• 아시안잔디학회지
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• 제15권1호
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• pp.1-8
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• 2001

Antifungal activity of 20 chemicals registered to turfgrass diseases was evaluated. Among the chemicals, iprodione, benomyl, iprodione+thiram, pencycuron+tebuconazole, hexaconazole, and iprodione+thiophanate-methyl exhibited high antifungal activity to the dollar spot fungus. All isolates were greatly inhibited by the chemicals at the concentration over 32ppm($\mu\textrm{g}$/ml). However, sensitivity of the isolates to chemicals was varied at the lower concentration as 8 and 16 ppm as follows. The isolate originated from Ora golf course was resistant to iprodione and iprodione+thiophanate methyl, Gonjiam isolate to iprodion, Youngpyung isolate to iprodione+thiram, and Dogo isolate to iprodione+thiram, pencycuron+tebuconazole, and tebuconazole. It was found from this study that the varied chemical resistance among the isolates was positively related to the application time of the chemical in the golf course. Consequently, when a chemical was applied more often times than the others to the golf course, the fungal isolates originate from the field showed higher resistance to the former chemical. Effects of the chemicals on control of the dollar spot was evaluated in the field. All tested chemicals revealed over 70% disease control efficacy, however, mepronil+propiconazole was the best showing 83% control efficacy and followed by fenarimo, iprodione, terbuconazole, thiram, and thiophanate-methyl.

• 한국응용곤충학회지
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• 제23권2호
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• pp.89-95
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• 1984

딸기${\cdot}$겹무늬 Dendrophoma obscurans에 현저한 효과를 나타내는 thiophanate-methyl과 iprodione 제에 대하여 D. obscurans의 저항성균이 포장과 실험실에서 발생 및 저항성균에 의한 이들 약제의 방제효과 저하 여부등에 대하여 검토하였다. 1. 전국 주요 딸기 산지에서 분리한 본 균의 60개균주에 대하여 저항성검정을 실시한 결과 포장에서 thiophanate-methyl 저항성균의 발생율은 약 $43\%$로서 고도저항성균 SU1과 SU2외는 저도저항성균이었다. 이에 반하여 iprodione 저항성균의 발생율은 $10\%$이었고 모두 저도저항성균이었다. 2. in vitro에서 thiophanate-methyl 저항성균은 균계 단대배양 또는 자외선 처리에 의해서도 전현 분리되지 않았으나 iprodione 저항성균은 자외선 처리 없이도 iprodione $1{\mu}g/ml$ 또는 $10{\mu}g/ml$ 첨가 PDA 배지에서 용이하게 분리되었으며 모두 고도저항성균이었다. 3 딸기 잎에서 저항성균의 접종에 의한 thiophanate-methyl과 iprodione의 방제가는 저항성균 모두 저하되었으며 고도저항성균이 저도저항성균에 비하여 현저히 불량하였다. 4. thiophanate-methyl 저항성균은 benomyl에도 저항성을 보였으나 iprodione 저항성균은 thiophanate-methyl, benomyl, captan 및 zineb 등 공시약제에 모두 교차저항성을 보이지 않았으며, captan이 감성균은 물론 thiophanate-methyl 저항성균과 iprodione 저항성균을 효과적으로 방제하였다.

• 한국식물병리학회지
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• 제14권4호
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• pp.367-370
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• 1998

Monilina fructicola, the brown rot fungus of stone fruits, was isolated from overwintering mummies and peduncles on peach trees from February to March, 1998. The resistant population of these isolates to benzimidazole (benomyl, carbendazim and thiophanate-methyl) and dicarboximide (iprodione, vinclozolin and procymidone) was examined. Among 417 isolates, the incidence of isolates resistant to benomyl, carbendazim, and thiophanate-methyl were 45 (10.8%), 47 (11.3%), and 46 (11.0%), respectively. Forty two (10.0%) isolates showed cross-resistance to benzimidazole fungicides. On the other hand, the resistant isolates against iprodione, vinclozolin and procymidone were 186 (44.6%), 1 (0.2%) and 150 (36.0%), respectively. Among the isolates, 116 (27.8%) showed cross-resistance to iprodione and procymidone. Moreover, 27 (6.5%) of 417 isolates showed double-resistance to both benzimidazole (benomyl) and dicarboximide (iprodione).

