• Food Science of Animal Resources
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• v.42 no.2
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• pp.295-312
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• 2022

Inulin is a non-digestible carbohydrate and a prebiotic that can also act as a fat replacer in various foods. This study examined the effect of replacing vegetable oil with garlic inulin on the quality traits of chicken sausages. Water-based inulin gels were prepared using garlic inulin or commercial inulin to imitate fats in chicken sausages. Chicken sausages were prepared separately replacing vegetable oil with water-based inulin gels to reach final inulin percentages of 1, 2, and 3 (w/w). The control was prepared using 3% (w/w) vegetable oil with no inulin. The physicochemical properties and thiobarbituric acid reactive substance (TBARS) value of prepared sausages were analyzed over 28-d frozen storage. Sausages with 2% garlic inulin recorded higher flavour and overall acceptability scores (p<0.05). Ash, moisture, and protein contents of the sausages were increased with increasing levels of inulin while fat content was reduced from 13.67% (control) to 4.47%-4.85% (p<0.05) in 3% inulin-incorporated products. Sausages incorporated with 2% inulin had lower lightness (L^*) values than the control (p<0.05). Water holding capacity (WHC) was similar (p>0.05) among the samples. During storage L^* value, pH, and WHC decreased while redness (a^*) and yellowness (b^*) values increased in all the samples. In addition, TBARS values were increased during the storage in all samples within the acceptable limits. In conclusion, garlic inulin can be used successfully as a fat substitute in sausages without altering meat quality parameters.

• Korean Journal of Environmental Agriculture
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• v.39 no.3
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• pp.188-196
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• 2020

BACKGROUND: In order to cultivate upland crops in reclaimed land, economically appropriate crops should be selected. Jerusalem artichoke (Helianthus tuberosus L.) is one of the primary sources for inulin in higher plants. The objective of this study was to evaluate and compare growth and inulin of H. tuberosus L. according to the cultivation region in Korea. METHODS AND RESULTS: 21 varieties of H. tuberosus L. were collected in Korea, and then 7 varieties of H. tuberosus L. were selected for this study. To compare growth and inulin, those varieties were cultivated in reclaimed land and upland soils. The growth of H. tuberosus L. significantly decreased when cultivated in reclaimed land. There was an increas at 17.8% in the content of white H. tuberosus L. tuber inulin in reclaimed land. CONCLUSION: The content of inulin in H. tuberosus L. tubers was found to be dependent on cultivation region and tuber color. There was a difference of the inulin content between the tubers with different colors in reclaimed land. Purple tubers of P20 were considered suitable varieties for cultivation in reclaimed land. White tubers of W1 and W8 were also considered suitable varieties for cultivation in reclaimed land.

• Microbiology and Biotechnology Letters
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• v.19 no.2
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• pp.158-162
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• 1991

Endoinulinase was purified from a commercial inulin preparation produced by Aspergillus ficuum using ion exchange chromatography on CM-Sephadex C-50 and DEAE-Sepharose 6B, HPLC gel filtration on a Protein Pak 125 Colum and HPLC ion exchange chromatography on a TSK DEAE-5pw Column. The endoinulinase had a molecular weight of 72,000${\pm}$1,000 and was glycoprotein with 23 to 25% w/w sugar content. The enzyme was much more active on inulin with random cleavage mode than on sucrose and on palatinose: The ration of activity on inulin and sucrose (I/S ratio) was 10~14.

• Journal of Microbiology and Biotechnology
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• v.29 no.11
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• pp.1777-1789
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• 2019

Drought is more concerned to be a huge problem for agriculture as it affects plant growth and yield. Endophytic bacteria act as plant growth promoting bacteria that have roles for improving plant growth under stress conditions. The properties of four strains of endophytic bacteria were determined under water deficit medium with 20% polyethylene glycol. Bacillus aquimaris strain 3.13 showed high 1-aminocyclopropane-1-carboxylate (ACC) deaminase production; Micrococcus luteus strain 4.43 produced indole acetic acid (IAA). Exopolysaccharide production was high in Bacillus methylotrophicus strain 5.18 while Bacillus sp. strain 5.2 did not show major properties for drought response. Inoculation of endophytic bacteria into plants, strain 3.13 and 4.43 increased height, shoot and root weight, root length, root diameter, root volume, root area and root surface of Jerusalem artichoke grown under water limitation, clearly shown in water supply at 1/3 of available water. These increases were caused by bacteria ACC deaminase and IAA production; moreover, strain 4.43 boosted leaf area and chlorophyll levels, leading to increased photosynthesis under drought at 60 days of planting. The harvest index was high in the treatment with strain 4.43 and 3.13 under 1/3 of available water, promoting tuber numbers and tuber weight. Inulin content was unchanged in the control between well-watered and drought conditions. In comparison, inulin levels were higher in the endophytic bacteria treatment under both conditions, although yields dipped under drought. Thus, the endophytic bacteria promoted in plant growth and yield under drought; they had outstanding function in the enhancement of inulin content under well-watered condition.

