• Title/Summary/Keyword: Isolation plate

검색결과 172건 처리시간 0.034초

In situ isolation and characterization of the biosurfactants of B. Subtilis

  • Akthar, Wasim S.;Aadham, Mohamed Sheik;Nisha, Arif S.
    • Advances in environmental research
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    • 제9권3호
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    • pp.215-232
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    • 2020
  • Crude oils are essential source of energy. It is majorly found in geographical locations beneath the earth's surface and crude oil is the main factor for the economic developments in the world. Natural crude oil contains unrefined petroleum composed of hydrocarbons of various molecular weights and it contains other organic materials like aromatic compounds, sulphur compounds, and many other organic compounds. These hydrocarbons are rapidly getting degraded by biosurfactant producing microorganisms. The present study deals with the isolation, purification, and characterization of biosurfactant producing microorganism from oil-contaminated soil. The ability of the microorganism producing biosurfactant was investigated by well diffusion method, drop collapse test, emulsification test, oil displacement activity, and blue agar plate method. The isolate obtained from the oil contaminated soil was identified as Bacillus subtilis. The identification was done by microscopic examinations and further characterization was done by Biochemical tests and 16SrRNA gene sequencing. Purification of the biosurfactant was performed by simple liquid-liquid extraction, and characterization of extracted biosurfactants was done using Fourier transform infrared spectroscopy (FTIR). The degradation of crude oil upon treatment with the partially purified biosurfactant was analyzed by FTIR spectroscopy and Gas-chromatography mass spectroscopy (GC-MS).

溫水수도꼭지와 온수에서 Legionella pneumophila의 分離同定 (Isolation and Identification of Legionella pneumophila from Hot Water Spouts and Hot Water)

  • Park, Ju-Hyeong;Zong, Moon-Shik
    • 한국환경보건학회지
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    • 제14권1호
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    • pp.23-32
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    • 1988
  • A study was carried out to find distribution of Legionella spp. in hot water supplying systems of buildings in Seoul. Water samples were taken from the hot water taps in three research institute buildings during the period from the 2nd to 27th February, 1987. And all of the three buildings were supplied with hot water from the same central hot water tank. GVPC BCYEa and blood agar were used for the isolation of Legionella pneurnophila and slide agglutination test (SAT) was performed to identify their strain types. Main findings were as follows 1. 44 samples taken from hot tap-water revealed 20% of positive culture and 44 samples taken from hot water spouts revealed 11% of positive culture. 2. The 78% of positive samples taken from hot water was obtained from 25.1$\circ$C~45.0$\circ$C temperature range. 3. Only, Legionella pneumophila serogroup 3 was identified and most of them has been than 2$\mu$m in length. 4. The densities of the Legionella pneurnophila isolated from hot water samples were within the range of $1.0\times 10^2$ to $1.1\times 10^3$ CFU/l and their densities isolated from hot water spouts were either 1 CFU/plate or 2 CFU/plate.

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와상전류를 응용하여 지진 충격흡수 장치를 위한 초전도 자기부상 안정화 향상 (Improving Superconductor Levitation for Seismic Isolation Device by Applying Eddy Current Effect)

  • 장형관;송준후;아시프 마흐무드;김세빈;양찬호;성태현
    • Progress in Superconductivity
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    • 제12권2호
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    • pp.93-98
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    • 2011
  • Pinning force is the mechanism between a superconductor and a permanent magnet and it provides a stable levitation. However, when external force greater than the pinning force such as the earthquake exerts, the levitated object may lose the levitating characteristic. In order to achieve more stabilized levitation, the copper plate was inserted in between a superconductor and permanent magnets. And by applying the eddy current effect caused from the relationship between a copper plate and permanent magnets, more stabilized levitation can be established. In this study, an optimized design was found based on various configurations of permanent magnet's polarity, thickness and area of copper plate, and the gap distance between copper plate and permanent magnet. As results, higher eddy current value was obtained at where the change of polarity exists in permanent magnet configuration, and the highest eddy current value was observed at the copper plate thickness of 5 mm and the area of 80 mm ${\times}$ 80 mm. From the resulted optimized conditions above, which are 7 mm gap distance between a superconductor and permanent magnets and 80 mm ${\times}$ 80 mm ${\times}$ 5 mm dimension of a copper plate, the stiffness value was 65 % increased comparing to without any copper plate insertion.

