• Title/Summary/Keyword: Ion uptake

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Studies on the effect of silicate on nutrients up take using radioisotopes in rice plant. (I) (RI를 이용한 규소시용이 수도의 영양필요흡수에 미치는 영향(I))

  • Choon-Johong Ro
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.12
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    • pp.15-20
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    • 1972
  • There are still many problems on the physiological role of silicon in rice plants, although, it has been reported that silicate slag is the most effective fertilizer for the improvement of Akiochi soils. This experiment is carried out to investigate the effect of silicate of the mineral nutrition uptake by rice plants, using Ca45, P32, C136, and Mn54. The obtained results are summerized as follows; 1. Contents of mineral nutrients except silicon were higher in the rice plant grown in minus Si-culture solution a than in plus Si-plot. 2. Transpiration amounts appeared to be greater in the low content plant of silicon. 3. The rate of translocation to shoot of each ion absorbed by roots depended on the rate of transpiration. 4. It seemed that only CI uptake was found small in minus Si-root. 5. Silicate fetilizer did not affect the solubility of mineral ions in soils.

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The Effects of Anion Replacement on Proteins, Sugars and Nitrate Concentration in Italian Ryegrass (Lolium multiflorum L.) (음이온 대체공급이 이탈리안 라이그라스의 단백질, 당 및 질산염 농도에 미치는 영향)

  • 이복례;정우진;김기원;김태환
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.20 no.4
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    • pp.265-274
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    • 2000
  • To investigate the effects of anion replacement on NO3- concentration in Italian ryegrass. Plants weregrown hydroponically to the full vegetative stage. NO3 supply(control) was replaced with SO1- (Tl), C1-(T2) and water (T3) to during 14 days. The determination of inorganic nutrient uptake and quantification ofprincipal metabolites (nitrate. protein and sugar) followed. Relatively high uptake of ~ a a'n d Ca" for controlplants, K+ and POJ- for T2 plants, and C 1 for TI plants was observed, respectively. Proteins in shoot andstubble were relatively higher in control and TI plants, which coupled N source. In root proteins largelydecreased (especially in T3 plants) during experimental period. Sugars in shoot of all four treatments tendedto decrease during the first 7 days and recovered afterward. Sugars in stubble also markedly decreased duringthe first 7 days, while those in root was much less varied during experimental period. After 14 days oftreatment, nitrate concentration in shoot of control plants was 13mgIg FW. Comparing to control: nitrate inshoot reduced by 27%, 46% and 50% in TI, T2 and T3 plants, respectively. Dry weight was slightlyincreased or not significantly changed in control, T1 and T2 plants, while a significant decrease(31.3% ofcontrol) occurred in T3 plants.(Key words : Italian ryegrass, Anion replacement, Ion uptake, Protein, Sugar, Nitrate)ptake, Protein, Sugar, Nitrate)

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Adsorption Characteristics of Ni2+, Zn2+ and Cr3+ by Zeolite Synthesized from Jeju Scoria (제주 스코리아로부터 합성한 제올라이트에 의한 Ni2+, Zn2+ 및 Cr3+의 흡착 특성)

  • Kim, Jung-Tae;Lee, Chang-Han;Kam, Sang-Kyu
    • Journal of Environmental Science International
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    • v.29 no.7
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    • pp.739-748
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    • 2020
  • The characteristics of heavy metal ion (Ni2+, Zn2+, and Cr3+) adsorption by zeolite synthesized from Jeju scoria using the fusion and hydrothermal method, were studied. The synthetic zeolite was identified as a Na-A zeolite by X-ray diffraction analysis and scanning electron microscopy images. The equilibrium of heavy metal ion adsorption by synthetic zeolite was reached within 60 min for Ni2+ and Zn2+, and 90 min for Cr3+. The uptake of heavy metal ions increased with increasing pH in the range of pH 3-6 and the uptake decreased in the order of Cr3+ > Zn2+ > Ni2+. For initial heavy metal concentrations of 20-250 mg/L at nonadjusted pH, the adsoption of heavy metal ions was well described by the pseudo second-order kinetic model and was well fitted by the Langmuir isotherm model. The maximum uptake of heavy metal ions obtained from the Langmuir model, decreased in the order of Zn2+ > Ni2+ > Cr3+, differing from the effect of pH on the uptake, which was mainly based on the different pH of the solutions.

