• 한국임상수의학회지
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• 제33권2호
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• pp.142-144
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• 2016

We report a case of Escherichia coli septicemia in a 6-year-old male bottlenose dolphin (Tursiops truncatus). Gross lesions included turbid reddish yellow ascites, fibrous adhesions of rectum and peritoneum, multifocal mucosal ulcers of rectum, and systemic petechiae. Multifocal necrosis with bacterial colonies was observed histologically in mucosal membrane of rectum and anus, and also in caudal mesenteric lymph node, inguinal lymph node, tracheobronchial lymph node, tonsil, spleen, liver, and lung. E. coli was isolated in pure culture from multiple organs including blood, spleen, mesenteric lymph node, liver, lung, and ascites. The E. coli was serotype O25. This case was diagnosed as a septicemia caused by E. coli serotype O25 associated with proctitis.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제13권4호
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• pp.2223-2242
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• 2019

Distance Vector-Hop (DV-Hop) algorithm is widely used in node localization. It often suffers the wormhole attack. The current researches focus on Double-Wormhole-Node-Link (DWNL) and have limited attention to Multi-Wormhole-Node-Link (MWNL). In this paper, we propose a security DV-Hop algorithm (AMLDV-Hop) to resist MWNL. Firstly, the algorithm establishes the Neighbor List (NL) in initialization phase. It uses the NL to find the suspect beacon nodes and then find the actually attacked beacon nodes by calculating the distances to other beacon nodes. The attacked beacon nodes generate and broadcast the conflict sets to distinguish the different wormhole areas. The unknown nodes take the marked beacon nodes as references and mark themselves with different numbers in the first-round marking. If the unknown nodes fail to mark themselves, they will take the marked unknown nodes as references to mark themselves in the second-round marking. The unknown nodes that still fail to be marked are semi-isolated. The results indicate that the localization error of proposed AMLDV-Hop algorithm has 112.3%, 10.2%, 41.7%, 6.9% reduction compared to the attacked DV-Hop algorithm, the Label-based DV-Hop (LBDV-Hop), the Secure Neighbor Discovery Based DV-Hop (NDDV-Hop), and the Against Wormhole DV-Hop (AWDV-Hop) algorithm.

• 한국컴퓨터정보학회논문지
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• 제24권5호
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• pp.1-9
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• 2019

In this paper, we propose the algorithms which determine 1) the efficient anchor-node visiting route of mobile sink in terms of energy supply and 2) the efficient energy amount to be charged to each anchor node, by using the information of each anchor node and the mobile sink. Wireless sensor networks (WSNs) using mobile sinks can be deployed in more challenging environments such as those that are isolated or dangerous, and can also achieve a balanced energy consumption among sensors which leads to prolong the network lifetime. Most mobile sinks visit only some anchor nodes which store the data collected by the nearby sensor nodes because of their limited energy. The problem of these schemes is that the lifetime of the anchor nodes can be shorten due to the increased energy consumption, which rapidly reduces the overall lifetime of WSN. This study utilizes a mobile sink capable of wireless power transmission to solve this problem, so a mobile sink can gather data from anchor nodes while charging energy to them. Through the performance verification, it is confirmed that the number of blackout nodes and the amount of collected data are greatly improved regardless of the size of the network.

• BMB Reports
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• 제29권2호
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• pp.127-132
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• 1996

Germinal centers (GCs) are formed in peripheral lymphoid tissues in response to protein antigens. In order to see if immunoglobulin isotype switching takes place in GC B-cells, we isolated GC B-cells (PNA positive cells) from mouse popliteal lymph nodes by a flow cytometer after the staining of lymph node cells with PNA-FITC and anti-B220-PE, and determined the expression of ${\gamma}1$ germline transcript and ${\gamma}1$ mRNA by RT-PCR. ${\gamma}1$ germline transcript and ${\gamma}1$ mRNA were amplified specifically in cDNAs from hybridoma expressing IgG1 or splenocytes stimulated LPS plus IL-4. Germinal center B-cells formed in popliteal lymph nodes of mice immunized with chicken ovalbumin were isolated 7 days after immunization. We sorted GC B-cells five times. Immunoglobulin ${\gamma}1$ germline transcripts were expressed in germinal center B-cells in three out of five sorts whereas two out of five sorts did not express ${\gamma}1$ germline transcripts in GC B-cells. The contents of GC B-cells ranged from 5 to 7% of total lymph node cells in most flow cytometric analyses but those of two sorted cells which did not express ${\gamma}1$ germline transcripts were out of normal range. These results imply that isotype switching of immunoglobulins may take place in GCs.

