• The Journal of Internal Korean Medicine
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• v.11 no.2
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• pp.68-79
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• 1990

This study was carried out to investigate the effect of Heapyoyangjintang and Haepyoejintang extract on the contractile force of the isolated guinea pig trachea smooth muscle and elucidate its mechanism. The results were obtained as follows : 1. The isolated trachea smooth muscle was suspended in the organ bath with oxygenated kreb's Henseleit bicarbonate buffer solution at $37^{\circ}C$, and the developed tension by the drug was recorded with Isometric transducer(nacro F-60). The resting tension was approximately 0.5g. 2. The trachea smooth muscle of the isolated guinea pig was significantly relaxed by the administration of Haepyoyangjintang and Haepyoejintang extract. 3. The contractile response of the trachea smooth muscle of the isolated guinea pig to histamine$10^4M$ Was significantly inhibited by Heapyoyangjintang and Haepyoejintang extract. 4. The contractile response of the trachea smooth muscle of the isolated guinea pig to acetylcholine$10^4M$ was considerably inhibited by Haepyoyangjintang and Haepyoejintang extract. 5. The contractile response of the trachea smooth muscle of the isolated guinea pig to 5-hydorxytrtrypptamine $10^4M$ was considerably inhibited by Haepyoyangjintang and haepyoejintang extract.

• Korean Journal of Soil Science and Fertilizer
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• v.45 no.5
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• pp.798-804
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• 2012

Although siderophores are induced primarily in response to iron deficiency, soil and other ecological factors can affect on this process. This study was to evaluate the production of siderophores by different fungal species isolated from heavy metal contaminated and uncontaminated soils. More than thirty fungal strains were isolated from heavy metal contaminated and rhizosphere uncontaminated soils. Chrome azurol sulfonate (CAS) was used for both quantitative and qualitative evaluation of siderophores production. No significant correlations were observed between the tested variables such as ultraviolet (UV) irradiation method and CAS-agar plate and heavy metal concentration in both soils. The production of siderophores in rhizosphere fungi was higher than those isolated from the contaminated soil; however, the difference was not significant. The siderophore production (%) by fungi isolated from heavy metal contaminated soil using UV irradiation method was positively correlated with the qualitative values using CAS-plate method (P<0.05). Pearson correlation test indicated a positive correlation between the quantitative and qualitative methods of detection for fungi isolated from rhizosphere and also those isolated from heavy metal contaminated soil.

• The Journal of Internal Korean Medicine
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• v.13 no.1
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• pp.70-79
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• 1992

This study was carried out to investigate the effect of Yeemosan extract on the contractile force of the isolated guinea pig trachea smooth muscle and elucidate its mechanism. The results were obtained as follows: 1. The contractile response of the trachea smooth muscle of isolated guinea pig to acetylcholine was significantly inhibited by Yeemosan. 2. The contractile response of the trachea smooth muscle of isolated guinea pig pretreated propranolol was significantly inhibited by Yeemosan. 3. Effects of Yeemosan extract on the contractile response of the isolated guineapig trachea smooth muscle pretreated methylene blue was not significant. 4. The contractile response of the trachea smooth muscle of isolated guinea pig to prostaglandin $F2{\alpha)$ was significantly inhibited by Yeemosan. 5. Effects of prostaglandin $F2{\alpha)$ on the contractile response of the isolated guinea pig trachea smooth muscle pretreated Yeemosan was not significant. According to the above results. it was suggested that the contractile response mechanism of the guinea pig trachea smooth muscle to Yeemosan was related to sympathetic nervous system receptor and other mechanism should have further study.

• The Journal of Internal Korean Medicine
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• v.13 no.1
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• pp.34-45
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• 1992

This study was carried out to investigate the effect of Shihogigiltang extract on the contractile force of the isolated guinea pig trachea smooth muscle and elucidate its mechanism. The results were obtained as follows: 1. The isolated trachea smooth muscle was suspended in the organ bath with oxygenated Kreb's Henseleit bicarbonate buffer solution at $37^{\circ}C$, and the developed tension by the drug was recorded with isometric transducer(Nacro F-60). The resting tension was approximately 0.5g. 2. The trachea smooth muscle of the isoiated guinea pig was significantly relaxed by the administration of Shihogigiltang extract. 3. ShihogigiItang significantly inhibited the contractile response of histamine 10-4 M is isolated guinea pig trachea smooth muscle. 4. The contractile response of the trachea smooth muscle of the isolated guinea pig by acetylcholine 10-4 M was significantly inhibited by Shihogigiltang extract. 5. The contractile response of the trachea smooth muscle of the isolated guinea pig by 5-hydorxytryptamine 10-4 M was significantly inhibited by Shihngigiltang extract. 6. The contractile response of the trachea smooth muscle of the isolated guinea pig by prostaglandin $F2\;{\alpha}$ 10-7 M was significantly inhibited by Shihngigiltang extract.

