• Reproductive and Developmental Biology
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• v.32 no.2
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• pp.71-79
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• 2008

Surface-enhanced laser desorption and ionization time-of-flight mass spectrometry (SELDI-TOF MS) is one of the recently developed proteomic technologies which is based on capturing proteins and peptides by chemically modified surfaces and highly sensitive for the analysis of complex biological samples. In the present study, to gain insights into oocyte maturation and early embryo development, SELDI-TOF-MS was used to find the protein candidates that are specifically or prominently expressed in porcine oocytes at the in vitro matured metaphase II (MIIl) and germinal vesicle (GV) stages. By selected CM10 chip, 16 candidates were found to be up-regulated in GV stage oocytes compared with in MII stage oocytes, their molecular weights were 8,180 (2 candidates), 10,226 (5 candidates), 15,767 (5 candidates) and 16,770 (4 candidates) Da respectively. And the expression of 29 candidates were higher in MII than in GV stage oocytes, their molecular weight were 10,832 (3 candidates), 17,743 (8 candidates), 20,122 (3 candidates), 22,131 (3 candidates), 24,857 (7 candidates) and 33,507 (5 candidates) Da, respectively. The expression of selected 13 candidates (0.2 and 1.0 % error tolerances) were analyzed using real time RT-PCR. The proteins that differentially regulated during oocyte in vitro maturation in the pigs may be potential biomarkers of oocyte maturation and quality.

• Journal of the Korean Applied Science and Technology
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• v.17 no.1
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• pp.63-66
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• 2000

Organic Electroluminescent devices(OELD) consisted of multilayer structures have been studied for the application the application to flat-panel display. Metal-chelate complexes, zinc bis(2-(2-hydroxyphenyl)benzoxazolate) ($Zn(BOX)_{2}$) and zinc bis(2-(2-hydroxyphenyl)benzothiazolate) ($Zn(BOX)_{2}$), have been intensively investigated as an white-light emitting layer and recognized to have good electroluminescent(EL) properties. In this study, ($Zn(BOX)_{2}$) and ($Zn(BTZ)_{2}$) were synthesized and characterized by FT-IR, $^{1}H-NMR$, UV-VIS and PL. Their EL properties were also studied and their ionization potential(IP) and electron affinity(EA) were also measured by cyclic voltammetry(CV).

• KIEE International Transactions on Electrophysics and Applications
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• v.4C no.1
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• pp.5-9
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• 2004

In this paper, pulsed discharge is used to control the luminous color in gas discharge tubes. The luminous color of the positive column in gas discharge tubes filled with Hg-Ar-Ne (1: 9, 60［Torr］) and having no phosphor material, varies from red to blue emitted by the Ne and Hg from the pulsed discharge. With changing of pulse-width and frequency, the electron temperature in the transient period affects changes to the residual ion and metastable atom densities. The first metastable atoms containing energy levels of about 16.6 ［eV］have a very high probability that a collision will result in the ionization potential of Ar being 15.8 [eV]. The change of locus in the CIE chromaticity diagram with increasing pulse-width and frequency approves the variation of luminous color.

• Journal of the Institute of Electronics Engineers of Korea SC
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• v.48 no.3
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• pp.53-59
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• 2011

Charge transfer through DNA oligonucleotides has been investigated for potential applications of DNA into molecular electrooptic switching devices. Electrons were injected using gold electrode probes where DNA oligomers were adsorbed that are separated in medium. The results show that increased adsorption of DNA reduces the ionization current due to the combined effect of charge transfer through DNA and surface-limited charge transport. The probe-based charge injection was extended to examine the capability of extinguishing fluorescence of Cy3 dye molecules attached to DNA. It is expected that the results may be employed to implementing a novel electrooptic switching device based on DNA molecules.

• Journal of the Korean earth science society
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• v.36 no.5
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• pp.419-426
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• 2015

We investigated the high dispersion spectra that had been secured at the center of the planetary nebula NGC 7027 with the Bohyunsan Optical Echelle Spectrograph (BOES) on October, 20, 2009. We analyzed the forbidden lines of [OI], [SII], [OII], [NII], [ClIII], [ArIII], [OIII], [ArIV], [NeIII], [ArV], and [CaV] in the $3770-9225{\AA}$ wavelength region. The expansion velocities were derived from double Gaussian line profiles of the emission lines, after eliminating the subsidiary line broadening effects. The radial variations of the expansion velocities were obtained by projecting the derived expansion velocities: $19.56-31.93kms^{-1}$ onto the equatorial shell elements of the inner and the outer boundaries of the main shell of 2.5(2.1)" and 3.8(3.6)", according to the ionization potential of each ion. Analysis of equatorial shell spectra indicated that the equatorial shell generally expands in an accelerated velocity mode, but the expansion pattern deviates from a linear velocity growth with radial distance. NGC 7027, of which age is about 1000 years or less, might be still at its early stage. During the first few hundred years, plausibly in its early stage, the main shell of PN expands very slowly and, later, it gradually gain its normal expansion speed.

