• Title/Summary/Keyword: Ipomoea

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Enzymatic Release of Ferulic Acid from Ipomoea batatas L. (Sweet Potato) Stem

  • Min, Ji-Yun;Kang, Seung-Mi;Park, Dong-Jin;Kim, Yong-Duck;Jung, Ha-Na;Yang, Jae-Kyung;Seo, Won-Teak;Kim, Seon-Won;Karigar, Chandrakant S.;Choi, Myung-Suk
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.11 no.4
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    • pp.372-376
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    • 2006
  • Ferulic acid is a phenolic compound that serves as a major biosynthetic precursor of vanillin in higher plants. We investigated the ability of the 3 commercial enzymes - Ultraflo L, Viscozyme L, and ${\alpha}-Amylase$ - to induce the release ferulic acid from the Ipomoea batatas L. (sweet potato) stem. The rate of release for ferulic acid was optimal when Ultraflo L (1.0%) was used compared with the other enzymes, whereas Viscozyme L was most effective for the release of vanillic acid and vanillin. Thus, these enzymes may be useful for the large-scale production of ferulic acid and other phenolic compounds from sweet potato stem.

Current Status and Prospect of Quality Evaluation in Sweetpotato (고구마 품질평가 현황과 전망)

  • 정병춘;안영섭;정미남;이준설;오양호
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.47
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    • pp.124-134
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    • 2002
  • The sweetpotato, Ipomoea batatas L. (Lam.), is one of the important summer upland crops in Korea and has been used as human food, industrial yaw material and vegetable. Sweetpotato has been consumed for human foods such as boiled, roasted, fried or salad etc. It should be developed for higher quality as a snack or health food, primarily through improving the eating and marketing qualities as well as nutritional value. Its quality after cooking or processing is a complex one combining the aroma, taste, texture and fiber content. The other important qualities for consumers are root shape, size, skin color, flesh color, insect and disease resistance, nutritional components and safety from phytoalexins(toxic stress metabolites) etc. Korean people generally prefer to red skin color, round or elliptic shape and dry texture, yellow flesh color of sweetpotato which is high in starch content including vitamins and nutrients. The almost factors of quality components of sweetpotato are genetically controlled by breeder, but postharvest handlings and marketing management for making high quality goods should be done thoroughly according to the quality evaluation criteria of sweetpotato from the moment of harvest until shipping them to the market by farmers and the other users. This paper describes current status and prospects of the quality evaluations and researches in sweetpotato roots in Korea.

Changes in Antioxidant Activity and Total Phenolic Content of Water Spinach (Ipomoea aquatic Forsk.) under In Vitro Biomimicking System

  • Lee, A-Young;Kim, Young-Suk;Shim, Soon-Mi
    • Food Engineering Progress
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    • v.14 no.4
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    • pp.342-345
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    • 2010
  • The purpose of current study was to examine bioaccessibility of antioxidant activity and total phenolic content in each part of water spinach (Ipomoea aquatic Forsk.). In vitro biomimicking system simulated human digestive fluid was employed in order to measure bioavailable anti-oxidative effect and phenolic content. Antioxidant activity and total phenolic content was measured by using the DPPH method and the Folin-Ciocalteu assay, respectively. Stem of water spinach had a higher DPPH free radical scavenging effect (5.43 mg/mL for $IC_{50}$) than leaf (5.95 mg/mL for $IC_{50}$), while leaf had a greater level of total phenolic content (287.45 ${\mu}g$ GAE/mL) than stem (216.45 ${\mu}g$ GAE/mL). Bioaccessible antioxidant capacity and digestive stability of total phenolic content showed a similar pattern to what found in raw materials. Our result also indicated that total phenolic content was not found to be a major marker for prediction of antioxidant activity. It is plausible that other constituents such as vitamin E and C in water spinach could be contributors for antioxidant activities.

