• 제목/요약/키워드: Isolated cultivation

검색결과 625건 처리시간 0.027초

Probiotics에 의한 해수양식어의 성장 촉진 및 항균효과 (Antibacterial Activity and Growth Promotion in Aquacultured Fish by Probiotics)

  • 백남수;임유범;김영만
    • 한국미생물·생명공학회지
    • /
    • 제29권1호
    • /
    • pp.56-61
    • /
    • 2001
  • For the development of probiotics in aquaculture of marine organisms, three strains having psychrophilic and salt tolerant characteristics were isolated from Kimchi. Amng the isolated strains. MG19, MG89 and MG208 were identified as Lactobacillus brevis, Enterococcus faecium and Lactobacillus plantarum, respectively. The neutralized culture broth of isolated strains were tested in order to evaluate the antibactrial activity, 조초 showed high antibacterial activity against Edwardsiella tarda, Vibrio cholerae andPseudomonas fluorescens. In mixed culture of pathogens and isolated strain,pathogens were signifi-cantly inhibited after 2 days of cultivation but the isolated strains showed normal growth. When the Edwardsiella tarda was cultured with three isolated strains, its growth was completely inhibited after 254 hours of cultivation. The effect of isolated three strains as probiotics was investigated based on the changes in body weight of aquacultured flounder. After 50 days feeding trial, it was found that the mean body weight gain of the tested group fed freeze-dried isolated cells was significantly greater than that of the control group. These results suggest that these isolated strains can play an important role as probiotics in aquaculture.

  • PDF

농산물 생산 환경에서 분리된 Staphylococcus aureus의 항생제 내성 및 독소 유전자 확인 (Identification of Toxin Gene and Antibiotic Resistance of Staphylococcus Aureus Isolated from Agricultural Product Cultivation Environments)

  • 박수희;김정숙;김경열;정덕화;심원보
    • 한국환경보건학회지
    • /
    • 제39권5호
    • /
    • pp.465-473
    • /
    • 2013
  • Objectives: This study was undertaken to analyze Staphylococcus aureus from cultivation environments for agricultural products and to confirm antibiotic resistance and enterotoxin genes for the isolated S. aureus. Methods: A total of 648 samples were collected from apple, peach, ginseng and balloon flower farms. S. aureus was isolated from soil, agricultural water, personal hygiene elements (hands, gloves and clothes) and work utensils (boxes). Results: S. aureus was detected in a total of 25 samples and 72 strains were isolated. The resistance rate of the isolated S. aureus strains was confirmed at 33.3%, with 24 resistant strains among the total of 72. Fourteen different patterns types were found, and three pattern types (NV, OX, VA) were confirmed most frequently. As result of the detection of enterotoxin gene type, four gene types (sea: 1, sed: 4, seg: all isolated S. aureus, sei: all isolated S. aureus) were analyzed among a total of nine types. Conclusions: This study demonstrates that personal hygiene techniques should be properly managed, such as washing and sterilization before or after work, because agricultural contamination by S. aureus frequently developed through improper management.

잔디 토양전염성병원진균에 대한 길항미생물의 분리 및 길항효과 (Isolation of Antibiotic-producing Microorganisms Antagonistic to Soilborne Pathogenic Fungi of Bentgrass and Their Antifungal Activity)

