• Korean Journal of Poultry Science
• /
• v.26 no.4
• /
• pp.253-259
• /
• 1999

The objects of this experiment was to investigate the effect of dietary several supplemental probiotics on performance and intestinal microflora of Lohmann brown laying hens from 68 to 80 weeks. Basal diets based on corn and soybean meal contained 18.0% CP and 2,720㎉/kg ME. Bacillus subtilis, Lactobacillus salvarius isolated from piglet(LSP) were fed at the level of 0.1 and 0.2% in a one way design. There were four replicates of 40 hens each per treatment. Egg production, feed intake, feed conversion ratio(FCR), eggshell quality were measured at every four weeks and intestinal microflora were examined at the end of experiment. Egg production of bird fed 0.2% individual probiotics was significantly higher than that of control(P 0.05). Birds fed the diet containing 0.2% LSC and LSP had significantly lower FCR than other treatments(P 0.05). However, egg weight of birds fed control and 0.2% BS diet showed higher than other treatments. Feed intake of 0.2% BS and 0.1% LSP treatment was significantly higher than other treatments, but was not consistency of all treatments(P 0.05). Eggshell breaking strength and thickness of hens fed probiotics tended to increase compared to that of control, but was not significantly different. Intestinal anaerobes, Lactobacillus spp. and yeast of hens fed all tested probiotics were significantly increased compared to those of control. The number of intestinal E. coli of all probiotics treatments except 0.1% LSP tended to decrease. Intestinal Lactobacillus spp. was increased significantly by 0.1% dietary LSC, whereas intestinal yeast showed significant increase in LSP treatments(P〈0.05). The results of this experiment indicated that feeding probiotics to laying hens improved the egg production, FCR and increased beneficial microflora.

• Asian-Australasian Journal of Animal Sciences
• /
• v.16 no.11
• /
• pp.1673-1679
• /
• 2003

Two hundred forty 1-d-old Arbor Acres broiler chicks were used to investigate the effects of Cu (II)-exchanged montmorillonite (CEM) or montmorillonite on the growth performance, intestinal microflora, bacterial enzyme activities and morphology of broilers. The chicks were assigned randomly into three groups with 80 chicks per treatment. The three dietary treatments were basal diet only (control group), basal diet +1 g $kg^{-1}$ montmorillonite, and basal diet +1 g $kg^{-1}$ CEM. The results showed that the addition of CEM to the diet increased significantly the body weight and feed efficiency, but a similarly significant increase was not found in broilers fed the diet containing montmorillonite. Supplementing the CEM in the diet of broilers also decreased the numbers of Clostridium perfringens and Escherichia coli in the small intestine and cecum. The addition of either CEM or montmorillonite to the diet depressed the activities of $\beta$-glucosidase and $\beta$-glucuronidase in the small intestinal and cecal contents. Data of villus height and crypt depth for duodenum, jejunum and ileum indicated that dietary addition of CEM or montmorillonite improved the small intestinal mucosal morphology.

• Journal of Microbiology
• /
• v.39 no.3
• /
• pp.154-161
• /
• 2001

In recent years, colon cancer has been reported to be one of the most important causes of cancer morbidity and mortality in Korea. Epidemiological and experimental studies suggest that lactic acid bacteria (LAB) used to ferment dairy products inhibits colon carcinogenesis. The present study was designed to determine whether the colon cancer inhibitory effect of LAB (Bifidobacterium longum Hy8001; Bif and Lactobacillus acidophilus HY2l04; Lac) of Korean origin, is associated with intestinal microflora composition and certain enzyme activity in rats treated with azoxymethane (AOM). At five weeks of age, SD rats were divided at random into four (AOM alone, Bif, Lac, and Bif+Lac) groups. Oral administration of lactic acid bacteria cultures were performed daily until the termination of the study. Two weeks later all animals were given a subcutaneous injection of AOM dissolved in normal saline at a dose of 15 mg/kg of body weight once weekly for 2 weeks. Every two weeks for 10 weeks, five of the rats in each group were randomly chosen for fecal specimen collection. The fecal specimens were used for assay of $\beta$-glucuronidase and nitroreductase, and analysis of intestinal microflora composition. The activity of $\beta$-glucuronidase which plays an important role in the production of the carcinogenic metabolite of azoxymethane was remarkably increased in the AOM alone group after AOM injection and maintained the high level during the experiment. However, LAB inhibited the AOM-induced increase in $\beta$-glucuronidase activity. Nitroreductase activity decreased by 30-40% in LAB treated groups in comparison with that of the AOM alone group. The results of the present study suggest that LAB inhibits colon carcinogenesis by modulating the metabolic activity of intestinal micro-flora and improving the composition of intestinal microflora.