• Nutritional Sciences
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• v.7 no.4
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• pp.196-200
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• 2004

There are increasing evidences that prebiotics can modulate various properties of the immune system. This study was conducted to investigate effects of three kinds of fructans (chicory inulin, chicory inulin oligosaccharide and fructooligosaccharide) and a glucose oligomer(isomaltooligosaccharide) in large bowel mass and innnunoglobulin A (IgA) in rats. Forty five Sprague-Dawley male rats weighing about 1909 were randomly sorted to receive one of the five treatments, which were control diet, control diet+6% isomaltooligosaccharide (IMOS), control diet+6% fructooligosaccharide (FOS), control diet+6% chicory inulin oligosaccharide (CIOS), or control diet + 6% chicory inulin (CI). Rats were pair-fed and received the experimental diets for 5 weeks. Cecal and colonic wall weights were significantly higher in fructan (FOS, CIOS, CI)-fed groups compared with control and IMOS groups, and the length of colon was elevated in FOS and CIOS groups compared with control group. Fecal concentrations of acetic acid and total short-chain fatty acids (SCFAs) were significantly elevated in fructan-fed groups. Plasma and cecal levels and fecal excretion of immunogiobulin A (IgA) in rats were not significantly different among groups. However, fructooligosaccharide tended to increase IgA level in cecum. Cecal IgA level was significantly negatively correlated with pH of cecal content (r=-0.337), positively correlated with acetic acid level (r=0.310). Fecal IgA excretion was positively correlated with total SCFA (r=0.311) and propionic acid (r=0.400) level in feces. These results indicate that fructooligosaccharide and chicory inulin oligosaccharide exerted trophic effects in large bowel wall, increased production of SCFAs and decreased pH, which were conditions positively associated with cecal and colonic IgA secretion.

• Animal Bioscience
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• v.35 no.8
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• pp.1250-1257
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• 2022

Objective: Low fat duck meat sausages were prepared by replacing the fat in the formulations with soy protein isolate (SPI) and inulin to find the best formulation having superior shelf-life without affecting its quality attributes. Methods: Four sausage mix formulations were prepared viz.control (0% SPI and inulin), T₁ (2.5% inulin), T₂ (2.5% SPI), and T₃ (2.5% SPI+2.5% inulin) replacing duck fat as per the recipe. Five batches of duck meat sausages of each formulation were prepared, and the final products were evaluated for physico-chemical, organoleptic, and microbiological qualities. Results: The % moisture and crude protein content of the sausages revealed an increasing trend (p<0.01) from control to the treated formulations, while the % total ash contents were found to be non-significant (p>0.05). On the contrary, the per cent ether extract decreased significantly (p<0.01) from the control to the treated groups. In terms of calorie value, control samples exhibited the highest values with a significant (p<0.01) regression from control to treated formulation, respectively. The colour profile study (L, a^*, b^*) of the formulations were found to be non-significant. Texture profile study in terms of springiness, cohesiveness, chewiness, and resilience revealed no significant difference in all the treatment groups except the hardness scores, which revealed a significantly (p<0.01) increasing trend from control to the treated formulations. The total viable count showed a significant decrease in the treated groups. However, there was a significant increase in the bacterial load during the storage till day 15th. The total viable psychrophilic bacterial count showed a significant (p<0.01) increase in bacterial load from day 5th to 15th day of storage. Colititre counts were negative for all the formulations until the 15th day of storage. Conclusion: The present study results may conclude that duck meat sausages could be prepared satisfactorily by replacing duck fat with SPI and inulin at the rate of 2.5% of each with superior quality attributes.