  • 이용세;전하준;이창호;송치현
    • 한국유기농업학회지
    • /
    • 제6권1호
    • /
    • pp.133-149
    • /
    • 1997
  • Recently, the importance of management and cultivation of grasses has been increased in Korea. Among these cultural practices, the appropriate control of diseases is considered more important than other cultivation techniques such as fertilization and irrigation. The damages of brown patch and large patch caused by Rhizoctonia spp. and Pythium blight caused by Pythium spp. are serious in the major cultivation area of turfgrass in Korea. Since these diseases are difficult to control by agrochemicals, the damages are very serious if these are occured. The periodic spray of agrochemicals, to protect and control these diseases could make some problems of toxicity and environmental pollution as well as rising of non-target diseases. Therefore, the biological methods to control diseases have been required to decrease problems resulted from overuse of agrochemicals, to conserve natural ecosystem, and to control effectively diseases of grasses in the long period. The number of studies about biological control using antagonistic microorganisms have been increased for last half century. However, the application of biological control method has been very limited. In this study, thirteen isolates of R. cerealis, 8 isolates of R. solani and 3 isolates of Phthyn spp. have been isolated from diseased turfgrass in golf course and grass-culture area that have patch and wilting symptoms of zoysia grass and creeping bentgrass. Isolation frequency of R. cerealis and R. solani was high in especially zoysiagrass, while Pythym spp. was isolated from bent grass at low frequency but showed high pathogenicity. Totally, 205 isolates of soil microorganisms were isolated in this study as primary antagonistic microorganism by Herr's triple agar layer plate and dual culture method using rhizosphere of grasses, soil of crop field as the source of antagonistic microorganisms. Among the 205 isolates, 23 isolates were actinomycetes and 182 isolates were bacteria. All of the actinomycetes were isolated by Herr's method. Antagonistic effect of primary isolated microorganisms was tested for in vitro mycelial growth inhibition against pathogenic fungi isolated from grasses and for inhibition of disease occurrence in 24 well tissue culture plate and pot experiment. Then, four isolated of bacteria which are BG23, BG74, BG136 and BG171 were selected as antagonistic microorganisms against soil-born pathogenic fungi of bentgrass.

  • PDF

Bacillus subtilis가 Corn ( Zea mays L. ) , White Clover ( Trifolium repens L. ) 및 Tall Fescue ( Festuca arundinacea Schreb. ) 유식물의 생육에 미치는 영향 (Effecets of Bacillus subtilis on Growth of Seedings in Corn ( Zea mays L. ) , White Clover ( Trifolium repens L. ) and Tall Fescue ( Festuca arundinacea Schreb. ))

  • Park, Ki-Chun;Chang Youn;Kim, Dong-Am
    • 한국초지조사료학회지
    • /
    • 제18권3호
    • /
    • pp.195-204
    • /
    • 1998
  • This study was designed to investigate the effects of antagonistic microorganism, Bacillus subtilis, on the growth of forage seedlings in repeated cultivation soils and unrepeated cultivation soils. The field experiment was wnducted in pots in a vinyl house using repeated and unrepeated cultivation soils. Forage types were 'Suwon 19' wrn(Zea mqs L.), 'Califbmia' white clover(Tr~oIium repens L.) and 'Fawn' tall fescue (Festuca arundianacea Schreb.). Samples of white clover and tall fescue were taken h m each pot at 36 days after seeding. Samples of wm were examined at 50 days after seeding. The most active antagonistic bacterium was isolated h m forage rhizosphere soil, and selected by reference to it's antagonistic ability on the growth of pathogenic fungi, Rhizoctonia solmi and Fusarium oxyspomm, and it was identified as Bacillus subtilis. This strain strongly suppressed the growth of fungal pathogens among isolated rhizobacteria. The dry weight of forage shoots and roots cultivated in unrepeated cultivation soils was higher than that cultivated in repeated cultivation soils. The dry weight of forage was positively affected by the inoculation of the antagonistic bacterium, Bacillus subtilis, in both repeated cultivation soils and unrepeated cultivation soils. In conclusion, the growth of forage was more affected by the inoculation of the antagonistic bacterium in unrepeated cultivation soils than that in repeated cultivation soils, and bacterization of forage with B. subtilis resulted in an inrreased yield.

