• Journal of Environmental Science International
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• v.14 no.3
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• pp.265-269
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• 2005

This study was carried out to provide the basic data for an identifying system for Iris species distributed in Korean market from complete analysing of genetic relationship between three native Iris species and one cultivar bred from the native Iris plant. RAPD analysis of genetic relationship among 4 Irises was possible. According to the RAPD analysis, they were divided into two groups. Among 4 Irises used in this study, Iris laevigata 'Veriegata', Iris laevigata and Iris setosa were classified into the same group since they had many similarities even though the habitat of Iris laevigata in Korean peninsular is restricted mainly in the south and Iris setosa is naturally inhabited in the northern part of Kangwondo. The value for the dissimilarity index of Iris laevigata and Iris laevigata 'Veriegata' was 6.757. The value for the dissimilarity index of Iris laevigata and Iris dichotoma was 95.000, so that they were genetically the farthest among them since the genetic relationship between two species are separated far if the value of the dissimilarity index is close to 100.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.10a
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• pp.24-24
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• 2019

Iris L. is a perennial genus comprising approximately 300 species worldwide, with the greatest number of endemic species occurring in Asia. Iris is one of the largest genera in the family Iridaceae and includes ca. 15 species native to Korea. Although chromosome number change, karyotype restructuring, and genome size variation play an important role in plant genome diversification, understanding the karyotype variation in Korean Iris species has been hampered by the wide range of base chromosome number (x = 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 22) reported to date. This study documents the chromosome numbers, karyotype structure and genome size variation in Iris ruthenica K. Gawl., an endangered species in Korea obtained using classic Feulgen staining and flow cytometry. The chromosome number of all investigated plants from the nine populations was 2n = 42. All individuals studied possessed metacentric and submetacentric chromosomes. The genome size of the I. ruthenica in eight wild populations ranged from 2.39 pg/1C to 2.45 pg/1C ($2.42{\pm}0.02pg/1C$: $mean{\pm}SD$). This study provides the first report of genome size variation in Iris ruthenica in Korea. This study lays foundation for cytogenetic further analyses employing by fluorescence in situ hybridization (FISH) to better understand the chromosomal evolution in this species and in the whole genus.

• Journal of Environmental Science International
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• v.19 no.11
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• pp.1337-1353
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• 2010

This study intended to investigate environmental factors including soil and vegetation in order to understand the environmental and ecological characteristics of 12 different habitats of Iris odaesanensis. These habitats, according to investigations, are mostly located at elevation of 280 m to 1,555 m with angles of inclination ranging from 2 degree to 30 degrees. A total of 273 vascular plants are identified in 23 quadrates of 12 habitats. Dominant species of woody plants in 12 habitats are represented as Quercus mongolica in the tree layer (T1) and the subtree (T2) layer, and Lespedeza maximowiczii, Lindera obtusiloba, Rhododendron schlippenbachii in the shrub (S) layer. The importance value of Iris odaesanensis is 9.65%, as regards the herbaceous layer, and 6 highly ranked species such as Carex siderosticta (3.92%), Meehania urticifolia (2.67%), Spodiopogon cotulifer (2.58%), Aconitum pseudolaeve (2.51%), Carex bostrychostigma (2.28%) and Disporum smilacinum (2.09%) are considered to be an affinity with Iris odaesanensis in their habitats. The degree of their average species diversity is 1.32, and that of dominance and evenness are 0.08 and 0.89, respectively. The type of soil is sandy loam and loam, and the average field capacity of soil is 28.31%. Their average organic matter is 16.71%, soil pH 5.29, and available phosphorus is 9.29%. Correlation coefficients analysis based on environmental factors, vegetation and soil analysis shows that the coverage of Iris odaesanensis is correlated with pH and dominance, and species richness is positive related with species diversity.

• Journal of Plant Biotechnology
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• v.31 no.1
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• pp.19-23
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• 2004

This study was conducted to observe the morphological characteristics and to analyse genetic relationship using RAPD markers in 9 species of Iris. The period of flowering was shown very broad among 9 species of Iris, from April 22 to June 9. The flower stalk was fluctuated from 9.0cm (I. rossii) to 104.0cm (I. ensata). I. ensata has the largest flower size and followed by I. laevigata, I. tectorum, I. sanguinea, I. pseudacorus, I. lactea, I. rossii, I. minutoaurea, I. odaesanensis. Among the 108 amplified bands by PCR with 8 random primers, 107 showed polymorphism and only one showed monomorphism among 9 species of Iris. The average dissimilarity coefficient among 9 species of Iris was 0.252. The range of dissimilarity coefficient was shown as from 0.095 to 0.609. Nine species were divided into three groups. The first group was I. tectorum, and the second group was I. lactea, I. laevigata, I. pseudacorus, I. odaesanensis. I. minutoaurea, I. rossii, I. sanguinea, I. ensata were belong to group three.