• Microbiology and Biotechnology Letters
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• v.16 no.6
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• pp.484-488
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• 1988

Two forms of extracellular endo-inulinase, designated as PIand P II were resolved from a species of Pseudomonas isolated from soil. Both enzymes were glycoproteins with their carbohydrate content of 15% for PIand 2.4% for P II inulinase. Tryptophan residue was proved to be an essential amino acid for their catalytic activity. The molecular weights of PIand P II were estimated to be 210, 000 and 170, 000, respectively. The activity of the two enzymes was strongly inhibited by p-chloromercuribenzoate but the inhibition was nearly completely offset by the addition of the reducing agents such as cysteine or dithiothreitol. On the other hand, the two enzymes were activated about 50-60% of their activities by the presence of Co$^{+2}$ ion, and quite stable at pH values ranging from pH 4.0 to 1.5. They also appeared to be relatively thermostable, and no appreciable inactivation was observed after incubation at 55$^{\circ}C$ for 2 hours. About 70 % hydrolysis rate with PIand 56 % with P II were achieved when inulin was hydrolyzed at 5$0^{\circ}C$ for 12 hours with 60 units of the enzymes in 2 % inulin solution.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.223-223
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• 2017

This study aimed to determine the effect of organic matter content and water condition in soil on yield, carbohydrate, and inulin contents of two varieties of Jerusalem artichoke. The plants were grown with high and low organic soil either under irrigation or no irrigation in a randomized complete block design (RCBD) with three replications. In result, the yield of Jerusalem artichoke was higher about 7 kg in soil with high organic matter than in soil with low organic matter, while it was higher about 11 kg under the irrigation than under no irrigation. The yield of white variety was higher about 9 kg than that of purple variety. Under the irrigation, a 12 kg and 9 kg of higher yields were observed in the soil with high and low organic matter content, respectively, than under no irrigation.

• Food Science and Preservation
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• v.30 no.5
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• pp.743-757
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• 2023

The formulation of a novel functional fresh apple juice enriched with dietary prebiotic fiber (inulin or polydextrose), ginger extract (GE), and cardamom essential oil (CEO) was carried out based on a combined D-optimal design. In the first stage, sensory evaluation was performed to screen and select the optimum sample for further experiments. The sensory evaluation showed that the sample containing inulin 0.25 g/100 g GE and 0.03 g/100 g CEO had the highest organoleptic score. In the second stage, various chemical experiments, including pH, acidity, formalin index, total phenol, flavonoids, antioxidant capacity, and vitamin C content, were evaluated on the selected enriched apple juices. The addition of GE and CEO caused changes in nutritional characteristics, including antioxidant capacity, total phenol, flavonoids, vitamin C, and IC50, from 35 g/100 g, 350 mg GAE/g, 17 mg/L, 370 mg/kg, and 1,800 mg/kg to 45 g/100 g, 460 mg GAE/g, 21 mg/L, 420 mg/kg, and 1,200 mg/kg respectively. The steady shear flow and dynamic oscillatory shear rheological tests were also performed on the screened samples, and results showed that the addition of dietary fiber in apple juices increased the apparent viscosity, storage modulus, loss modulus, and complex viscosity. In general, adding plant extracts and processed essential oil to apple juice increased the nutritional-nutraceutical value and sensory attributes of apple juice.

• Microbiology and Biotechnology Letters
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• v.50 no.3
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• pp.347-353
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• 2022

In this study, the effect of a synbiotic mixture of probiotics and 3% inulin on the growth, non-specific immune response, and mortality due to disease resistance of Paralichthys olivaceus was investigated. The results revealed that an 8-week diet of synbiotic-added feed, had no significant effect on the growth of P. olivaceus, Analysis of the hematological parameters revealed that there was a significant difference in the content of AST, total protein, and cholesterol among the groups, whereas there was no significant difference in the ALT and glucose contents. Further, there was no significant difference in the lysozyme activity of the synbiotic and control group after 8 weeks' feeding. In addition, when the symbiotic mixture was used rather than probiotics alone, the NBT activity of the synbiotic group increased compared to that of the control group. Moreover, when only probiotics were used, the cytokine expression in the spleen of the samples was significantly lower than that in the control group, whereas the expression was significantly higher in the spleen of the synbiotics group. However, there was no significant difference in the cytokine expression in the liver, intestine, and kidney of the three groups. Lastly, after injection for 2 weeks, the mortality rates of the control group towards Edwardsiella tarda, Streptococcus parauberis, and Streptococcus iniae were 95, 85 and 85%, respectively; those of the probiotics group were 85, 80, and 80%, respectively; and those of the synbiotics group were 80, 80, and 85%, respectively.