• 한국식물병리학회지
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• 제3권4호
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• pp.270-276
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• 1987

울산지방의 농가과수원 사과나무 나병엽에서 분리한 Alternaria mail Roberts의 iprodione 저항성균주의 EC50은 $550\~1,310{\mu}g/ml$였다. 포자발아율은 iprodione $500{\mu}g/ml$를 가한 PSA에서 $70\%$였다. 저항성균주를 접종한 사과에 동약제 $500{\mu}g/ml$ 용액을 처리하였을 때 7일후 병반직경이 1.6-14.6mm였으나 감수성균주는 0mm였다. Benomyl, Chlorothalonil, garbenda, thiophanatemethyl 및 triademefon에 대해서는 이들 저항성균주가 감수성균주와 비슷한 경향의 반응을 보였으나 저항성균주 $R_3$는 밤수성균주에 효과적인 captan과 folpet이 대하여 교착저항성을 나타내었다.

• The Plant Pathology Journal
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• 제18권4호
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• pp.216-220
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• 2002

Seed decay of soybean caused by Diaporthe phaseolorum, Colletotrichum truncatum and Cercospora kikuchii is a serious disease when soybean is harvested under warm and wet weather conditions. Benomyl has been used for controlling the disease, however, benomyl application may be limited due to common occurrence of resistance. The efficacy of 21 fungicides against the pathogens was evaluated in vitro. Among the fungicides tested, benomyl, carbendazim, fluazinam, iprodione+propineb, thiophanate-methyl, and triflumizole were found effective and were evaluated for their ability to control the seed pathogens. Fluazinam completely inhibited mycelial growth at a concentration of 100 $\mu\textrm{g}$/$\textrm{m}{\ell}$ for D. phaseolorum; and at a concentration of 500 $\mu\textrm{g}$/$\textrm{m}{\ell}$ for C. truncatum and C. kikuchii. $EC_90$ values of fluazinam were similar to that of benomyl. Because fluazinam, iprodione+propineb, and triflumizole were found effective against the seed pathogens, these were subjected for field-testing. Suppression of pod and seed infection by fluazinam and iprodione+propineb was as high as that of benomyl without any reduction in agronomic characters of soybean. This study shows that fluazinam and iprodione+propineb may be used in combination with benomyl to control seed pathogens, manage resistance, and ensure production of high quality soybean seeds.

• 아시안잔디학회지
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• 제24권2호
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• pp.170-175
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• 2010

우리나라 16개 골프장으로부터 동전마름병균 24균주를 분리하였다. Sclereotiona homoeocarpa 24 균주의 약제 저항성 검정은 지오판수화제(benzimidazole계)와 테부코나졸유제(demethylation inhibitor: DMI), 이프로디온수화제(dicaboximide계)가 첨가된 영양배지 위에서 균사 생장에의해 결정되었다. 시험결과 24 균주 중 이프로디온수화제에 약제 저항성 발생률은 83.3%, 지오판수화제 62.5%, 테부코나졸유제 0%를 나타냈었다. 또한 이프로디온수화제와 지오판수화제 두 약제에 대한 약제 저항성 발생률은 58.3%였다. 지오판수화제와 이프로디온수화제의 살균제 저항성 발생률은 잔디 초종과 병원균 분리장소와 상관이 없었다. 동전마름병 방제를 위한 포장시험에서 프로사이미돈(prcymidone), 보스칼리드(boscalid), 플루퀸코나졸+테부코나졸(fluquinconazole+pyrimethanil)이 크리핑벤트그래스의 동전마름병을 효과적으로 방제하였다.

• The Plant Pathology Journal
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• 제33권1호
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• pp.95-100
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• 2017

Fungicide-resistant Alternaria alternata impede the practical control of the Alternaria diseases in crop fields. This study aimed to investigate cytological fungicide resistance mechanisms of A. alternata against dicarboximide fungicide iprodione. A. alternata isolated from cactus brown spot was cultured on potato-dextrose agar (PDA) with or without iprodione, and the fungal cultures with different growth characteristics from no, initial and full growth were observed by light and electron microscopy. Mycelia began to grow from one day after incubation (DAI) and continued to be in full growth (control-growth, Con-G) on PDA without fungicide, while on PDA with iprodione, no fungal growth (iprodione-no growth, Ipr-N) occurred for the first 3 DAI, but once the initial growth (iprodione-initial growth, Ipr-I) began at 4-5 DAI, the colonies grew and expanded continuously to be in full growth (iprodione-growth, Ipr-G), suggesting Ipr-I may be a turning moment of the morphogenetic changes resisting fungicidal toxicity. Con-G formed multicellular conidia with cell walls and septa and intact dense cytoplasm. In Ipr-N, fungal sporulation was inhibited by forming mostly undeveloped unicellular conidia with degraded and necrotic cytoplasm. However, in Ipr-I, conspicuous cellular changes occurred during sporulation by forming multicellular conidia with double layered (thickened) cell walls and accumulation of proliferated lipid bodies in the conidial cytoplasm, which may inhibit the penetration of the fungicide into conidial cells, reducing fungicide-associated toxicity, and may be utilized as energy and nutritional sources, respectively, for the further fungal growth to form mature colonies as in Ipr-G that formed multicellular conidia with cell walls and intact cytoplasm with lipid bodies as in Con-G.