• Animal Bioscience
• /
• v.35 no.11
• /
• pp.1689-1697
• /
• 2022

Objective: The aim of this study was to compare the effects of three kinds of organic acid (OA) products on the growth performance, intestinal characteristics and morphology, and cecal microflora in broilers fed a corn-soybean meal meal diet. Methods: A total of 420 one-day-old male Cobb 500 broilers with an average initial body weight of 49.11±1.02 g were used in this 42-day experiment. Birds were randomly allotted to one of five treatments (7 replicates with 12 birds per replicate). Treatments consisted of negative control (NC), basal diet; positive control (PC), basal diet+100 mg/kg of Aviramycin; OA1, basal diet+500 mg/kg of OA product 1; OA2, basal diet+1,000 mg/kg of OA product 2; and OA3, basal diet+1,200 mg/kg of OA product 3. Results: The results indicated that OA product addition had no effect on growth performance parameters, such as body weight gain, feed intake, and feed conversion ratio, from days 1 to 14, 15 to 28, and 0 to 42, or on the pH values of the intestine, intestinal weight, or intestinal weight to body weight ratio. The intestinal morphology in terms of villus height and crypt depth were affected by dietary supplementation of OA products, respectively. Furthermore, dietary addition of OAs had positive influences on the maintenance of the cecal microflora based on the results of 16S rRNA analysis. Conclusion: Dietary inclusion of three kinds of OA products all benefit broilers, but the mode of action may be different. This study provides a basis for the application of OA products used in the poultry industry.

• Korean Journal of Veterinary Research
• /
• v.34 no.1
• /
• pp.107-113
• /
• 1994

The present study was undertaken to obtain the basic knowledge on the distribution of intestinal microflora and characterization of Bifidobacterium isolated from the intestine of cattle, pigs, chicken and dogs. Bacteroidaceae and Streptococcus were isolated from the feces of cattle as predominant organisms, and Bifidobacterium was in counts of $8.65{\pm}0.21$ log 10 organisms. Bacteroidaceae, Eubacterium and Peptococcaceae counts on the feces of pigs were particularly high, but Bifidobacterium did not isolated. In hens and dogs, the counts of Bifidobacterium were $7.60{\pm}0.71$ and $8.15{\pm}2.04$ log 10 organism, respectively. Isolated 7 Bifidobacterial strains were identified respectively to B. thermophilum from cattle, B. pullorum and B. animalis from pigs, and B. longum, B. adolescentis and B. pseudolongum from dogs by their carbohydrate fermentation ability.

• Microbiology and Biotechnology Letters
• /
• v.23 no.6
• /
• pp.633-640
• /
• 1995

As a result of screening the medicinal herbs which selectively control human intestinal microflora, water extract of Akebia quinata Decaisne was proved to have a strong inhibitory activity against the growth of Clostridium pefringens, a major harmful intestinal bacterium. The anti-bacte-rial activity was stable under the thermal treatment at 100$\circ$C for 120 min and in a range of pH 1 to 11. In addition, the water extract of Akebia quinata Decaisne showed the antibacterial activities against five different strains of Clostridia including C. perfringens. On the contrary, the extract did not inhibit the growths of Bifidobacterium, Lactobacillus, Escherichia coli, Enterococcus faecalis. The extract, however, suppressed markedly the growth of Bacteroides fragilis and Staphylococcus aureus in vitro. Alike in the mixed culture inoculated with human feces as starter, in vivo tests using rats showed that the extract tends to increase the numbers of Bifidobacteria and Lactobacilli in the intestinal microflora of rats, whereas those of Clostridia were attenuated.

• Asian-Australasian Journal of Animal Sciences
• /
• v.24 no.1
• /
• pp.96-102
• /
• 2011

In this study, we examined the effects of formic acid administration to the drinking water on performance, intestinal microflora and carcass contamination in male broilers. A total of 312 day-old male broiler chicks were allocated to two groups with three replicates. The first group (control) received normal drinking water (pH 7.4) during the experiment. The second group consumed acidified drinking water (pH 4.5) after 5 d of age. At 43 d of age, twelve birds were randomly selected from the control group to determine the effect of acidified drinking water on carcass contamination. These birds were only given normal or acidified (pH 3) drinking water for 8 h prior to slaughter. The reduction of water pH from 7.4 to 4.5 significantly decreased body weights of male broilers at 21 and 42 d of age. However, no differences were observed between male broilers given normal and acidified drinking water in terms of feed intake, feed conversion ratio and mortality. The pH value of the gizzard contents was not significantly affected by acid water treatment. There were no significant differences in the intestinal population of E. coli, total organism and Salmonella between the groups. The total organism and E. coli counts of the carcass slightly decreased in the acidified group. No Salmonella was identified in carcass samples of any of the treatment groups. The results showed that drinking water acidification did not provide beneficial effects on performance, intestinal microflora and carcass contamination in male broilers.

