• 대한수의학회지
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• 제39권5호
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• pp.995-1005
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• 1999

Staphylococcus aureus is one of most prevalent intramammary pathogens and have characteristics which are not easily eradicated. Recently, to understand the sources and transmission of S aureus, many studies have focused on the subtyping of field isolate. This study was preformed to investigate the distribution pattern and characteristics of the isolates using phenotyping and genotyping. Samples were collected from milk of each udder, cow bodies (perianal region, vagina, tail, udder skin, sole) and environment (floor, liner, milker's hands, water, towel, insect) from 6 herds located in Kyung-gi province. Forty five strains of S aureus were isolated from 3 dairy herds (A, B, C) and were typed by hemolytic pattern, antibiotic resistant pattern, enterotoxin typing and PCR-based DNA fingerprinting. Slime productivity was also compared by each subtype to examine potential infectiousness. Of 45 strains, 41 were isolated from milk samples and 4 were isolated from liners. No strains isolated in the bodies and environment. Forty five strains isolated were classified as 18 subtypes by phenotyping and genotyping. There was common subtype between A and B herd, but the subtype of C herd showed different pattern. Among predominant subtypes, 60% of S aureus strain isolated from A and B herd showed subtype I and 50% of S aureus strain isolated from C herd belonged to subtype VI and X II. Neither somatic cell count (SCC) nor slime production was significantly different between predominant and minor subtypes. In summary, the study revealed that liners play more important roles in the mode of transmission than environmental sources. Several subtypes can be found in a herd, only a few subtype, however, was largely associated with the majority of infection.

• 대한수의학회지
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• 제14권2호
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• pp.207-210
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• 1974

Pharmacological investigation was performed in chickens which were pretreated with reserpine. Transmural stimulations were given to the isolated jejunum of chickens and studied the responses and the effects of dibenamine, propranolol, atropine and tetrodotoxin on them. The results obtained were summerized as follows: 1. Three different patterns of response were obtained from the isolated jejunum of non-treated chickens after giving them transmural stimulation. The first pattern was contraction followed by relaxation, the second pattern was contraction only and the third pattern was relaxation only. 2. The transmural stimulation of the jejunum preparations evoked relaxation while the periarterial stimulation evoked contraction in the reserpinized chick. 3. The relaxation response to the transmural stimulation was not affected by the pretreatment with dibenamine, propranolol and atropine. 4. The relaxation response to the transmural stimulation was abolished by tetrodotoxin. The results obtained in these studies indicate that the relaxation response to the transmural stimulation is due to non-adrenergic inhibitory fibers.

• 대한한의학원전학회지
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• 제22권4호
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• pp.29-32
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• 2009

We have explored the five-element pattern of "River Table(河圖)" in the "Naegyeong(內經)", and conclude that this pattern is one of the five-element patterns applied in the "Naegyeong". Based on this pattern, we can exactly understand the eum(陰) and yang(陽) range of five Jang-organs, why the spleen is the extreme eum, why the spleen is the isolated Jang, how the spleen governs four seasons, the ascending and descending of visceral gi(氣), as well as why it can be applied in clinic that the spleen and stomach are the pivot of the ascending and descending of visceral gi(氣).

• 한국환경보건학회지
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• 제30권1호
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• pp.15-21
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• 2004

In an attempt to analyze the characteristics of domestic pathogenic strains of S. pneumoniae, the basic epidemiological charactristics of pathogenic strains such as their serotypes and frequency of penicillin resistance, and pattern of chromosomal DNA from PFGE(pulsed-field gel electrophoresis) were observed. For this study,56 strains of S. pneumoniae isolated from inpatients and outpatients in the four domestic university hospitals were collected from January to December in 1998. Among those strains, a total of 56 pathogenic strains from blood(39 isolates), cerebrospinal fluid(8 isolates) and other specimen(9 isolates) were selected and isolated. The penicillin resistance frequency of those 56 strains was identified with disk diffusion method with 66.1％. From the invasive strains, predominant serotypes were isolated in the order of 19F(12.5％), 23F(10.7%), 14(10.7%) and 9V(10.7％), totalling 45 percent. This experiment also used PFGE patterns to compare the correlations among genetic subtypes in several serotypes. The DNA fragments digested with Sma I and Apa I were resolved by PFGE. The PFGE patterns digested with Sma I were better than Apa I for analysis. In the DNA fragments digested with Sma 1, PFGE analysis of 56 S. pneumoniae isolates showed 25 different patterns. As a result, serotype was on the whole correlated to PFGE pattern on the ground that each different PFGE pattern by serotype was observed. This study can be utilized not only fur the study of incidence trend of domestic pneumococcal diseases but also as a useful basic data for the development of identification tool and treatment.

• 한국음향학회:학술대회논문집
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• 한국음향학회 1993년도 학술논문발표회 논문집 제12권 1호
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• pp.121-124
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• 1993

This paper is a study on the word recognition using neural network. A limited vocabulary, speaker independent, isolated word recognition system has been built. This system recognizes isolated word without performing segmentation, phoneme identification, or dynamic time wrapping. It needs a static pattern approach to recognize a spatio-temporal pattern. The preprocessing only includes preceding and tailing silence removal, and word length determination. A LPC analysis is performed on each of 24 equally spaced frames. The PARCOR coefficients plus 3 other features from each frame is extracted. In order to simplify a structure of neural network, we composed binary code form to decrease output nodes.

