• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.136.1-136
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• 2003

Bacterial grain rot by Burkholderia gluae cause severe damage in seedling and grain of rice after heading season. This seed-borne pathogen play a role as first infection agent that could be cause disease following cropping season. Until now the direct isolation of the bacteria has some trouble by interference of other bacteria existed inside seed. This study established convenient identification method as simple isolation with KB medium from seed showing symptom and using PCR identification. By this isolation method, B. glumae was isolated from 40 to 50％ in brown rice and inner hull, however, there were saprophytic bacteria and fungi outer hull. In PCR identification with Ogf4 and Ogr3 primer to these 25 isolates, the amplified products were presented in all of the collections but not in 10 saprophytic germs. The isolation rate was constant to 3 months stored seeds. This result provide a rapid and convenient isolation and identification of B. glumae.

• Journal of the Korea Concrete Institute
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• v.20 no.5
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• pp.643-651
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• 2008

The purpose of this study is to evaluate seismic capacity of seismically isolated building according to the earthquake motion record selection method. To analyze the seismic behavior, 20-story building is designed, which has base isolation system. The using earthquake motion record were selected by two categories. The one is a proposed earthquake record according to soil type and response spectrum shape, and the other is a well known earthquake events such as El Centro (1940). The time history analysis results of base isolation buildings be induced difference results according to each ground motion records. Therefore detailed guidelines for the ground motion records selection method must be prepared. And the response of isolation story displacement and shear force show good seismic performance in consideration of the proposed earthquake records.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.6 no.11
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• pp.2764-2783
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• 2012

Network virtualization supports future Internet environments and cloud computing. Virtualization can mitigate many hardware restrictions and provide variable network topologies to support variable cloud services. Owing to several advantages such as low cost, high flexibility, and better manageability, virtualization has been widely adopted for use in network virtualization platforms. Among the many issues related to cloud computing, to achieve a suitable cloud service quality we specifically focus on network and performance isolation schemes, which ensure the integrity and QoS of each virtual cloud network. In this study, we suggest a virtual network platform that uses Xen-based virtualization, and implement multiple virtualized networks to provide variable cloud services on a physical network. In addition, we describe the isolation of virtual networks by assigning a different virtualized network ID (VLAN ID) to each network to ensure the integrity of the service contents. We also provide a method for efficiently isolating the performance of each virtual network in terms of network bandwidth. Our performance isolation method supports multiple virtual networks with different levels of service quality.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.19 no.8
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• pp.820-825
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• 2008

A method to improve isolation characteristics of internal MIMO antenna systems for wireless portable devices operating in the $2.3{\sim}2.4$ GHz band is presented. The proposed system incorporates multiple pairs of L-slots between the two antennas in the ground plane, which operate like a band-stop filter, suppressing mutual coupling between the antennas and resulting in improved isolation. A MIMO antenna system with 6 pairs of L-slots shows reflection loss of -26.4 dB and isolation of -37.5 dB.

• Microbiology and Biotechnology Letters
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• v.24 no.2
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• pp.178-184
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• 1996

Dryfilm method by using 3M Petrifilm$^{TM}$ has been examined to replace conventional agar method for isolation of microorganisms from foods. The objectives of the present study were to evaluate suitability of dryfilm method as a microbial isolation method and to determine the effect of antimicrobial agent on dryfilm for isolation of microorganisms from foods. Five different foods, milk, ground beef, fishery surimi, Takju and wheat flour were used to isolate the natural microflora in foods and the inoculated Escheri chia coli. Standard method agar (SMA, Difco) and Petrifilm$^{TM}$ aerobic count (PAC, 3M) were used to isolate total microorganisms from foods. Violet red bile agar (VRBA), brilliant green lactose bile (BGLB) broth and Petrifilm$^{TM}$ coliform count (PCC, 3M) were used to isolate coliforms from foods. E. coli broth (EC broth) and Petrifilm$^{TM}$ E. coli count (PEC, 3M) were used to isolate E. coli from foods. Acidified potato dextrose agar (APDA) and Petrifilm$^{TM}$ yeast & mold count (PYMC, 3M) were used to isolate yeasts and molds from foods. Total aerobic plate counts isolated from five different foods by SMA and PAC (3M) were riot significantly different each other at P<0.05 level and were highly correlated each other ($\geq$0.96). Mugwort extract as an antimicrobial agent did not affect microbial enumeratiion of Dryfilm. Significantly higher number of coliform colonies were formed on VRBA than PCC (3M) from ground beef, but they were not significantly different in coliform colonies from milk samples. PCC (3M) and BGLB were not significantly different for enumeration of coliforms in milk and beef samples. Significantly higher number of E. coli were isolated by EC broth than PEC from ground beef, but these were not significontly different for enumeration of E. coli from milk. Yeast and mold counts isolated from Takju and wheat flour by APDA and PYMC (3M) were not significantly different at P<0.05 level. These data indicate that dryfilm method by using 3M Petrifilm$^{TM}$ can be successively used as an alternative to conventional agar method for enumeration of microorganisms in various foods.