• Korean Journal of Plant Resources
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• v.6 no.1
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• pp.13-23
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• 1993

To data, many types of compounds having antineoplastic activity have been isolated from higher plants, that is, alkalodids, terpenes, lignans, steroids and so on. Some of ther were isolated from Indonesian plants, Curcuma xanthorrhiza and Eurycoma longifolia. Bisaborane type compounds were compounds were isolated as antimeoplastic compounds againest Sarcoma 180A from C. xanthorrhiza, and quassinoids and euryrene type triterpenes from triterpenes from El longifolia. Casearines, a kind of diterpene, had been isolated as cytotxic components from Casearia sylvestris distributed in South America. RA series Cyclic hexapeptides isolated from Rubia akane and R. cordifolia also have strong antineoplastic activity against various types of tumors. Till now, 16 kinds of RA series compounds were isolated and named as RA-I~XVI. Moreover, monoglucoside of RA-V newly isolated from same plant. Many kinds of derivatives including natural RA compounds were tested for QSAR, and one of them, RA-VII was screened up as a most suitable substance as an antitumor agent. RA-VII(=RA 700) has strong cytotoxic activity against KB cells, P388 lymphocytic leukemia and MM2 mammary carcinoma cells. In some solution, three conformers of RA-VII were observed by NMR. It was discussed the relationship between conformation and activity. Total synthesis was already completed, but there is left room for improvement. Phase I clinical trials for RA-VII has been finished, then Phase II trials will be started before long.

• Korean Journal of Veterinary Research
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• v.34 no.4
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• pp.727-734
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• 1994

To elucidate the effect of calcitonin gene-related peptide(CGRP), vasoactive intestinal peptide(VIP) and substance P was investigated with perivascular nerve stimulation and treatment of peptides from polygraph in the isolated renal artery of rabbit. 1. The neurogenic contraction induced by perivascular nerve stimulation was the frequency-dependent manner(264 Hz) in the isolated renal artery of rabbit. 2. CGRP and VIP caused the relaxation on the precontraction with noradrenaline($10{\mu}m$) on the presence and absence of endothelium in the isolated renal artery of rabbit. 3. Substance P caused the endothelium-dependent relaxation on the precontraction with noradrenaline($10{\mu}m$) in the isolated renal artery of rabbit. 4. CGRP and VIP inhibited the neurogenic contraction by the perivascular nerve stimulation(0.3 ms, 80 V, 50 Hz, 1 sec) on the absence and presence of endothelium in the isolated renal artery of rabbit. 5. Substance P inhibited on the neurogenic contraction by the perivascular nerve stimulation with the endothelium-dependent in the isolated renal artery of rabbit.

• Geomechanics and Engineering
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• v.20 no.5
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• pp.447-459
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• 2020

Based on the literature statistical method, the paper publication status of the isolated working face and the distribution of the rockburst coal mine were obtained. The numerical simulation method is used to study the stress distribution law of working face under different mining range. In addition, based on the similar material simulation test, the overlying strata failure modes and the deformation characteristics of coal pillars during the mining process of the isolated working face with thick-hard key strata are analyzed. The research shows that, under the influence of the key strata, the overlying strata formation above the isolated working face is a long arm T-type spatial structure. With the mining of the isolated working face, a series of damages occur in the coal pillars, causing the key strata to break and inducing the rockburst occurs. Combined with the mechanism of rockburst induced by the dynamic and static combined load, the source of dynamic and static load on the isolated working face is analyzed, and the rockburst monitoring methods and the prevention and control measures are proposed. Through the above research, the occurrence probability of rockburst can be effectively reduced, which is of great significance for the safe mining of deep coal mines.

