• Advances in environmental research
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• v.9 no.3
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• pp.215-232
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• 2020

Crude oils are essential source of energy. It is majorly found in geographical locations beneath the earth's surface and crude oil is the main factor for the economic developments in the world. Natural crude oil contains unrefined petroleum composed of hydrocarbons of various molecular weights and it contains other organic materials like aromatic compounds, sulphur compounds, and many other organic compounds. These hydrocarbons are rapidly getting degraded by biosurfactant producing microorganisms. The present study deals with the isolation, purification, and characterization of biosurfactant producing microorganism from oil-contaminated soil. The ability of the microorganism producing biosurfactant was investigated by well diffusion method, drop collapse test, emulsification test, oil displacement activity, and blue agar plate method. The isolate obtained from the oil contaminated soil was identified as Bacillus subtilis. The identification was done by microscopic examinations and further characterization was done by Biochemical tests and 16SrRNA gene sequencing. Purification of the biosurfactant was performed by simple liquid-liquid extraction, and characterization of extracted biosurfactants was done using Fourier transform infrared spectroscopy (FTIR). The degradation of crude oil upon treatment with the partially purified biosurfactant was analyzed by FTIR spectroscopy and Gas-chromatography mass spectroscopy (GC-MS).

• Journal of Environmental Health Sciences
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• v.14 no.1
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• pp.23-32
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• 1988

A study was carried out to find distribution of Legionella spp. in hot water supplying systems of buildings in Seoul. Water samples were taken from the hot water taps in three research institute buildings during the period from the 2nd to 27th February, 1987. And all of the three buildings were supplied with hot water from the same central hot water tank. GVPC BCYEa and blood agar were used for the isolation of Legionella pneurnophila and slide agglutination test (SAT) was performed to identify their strain types. Main findings were as follows 1. 44 samples taken from hot tap-water revealed 20% of positive culture and 44 samples taken from hot water spouts revealed 11% of positive culture. 2. The 78% of positive samples taken from hot water was obtained from 25.1$\circ$C~45.0$\circ$C temperature range. 3. Only, Legionella pneumophila serogroup 3 was identified and most of them has been than 2$\mu$m in length. 4. The densities of the Legionella pneurnophila isolated from hot water samples were within the range of $1.0\times 10^2$ to $1.1\times 10^3$ CFU/l and their densities isolated from hot water spouts were either 1 CFU/plate or 2 CFU/plate.

• Progress in Superconductivity
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• v.12 no.2
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• pp.93-98
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• 2011

Pinning force is the mechanism between a superconductor and a permanent magnet and it provides a stable levitation. However, when external force greater than the pinning force such as the earthquake exerts, the levitated object may lose the levitating characteristic. In order to achieve more stabilized levitation, the copper plate was inserted in between a superconductor and permanent magnets. And by applying the eddy current effect caused from the relationship between a copper plate and permanent magnets, more stabilized levitation can be established. In this study, an optimized design was found based on various configurations of permanent magnet's polarity, thickness and area of copper plate, and the gap distance between copper plate and permanent magnet. As results, higher eddy current value was obtained at where the change of polarity exists in permanent magnet configuration, and the highest eddy current value was observed at the copper plate thickness of 5 mm and the area of 80 mm ${\times}$ 80 mm. From the resulted optimized conditions above, which are 7 mm gap distance between a superconductor and permanent magnets and 80 mm ${\times}$ 80 mm ${\times}$ 5 mm dimension of a copper plate, the stiffness value was 65 % increased comparing to without any copper plate insertion.

• Land and Housing Review
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• v.2 no.3
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• pp.287-297
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• 2011

This paper presents the results of performance verification tests of the isolated flat plate apartment building with the laminated rubber bearings. The shaking table test is carried out in CABR(China Academy of Building Research) with two 1/10 scale isolation and non-isolation models under 4 excitation waves. The shaking table test is proceeding from x axis, y axis and x+y axis with different amplitude of acceleration values. The results show that, to non-isolated model, the natural vibration period is remarkably decreased and entered non-linear condition after moderate earthquake. Its accelerations become lager with increasing storey number and completely collapsed under large earthquake. The inter-storey shifts largely exceed the limit values of regulated displacement angles. But to isolated model, the natural vibration period of isolated modal is almost the same in all conditions and still in its elastic condition. The earthquake loading is greatly reduced and the accelerations of superstructure are greatly reduced. The inter-storey drifts are very small and can be neglected. The isolated model is in translational state and can be seen as a rigid whole. The displacements of isolation layer are in the allowable range. This experiment demonstrates that the seismic isolation is very effective to mitigate the influence of earthquake on structures and it is possible to increase the serviceability due to decrease the floor acceleration. facilities from their good states that is superior to non-isolated structure.

• Korean Journal of Materials Research
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• v.25 no.2
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• pp.61-67
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• 2015

$Fe_2O_3$ coated plate mica($Fe_2O_3$/mica) for infrared reflectance red pigment was prepared under hydrothermal treatment. $Fe_2O_3$ was perfectly coated on mica via the difference of surface charge between $Fe_2O_3$ and mica particles at pH 3. $Fe_2O_3$/mica was then calcined at $800^{\circ}C$ to stabilize the coated layer on mica. The infrared(IR) reflectance pigments were characterized by X-ray diffraction, FE-SEM, zeta potential, and a UV-Vis-NIR spectrophotometer. In particular, the CIE color coordinate and IR reflectance properties of $Fe_2O_3$/mica pigments were investigated in relation to the thickness variation of the $Fe_2O_3$ layer coated on mica of various lateral sizes. The isolation-heat red paints containing the pigments were prepared and optimized with a thinner, settling agent, and dispersant. Then, the films were made. The thermal property of isolation-heat on these films was observed through the relationship of the IR reflectance value, which was based on the variation of the $Fe_2O_3$ layer's thickness coated on mica and mica's lateral size as IR reflectance pigment. With an increase in IR reflectance on these films, the thermal property of isolation-heat was effectively enhanced.