면진층을 가지는 1/10 축소된 15층 무량판 아파트건물의 진동대 실험 (Shaking Table Test of a 1/10 Scale Isolated Fifteen-story Flat Plate Apartment Building)

  • 천영수
    • 토지주택연구
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    • 제2권3호
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    • pp.287-297
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    • 2011
  • 본 논문은 적층고무형베어링을 갖는 15층 면진 무량판 아파트건물의 내진거동을 진동대실험을 통하여 검증한 결과를 제시하고 있다. 진동대실험은 중국의 CABR에서 1/10규모의 모형실험체를 대상으로 수행되었다. 실험의 진행은 4개의 지진파를 이용하여 X, Y, X+Y방향으로 다양한 크기의 지진동이 입력되었다. 실험결과, 비 면진건물은 중진레벨에서 진동주기가 현저히 감소하고, 비선형적인 거동을 보였으며, 가속도가 건물의 높이에 따라 현저하게 증가하고, 층간변위도 허용한계를 넘는 거동을 보였다. 반면, 면진건물은 중진레벨에서 거의 일정한 진동주기의 탄성적인 거동을 나타내었으며, 지진하중과 층 가속도가 현저하게 감소하는 응답을 나타내었다. 또한 면진층의 변위는 허용범위 내에서 거동하고, 층간변위는 무시할 수 있을 만큼 작은 강체거동을 나타내었다. 결론적으로 면진은 건물에 대한 지진의 영향을 감소시키는데 매우 효과적이며, 층 가속도의 감소를 통하여 사용성을 증가시키고, 건물 내 설비 등을 안전하게 보호할 수 있음이 입증되었다.

Fe2O3가 코팅된 판상 mica의 적외선 반사용 적색안료 제조 및 차열도료의 열특성 (Preparation of Fe2O3 Coated on Mica for Infrared Reflectance Red Pigment and Thermal Property of Its Isolation-Heat Paint)

  • 이현진;김대성;이승호;임형미;최병기;강광중;정재일;조금성
    • 한국재료학회지
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    • 제25권2호
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    • pp.61-67
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    • 2015
  • $Fe_2O_3$ coated plate mica($Fe_2O_3$/mica) for infrared reflectance red pigment was prepared under hydrothermal treatment. $Fe_2O_3$ was perfectly coated on mica via the difference of surface charge between $Fe_2O_3$ and mica particles at pH 3. $Fe_2O_3$/mica was then calcined at $800^{\circ}C$ to stabilize the coated layer on mica. The infrared(IR) reflectance pigments were characterized by X-ray diffraction, FE-SEM, zeta potential, and a UV-Vis-NIR spectrophotometer. In particular, the CIE color coordinate and IR reflectance properties of $Fe_2O_3$/mica pigments were investigated in relation to the thickness variation of the $Fe_2O_3$ layer coated on mica of various lateral sizes. The isolation-heat red paints containing the pigments were prepared and optimized with a thinner, settling agent, and dispersant. Then, the films were made. The thermal property of isolation-heat on these films was observed through the relationship of the IR reflectance value, which was based on the variation of the $Fe_2O_3$ layer's thickness coated on mica and mica's lateral size as IR reflectance pigment. With an increase in IR reflectance on these films, the thermal property of isolation-heat was effectively enhanced.

한국인의 급성 진행성치주염환자 치은연하 균태 Bacteroides gingivalis균주분리에 관한 연구(I) (ISOLATION AND IDENTIFICATION OF BACTEROIDES GINGIVALIS OF KOREAN RAPIDLY PROGRESSIVE PERIODONTITIS)

  • 정종평;이영희;최주용;손재운;손성희;최상묵;한수부
    • 대한치과의사협회지
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    • 제22권10호통권185호
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    • pp.865-868
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    • 1984
  • Three Korean rapidly progressive periodontitis patient (R.P.P.) were examined for the isolation and identification of black-pigmented Bacteroides and Bacteroides gingivalis in the subgingival plaque. Black-pigmented Bacteroides and Bacteroides gingivalis were recovered from all three R.P. patients and the predominant isolates were Bacteroides intermedius followed by Bacteroides gingivalis. The B. gingivalis isolated from subgingival plaque of rapidly progressive periodontitis patients were Gram-negative pleomorphic rods and each produced brown to black colonies on blood agar plate supplemented with 5% rabbit 5ug/ml haemin, 0.5ug/ml of menadione and 40ug/ml of Kanamycin. The B. gingivalis isolated were fermented none of Glucose, Sucrose, Lactose, Esculin and Cellobinose and produced indole. Isolation and identification of Bacteroides gingivalis in Korean R.P. patients can be contributed to the research on the pathogenesis of Korean periodontal disease.