• Journal of Environmental Science International
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• v.15 no.12
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• pp.1103-1107
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• 2006

Iris odesanensis Y.N. Lee is one kind of species among 54 endangered plant species designated by the Ministry of Environment in Korea. It is very rare native plant throughout the country. Therefore, this study was conducted to investigate the native Iris odesanensis in Mt. Naeyon for their distribution and ecological characteristics. They were mostly distributed mid-slope of the mountain from 443m to 498m altitude. They were located lower part of valley and provided enough humid condition. They had proper light and humidity conditions as they faced south south west, south south east, and south. They were also located at the places deciduous woody plants were distributed for proper light conditions for spring blooming. Iris flowered to use light at the period before the trees thickly covered with foliage. However this unfavorable light condition caused low percentage as 7.04% of blooming individuals in this study. In herbaceous, Vioia chaerophylloides(Regel) W. Hocker, Carex siderosticta Hance, Erythronium japonicum Decne., Oxalis obtriangulata Max., Hepatica asiatica Nakai, Lilium tsingtauense Gilg et cetera were appeared well. We propose that proper pruning of upper branches of the deciduous trees is needed for suitable light condition of management of native sites.

• Korean Journal of Medicinal Crop Science
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• v.12 no.5
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• pp.401-405
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• 2004

Karyotypes were established in the eight Korean native species of the genus Iris. Chromosome numbers were 2n=50 in I. koreana and 2n=42 in I. uniflora var. carinata and their karyotype formulas were K = 2n = 50 = 14m + 28sm + 8st and K = 2n = 42 = 16m + 26sm, respectively. I. dichotoma and I. pseudoacorus were diploids of 2n=34. However, they showed different karyotype formulas: K = 2n = 34 = 26m + 6sm + 2st in I. dichotoma and K = 2n = 34 = 8m + 24sm + 2st in I. pseudoacorus. I. setosa, and I. pallasii var. chinensis carried the same chromosome numbers of 2n=40, but they showed different patterns of karyotype formula: K = 2n = 40 = 22m + 14sm + 4st in I. setosa and K = 2n = 40 = 26m + 12sm + 2st in I. pallasii var. chinensis. I. sanguinea was a diploid of 2n=28 and the karyotype formula was K = 2n = 28 = 14m + 14sm. I. ensata var. spontanea was a diploid of 2n=24 and the karyotype formula was K = 2n = 24 = 10m + 14sm. Each species showed characteristic chromosome composition with a pair of satellite chromosome except I. koreana with three pairs of satellite chromosomes. The chromosomes of I. dichotoma and I. uniflora were comparatively short, while the chromosomes of I. ensata were remarkably bigger than those of other species. These cytological data will give a useful information for the identification and breeding program of the Iris plants.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.6 no.1
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• pp.41-50
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• 2003

