crude preparation against the two decay fungi in vitro. Results show the crude preparation of A. ingoldii from liquid culture possesses significant mycelial growth inhibitory properties that are concentration dependent against the brownrot and white-rot fungi evaluated. An increase in the concentration of A. ingoldii secondary metabolites significantly decreased the mycelial growth of both wood decay fungi. G. trabeum was more sensitive to the inhibitory effect of the secondary metabolites than T. versicolor. Identification of specific A. ingoldii secondary metabolites, and analysis of their efficacy/specificity warrants further study. Findings from this work may provide the first indication of useful roles for Acaromyces species in a forest environment, and perhaps a future potential in the development of biocontrol-based wood preservation systems.

• 한국임상수의학회지
• /
• 제22권1호
• /
• pp.50-55
• /
• 2005

We investigated the contamination of animal hospital floor, beauty table, computer keyboard, exam table, operation table and forcep handle by isolations of aerobic bacteria in small animal hospitals in Gwangju. The total number of aerobic bacteria was 52 isolates and Staphylococcus spp. (38 isolates) were the predominant isolates (69.71 %) of them. The prevalent contaminated areas were floor (17 isolates), beauty table (13 isolates) and computer keyboard (9 isolates). The detection of methicillin-resistant (mecA) gene, determined by PCR, showed that 3 of the 17 coagulase-negative Staphylococcus spp. (CNS) isolates possessed the mecA gene. For evaluating the antibiotic susceptibility patterns of the isolates, disk diffusion method was used. The majority of isolates showed high susceptibility to amoxicillin (92.1 %), ceftiofur (84.2%) and polymixin B (73.7%). Also they showed the high resistant to ampicilline (66.7%), penicillin (65%) and kanamycin (56.5%). These results suggest extensive contamination of aerobic bacteria in animal hospital environment.

• 한국식물병리학회지
• /
• 제14권5호
• /
• pp.536-542
• /
• 1998

Ten isolates of the orchid mycorrhizal fungi were isolated from the roots of Korean native orchid plants (Cymbidium goeringii) which inhabitate mainly in southern and western areas of Korea. The growth rates and color of the isolates in potato dextrose agar (PDA) were various. Microscopic observations of the hyphae isolated were identified as Rhizoctonia repens and R. endophytica var endophytica or their related species. R. repens was isolated from the roots of the Korean native orchids, but R. endophytica var endophyica was only isolated from the roots of the commercial orchids introduced from foreign countries. Also, the polymorephic patterns of genomic DNA extracted from selected isolates were compared with those of DNA extracted from the orchid mycorrhizal fungi isolated previously and similar band patterns were observed among those isolates. Five isolates of R. repens were selected and cultured at the oatmeal agar for investigating their symbiosis with orchid plants. The symbiotic specificity between orchid plants and isolated orchid mycorrhizal fungi was observe by growing orchids about six months in the greenhouse. The symbiotic responses of the commercial orchid plants with selected isolates were quite different form different isolates due to the genetic variations.

• 한국전자파학회논문지
• /
• 제13권7호
• /
• pp.709-715
• /
• 2002

본 연구에서는 직접적인 급전방식인 마이크로스트립 급전과 H-shaped 개구 결합 급전을 이용해 마이크로스트립 안테나에서 최대 송수신간 전력간섭을 억제하여 송수신 분리 구현이 가능한 높은 격리도 특성을 가지면서도 최소의 기판 층 사용 및 쉬운 급전을 할 수 있는 새로운 구조의 이중급전 이중편파 안테나를 제시하고 실험을 통한 타당성을 보였다. 제안된 단일 안테나와 1$\times$4 배열 안테나의 측정된 격리도는 -49 dB와 -48 dB이하의 특성을 가졌고, 측정된 임피던스 대역폭(VSWR<1.5)은 중심 주파수 12.5 GHz에서 4 %, 중심 주파수 14.25 GHz에서 2.1 %를 보였으며, 최대 이득은 8.25 dBi와 14.2 dBi로 측정되었다.

• Journal of electromagnetic engineering and science
• /
• 제17권2호
• /
• pp.65-70
• /
• 2017

The design and implementation aspects of a new single-balanced mixer for ultra-wideband (UWB) applications are presented in this study. The proposed mixer utilizes a miniaturized UWB ring coupler as a balun, consisting of a pair of in-phase and inverted-phase transitional structures. The well-balanced UWB performance of the ring coupler, aside from the optimized diode matching, results in improved conversion loss and inter-port isolations for a wide bandwidth. The size of the implemented single-balanced diode mixer is reduced to about 60% of the area of the conventional single-balanced ring diode mixer. The measured results of the proposed mixer exhibit an average conversion loss of 7.5 dB (minimum 6.7 dB) and a port-to-port isolation of greater than 18 dB over a UWB frequency range of 3.1-10.6 GHz. The measured results agree well with the simulated results.

• 한국지진공학회논문집
• /
• 제8권2호
• /
• pp.9-17
• /
• 2004

기존 교량 중 내진 설계가 되어 있지 않은 교량의 내진 성능에 관한 평가는 향후 발생 가능한 지진에 대한 대비로써 중요하다. 성능평가 결과, 소요의 내진 성능을 보유하고 있지 못하다면 그에 대한 보강이 필요하며 보강방법으로는 여러 가지가 있다. 그 중 지진격리증기를 이용한 내진 보강은 교량에서 교각이나 기초 등에 추가적인 시공 없이 기존 받침을 교체함으로써 비교적 간단하게 수행할 수 있다. 본 논문에서는 최근 그 시공사례가 증가하고있는 지진격리증기를 기존 비내진 교량의 보강에 사용할 때 교량의 내진 성능향상을 합리적으로 규명하기 위해 기존상태의 교량 및 지진격리장치로서 내진 보강된 교량의 내진 성능을 비교하였다. 이를 위해 기존 교량 및 내진 보강된 교량에 대해 비선형 정적ㆍ동적 해석을 수행하였으며 목표 수준의 지진에 대하여 각각의 응답을 산정하고 이를 비교하여 내진 성능 향상을 확인하였다.

• 식품과학과 산업
• /
• 제50권1호
• /
• pp.2-10
• /
• 2017

Human have eaten various traditional fermented foods for a numbers of million years for health benefit as well as survival. The beneficial effects of fermented foods have been resulted from complex microbial communications within the fermented foods. Therefore, the holistic approaches for individual identification and complete microbial profiling involved in their communications have been of interest to food microbiology fields. Microbiome is the ecological community of microorganisms that literally share our environments including foods as well as human body. However, due to the limitation of culture-dependent methods such as simple isolations of just culturable microorganisms, the culture-independent methods have been consistently developed, resulting in new light on the diverse non-culturable and hitherto unknown microorganisms, and even microbial communities in the fermented foods. For the culture-independent approaches, the food microbiome has been deciphered by employing various molecular analysis tools such as fluorescence in situ hybridization, quantitative PCR, and denaturing gradient gel-electrophoresis. More recently, next-generation-sequencing (NGS) platform-based microbiome analysis has been of interest, because NGS is a powerful analytical tool capable of resolving the microbiome in respect to community structures, dynamics, and activities. In this overview, the development status of analysis tools for the fermented food microbiome is covered and research trend for NGS-based food microbiome analysis is also discussed.