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LNG 저장 탱크의 지진방지를 위한 면진베어링(LRB)의 개발 (Development of Isolators (Laminated Rubber Bearing) for the Seismic Isolation of LNG Storage Tanks)

  • 유춘화;김두훈;이동근;김남식;정우정
    • 한국소음진동공학회:학술대회논문집
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    • 한국소음진동공학회 1994년도 춘계학술대회논문집; 영남대학교, 20 May 1994
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    • pp.116-121
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    • 1994
  • 지금까지 대형 구조물에 널리 사용되고 있는 Base Isolator는 특히, 지진하중에 대하여 상부 구조물의 지반분리(base isolation)을 이용한 지진제어를 목적으로 하기 때문에 Seismic Isolator라 한다. 일반적으로 면진베어링에는 LRB(Laminated Rubber Bearing) 방식과 R-FBI(resilient-friction base isolator) 방식으로 크게 나눌 수 있다. LRB 방식은 가장 널리 사용되는 면진베어링으로써 방진고무를 주된 재료로 하고 수직강성을 보강하기 위하여 steel plate를 조합하여 제작하며, 초기강성 및 에너지 소산능력을 증가시키기 위하여 단면중앙에 납(lead plug)를 삽입하기도 한다. R-FBI 방식은 방진고무 적층판 내부에 미끄럼판을 가지고 있으므로 LRB 방식에 비하여 더 큰 수평변위를 발생시킬 수 있다. 이번에 설계 제작한 면진베어링은 LNG 저장탱크의 Seismic Isolation을 목적으로 적용대상의 사양에 맞추어 설계 제작하고 성능평가 시험을 수행하여 LNG 저장탱크, 원자로, 대형 건축물등 지진으로부터 보호되어야 하는 대형구조물의 방진재로 적용할 수 있는가를 평가하고자 한다. 본 논문에서는 제작된 면진베어링의 설계를 검증하고 방진고무(HDR)재료의 물리적 특성시험, 축소모델에 의한 정적, 동적시험을 통하여 시험방법을 소개하고 이러한 시험결과를 기초로 하여 면진베어리의 성능을 평가하였으며, 면진베어링의 온도변화, 외부 수직하중의 변화등에 따른 특성변화와 LNG 저장탱크와 면진베어링의 체결방법에 따른 시험으로 체결방법을 검증하였으며, 대상물의 사양에 적합한가를 고찰하였다.

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능동 고립 시스템의 성능 실험 (Experiment on Performance of Vibration Isolation System)

  • 김승록;허석;곽문규
    • 한국소음진동공학회:학술대회논문집
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    • 한국소음진동공학회 2003년도 춘계학술대회논문집
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    • pp.142-147
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    • 2003
  • This paper is concerned with the experiment of vibration isolation device whit can cope with heave, pitch and roll type vibrations. This new device consists of S-shaped piezoceramic actuators supporting each corner of the plate. Small piezoceramic sensors are located at the middle of each side. This paper also presents the development and the movement of the system. The active control technique was also developed to cope with base excitation. Experimental results show that vibrations can be reduced by the new device in the case of base excitation of harmonic type.

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Antinocicepetive Effects of 3,4-Dicaffeoyl Quinic Acid of Ligularia fischeri var. spiciformis

  • Choi, Moo-Young;Park, Hee-Juhn
    • 한국자원식물학회지
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    • 제20권3호
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    • pp.221-225
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    • 2007
  • The plant Ligularia fischeri var. spiciformis (Compositae) is a candidate for available functional foods. It has been used to treat diabetes mellitus and rheumatoid arthritis. We have reported the isolation of a new eremophilanolide named 6-oxoeremophilenolide and cytotoxic intermedeol together with the isolation of hydrophilic constituents, chlorogenic acid, 3,4-di-O-caffeoylquinic acie (3), and 5-O-[1-butyl]-3,4-di-O-caffeoylquinic acid. Compound 3 was again isolated by combination of silica gel- and ODS column chromatography for the anti-nociceptive action. Compound 3 and 4 were assayed in hot plate- and writhing tests in the rat. Although the three derivatives of caffeic acid exhibited significant anti-nociceptive effects at 10 mg/kg dose (i.p.),(activity potency: 4>3). These results suggest that compound 3 is responsible for at least rheumatoid arthritis, and caffeic acid moiety is the active moiety of dicaffeoylquinic acid.

고성능 용량 형 지문센서 신호처리 회로 설계 (High Performance Circuit Design of a Capacitive Type Fingerprint Sensor Signal Processing)

  • 정승민;이문기
    • 대한전자공학회논문지SD
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    • 제41권3호
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    • pp.109-114
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    • 2004
  • 본 논문에서는 반도체 방식의 직접 터치식 용량 형 지문인식센서의 신호처리를 위한 회로를 제안하였다. 센서로부터의 용량의 변화를 전압의 신호로 전환하기 위해서 전하분할 방식의 회로를 적용하였다. 지문센서 감도저하의 가장 큰 원인인 센서 플레이트에 존재하는 기생용량을 제거하고 융선과 계곡 사이의 전압차를 향상시키기 위하여 기존과는 다른 아날로그 버퍼회로를 설계 적용하였다. 센서 하부회로와의 isolation 대책을 통하여 ESD 및 노이즈방지를 위한 설계를 실시하였다. 제안된 신호 처리회로는 0.35마이크론 표준 CMOS 공정에 의해 레이아웃 되었다.