Synergy effect of legal highs with antibiotics (Legal High Plants와 항생제의 항균활성 비교)

  • Jung, Son-Hyo;You, Seon-Hee;Park, Cho-Hi
    • Journal of the Korean Applied Science and Technology
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    • v.37 no.6
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    • pp.1635-1645
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    • 2020
  • In this study would like to find extending or increasing the efficacy of the antibiotic substance for the strains with resistance to antibiotics or persister cells by inhibition of the resistance. This study was used different species of 'legal high' plants leaves from Leonotis leonurus, Mitragyna speciosa, and seeds from Ipomoea murucoides with antibiotics which are Amoxicillin, Chloramphenicol, Ciprofloxacin, Kanamycin, Oxacillin, and Vancomycin. Legal highs were extracted with methanol. Minimum inhibitory concentration(MIC) testing for a range of antibiotics with extracts of plant was fulfilled by broth dilution methods. In this essay, it was determined in a microdilution assay utilizing suspended in ISB up to a final concentration of 512㎍/ml in 96 wells microtitre plates, threefold and serial dilutions. After that, the microplates were kept in incubator between 35℃ and 37℃ for overnight. Leonotis leonurus, Mitragyna speciosa, and Ipomoea murucoides of Legal highs (512㎍/ml) investigated small activity to inhibit against pathogens which are susceptible Staphylococcus aureus, resistant Staphylococcus aureus, susceptible Enterococcus faecalis, resistant Escherichia coli.

Expression Analysis of Sweetpotato Sporamin Genes in Response to Infection with the Root-Knot Nematode Meloidogyne incognita

  • Jung-Wook Yang;Yun-Hee Kim
    • Journal of Plant Biotechnology
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    • v.50
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    • pp.163-168
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    • 2023
  • Sweetpotato (Ipomoea batatas [L.]) is a globally important root crop cultivated for food and industrial processes. The crop is susceptible to the root-knot nematode (RKN) Meloidogyne incognita, a major plant-parasitic RKN that reduces the yield and quality of sweetpotato. Previous transcriptomic and proteomic analyses identified several genes that displayed differential expression patterns in susceptible and resistant cultivars in response to M. incognita infection. Among these, several sporamin genes were identified for RKN resilience. Sporamin is a storage protein primarily found in sweetpotato and morning glory (Ipomoea nil). In this study, transcriptional analysis was employed to investigate the role of sporamin genes in the defense response of sweetpotato against RKN infection in three susceptible and three resistant cultivars. Twenty-three sporamin genes were identified in sweetpotato and classified as group A or group B sporamin genes based on comparisons with characterized sweetpotato and Japanese morning glory sporamins. Two group A sporamin genes showed significantly elevated levels of expression in resistant but not in susceptible cultivars. These results suggest that the elevated expression of specific sporamin genes may play a crucial role in protecting sweetpotato roots from RKN infection.

Antioxidant Activities of Ipomoea batatas L. Lam. (Purple Sweet Potato) Extracts Cultured in Korea (국내산 자색고구마(Ipomoea batatas L. Lam.) 추출물의 항산화활성 평가)

  • Kong, Bong Ju;Han, Sung Soo;Ha, Ji Hoon;Park, Soo Nam
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.40 no.4
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    • pp.423-430
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    • 2014
  • In this study, the antioxidative effects for the extracts of purple sweet potato (Ipomoea batatas L. Lam.) were investigated. The purple sweet potato was extracted with 70% ethanol and the ethyl acetate fraction was obtained from the extracts. The yields of extract and ethyl acetate fraction were 39.2% and 3.49% per dried powder, respectively. To confirm the antioxidative effects of the extracts, free radical scavenging activities (1,1-diphenyl-2-picrylhydrazyl), total antioxidant capacity by luminol-dependent chemiluminescence assay and the protective effects against reactive oxygen species (ROS) in erythrocytes were measured. Free radical scavenging activities ($FSC_{50}$) of the 70% ethanol extract and ethyl acetate fraction were $90.16{\mu}g/mL$ and $7.69{\mu}g/mL$, respectively. The free radical scavenging activity of ethyl acetate fraction was higher than that of (+)-${\alpha}$-tocopherol ($8.98{\mu}g/mL$). Total antioxidant capacities ($OSC_{50}$) of the 70% ethanol extract and ethyl acetate fraction were $5.75{\mu}g/mL$ and $1.92{\mu}g/mL$, respectively. The capacity of ethyl acetate fraction was similar to L-ascorbic acid, known as a prominent water soluble antioxidant ($1.50{\mu}g/mL$). The cellular protective effects of the ethyl acetate fractions on the $^1O_2$-induced cellular damage of human erythrocytes were increased in a concentration dependent manner ($5{\sim}50{\mu}g/mL$). The ${\tau}_{50}$ value in $5{\mu}g/mL$ was 45.6 min which was higher than that of (+)-${\alpha}$-tocopherol in all concentrations. These results indicate that the ethyl acetate fraction of purple sweet potato (I. batatas) has the excellent antioxidative capacity and could be applicable to anti-aging cosmeceutical ingredients for skin aging inhibition.