• Journal of Radiation Protection and Research
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• v.11 no.1
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• pp.15-21
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• 1986

The chemical effects resulting from the capture of the thermal neutrons by manganese in different crystalline permanganates, that is, potassium permanganate, sodium permanganate, silver permanganate, barium permanganate and ammonium permanganate, have been investigated. The distribution of radioactive manganese formed has been determined by using different absorbents and ion-exchangers, that is, manganese dioxide, alumina, Zeolite A-3, Kaolinite and Dowex-50. The distribution of radioactive manganese in various adsorbents and ion-exchangers has almost similar result for each permanganate. The affinity for radioactive manganous ion is greatest for Dewex-50. A significant increase of retention is shown through the thermal annealing and the retention depends on the first ionization potential of metal ion in permanganates.

• KIEE International Transactions on Electrophysics and Applications
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• v.2C no.5
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• pp.246-252
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• 2002

Two-dimensional steady state simulations of planar type radio frequency inductively coupled plasma (RFICP) have been performed. The characteristics of RFICP were investigated in terms of power transfer efficiency, equivalent circuit analysis, spatial distribution of plasma density and electron temperature. Plasma density and electron temperature were determined from the equations of ambipolar diffusion and energy conservation. Joule heating, ionization, excitation and elastic collision loss were included as the source terms of the electron energy equation. The electromagnetic field was calculated from the vector potential formulation of ampere's law. The peak electron temperature decreases from about 4eV to 2eV as pressure increases from 5 mTorr to 100 mTorr. The peak density increases with increasing pressure. Electron temperatures at the center of the chamber are almost independent of input power and electron densities linearly increase with power level. The results agree well with theoretical analysis and experimental results. A single turn, edge feeding antenna configuration shows better density uniformity than a four-turn antenna system at relatively low pressure conditions. The thickness of the dielectric window should be minimized to reduce power loss. The equivalent resistance of the system increases with both power and pressure, which reflects the improvement of power transfer efficiency.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.18 no.12
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• pp.1139-1142
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• 2005

Recently, organic light emitting diodes(OLEDs) is widely used as one of the information display techniques. We synthesized 2-(2-hydroxyphenyl)benzoxazole($Zn(HPB)_2$). We studied the luminescent properties of OLEDs using $Zn(HPB)_2$. The ionization potential(IP) and the electron affinity(EA) of $Zn(HPB)_2$ investigated using cyclic-voltammetry(C-V). The IP and EA were 6.5 eV and 3.0 eV, respectively. The PL and EL spectra of $Zn(HPB)_2$ were observed at the wavelength of 450 nm. We used $Zn(HPB)_2$ as an emitting layer and hole blocking layer. At the experiment about hole blocking effect, we inserted $Zn(HPB)_2$ between emiting material layer(EML) and cathode, and between hole transport layer(HTL) and emitting material layer(EML). We measured current density-voltage and luminance-voltage characteristics at room temperature.

• The Bulletin of The Korean Astronomical Society
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• v.35 no.2
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• pp.40.2-40.2
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• 2010

Polycyclic aromatic hydrocarbon (PAH) features have emerged as one of the most important infrared (IR) features since these PAH features dominate mid-IR spectra of galaxies and are ubiquitous within galactic and extragalactic objects. These PAH features have the potentials to become reliable star formation rate (SFR) proxies and diagnostics of physical conditions of interstellar medium, such as ionization states of dust grains and grain sizes. While constructing an unbiased library of 44 sample galaxies selected from 5MUSES sample, AKARI mJY Unbiased Survey of Extragalactic Sources in 5MUSES (AMUSES) intends to measure and to calibrate the PAH 3.3 mm emission feature which has not been studied extensively due to its weak strength and dearth of capable instruments. Out of 20 target galaxies, we detected the 3.3mm feature from eight galaxies and measured their line strengths, line widths and line ratios with other PAH emission features. Sample galaxies whose spectral energy distributions (SEDs) are classified as starburst-type have clearly stronger 3.3mm emission features than ones with AGN-type SEDs. We also found that there is a correlation between the PAH 3.3mm luminosity and total IR luminosity within our sample galaxies, albeit a large scatter. We further discuss implications of our results.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.5
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• pp.1911-1915
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• 2012

Aim: New technologies for the early detection of pancreatic cancer (PC) are urgently needed. The aim of the present study was to screen for the potential protein biomarkers in serum using proteomic fingerprint technology. Methods: Magnetic beads combined with surface-enhanced laser desorption/ionization (SELDI) TOF MS were used to profile and compare the protein spectra of serum samples from 85 patients with pancreatic cancer, 50 patients with acute-on-chronic pancreatitis and 98 healthy blood donors. Proteomic patterns associated with pancreatic cancer were identified with Biomarker Patterns Software. Results: A total of 37 differential m/z peaks were identified that were related to PC (P < 0.01). A tree model of biomarkers was constructed with the software based on the three biomarkers (7762 Da, 8560 Da, 11654 Da), this showing excellent separation between pancreatic cancer and non-cancer., with a sensitivity of 93.3% and a specificity of 95.6%. Blind test data showed a sensitivity of 88% and a specificity of 91.4%. Conclusions: The results suggested that serum biomarkers for pancreatic cancer can be detected using SELDI-TOF-MS combined with magnetic beads. Application of combined biomarkers may provide a powerful and reliable diagnostic method for pancreatic cancer with a high sensitivity and specificity.