• 대한수의학회지
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• 제22권1호
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• pp.31-36
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• 1982

This study was taken to clarify the clinical signs and macroscopical lesions of pigs naturally infected with hog cholera. The clinical and macroscopical observation on the natural cases of hog cholera and experimental cases inoculated with ALD Virus and isolated virus strains were carried out. The results obtained are as follow; In clinical inspection of the natural cases, diarrhea (73.1%) blotching of ear (50.0%), staggering (42.3%), erythema of skin (40.0%), constipation (38.5%), conjunctivitis (32.7%) and dyspnea (30.8%) were observed. Dyspnea, constipation and erythema of skin were observed mainly in the experimental cases, however, staggering and conjunctivitis in pigs infected with ALD virus were found and convulsion and hemorrhage of skin of pigs infected with isolated virus were seen, respectively. The gross lesions of natural cases were hemorrhage of lymph node (82.5%), enteritis and hemorrhage of large intestine (65.0%), splenic infarction (57.5%), pneumonia (55.0%), gastritis and hemorrhage (52.5%), cardiac hemorrhage (40.0%) and renal petechiation (37.5%), while in the experimental cases, hemorrhage of lymph node, pneumonia, gastritis and hemorrhage, enteritis and hemorrhage of laryge intestine and splenic infarction were seen mainly.

• Korean Journal of Radiology
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• 제21권5호
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• pp.605-613
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• 2020

Objective: To determine the malignancy risk of isolated macrocalcifications (a calcified nodule with complete posterior acoustic shadowing) detected on ultrasonography (US) and to evaluate the postoperative American Thyroid Association (ATA) risk stratification of malignant tumors manifesting as isolated macrocalcifications. Materials and Methods: A total of 3852 thyroid nodules (≥ 1 cm) of 3061 consecutive patients who had undergone biopsy between January 2011 and June 2018 were included in this study. We assessed the prevalence, malignancy rate, and size distribution of isolated macrocalcifications and evaluated the histopathologic features and postoperative ATA risk stratification of malignant tumors manifesting as isolated macrocalcifications. Results: Isolated macrocalcifications were found in 38 (1.2%) of the 3061 patients. Final diagnosis was established in 30 (78.9%) nodules; seven malignant tumors were diagnosed as papillary thyroid carcinomas (PTCs). The malignancy rate of the isolated macrocalcifications was 23.3% in the 30 nodules with final diagnoses and 18.4% in all nodules. Among the six surgically-treated malignant tumors, five (83.3%) had an extrathyroidal extension (ETE) (minor ETE 1, gross ETE 4), and two (33.3%) had macroscopic lymph node metastasis. Four (66.7%) malignant tumors were categorized as high-risk tumors, one as an intermediate-risk tumor, and one as a low-risk tumor using the ATA risk stratification. Histopathologically, out of the six malignant tumors, ossifications were noted in four (66.7%) and predominant calcifications in two (33.3%). Conclusion: The US pattern of isolated macrocalcifications (≥ 1 cm) showed an intermediate malignancy risk (at least 18.4%). All malignant tumors were PTCs, and most showed an aggressive behavior and a high or intermediate postoperative ATA risk.

• Journal of Communications and Networks
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• 제1권2호
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• pp.89-98
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• 1999