Purification of Methioninase from Pseudomonas putida and Its Effect on the Uptake of ^11C-Methionine in Vivo. (Pseudomonas putida 유래 Methioninase의 정제 및 생체내 ^11C-Methionine 섭취에 미치는 영향)

  • 변상성;박귀근
    • Microbiology and Biotechnology Letters
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    • v.31 no.4
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    • pp.377-382
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    • 2003
  • Purification of methioninase resulted in a yield of 69%, and SDS-PAGE analysis of the purified product revealed a single band of approximately 43 kDa in molecular weight. in vitro experiments with cancer cells incubated in methionine-free media demonstrated an increase in $^{11}$ C-methionine uptake to 25.8$\pm$1.1% at 6 hr, 31.8$\pm$0.8% at 24 hr, and 62.2$\pm$0.6% at 48hr, compared to controls. Treatment of the cancer cells with purified methioninase showed no decrease in survival after a 2 hr incubation with 0.01 U/ml, but survival of RR1022 cells decreased 30% after 24 to 48 hr incubation. SKOV-3 cells showed a 5% and 14% decrease in survival with 0.1 and 1 U/ml methioninase after 24 hr. After 48hr survival decreased 15% and 24% with 0.1 and 1 U/ml methioninase. Measurements of $^{11}$ C-methionine uptake in RR1022 cells demonstrated no change at 2 hr, but a 13.7$\pm$4.7% and 40.7$\pm$2.6% increase in uptake at 24 and 48 hr, respectively. SKOV-3 cells also showed no change at 2 hr, but had a 17.7$\pm$7.2% and 38.9$\pm$4.9% increase in $^{11}$ C-methionine uptake after 24 hr and 48 hr treatment with methioninase, respectively. $^{11}$ C-methionine PET imaging revealed clear visualization of both the tumors and contralateral infectious lesions. Administration of rMET appeared to result in a slight increase in tumor:nontumor contrast on $^{11}$ C-methionine PET images. Injection of purified methioninase also produced PET images where tumor uptake was higher than that of infectious lesions.

The Adsorption Mechanism of Copper (II) Ion on Acrylic Fiber Treated with Hydroxylamine (하이드록실 아민으로 처리한 아크릴섬유의 구리 (II)이온의 흡착기구)

  • Chin Young-gil;Choi Suk-chul
    • Journal of the Korean Society of Clothing and Textiles
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    • v.12 no.1 s.26
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    • pp.27-35
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    • 1988
  • In order to investigate a practical application of the fibrous adsorbent to heavy metal ions, acrylic fibers were treated with the hydroxylamine solution that was producted by hydroxylamine hydrochloride and potasium hydroxide in a condition of strong alkaline and $70^{\circ}C$. The adsorption mechanism of copper(2) ion on the fibrous adsorbent, that is hydroxylaminated acrylic fibers, was studied. The adsorption of copper(2) ion was explained in terms of the activated adsorption that are formed the complex with the ligand, such as C=N, N-H, NHOH, on the surface of the adsorbent. The activation energy was evaluated to be 3.8 Kcal/mol. and the times of adsorption equilibrium was approximately 10 minutes. The uptake of copper(2) ion was found to be effected with the increase of temperatures and the pH dependence.