• Journal of Power Electronics
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• v.17 no.1
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• pp.305-313
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• 2017

A novel interference isolation method is proposed by using several designed coils in capacitive power transfer systems as isolation impedances. For each designed coil, its stray parameters such as the inter-turn capacitance, coil resistance and capacitance between the coil and the core, etc. are taken into account. An equivalent circuit model of the designed coil is established. According to this equivalent circuit, the impedance characteristic of the coil is calculated. In addition, the maximum impedance point and the corresponding excitation frequency of the coil are obtained. Based on this analysis, six designed coils are adopted to isolate the interference from power delivery. The proposed method is verified through experiments with a power carrier frequency of 1MHz and a data carrier frequency of 8.7MHz. The power and data are transferred parrallelly with a data carrier attenuation lower than -5dB and a power attenuation on the sensing resistor higher than -45dB.

• Journal of Microbiology and Biotechnology
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• v.11 no.5
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• pp.890-894
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• 2001

The isolation of genomic DNA from mammalian cells is usually performed by cell lysis followed by protein digestion, extraction, and finally, ethanol precipitation of the chromosomal DNA. However, in the case of large sample numbers or when only small amounts of starting materials are available, such conventional methods are not efficient and are cumbersome to be applied. Some alternative methods have been described as well as having commercial DNA isolation kits to be available, nevertheless, there is room left for much improvement. In the present study, a novel method is introduced, where it simplifies conventional protocols by omitting some time-consuming steps such as protease incubation or DNA precipitation and its resuspension. Using paramagnetic silica resins, the genomic DNA was purified over a magnetic field, and the bound DNA was eluted with a low-salt buffer. The fidelity and effectiveness of this novel method was determined by using normal and apoptotic cells as a starting material and then compared to other protocols. The high speed and convenience along with its high efficiency in detecting apoptotic chromosomal DNA will prove this method to be an improved alternative in the isolation of genomic DNA from mammalian cells.

• Journal of Embryo Transfer
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• v.33 no.4
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• pp.327-336
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• 2018

To date, there are no protocols optimized to the effective separation of spermatogonial stem cells (SSCs) from testicular cells derived from mouse testes, thus hindering studies based on mouse SSCs. In this study, we aimed to determine the most efficient purification method for the isolation of SSCs from mouse testes among previously described techniques. Isolation of SSCs from testicular cells derived from mouse testes was conducted using four different techniques: differential plating (DP), magnetic-activated cell sorting (MACS) post-DP, MACS, and positive and negative selection double MACS. DP was performed for 1, 2, 4, 8, or 16 h, and MACS was performed using EpCAM ($MACS^{EpCAM}$), Thy1 ($MACS^{Thy1}$), or GFR ${\alpha}1$ ($MACS^{GFR{\alpha}1}$) antibodies. The purification efficiency of each method was analyzed by measuring the percentage of cells that stained positively for alkaline phosphatase. DP for 8 h, $MACS^{Thy1}$ post-DP for 8 h, $MACS^{GFR{\alpha}1}$, positive selection double $MACS^{GFR{\alpha}1/EpCAM}$, and negative selection double $MACS^{GFR{\alpha}1/{\alpha}-SMA}$ were identified as the optimal protocols for isolation of SSCs from mouse testicular cells. Comparison of the purification efficiencies of the optimized isolation protocols showed that, numerically, the highest purification efficiency was obtained using $MACS^{GFR{\alpha}1}$. Overall, our results indicate that $MACS^{GFR{\alpha}1}$ is an appropriate purification technique for the isolation of SSCs from mouse testicular cells.

• Journal of Ginseng Research
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• v.10 no.1
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• pp.21-26
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• 1986

Polyacetylenes (conjugated poly-ynes) were Isolated from the roots of Panax Ginseng C.A. Meyer by the low pressure preparative liquid chromatography and characterized by spectral analyses. The advantages of the isolation method are the simplicity of the isolation procedure and the much higher yield of poly-ynes compared to the old method.

• Journal of Institute of Control, Robotics and Systems
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• v.11 no.3
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• pp.200-206
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• 2005

In this paper, a redundancy management system for aircraft is studied, and FDI (Fault Detection and Isolation) algorithm of inertial sensor system is proposed. UAV system cannot allow triple or quadruple hardware redundancy due to the limitations on space and weight. In the UAV system with dual sensors, it is very difficult to identify the faulty sensor. Also, conventional FDI method cannot isolate multiple faults in a triple redundancy system. In this paper, hardware based FDI technique is proposed, which combines a parity equation approach with the wavelet based technique, which is a model-free FDI method. To verify the effectiveness of the proposed FDI method, numerical simulations are performed.