The feasibility as floricultural crops and water garden plant materials of Korean native water plants was investigated. Propagation type, growing and flowering time were observed for development as water garden, interior aquarium plant and water purification materials. Flowering time of the water plant was 7 species in May, 28 species in June, 49 species in July, 55 species in August, 47 species in September, and 17 species in October. Beautiful flowering water plants were Nymphaeaceae, Nymphoides peltata, Nymphoides indica, Monochoria korsakowii, Iris pseudacorus, Iris laevigata, and etc. Ornamental leafy water plants were Ceratopteris thalictroides, Ludwigia ovalis, Myriophyllum verticillatim, Limnophila sessiliflora, Blyxa aubertii, Blyxa echinosperma, Vallisneria asiatica, Hydrilla verticillata and Eleocharis acicularis etc. Isoetes japonica, Isoetes coreana and Isoetes sinensis were propagated by spore. Blyxa aubertii, Blyxa echinosperma, Myriophyllum verticillatim, Nuphar japonicum, Nelumbo nucifera, Ottelia alismoides, Sagittaria aginashi, Trapa japonica, and Trapa natans were propagated by seed. Persicaria amphibia, Ceratophyllum demersum (hornwort), Myriophyllum verticillatim, Myriophyllum spicatum, Oenanthe javanica, Potamogeton crispus, Hydrilla verticillata and Acorus calamus were propagated by division. And Vallisneria asiatica, Hydrilla verticillata and Phragmites japonica were propagated by runner. Ceratophyllum demersum (hornwort), Myriophyllum verticillatim, Myriophyllum spicatum, Limnophila sessilifera were propagated by adventitious bud. Ceratopteris thalictroides was propagated by leaf cutting. The 35 genera, 68 species of water plants were available for horticultural use. The 45 species such as Iris laevigata, Eleocharis acicularis, Menyanthes trifoliata, Nymphaea minima, Nuphar pumilum, Nymphoides coreana, Nymphoides peltata, Nymphoides indica, Nymphaea tetragona (water lily), and Typha latifolia could be use for water garden plant. The 21 species such as Limnophila sessilifera, Vallisneria asiatica, Ceratophyllum demersum and Hydrilla verticillata available for indoor aquarium. The 19 species such as Ottelia alismoides, Oenanthe javanica, Limnophila sessilifera and Blyxa echinosperma could be culture in container. The 27 species such as Trapa japonica, Trapa incisa, Phramites commuris (reed), Phragmites japonica, and Zizania latifolia were usable for water purification plant materials.

• Journal of Plant Biology
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• v.17 no.1
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• pp.33-35
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• 1974

This paper is proposed to describe a new species and two formae of flowering plants from central Korea. The taxa of new plants are as follow: Iris odaesanensis Y. Lee, sp. nov. Iris rossii Baker form. alba Y. Lee, form. nov. Ajuga multiflora Bunge form. rosea Y. Lee, form. nov.

• The Plant Pathology Journal
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• v.16 no.1
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• pp.36-42
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• 2000

The coat protein gene of iris severe mosaic potyvirus, which was isolated in Korea, ISMV-K, from iris plant was cloned and its nucleotide sequence was determined. The coat protein of the virus contained 252 amino acid residues, including five potential N-glyxosylation site motifs. The coat protein of ISMV-K has 99.1% and 98.4% sequence identities with those of the Netherlands isolate of ISMV (ISMV-Ne) form crocus for the nucleotide and amino acids, respectively. The coat protein of ISMV-K has 50.4% to 60.3% nucleotide sequence identities and 47.3% to 55.7% amino acid identities with those of other 21 potyviruses, indicating ISMV to be a distinct species of the genus. The coat protein of ISMV-K was closely related with bean yellow mosaic virus and clover yellow vein virus in the phylogenetic tree analysis among the potyviruses analyzed. ISMV was easily and reliably detected from virus-infected iris leaves by RT-PCR with a set of the virus-specific primers.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.10a
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• pp.32-32
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• 2019

The aim of this study was to provide basic guidelines for conservation and management of endangered plants in the national parks of Korea. Iris dichotoma Pall. (Iridaceae), which is a popular garden plant, is considered a second-class endangered species by Korean government and it is listed as a EN (Endangered) species in Red Data Book of Korea. We analyzed ecological conditions of I. dichotoma habitats based on vegetation properties and soil characteristics. This species which is known to inhabit in grassland adjacent to the ocean of lowlands slope and its population was located at an elevation of 8 m to 11 m. In the study sites, the mean of soil organic matter, total nitrogen and soil pH were 6.16%, 0.234% and 5.39 respectively. Additionally, the genetic variation and structure of three populations were assessed using ISSR (Inter Simple Sequence Repeat) markers. The genetic diversity of I. dichotoma (P = 59.46%, H = 0.206, S = 0.310) at the species level was relatively high. Analysis of molecular variance (AMOVA) showed 82.1% of the total genetic diversity was occurred in within populations and 17.9% variation among populations. Lastly, we developed predicted distribution model based on climate and topographic factors by applying SDMs (Species Distribution Models). Consequently, current status of I. dichotoma habitats is limited with natural factors such as the increase of the coverage rate of the herbs due to ecological succession. Therefore, it is essential to establish in situ and ex situ conservation strategies for protecting natural habitats and to require exploring potential and alternative habitats for reintroduction.