• Preventive Nutrition and Food Science
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• v.4 no.1
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• pp.79-83
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• 1999

A potentiometric biosensor employing a CO3-2 ion-selective electrode(ISE) and malic enzyme immobilization in al flow injection analysis (FIA) system was constructed. Analytical parameters were optimized for L-malate determination . The CO3-2 -ISE-FIA system was composed of a pump, an injector, a malic enzyme (EC1.1.1.40) reactor, a CO3-2 ion-selective electrode, a pH/mV meter and a recorder. Cofactor NADP was also injected with substrate for theenzyme reaction into the system. Optimized analytical parameters for L-malate determination in the CO3-2 ISE-FIA system were as follows ; flow rate, 14.5ml/hr ; sample injection volume, 100ul; enzyme loading in the reactor, 20 units ; length of the enzyme reactor , 7 cm ; tubing length form the enzyme reactor to the detector as a geometric factor in FIA, 15 cm . The response time for measuring the entire L-malate concentration range (10-2 ~10-5 mol/L ; 4 injections )was <15minutes . In this CO3-2 -ISE-FIA system, the potential differences due to th eformation of CO3-2 by the reaction of malic enzyme on L-malate were correlated to L-malate concentration in the range of 10-2 ~10-5mol/L ; the detection limit was 10-5 mol/L. This potentionmetric CO3-2 ISE--FIA system was found to be useful for L-malate measurement.

• Korean Journal of Clinical Laboratory Science
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• v.38 no.1
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• pp.59-64
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• 2006

The pH-ISE(ion selective electrode) based on tribenzylamine as a hydrogen ion carrier was prepared and its electrochemical characterization was studied. It responded linearly to hydrogen ions in the range of pH 3.1 - pH 11.0 and the Nernstian slope showed 55.0 mV/pH (at $20{\pm}0.2^{\circ}C$), it also showed a fast response time of 8 sec. When it was directly applied to human blood(pH 6.0-8.5), we could get the same satisfying results. A good reproducibility and stability were shown with the precision of 2 mV (${\pm}0.1$). The pH-ISE based on tribenzylamine exhibited biocompatibility in clinical applications.

• Bulletin of the Korean Chemical Society
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• v.22 no.7
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• pp.765-768
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• 2001

• Journal of the Korean Society of Safety
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• v.5 no.2
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• pp.40-49
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• 1990

For the determination of polluant NOx in ambient air, nitrate ion-selective electrode(ISE) was made. To comparison of NOx in each method, the nitrate-ISE, NEBA, Orion electrode were used to determinee NOx in ambient air. In this work, the concentration of NOx in ambient air was average 0.06ppm. The results were good agreement with those obtained by each method within a relative error of 3％, Absorbing efficiency of nitrogen oxides in ambient air was good for Alkali solution. The determination of nitrogen oxides in ambient air using the Aliquat 336N-PVC membrane electrode was one of the useful method. The best characteristics of the Aliquat 336N-PVC me,mbrane electrode were obtained with the ion-exchanger concentration level of 6.5-9.1 percent by weight. The optimal membrane composition, was 9.09wt.％ of ion-exchanger, 30.95wt.％ of PVC, 60.6wt.％ of plasticizer (DBP), and 0.5mm of thickness. Under the above condition, the electrode approached the Nernstian slope most closely, and the linear response ranges produced the best results.

• Journal of Biosystems Engineering
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• v.28 no.4
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• pp.335-342
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• 2003

A simple disposable thick-film potentiometric strip has been developed and evaluated for hydroponics application. The strip consisted of low ion-selective electrodes (ISE) fabricated by screen-printing technology. The electrochemical responses of ion sensors for nitrate, ammonium, potassium, and magnesium were measured with specially designed 16-channel low voltage signal transducers. The analytical characteristics of the sensors were comparable with those of conventional ISE sensors. The thick-film sensors exhibit linear relationships over five concentration decades. The concentration of N $O_3$ - ion in standard solution can be determined by direct potentiometric measurements without any conditioning before measurements. However, measurement of $K^{+}$, N $H_4$$^{+}$, and $Mg^{2+}$ ionic concentrations in nutrient solutions seems not feasible.

• Analytical Science and Technology
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• v.27 no.6
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• pp.333-338
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• 2014

A simple and sensitive analytical method for fluoride in urine by ion selective electrode (ISE) method was presented. Traditional buffer for fluoride determination using ISE is acetate-based one. Researchers have pointed out some drawbacks of the buffer for fluoride ISE analysis, and some other buffers including citrate-ammonium buffer and MES buffer have been studied for accurate determination of fluoride in urine here. These buffers provided promising results in environmental field, and this author focused on overcoming the interference of co-existing aluminium. The results show that MES-CyDTA buffer gave the best recovery with accuracy of 95-97.5% and precision of 1.9-7.9% for reference sample of 1.8-7.8 mg/L fluoride in urine, with smaller amount of samples and shorter analysis time compared with the traditional method which used acetate buffer. The method was applied to field samples, and which showed urinary of $0.98{\pm}0.38mg/g$ creatinine for workers in electric cable manufacturing factory (n=15) and $0.59{\pm}0.30mg/g$ creatinine for non-exposed workers (n=12).