• The Plant Pathology Journal
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• 제24권4호
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• pp.447-452
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• 2008

Four hundred and sixty six isolates of B. cinerea were obtained from infected leaves, stems and fruits of paprika grown in greenhouses or plastic film houses in Gangwon province, Korea, between August and November in 2006 and 2007. These isolates were classified into five representative phenotypes of resistant (R) and sensitive (S) reactions as SSR, SRR, RSS, RRS and RSR according to the responses of isolates against benzimidazole, dicarboximide and N-phenyl-carbamate fungicide in order. The percentage of five phenotypes were 51.3, 2.4, 35.6, 8.1 and 2.6%, respectively. The SSR phenotype (51.3%) was the most common. Among the nineteen fungicide products evaluated to compare their efficacy against gray mold pathogen on the paprika fruit inoculated with fungal mycelia, the mixture of diethofencarb and carbendazim was the most effective followed by iprodione, boscalid, the mixture of iprodione and thiophanate-methyl, fludioxonil, polyoxin-B, fluazinam, the mixture of tebuconazole and tolyfluanid and procymidone; while in the assay methods inoculated with fungal spores, the mixture of tebuconazole and tolyfluanid was the most effective in controlling gray mold followed by boscalid, fludioxonil, the mixture of diethofencarb and carbendazim and the mixture of pyrimethanil and chlorothalonil.

• 한국식물병리학회지
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• 제14권6호
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• pp.682-688
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• 1998

Fungicide resistance of 48 isolates of Botrytis cinerea collected from citrus in Cheju was investigated and genetic diversity was analyzed with random amplified polymorphic DNA(RAPD). High levels of resistance to benzimidazole fungicides benomyl and thiophanate-methyl and N-phenylcarbamate fungicide diethofencarb were observed. Negative cross resistance was clear between benzimidazole and N-phenylcarbamate fungicides, and multiple resistance to the fungicides was also observed. There was cross resistance among the dicarboximide fungicides procymidione, vinclozolin and iprodione as it was observed between the benzimidazole fungicides benomyl and thiophanate-methyl. The lowest levels of resistance were to the dicarboximide fungicides, but no sensitive isolate to polyoxin B was observed. The isolates showed genetically diverse RAPD profiles according to the geographic origin collected, but there was no significant correaltion between RAPD profiles of genomic DNA and the levels of fungicide resistance of the isolates. The isolates showed genetically diverse RAPD profiles, indicating that genetic differentiation had already occurred in the populations of B. cinerea distributed in Cheju.

• 식물병연구
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• 제19권1호
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• pp.7-11
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• 2013

국내 나리 주산단지에서 분리한 Botrytis elliptica 48개 균주와 Botrytis cinerea 23개 균주를 대상으로 농가포장에서 사용되고 있는 살균제에 대한 약제저항성 검정을 실시하였다. 살균제 benomyl과 mancozeb에 대한 $EC_{50}$ 값은 $500-1,000{\mu}g/ml$로 시험에 사용된 모든 약제 중에서 가장 높게 나타나 이 약제들은 나리재배 포장에서 잎마름병에 대한 방제 효과가 거의 없을 것으로 판단되었다. 또한 나리 재배농가에서 잎마름병 방제약제로 가장 많이 사용되고 있는 dicarboximide계 procymidone 살균제와 iprodione 살균제의 경우, $EC_{50}$ 값 $5-50{\mu}g/ml$은 각각 93.7%와 100%이었고, diethofencarb/carbendazim 합제와 fludioxonil 살균제의 $EC_{50}$ 값 $0-0.1{\mu}g/ml$ 범위는 각각 98.0%와 93.8%이었다. 한편, 비가림 포장 재배에서의 잎마름병 방제 효과는 발병 직후 iprodione 약제, diethofencarb/carbendazim 합제, fludioxonil 약제를 교호적으로 4회 살포하였을 때 가장 우수하였다.