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Preparation of Highly Tough Ethylene Vinyl Acetate (EVA) Heterogeneous Cation Exchange Membranes and Their Properties of Desalination

  • Kim, In Sik;Ko, Dae Young;Canlier, Ali;Hwang, Taek Sung
    • Korean Chemical Engineering Research
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    • v.56 no.3
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    • pp.361-369
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    • 2018
  • A manufacturing method has been devised to prepare novel heterogeneous cation exchange membranes by mixing ethylene vinyl acetate (EVA) copolymers with a commercial cation exchange resin. Optimum material characteristics, mixture ratios and manufacturing conditions have been worked out for achieving favorable membrane performance. Ion exchange capacity, electrical resistance, water uptake, swelling ratio and tensile strength properties were measured. SEM analysis was used to monitor morphology. Effects of vinyl acetate (VA) content, melt index (MI) and ion exchange resin content on properties of heterogeneous cation exchange membranes have been discussed. An application test was carried out by mounting a selected membrane in a membrane capacitive deionization (MCDI) system to investigate its desalination capability. 0.92 meq/g of ion exchange capacity, $8.7{\Omega}.cm^2$ of electrical resistance, $40kgf/cm^2$ of tensile strength, 19% of swelling ratio, 42% of water uptake, and 56.4% salt removal rate were achieved at best. VA content plays a leading role on the extent of physical properties and performance; however, MI is important for having uniform distribution of resin grains and achieving better ionic conductivity. Overall, manufacturing cost has been suppressed to 5-10% of that of homogeneous ion exchange membranes.

Control of Mg and P Ion Concentration as a Precondition to Use N, K and Ca Ion Sensors in Closed Hydroponics (N, K, Ca의 한정된 이온센서 이용을 전제로 한 순환식 수경재배에서 P, Mg의 조절 방법)

  • Choi, Gyeong Lee;Yeo, Kyung Hwan;Rhee, Han Cheol;Lee, Seong Chan;Lee, Jung-Sup;Kang, Nam Jun;Kim, Hak Jin;Jung, Dae Hyun
    • Horticultural Science & Technology
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    • v.34 no.6
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    • pp.871-877
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    • 2016
  • Recycling nutrient solutions in closed hydroponic production systems is usually accompanied by an imbalance of nutrient solutions when concentration is controlled according to electrical conductivity (EC) levels. This study investigated whether it was possible to automatically control the concentrations of five essential elements nitrogen (N), phosphorus (P), potassium (K), calcium (Ca) and magnesium (Mg) using only N, K and Ca ion sensors. N, P, K, Ca, and Mg uptake was measured in the nutrient solution, and relationships between absorbed ions were analyzed through twice-repeated experiments in lettuce. Results confirmed that the pattern of $PO_4$ ion uptake was similar that of N, and the pattern of Mg ion uptake was similar that of Ca. $PO_4$ ion uptake was most highly correlated with N, and Mg was most highly correlated with Ca. Regression coefficients of N and $PO_4$ were significantly different at 1.04 and 0.55, respectively, but were similar between Ca and Mg at 0.35 and 0.40, respectively. Additional experiments were conducted to measure nutrient uptake in pak choi and rose plants, both to confirm the results from the first experiment in lettuce, and to assess possible application to other crops. Coefficients of determination both for N and $PO_4$, and Ca and Mg were considerably high ($R^2=0.86$) in cultured pak choi, and similar results were observed in cultured rose ($R^2=0.87$ and 0.73, respectively). Regression coefficients for cultured pak choi were 0.56 and 0.24, respectively, and for rose were 0.51 and 0.16, respectively. Although the results obtained for N and $PO_4$ were not consistent between the lettuce experiments, N and $PO_4$ have similar regression coefficients for all crops. No common coefficient was found between Ca and Mg.