• Korean Journal of Food Science and Technology
• /
• v.33 no.2
• /
• pp.252-255
• /
• 2001

This study was performed to investigate the influence of dietary mulberry leaf on the intestinal microflora in rats. Rats were fed each experimental diets containing 1%, 10% of mulberry leaf powder for 4 weeks. Total viable counts and the numbers of Bifidobacterium, Lactobacillus, Clostridium, E. coli and Staphylococcus were determined by nonselective media and various selective media. A decrease in the intestinal population of Clostridium was shown in dietary mulberry leaf group. The E. coli and Staphylococcus populations decreased in dietary mulberry leaf group compared with control group. Methanol extract and fractions of mulberry leaf were subjected to an in vitro screening test for their growth-inhibitory activity. Methanol extract and Water fraction of Mulberry leaves showed weak growth-inhibition of Clostridium perfringens. These results indicate that the composition of gastrointestinal microflora was improved by treatment of mulberry leaves in SD rats and was very effective for growth inhibition of the intestinal harmful bacteria in intestine. Therefore, the mulberry leaves as a newly bio-material can be a useful material for physiologically functional food.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.39 no.11
• /
• pp.1587-1594
• /
• 2010

This study was designed to investigate the effect of Artemisia capillaris extracts on the intestinal microflora. In agar diffusion method, the solvent fractions of Artemisia capillaris showed growth inhibition against the intestinal microflora. In particular, the chloroform fraction of Artemisia capillaris had strong antibacterial activity against Clostridium perfringens, Clostridium difficile, Eubacterium limosum, and Bacteroides fragilis, but did not show antibacterial activity against Bifidobacterium bifidum and Lactobacillus acidophilus. Most chloroform fraction of Artemisia capillaris inhibitory activities were not reduced by heat treatment or pH variation against C. perfringens, C. difficile, E. limosum, and B. fragilis. MICs of the chloroform fraction were 1.25 mg/mL against C. perfringens, E. limosum and B. fragilis and 2.5 mg/mL against C. difficile. MBCs of chloroform fraction were 5 mg/mL against C. perfringens, E. limosum and 2.5 mg/mL against C. difficile, B. fragilis. The ethyl acetate fraction of Artemisia capillaris showed $3.08{\pm}0.03$ mg/10 mg total polyphenol and $1.91{\pm}0.03$ mg/10 mg total flavonoid contents. In vivo tests were performed to investigate the influence of Artemisia capillaris extract on the intestinal microflora in rats. The results showed the possibilities of utilizing Artemisia capillaris extracts as a functional food component to control intestinal microflora.

• Journal of Ginseng Research
• /
• v.41 no.4
• /
• pp.540-547
• /
• 2017

Background: In general, after Panax ginseng is administered orally, intestinal microbes play a crucial role in its degradation and metabolization process. Studies on the metabolism of P. ginseng by microflora are important for obtaining a better understanding of their biological effects. Methods: In vitro biotransformation of P. ginseng extract by rat intestinal microflora was investigated at $37^{\circ}C$ for 24 h, and the simultaneous determination of the metabolites and metabolic profile of P. ginseng saponins by rat intestinal microflora was achieved using LC-MS/MS. Results: A total of seven ginsenosides were detected in the P. ginseng extract, including ginsenosides Rg1, Re, Rf, Rb1, Rc, Rb2, and Rd. In the transformed P. ginseng samples, considerable amounts of deglycosylated metabolite compound K and Rh1 were detected. In addition, minimal amounts of deglycosylated metabolites (ginsenosides Rg2, F1, F2, Rg3, and protopanaxatriol-type ginsenosides) and untransformed ginsenosides Re, Rg1, and Rd were detected at 24 h. The results indicated that the primary metabolites are compound K and Rh1, and the protopanaxadiol-type ginsenosides were more easily metabolized than protopanaxatriol-type ginsenosides. Conclusion: This is the first report of the identification and quantification of the metabolism and metabolic profile of P. ginseng extract in rat intestinal microflora using LC-MS/MS. The current study provided new insights for studying the metabolism and active metabolites of P. ginseng.