• Preventive Nutrition and Food Science
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• v.3 no.1
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• pp.36-42
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• 1998

Ion-selective eleltrodes(ISEs) are simple electrodechemical devices for the direct measurement of ions in the samples. A novel potentiometric biosensor for the determination of L-Malate or D-isocitrate has been developed by using CO2-3 -ISE-FIA system was composed of a pump, an injector, a malic enzyme or isocitric dehydrogenase enzyme reactor, a CO2-3 -ISE, a pH/mV meter, and an integrater. The various factors, such as buffer capacity types of plstericizer and polymer, were optimized for the CO2-3 selectivity. In this novel CO2-3 --ISE-FIA system, the potential difference due to the amount of CO2-3 produced from each enzyme reaction was proportional to the amount of L-malate or D-isocitrate.

• YAKHAK HOEJI
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• v.46 no.5
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• pp.320-323
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• 2002

A method for the determination of acidic drug, mefenamic acid and ibuprofen with ion-selective electrode(ISE) using Fe(II)-di-2-pyridyl ketone oxime complex as a counter ion were developed. Benzyl-2-nitrophenyl ether(BNPE) plasticized membrane was more selective and sensitive than the other tested membranes. The acidic drug selective electrode exhibits a linear response for 10$^{-2}$ M 510$^{-5}$ M of acidic drugs, mefenamic acid and ibuprofen with a slope of -55.9 and -56.3 mV/dec. in borate buffer solution (pH 8.9). Potentiometric selectivity measurements revealed negligible interferences from aromatic and aliphatic carboxylic acid salts. The electrodes were found to be useful for the direct determination of mefenamic acid and ibuprofen in pharmaceutical preparations.

• Journal of Biosystems Engineering
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• v.41 no.2
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• pp.75-84
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• 2016

Purpose: In-situ water quality monitoring based on ion-selective electrodes (ISEs) is a promising technique because ISEs can be used directly in the medium to be tested, have a compact size, and are inexpensive. However, signal drift can be a major concern with on-line management systems because continuous immersion of the ISEs in water causes electrode degradation, affecting the stability, repeatability, and selectivity over time. In this study, a computer-based nitrate monitoring system including automatic electrode rinsing and calibration was developed to measure the nitrate concentration in water samples in real-time. Methods: The capabilities of two different types of poly(vinyl chloride) membrane-based ISEs, an electrode with a liquid filling and a carbon paste-based solid state electrode, were used in the monitoring system and evaluated on their sensitivities, selectivities, and durabilities. A feasibility test for the continuous detection of nitrate ions in water using the developed system was conducted using water samples obtained from various water sources. Results: Both prepared ISEs were capable of detecting low concentrations of nitrate in solution, i.e., 0.7 mg/L $NO_3-N$. Furthermore, the electrodes have the same order of selectivity for nitrate: $NO_3{^-}{\gg}HCO_3{^-}$ > $Cl^-$ > $H_2PO_4{^-}$ > $SO{_4}^{2-}$, and maintain their sensitivity by > 40 mV/decade over a period of 90 days. Conclusions: The use of an automated ISE-based nitrate measurement system that includes automatic electrode rinsing and two-point normalization proved to be feasible in measuring $NO_3-N$ in water samples obtained from different water sources. A one-to-one relationship between the levels of $NO_3-N$ measured with the ISEs and standard analytical instruments was obtained.

• Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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• v.21 no.1
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• pp.43-53
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• 2023

Sulfide concentrations critically affect worker safety and the integrities of underground facilities, such as deep geological repositories for spent nuclear fuel. Sulfide is highly sensitive to oxygen, which can oxidize sulfide to sulfate. This can hinder precise measurement of the sulfide concentration. Hence, a literature review was conducted, which revealed that two methods are commonly used: the methylene blue and sulfide ion-selective electrode (ISE) methods. Inductively coupled plasma optical emission spectroscopy (ICP-OES) was used for comparison with the two methods. The sulfide ISE method was found to be superior as it yielded results with a higher degree of accuracy and involved fewer procedures for quantification of the sulfide concentration in solution. ICP-OES results can be distorted significantly when sulfide is present in solution owing to the formation of H₂S gas in the ICP-OES nebulizer. Therefore, the ICP-OES must be used with caution when quantifying underground water to prevent any distortion in the measured results. The results also suggest important measures to avoid problems when using ICP-OES for site selection. Furthermore, the sulfide ISE method is useful in determining sulfide concentrations in the field to predict the lifetime of disposal canisters of spent nuclear fuel in deep geological repositories and other industries.