• 한국음향학회지
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• 제3권1호
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• pp.25-35
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• 1984

This paper analysis the Dynamic Time Warping algorithms for time normalization of speech pattern and discusses the Dynamic Programming algorithm for spoken Korean isolated digits recognition. In the DP matching, feature vectors of the reference and test pattern are consisted of first three formant frequencies extracted by power spectrum density estimation algorithm of the ARMA model. The major differences in the various DTW algorithms include the global path constrains, the local continuity constraints on the path, and the distance weighting/normalization used to give the overall minimum distance. The performance criterias to evaluate these DP algorithms are memory requirement, speed of implementation, and recognition accuracy.

• Korean Chemical Engineering Research
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• 제59권1호
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• pp.94-99
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• 2021

2단 Rushton turbine 날개를 장착한 접시바닥형 교반조 내의 유동 상태가 어떤 회전수에서 변화하는 현상을 찾아냈다. 층류 영역에서는 회전수를 변화시켜도 안정적인 도넛 링이 형성되어 혼합 패턴에 특이한 변동은 관찰되지 않았다. 전이영역에서의 평바닥형 교반조에서는 회전수에 변화를 주어도 두꺼운 2개의 미혼합 도넛 링에 변화가 없는 반면, 접시바닥형 교반조에서는 회전수가 450 rpm이 되면 두꺼운 2개의 미혼합 도넛 링이 아주 얇은 3개의 도넛 링으로 바뀌어 혼합이 개선되었다. 접시바닷형 교반조에서는 Re=138~178의 영역에서 링 모양의 미혼합 영역이 3곳에서 나타났고 그 크기도 컸지만, 평바닥형 교반조에서는 Re=116~176에서 링모양의 미혼합 영역은 2곳에서 나타났으며 그 크기도 작았다. 이러한 현상이 관찰되는 조건은 전이영역으로, 방해판을 부착하였을 때가 방해판이 없을 때와 비교하여 동력수가 커지기 시작하는 영역임을 알 수 있었다. 또한 교반 레이놀즈수가 300을 넘어 유동 상태에 약간의 난류가 섞이게 되면, 이 같은 흐름 양상의 단절이 해소되어 완전히 혼합되었다.

• 대한의생명과학회지
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• 제19권3호
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• pp.245-253
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• 2013

To provide reference data or guideline for empirical treatment of bloodstream infection, we studied a change pattern in causative microorganisms and antimicrobial susceptibility in a general hospital at Gyeonggi province during five years. We retrospectively reviewed the frequency of causative microorganisms and antimicrobial susceptibility results of 5,782 microorganisms isolated from blood culture in a general hospital during the period from January 2008 to December 2012. The most common pathogens were Escherichia coli (14.7%), Staphylococcus aureus (7.5%), Streptococcus viridans group (4.9%), and Klebsiella pneumoniae (4.1%). The multiple microorganisms were isolated in 4.3% of bloodstream infection patients. The average contamination rate of blood culture during five years was 3.0%. Methicillin-resistant S. aureus (MRSA), vancomycin-resistant Enterococcus faecium (VRE), and penicillin-resistant Streptococcus pneumoniae were isolated at 62%, 27% and 11%, respectively. Cefotaxime-resistant E. coli and K. pneumoniae was 20% and 18%, respectively. Imipenem-resistant Pseudomonas aeruginosa (IRPA) and Acinetobacter baumannii (IRAB) was 25% and 66%, respectively. E. coli and S. aureus were most common pathogens isolated from blood culture for five years. The increase of multidrug-resistant microorganisms, such as MRSA, VRE, ESBL, IRPA and IRAB, requires more strict control of antibiotics and causes the need of the more updated guideline for the treatment of blood stream infection.

• Journal of Nutrition and Health
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• 제9권2호
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• pp.98-101
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• 1976

The essential amino acids composition of Green algae (chlorella ellipsoidea) that was isolated near the suwon was compared with that of soy flour and the provisional pattern of amino acids of reference protein of FAO and found that the limit amino acid was methionine. The rest of essential amino acids were distributed widely and abunduntly. While soy flour was defficient in methionine and valine on comparing with the provisional pattern. Slightly more content of methionine was recognized in chlorella protein than that of spirulina maxima which was reported to be eaten in the Lake Chard region of Africa and Mexico as a daily food. It was concluded that good quality protein is able to be synthesised from the wild alage if a qualified alage media was given to them.

• 생명과학회지
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• 제11권3호
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• pp.266-272
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• 2001

A total of 79 Salmonella spp. were isolated from Pusan area in 2000. The serotypes of 79 Salmonella isolates were classified as 42 strains of S. typhi(53.1%), 24 strains of S. enteritidis(30.4%), 9 strains of S. montevideo(11.4%), 2 strains of S. typhimurium(2.5%), 1 strain of S. infantis(1.3%) and 1 strain of S. indiana(1.3%) strains(16.5%) of Salmonella sp. were isolated at May July, respectively. The isolates of S. typhi were sensitive to most sntibiotics except streptomycin. All isolates of S. typhi were especially sensitive to tobramycin, gentamicin, colistin, kanamycin, samikacin, sulfamethozazole/ trimethoprim, cefriaxone, ceftazdime, cifrofloxacin, cefoxitin and cefotaxime. Isolates of S. enteritidis wer presented higher resistance than isolates of S. typhi. Twenty-four strains of S. enteritidis were sensitive to kanamycin, amikacin cifrofloxacin, cefoxitin and cefotaxime, however 13 strains(54.2%) of S. enteritidis were resistant to carbenicillin, ampicillin and ticarcillin. Nine strains of S. montevideo were sensitive to most antibiotics except carbenicillin and streptomycin. Each 1 stain of S. indiana and S. infantis was sensitive to most antibiotics used in this study except streptomycin. Three kinds of resistant pattern (CB, SM, TE, AM, TC). In the case of S. enteritidis isolates, 9 kinds resistant pattern were detected. Most frequent resistant pattern of S. enteritidis isolates was CB, AM, TC type(16.7%)