  • PDF

우리나라 식물검역 격리재배 시스템과 2005-2012년 실적보고 (Plant quarantine isolated cultivation system in Korea and results of recorded in 2005-2012)

  • 이시원;박정안;이오미;신용길
    • 농업과학연구
    • /
    • 제40권4호
    • /
    • pp.281-287
    • /
    • 2013
  • 우리나라의 격리재배는 102속, 약 250여종의 식물에 대해 실시하고 있으며, 직접경검법, 배양법, 선택배지, 생리생화학, ELISA 및 PCR 검사방법을 사용한다. 2005-2012년, 우리나라에서 수행된 격리재배는 총 8,307건이며 이중 구근류가 5,165건(62.2%)로 가장 많았고, 묘목류가2,119건(25.0%), 종자 796건(9.6%), 삽수 150건(1.8%), 접수 70건(0.8%) 및 기타 7건(0.1%) 이었다. 불합격 사례는 총 413건으로 약 4.97%였고, 발견된 병의 종류는 총 47종으로 나타났다. 종류별로는 바이러스가 27종으로 가장 많은 수를 차지했으며, 곰팡이 16종, 바이로이드 1종, Chromalveolata 1종 및 기타 2종으로 나타났다. 가장 많은 검역건을 올린 병원체는 Arabis mosaic virus (77건), Tobacco rattle virus (70건), Lily symptomless virus (46건), Penicillium expansum (46건)이다.

Optimization of the Bacteriophage Cocktail for the Prevention of Brown Blotch Disease Caused by Pseudomonas tolaasii

  • Yun, Yeong-Bae;Um, Yurry;Kim, Young-Kee
    • The Plant Pathology Journal
    • /
    • 제38권5호
    • /
    • pp.472-481
    • /
    • 2022
  • Brown blotch disease, caused by Pseudomonas tolaasii, is one of the most serious diseases in mushroom cultivation, and its control remains an important issue. This study isolated and evaluated pathogen-specific bacteriophages for the biological control of the disease. In previous studies, 23 varieties of P. tolaasii were isolated from infected mushrooms with disease symptoms and classified into three subtypes, Ptα, Ptβ, and Ptγ, based on their 16S rRNA gene sequences analysis and pathogenic characters. In this study, 42 virulent bacteriophages were isolated against these pathogens and tested for their host range. Some phages could lyse more than two pathogens only within the corresponding subtype, and no phage exhibited a wide host range across different pathogen subtypes. To eliminate all pathogens of the Ptα, Ptβ, and Ptγ subtype, corresponding phages of one, six, and one strains were required, respectively. These phages were able to suppress the disease completely, as confirmed by the field-scale on-farm cultivation experiments. These results suggested that a cocktail of these eight phages is sufficient to control the disease induced by all 23 P. tolaasii pathogens. Additionally, the antibacterial effect of this phage cocktail persisted in the second cycle of mushroom growth on the cultivation bed.

Cultivation of Lactobacillus crispatus KLB46 Isolated from Human Vagina

  • Chang, Chung-Eun;Kim, Seung-Cheol;So, Jae-Seong;Yun, Hyun-Shik
    • Biotechnology and Bioprocess Engineering:BBE
    • /
    • 제6권2호
    • /
    • pp.128-132
    • /
    • 2001
  • Bacterial vaginosis can be treated by restoring the normal vaginal flora using lactobacilli. Lactobacillus crispatus KLB46 that was isolated from the human vagina has a string antimicrobial activity and was grown in a batch and in a continuous fermentor. During batch cultivation, the maximum specific growth rate of L. crispatus KLB 46 was 0.63h(sup)-1 and the highest viable cell count (1.9$\times$10(sup)9 CFU/mL) was obtained at pH 5.5. L. crispatus KLB 46 did not grow well at either pH 3.5 or 7.5. During continuous cultivation, the highest viable cell count (1.53$\times$10(sup)9 CFU/mL) was obtained at a dilution rate of 0.32h(sup)-1, and was 7.33$\times$10(sup)11 CFU L(sup)-1 h(sup)-1, that is approximately 5 times higher than that obtained from batch culture.