In a synchronization (sync) $network^1$containing N nodes, it is shown (Theorem 1c) that an arbitrarily connected sync network & is the union of a countable set of isolated connecting sync networks${&_i,i= 1,2,.., L}, I.E., & = \bigcup_{I=1}^L&_i$ It is shown(Theorem 2e) that aconnecting sync network is the union of a set of disjoint irreducible subnetworks having one or more nodes. It is further shown(Theorem 3a) that there exists at least one closed irreducible subnetwork in $&_i$. It is further demonstrated that a con-necting sync network is the union of both a master group and a slave group of nodes. The master group is the union of closed irreducible subnetworks in $&_i$. The slave group is the union of non-colsed irre-ducible subnetworks in $&_i$. The relationships between master-slave(MS), mutual synchronous (MUS) and hierarchical MS/MUS ent-works are clearly manifested [1]. Additionally, Theorem 5 shows that each node in the slave group is accessible by at least on node in the master group. This allows one to conclude that the synchro-nization information avilable in the master group can be reliably transported to each node in the slave group. Counting and combinatorial arguments are used to develop a recursive algorithm which counts the number $A_N$ of arbitrarily connected sync network architectures in an N-nodal sync network and the number $C_N$ of isolated connecting sync network in &. EXamples for N=2,3,4,5 and 6 are provided. Finally, network examples are presented which illustrate the results offered by the theorems. The notation used and symbol definitions are listed in Appendix A.

• 한국동물위생학회지
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• 제30권3호
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• pp.375-384
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• 2007

This study was conducted to investigate the characteristics of brucellosis in Korean native cattle in a farm where bovine brucellosis was confirmed 3 times from September 2006 to March 2007. Of 74 bulls serum samples examined, 21 (28.4%) were positive by Rose-Bengal test (RBT) and Standard tube agglutination test (STAT). In the isolation test from seropositive bulls, B abortus was isolated and identified from 2 specimens (testis, intestinal lymph node) among 6 kinds of specimens including blood, urine, feces and soil. Isolation rate of intestinal lymph node and testis was 25% (3/12 cases) and 16.7% (2/12), respectively. B abortus was also isolated from calves below 12 months old, i.e., 1 isolate (25.0%) was confirmed from testis, 4 (40.0%) from supra-mammary lymph nodes and 1 (25.0%) from intestinal lymph node. All isolates had Brucella specific 16s r-RNA with 905-bp band detected by PCR assay. For the more effective control of bovine brucellosis in korea, this paper would like to suggest that all of bulls and calves should be included in the screening tests.

• 대한수의학회지
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• 제43권4호
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• pp.667-675
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• 2003

The safety of Brucella abortus strain RB51(SRB51) was investigated in dairy cows and Korean native cattle of 4~7th month of gestation. From experimentally inoculated cattle, 18 of 25 (72.0%) dairy cows, and 3 of 10 (30.0%) Korean native cattle were aborted or delivered premature fetus. There were no significant differences in the incidence of abortion depending on the inoculation route (intramuscular and subcutaneous) and dosages ($1{\times}10^9$, $2.8{\times}10^9$, and $4.0{\times}10^9$ CFU). The antibodies to the SRB51 were measured by a dot blot enzyme-linked immunosorbent assay. The highest titers to SRB51 were detected between 5~7 weeks after inoculation and the specitic antibody could be detected up to 28 weeks after inoculation. The SRB51 was isolated from amnio-allantoic fluid, bronchial lymph node, mammary gland, and supramammary lymph node in 5 of 25 dairy cows during 4 weeks after either abortion or delivery. Although SRB51 was isolated from 4 of 24 aborted fetus or normally delivered calves at parturition time, it was not isolated during 4 weeks afterward. Eleven of twentyfive dairy cows showed the endometritis and/or necrosis until 6 weeks after delivery, no lesions were seen at 8 weeks after delivery and uterus from control dairy cows. The results of present study revealed that SRB51 might induce the clinical signs of brucellosis in the pregnant cattle at 4~7th month of gestation.

• Journal of Life Science
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• 제12권1호
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• pp.22-26
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• 2002

CS21 lymphoma cells that preferentially metastasize to lymph nodes after s.c. inoculation into BALB/c mice were grown in vitro in the presence of CA12 stromal cells isolated from lymph nodes. To obtain fundamental data on identification and characterization of the soluble factor(s) produced by CA12 stromal cells, we investigated the biological profile of the conditioned medium produced by CAl2 stromal cells. CAl2 conditioned medium has no affinity with Con A. CAl2 conditioned medium is associated with the proliferation of splenic T- and thymic T-cells with-out adding mitogen, although the conditioned medium cannot induce the differentiation of thymocytes. Additionally, we showed that H-7, not HA-1004 inhibits CS21 cell proliferation. These results suggest that CAl2 conditioned medium has a specific soluble factor(s) produced by lymph node stromal cells.