• Fisheries and Aquatic Sciences
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• v.18 no.4
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• pp.387-393
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• 2015

Biotyping of Vibrio vulnificus strains isolated from marine environments along the south coast of Korea showed that the majority of the isolates (94.7%) belonged to biotype 1 and the remaining isolates (5.3%) belonged to biotype 2. Analysis of 16S rRNA V. vulnificus strains isolated from marine environments using a multiplex polymerase chain reaction (PCR) revealed that 78.7% were type A and 21.3% were type B. Random amplified polymorphic DNA (RAPD) was used to analyze the genomic differences in V. vulnificus among the biotype 2 strains isolated from marine environments (newly isolated strains group) and reference strains obtained from infected eels (reference strains group). The two groups had distinctly different profiles of the amplicons produced from RAPD. Additionally, biochemical comparison of these strains revealed that all four strains isolated from marine environments differed from the strains isolated from eels in their ability to promote D-mannitol fermentation. Two (NH 1 and NH 2) out of four isolates of biotype 2 from marine environments showed pathogenicity in eels Anguilla japonica in a challenge test. These isolates did not agglutinate with antisera against V. vulnificus NCIMB 2137 (serovar E), ATCC 27562 (non-serovar E), and ATCC 33816 (atypical serovar E).

• Restorative Dentistry and Endodontics
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• v.14 no.1
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• pp.85-96
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• 1989

In the Infected root canals, the majority of the isolated bacteria are either strict anaerobes or microaerophilic organisms. Among thease, Black-pigmented Bacteroides species are the most important and have relation with clinical symptoms. The purpose of this study were to investigate on the 7 different types of bacteria which include Black-pigmented Bacteroides - Black--pigmented Bacteroides, Actinobacillus actinomycetemcomitans, Streptococcus, Capnocytophaga, Eiknella corrodens, Fusobacterium, Actinomyces - and the interrelationship between these 7 bacterical species, and to compare Black-pigmented Bacteroides with the clinical symptoms in infected root canals. The canal contents of 15 necrotized teeth with 8 clinical symptoms were sampled and cultured aerobically and anaerobically for growth in 7 selective agar plates for 7 bacterial species. Black-pigmented Bacteroides and Streptococcus subspecies were identified by biochemical tests. The results were as follows; 1. 70.51% of the bacteria isolated were anaerobes and 29.49% were aerobes. 2. B. loescheii, B. intermedius, B. denticola were isolated in 8 cases, Streptococcus was isolated in 8 cases, Fusobacterium was isolated in 6 cases, Actinomyces was isolated in 5 cases and Eiknella corrodens, Actinobacillus actinomycetemcomitans, Capnocytophaga were not isolated. 3. There were mutual inhibition of growth between Black-pigmented Bacteroides and Streptococcus. (P<0.01) But Actinomyces and Fusobacterium showed mutual aids for growth. (P<0.05) 4. Black-Pigmented Bacteroides was found to be related both to sinus tract formation and to apical sensitivity to palpation.(P < 0.05).

• Korean Journal of Veterinary Service
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• v.32 no.2
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• pp.139-146
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• 2009

Bovine brucellosis is a zoonosis, long incubation period and chronic infectious disease, usually caused by Brucella abortus. This study was carried out to investigate the biotyping and biochemical characterization of B. abortus isolated from 208 farm 871 korean cattle and holstein diagnosed brucellosis by serological positive in Gyeongbuk province during the period from 2002 to 2006. B. abortus was isolated from 124 (14.2%) of 871 cattle, and isolated 110 (13.4%) of 820 Korean cattle and 14 (27.5%) of 51 holstein in breed. The uterus of korean cattle was isolated in 8 (17.8%) of 45 cattle and supramammary lymph none of holstein was isolated 11 (68.8%) of 16 cattle. 101 (12.5%) of 810 serological positive blood samples were isolated B. abortus. The isolation rate of B. abortus was correlated with antibody titers. The biochemical characterization of isolates was non-hemolytic, production of H$_2$S, oxidase-positive, catalase-positive, hydrolyzation of urea and growth of basic fuchsin dye medium. As a result, all of isolates was identified B. abortus bv 1. 124 isolates were susceptible to ampicillin, lincospectin, amikacin, gentamicin, kanamycin, neomycin, streptomycin, tetracycline, ciprofloxacin, norfloxacin and enrofloxacin.