  • PDF

고추 및 토마토 친환경 및 관행재배지에서 분리한 인체 유해세균의 항생제 저항성 평가 (Evaluation of Antibiotics Resistance for Human-harmful Bacteria Isolated from Eco-friendly and Practical Cultivation Farms of Hot Pepper and Tomato)

  • 이성희;도지원;김성겸;오광교;박재호
    • 한국유기농업학회지
    • /
    • 제31권4호
    • /
    • pp.381-394
    • /
    • 2023
  • This study was conducted to monitor the antibiotics resistance of human-harmful bacteria isolated in the agricultural environment for hot peppers (Capsicum annuum) and tomato (Lycopersicon esculentum). As a result, we isolated 120 bacterial species (34 on fruits, 48 in soil, 21 in water, and 17 in manure), identified them with the 16S rRNA sequence, analyzed minimum inhibitory concentration (MIC) for 26 antibiotics using Sensititre ARIS Hi-Q system and then evaluated whether each bacterial genus acquired resistance for the tested antibiotics or not, according to the CLSI criteria. From difference in MIC between eco-friendly (EFM) and practical (PFM) cultivation farms, Klebsiella spp. isolated from EFM was resistant to ampicillin (AMP) and nalidixic acid (NAL), and that isolated from PFM was resistant to streptomycin (STR) and tetracycline (TET). Enterobacter spp. isolated from EFM was resistant to AMP and azithromycin (AZI), and that isolated from PFM was resistant to AMP, AZI, and STR. Meanwhile, Pseudomonas spp. isolated from EFM and PFM were all resistant to AMP, AZI, cefotaxime (FOT), cefoxitin (FOX), ceftriaxone (AXO), CHL, NAL, and STR. Staphylococcus spp. isolated from EFM and PFM were resistant to gentamycin (GEN), STR, and kanamycin (KAN), and in particular, that from EFM showed resistance for erythromycin (ERY). In conclusion, our study suggested that EFM lead STR antibiotics resistance for human-harmful bacteria to decrease, because only the bacteria isolated from hot pepper and tomato crop with PFM have showed resistance against STR antibiotics, regardless of bacterial genus.

멜론의 수량과 품질에 미치는 근권제한의 효과 (Effect of Root Zone Restriction on Yield and Quality of Muskmelon)

  • 권준국;이재한;엄영철;최영하;강광윤;박동금
    • 생물환경조절학회지
    • /
    • 제8권1호
    • /
    • pp.36-41
    • /
    • 1999
  • 본 연구는 토양근권제한이 멜론의 수량과 품질에 미치는 영향을 구명하고자, 격리상, 간이근권제한시트 및 관행 토양재배의 3가지 재배방법을 적용하여 멜론을 4월19일 부터 7월 17일까지 재배하였다. 지온은 격리상재배가 토양재배와 근권제한시트 재배보다 다소 높게 유지되었고 멜론의 생육은 토양과 근권제한시트재배에 비해 격리상 재배에서 빨랐다. 과실크기는 토양, 격리상 및 근권제한시트재배 순이었으나 당도 및 네트형성은 격리상재배가 토양재배에 비해 우수하였다. 수확기 경엽의 시들음주율은 토양재배 26.7%, 근권제한시트재배 25% 발생한 데 비해 격리상재배는 3.3%로 가장 낮았다 따라서 상품과 수량은토양재배에 비해 격리상재배에서 많았다.

  • PDF

원목 및 톱밥배지 버섯 재배사 내 실내 공기서 분리한 미기록 진균 보고 (Unrecorded Fungal Species Isolated from Indoor Air in the Log Bed- and Sawdust Media-based Mushroom Cultivation Houses)

  • 안금란;김지은;김준영;김성환
    • 한국균학회지
    • /
    • 제46권4호
    • /
    • pp.495-503
    • /
    • 2018
  • 2016년 4월부터 6월까지 표고 원목재배 임가 4곳과 표고 톱밥재배 임가 1곳의 재배사 내 실내 공기에서 진균을 포집 및 분리 동정하였다. 총 6개의 국내 미기록 진균(Cenangium acuum, Periconia macrospinosa, Neopestalotiopsissurinamensis, Metarhiziummarquandii, Trichoderma petersenii, Trichoderma paratroviride)을 분리하였으며 이들 균류에 대하여 형태학적 특성 및 internal transcribed spacer rDNA region, large subunitregion, ${\beta}-tubulin$ 또는 translation elongation factor $1{\alpha}$유전자 염기서열에 기반하여 계통학적 분석 결과를 기술하였다.