• Applied Biological Chemistry
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• v.41 no.4
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• pp.235-240
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• 1998

The myristoyl-acyl carrier protein (ACP) specific thioesterase from Iris tectorum was purified to a considerable homogeneity and characterized. The enzyme was eluted with a considerable stability by double-gradients using Triton X-100 and low ionic KCl or Na-phosphate through DEAE-52, Octyl-Sepharose, Q-Sepharose, and hydroxyapatite chromatoraphy. SDS-PAGE analysis showed a single band of 39 kDa. The native molecular weight was estimated to be 82 kDa by Sephacryl S-200 chromatography, indicating that the enzyme was a dimer. The thioesterase showed a chain-length specificity to myristoyl-ACP in preference to other-ACPs. The enzyme activity decreased by 1.0 mM myristate to about 27% of the original activity, whereas the remaining activity with decanoate was about 90%. The purified thioesterase was inhibited by myristoyl-CoA more than by myristate, suggesting that the myristoyl-AGP thiolesterase might be controlled by myristic acid and/or a subsequent product myristoyl-CoA. In addition, some biochemical characteristics of the enzyme were described.

• Applied Biological Chemistry
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• v.44 no.1
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• pp.20-23
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• 2001

Several unusual fatty acids including myristic acid (14 : 0) and lauric acid (12 : 0) were investigated in the Iris family and other related plants. Especially the roots of Iris tecorum contained 75.9% myristic acid in total fatty acid contents and that of Iris germanica contained 57% myristic and 15.5% lauric acid (12 : 0) whereas 10.7% lauric acid and 9.5% capric acid (10 : 0) were detected in the roots of iris ensata as compared to the total fatty acid contents. The total fatty acid contents in the seeds of Foeniculi fructus and Torilis japonica was relatively higher 193.3 mg/g dry wt and 128.2 mg/g dry wt, respectively. 64.5% linoleic acid (18 : 2) and 48.9% ${\alpha}-linolenic$ acid (18 : 3) were observed in the seeds and leaves of iris tectorum whereas its lateral roots contained 9.5% caprylic acid (8 : 0) and 8.6% capric acid. The percentage of myristic acid of the total fatty acid in the immature seeds of iris tectorum and Iris germanica was 10.8% and 15.6%, respectively.

• Journal of Plant Biotechnology
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• v.31 no.1
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• pp.19-23
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• 2004

This study was conducted to observe the morphological characteristics and to analyse genetic relationship using RAPD markers in 9 species of Iris. The period of flowering was shown very broad among 9 species of Iris, from April 22 to June 9. The flower stalk was fluctuated from 9.0cm (I. rossii) to 104.0cm (I. ensata). I. ensata has the largest flower size and followed by I. laevigata, I. tectorum, I. sanguinea, I. pseudacorus, I. lactea, I. rossii, I. minutoaurea, I. odaesanensis. Among the 108 amplified bands by PCR with 8 random primers, 107 showed polymorphism and only one showed monomorphism among 9 species of Iris. The average dissimilarity coefficient among 9 species of Iris was 0.252. The range of dissimilarity coefficient was shown as from 0.095 to 0.609. Nine species were divided into three groups. The first group was I. tectorum, and the second group was I. lactea, I. laevigata, I. pseudacorus, I. odaesanensis. I. minutoaurea, I. rossii, I. sanguinea, I. ensata were belong to group three.

• Korean Journal of Plant Taxonomy
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• v.43 no.3
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• pp.227-235
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• 2013

Molecular phylogenetic studies were conducted to evaluate taxonomic identities and relationships among 16 species of the korean genus Iris L. Korean Iris was grouped by five clades. Series Laevigatae, Tripetalae, Laevigatae and Sibiricae was included to Clade I. Series Chinensis, and Easatae was composed to Clade II. Series Chinensis was included to Clade III. Series Chinensis was composed to Clade IV. Series Crossiris, Pumilae and Pardanthopsis was included to Clade V. Iris dichotoma, I. mandshurica and I. tectorum formed one clade, and it was located mostly in the basal group. I. minutiaurea and I. koreana was not formed independent clade, so it is not clear between them about taxonomic identities. Iris tectorum was established taxonomic system by Series Cossiris in Subgenus Crossiris. Series Chinensis (I. odaesanensis, I. minutiaurea, I. koreana, I. rossii var. latifoia, and I. rossii) was distinguished is clear by Series Chinensis (I. odaesanensis, I. minutiaurea and I. koreana) and Series Chinensis (I. rossii var. latifoia and I. rossii). The Genus Iris was divided into four subgenus (Limniris, Crossiris, Iris and Pardanthopsis). We thought that evolved to subgenus Limniris in subgenus Crossiris, iris and Pardanthopsis.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.12 no.5
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• pp.42-58
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• 2009

This study was carried out to survey the vascular plants of the construct-reserved site of ecological forest in Kohang-ri, Sangri-myeon, Yecheon-gun, Gyeongsangbuk-do, Korea. The results are as follows. The vascular plants in this site were recorded as 392 taxa; 85 families, 254 genera, 341 species, 45 varieties and 6 forma. The Korean endemic plants were 5 taxa; Salix hallaisanensis, Clematis trichotoma, Chrysosplenium barbatum, Philadelphus schrenckii and Weigela subsessilis. The rare plants designated by Korea Forest Service were 2 taxa; Iris ensata var. spontanea and Aristolochia contorta. The naturalized plants were 25 taxa; Bromus tectorum, Dactylis glomerata, Panicum dichotomiflorum, Bilderdykia dumetora and so forth. In the results of analysis by floristic region, the specific plants were summarized as 31 taxa; Matteuccia struthiopteris, Oxalis obtriangulata, Betula davurica, Cimicifuga heracleifolia and so forth. The plants with approval for delivering oversea were 4 taxa; Salix hallaisanensis, Aristolochia controrta, Clematis trichotoma and Chrysosplenium barbatum. The plant resources were 186 taxa of ornamental plant, 241 taxa of edible plant, 274 taxa of medicinal plant and 202 taxa of others.