• The Journal of the Korean dental association
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• v.22 no.10 s.185
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• pp.865-868
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• 1984

Three Korean rapidly progressive periodontitis patient (R.P.P.) were examined for the isolation and identification of black-pigmented Bacteroides and Bacteroides gingivalis in the subgingival plaque. Black-pigmented Bacteroides and Bacteroides gingivalis were recovered from all three R.P. patients and the predominant isolates were Bacteroides intermedius followed by Bacteroides gingivalis. The B. gingivalis isolated from subgingival plaque of rapidly progressive periodontitis patients were Gram-negative pleomorphic rods and each produced brown to black colonies on blood agar plate supplemented with 5% rabbit 5ug/ml haemin, 0.5ug/ml of menadione and 40ug/ml of Kanamycin. The B. gingivalis isolated were fermented none of Glucose, Sucrose, Lactose, Esculin and Cellobinose and produced indole. Isolation and identification of Bacteroides gingivalis in Korean R.P. patients can be contributed to the research on the pathogenesis of Korean periodontal disease.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 1994.04a
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• pp.116-121
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• 1994

지금까지 대형 구조물에 널리 사용되고 있는 Base Isolator는 특히, 지진하중에 대하여 상부 구조물의 지반분리(base isolation)을 이용한 지진제어를 목적으로 하기 때문에 Seismic Isolator라 한다. 일반적으로 면진베어링에는 LRB(Laminated Rubber Bearing) 방식과 R-FBI(resilient-friction base isolator) 방식으로 크게 나눌 수 있다. LRB 방식은 가장 널리 사용되는 면진베어링으로써 방진고무를 주된 재료로 하고 수직강성을 보강하기 위하여 steel plate를 조합하여 제작하며, 초기강성 및 에너지 소산능력을 증가시키기 위하여 단면중앙에 납(lead plug)를 삽입하기도 한다. R-FBI 방식은 방진고무 적층판 내부에 미끄럼판을 가지고 있으므로 LRB 방식에 비하여 더 큰 수평변위를 발생시킬 수 있다. 이번에 설계 제작한 면진베어링은 LNG 저장탱크의 Seismic Isolation을 목적으로 적용대상의 사양에 맞추어 설계 제작하고 성능평가 시험을 수행하여 LNG 저장탱크, 원자로, 대형 건축물등 지진으로부터 보호되어야 하는 대형구조물의 방진재로 적용할 수 있는가를 평가하고자 한다. 본 논문에서는 제작된 면진베어링의 설계를 검증하고 방진고무(HDR)재료의 물리적 특성시험, 축소모델에 의한 정적, 동적시험을 통하여 시험방법을 소개하고 이러한 시험결과를 기초로 하여 면진베어리의 성능을 평가하였으며, 면진베어링의 온도변화, 외부 수직하중의 변화등에 따른 특성변화와 LNG 저장탱크와 면진베어링의 체결방법에 따른 시험으로 체결방법을 검증하였으며, 대상물의 사양에 적합한가를 고찰하였다.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2003.05a
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• pp.142-147
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• 2003

This paper is concerned with the experiment of vibration isolation device whit can cope with heave, pitch and roll type vibrations. This new device consists of S-shaped piezoceramic actuators supporting each corner of the plate. Small piezoceramic sensors are located at the middle of each side. This paper also presents the development and the movement of the system. The active control technique was also developed to cope with base excitation. Experimental results show that vibrations can be reduced by the new device in the case of base excitation of harmonic type.

• Korean Journal of Plant Resources
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• v.20 no.3
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• pp.221-225
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• 2007

The plant Ligularia fischeri var. spiciformis (Compositae) is a candidate for available functional foods. It has been used to treat diabetes mellitus and rheumatoid arthritis. We have reported the isolation of a new eremophilanolide named 6-oxoeremophilenolide and cytotoxic intermedeol together with the isolation of hydrophilic constituents, chlorogenic acid, 3,4-di-O-caffeoylquinic acie (3), and 5-O-[1-butyl]-3,4-di-O-caffeoylquinic acid. Compound 3 was again isolated by combination of silica gel- and ODS column chromatography for the anti-nociceptive action. Compound 3 and 4 were assayed in hot plate- and writhing tests in the rat. Although the three derivatives of caffeic acid exhibited significant anti-nociceptive effects at 10 mg/kg dose (i.p.),(activity potency: 4>3). These results suggest that compound 3 is responsible for at least rheumatoid arthritis, and caffeic acid moiety is the active moiety of dicaffeoylquinic acid.

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.41 no.3
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• pp.109-114
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• 2004

This paper proposes an advanced circuit for the fingerprint sensor signal processing. We increased the voltage between ridge and valley by modifying the parasitic capacitance eliminating circuit of sensor plate. The analog comparator was designed for comparing the sensor signal voltage with the reference signal voltage. We also propose an effective isolation strategy for removing noise and signal coupling of each sensor pixel. The fingerprint sensor circuit was designed and simulated, and the layout was performed.