Lithium-induced Increase of Synaptosomal Uptake of Norepinephrine in Rat Brain

  • Cho, Young-Wuk;Han, Seung-Ho;Kim, Chang-Ju;Min, Byung-Il
    • The Korean Journal of Physiology and Pharmacology
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    • v.1 no.2
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    • pp.127-133
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    • 1997
  • Lithium remains the most widely used therapeutic agent for bipolar affective disorder, particularly mania. Although many investigators have studied the effects of lithium on abnormalities in monoamine neuro-transmitter as a pathophysiological basis of affective disorder, the action mechanism of lithium ion remains still unknown. To explore the action mechanism of lithium in the brain, we examined the effects of lithium on the extrasynaptosomal concentrations of catecholamines and their metabolites. Synaptosomes were prepared from the rat forebrains and assays of catecholamines and metabolites were made using HPLC with an electrochemical detector. Lithium of 1mM decreased the extrasynaptosomal concentrations of NE from the control group of $3.07{\pm}1.19$ to the treated group of $0.00{\pm}0.00$ (ng/ml of synaptosomal suspension) but not that of DHPG. It can be suggested that lithium increases synaptosomal uptake of NE. Increased intraneuronal uptake of NE would decrease neurotransmission and extraneuronal metabolism of NE. Because increased brain NE metabolism and neurotransmission have been suggested as important components in the pathophysiology of bipolar affective disorder, especially mania, lithium-induced increase of intraneuronal NE uptake can be suspected as an action mechanism of therapeutic effect of lithium in manic patient, possibly in bipolar affective disorder.

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Ion dependent cellular uptake of taurine in mouse osteoblast cell lines

  • Naomi Ishido;Emi Nakashima;Kang, Young-Sook
    • Proceedings of the Korean Society of Applied Pharmacology
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    • 2003.11a
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    • pp.109-109
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    • 2003
  • Taurine is present in a variety of tissue and exhibits many important physiological functions in many tissues. Although it is known that many tissues mediate taurine transport, its functions of taurine transport in bone have not been identified yet. In the present study, we investigated the expression of taurine transporter (TauT) and taurine uptake using mouse stromal ST2 cells and osteoblast-like MC3T3-El cells, which is bone related cells. Detection of TauT mRNA expression in these cells were performed by reverse transcription polymerase chain reaction (RT-PCR). The activity of TauT was assessed by measuring the uptake of [$^3$H]taurine in the presence or absence of inhibitors. TauT mRNA was detected in these cells. [$^3$H]Taurine uptake was dependent upon the presence of extracellular sodium, chloride and calcium ions, and inhibited by cold-taurine and ${\beta}$-alanine. These results suggest that taurine has biological functions in bone and some effect on the bone cells.

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Changes on the Methylmercury-induced Cytotoxicity by Control of Cell Membrane Transport System (세포막 물질수송계의 조절에 의한 유기수은의 세포독성 변화에 대한 연구)

  • 염정호;고대하;김준연;김남송
    • Journal of Environmental Health Sciences
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    • v.26 no.4
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    • pp.88-96
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    • 2000
  • The aim of the current study was to evaluate the transport system in EMT-6 cell for the uptake of the methylmercury(MeHg). Several inhibitors ere used to test used to test which potential transport system might be involoved in MeHg uptake. Probenecid was used to test the organic transport system, valinomycin for testing the effect of the membrane potential, cytochalasin B for testing the facilitated diffusive D-glucose transport system and colchicine for testing the microtubule system. Ouabain for evaluating active transport system, 4',4-diisothiocyano-2',2-stilbenedisulfonic acid(DIDS) the Cl- ion transport system and verapamil for the $Ca^{2+}$ transprot system. Significantly, MeHg decreased the synthesis of nitric oxcide(NO) and intracellular ATP in ENT-6 cells. In the condition of ouabain containing with MeHg decreased the production of NO and intracelluar ATP. In the treatment of inhibitors, ouabain showed protective effect against cytotoxicity of MeHg but ather inhibitors not showed protective effects. The protective effects of ouabain against the cytotoxicity of MeHg deoended on the concentration of added ouabain to the culture medium for MET-6 cells. These result showed that the uptake of MeHg might be involved in the active transport system. Active transports system seems to share similarities with the transport systems for the uptake of MeHg when using MeHg and MeHg-glutathione complex.x.

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