Effects of Varieties, Growth Duration, and Topping for Occurrence of Skin Ridge on the Tuberous Root in Sweet Potato (Ipomoea batatas Lam.) (고구마 품종, 생육기간 및 경엽제거가 피맥발생에 미치는 영향)

  • Choi, Kyu-Hwan;Yu, Young-Jin;Kim, Hyo-Jin;Kang, Chan-Ho;Jeong, Jong-Sung;Song, Young-Ju;Kim, Chung-Kon;Lee, Seung-Yeop
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.59 no.2
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    • pp.194-200
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    • 2014
  • Skin ridge (SR) is a irregular line shape that stand out in epidermal layer on sweet potato (Ipomoea batatas Lam.). SR reduced marketable value of storage root in sweet potato. It would be occurred by accumulation of starch in epidermal layer. SR rate was more highly increased in early planting. SR rate was under 3% in 7 varieties 'Geonpoongmi', 'Yeonhwangmi', 'Geonmi' etc and the rate of two varieties 'Healthymi', 'Borami' were up to 30%. SR rate was positively correlated with vine weight. SR rate was the highest in 120 days after planting. As leaf area index was reduced to 3, 4, 5 and 6 (non-topping) by topping, it was hard to fine SR occurrence in topping treated sweet potato. Topping treatment, however, caused decrease in yield overall. Therefore, topping treatment for decreasing of SR was not recommended in agricultural practice of sweet potato.

Improvement of Peroxidase Productivity by Optimization of Medium Composition and Cell Inoculum Size in Suspension Cultures of Sweet Potato (Ipomoea batatas) (고구마(Ipomoea batatase)현탁배양에서 배지조성 및 세포접종량의 적정화에 의한 Pemxidase생산성 향상)

  • 곽상수;김수경;정경희;유순희;박일현;유장렬
    • Korean Journal of Plant Tissue Culture
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    • v.21 no.2
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    • pp.91-97
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    • 1994
  • To improve the productivity of peroxidase (POD) of cell line SP-47 derived from cell suspension cultures of sweet potato (Ipomoea batatas (L) Lam.cv White Star), we optimized culture conditions including the composition and concentration of plant growth regulators and carbon source, and the cell inoculum size. When one g (fr wt) of cells was inoculated into 50 mL TL medium supplemented with l mg/L 2,4-D and 30g/L sucrose in 300 mL Erlenmeyer flask at 25$^{\circ}C$ in the dark (100rpm), the POD activity per g cell dry wt was maximized to be about 6,800 units after 25 days of subculture, which was about 30 times higher than that of intact roots of horseradish plants grown in the greenhouse, but the cell growth was maximum after 15 days of subculture. The protein content per g cell dry wt maintained almost plateau and after 25 days of subculture decreased as culture Proceeded further whereas the POD specific activity (unit/mg protein) was about two times higher after subculture and continuously increased from 12 days to the end of cultures (40 days). The POD isozyme patterns showed almost the same regardless of cell growth stage, but some acidic isozymes were slightly increased after 25 days of subculture. These results indicate that POD activity in suspension cultures of sweet potato is closely associated with cell growth and stresses derived from cell culture renditions and medium depletion. Due to its high POD activity the SPL47cell line seems to be suitable for the mass production of POD.

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