• IMMUNE NETWORK
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• v.12 no.4
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• pp.129-138
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• 2012

Allergic disorders such as atopic dermatitis and asthma are common hyper-immune disorders in industrialized countries. Along with genetic association, environmental factors and gut microbiota have been suggested as major triggering factors for the development of atopic dermatitis. Numerous studies support the association of hygiene hypothesis in allergic immune disorders that a lack of early childhood exposure to diverse microorganism increases susceptibility to allergic diseases. Among the symbiotic microorganisms (e.g. gut flora or probiotics), probiotics confer health benefits through multiple action mechanisms including modification of immune response in gut associated lymphoid tissue (GALT). Although many human clinical trials and mouse studies demonstrated the beneficial effects of probiotics in diverse immune disorders, this effect is strain specific and needs to apply specific probiotics for specific allergic diseases. Herein, we briefly review the diverse functions and regulation mechanisms of probiotics in diverse disorders.

• The Journal of Korean Academy of Orthopedic Manual Physical Therapy
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• v.5 no.1
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• pp.17-26
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• 1999

The pelvic floor is a muscular structure, pierced by urologic, genital, and distal intestinal tract. Also pelvic floor is not a frozen but a functional unit. The pelvic floor dysfunction has 1) laxity of soft tissue and muscle 2)rupture of pelvic floor, 3)increased the tension. The purpose of this study is to give information about the pelvic floor dysfunction and pelvic exercise. This investigate the pelvic floor structure and function, pelvic floor dysfunction, pelvic floor exercise, and recent research trends. The pelvic floor exercise is one of important exercise in physical therapy, this exercise program will be improved patients with pelvic floor dysfunction.

• Korean Journal of Microbiology
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• v.16 no.4
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• pp.161-169
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• 1978

Escherichiae-like organisms were isolated from rectal specimens of 56 children who were either in preschool age or in elementary school. The isolated strains were subjected to tests to screen enteropathogens producing heat-labile enterotoxin and susceptibility test to various antibiotics by disc diffusion method on agar plates. Production of heat-labile enterotoxin by the strains was assyed in the sensitive and reproducible cultured adrenal tumor cell system. The assay was sterodogenesis of the cell in the presence of heat-labile enterotoxin. Among 56 strains, gave positive reaction in the test of toxin production. This meant that about 10% of the children population objected to the study harbored the toxigenic strain of enteropathogenes. Some of these toxigenic strains were resistant to the antibiotics employed in the test. This study suggested that considerable population in Korea may harbor entertoxigenic E. coli as a part of intestinal normal flora. The toxigenic strains which are resistant to antibiotics may bring issue of public health in the future.

• Journal of environmental and Sanitary engineering
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• v.2 no.2 s.2
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• pp.61-67
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• 1987

The genus Vibrio contains some of the most important intestinal pathogens of humans, including Vibrio cholerae, the cause of epidemic Asiatic cholera. A group of organisms which have been reffered to as the non-agglutinating vibrio (NAG) do not agglutinate in the Vibrio cholerae 0 group 1 antisera, but are indistinguishable from the 0-1 group both chemically and genetically. Non-O-l Vibrio cholerae can cause isolated as well as focal outbreaks of diarrhea, but the volume of fluid loss does not approach that of classic cholera, and the disease is usually self-limiting. These free-living organisms are found world-widely distributed in the environment including sewage, contaminated water, estuaries, seafood and animals. These strains involved in several cases were isolated from the environment and some patients of diarrhea, and a few epidemiologic reports indicated the wide distribution of the strains throughout the country, giving an attention to the role the organisms may play in an outbreak of diarrhea in Korea. More research on the epidemiology, serologic typing and virulence of the group of organisms, should be, therefore, done to obtain a complete understanding of their role in human disease.

• Journal of Applied Biological Chemistry
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• v.44 no.2
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• pp.77-83
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• 2001

For defining the possibilities of the commercial mass liquid culture of Cordyceps sinensis, the pharmacological activities of mycelia were analyzed. The mycelium of C. sinensis consists of carbohydrate (5.1%) and fat (1.3%), and contains a low content of protein (0.7%) and ash (0.5%), and 92.4% moisture. The molecular sugar ratio of carbohydrate was composed mainly of glucose, mannose (1.0 : 0.9), in addition a small amount of galactose and arabinose (0.2 : 0.1). The cellular materials of mycelia were fractionated into ethylacetate (EA), MeOH (M) and hot-water extract fraction (HW). HW fraction showed the most potent intestinal immune system modulating activity, anti-coagulant activity, and anti-complementary activity, and M fraction had the inhibition activity of radical generation as effective as genistine. These results reveal that the mycelium of liquid cultured C. sinensis showed pharmacological activities and could be used for commercial purpose.

• Journal of Applied Biological Chemistry
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• v.63 no.1
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• pp.35-41
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• 2020

(-)-epigallocatechin-3-gallate (EGGG), a flavonoid found in green tea, is known to have low bioavailability. In this study, we determine whether piperine, a natural bioenhancer, can increase the absorption rate of EGCG. Using a Caco-2 cell monolayer, permeability experiments were performed in Hanks' balanced salt solution (HBSS) and EGCG stability was adjusted to pH 6.5 and pH 5.5 by ascorbic acid treatment. When HBSS was adjusted to pH 6.5, EGCG remained at 94.78% for up to 2 h and remained at 86.04% after 4 h and the net efflux decreased compared to the control. As a result, uptake was significantly increased in the piperine co-administered group compared to the EGCG-alone group, showing that piperine increased the permeability of EGCG in the Caco-2 cell monolayer. These results suggest that piperine inhibits EGCG glucuronidation and efflux, allowing for greater absorption of EGCG.

• Journal of Food Hygiene and Safety
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• v.7 no.4
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• pp.173-176
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• 1992

Non-absorbable substances in foods, for instance dietary fiber had been previously known as a non-nutritive part of foods. Recently , such a category has been gradually changed to as one of nutrients, As a main reason, dietary fibers includes many poly-or oligo-saccharides, which as resistant to alimentary hydrolyzing enzyme, However, parts of them are fermented by intestinal micro-organism to produce short chain fatty acids and so on. They are absorbed and utilized by human being. Now, it may be naturally accepted that dietary fiber is a kind of nutrients. Dietary fiber exerts many useful functions on body. They are classified into three large function , physicochemical function, physiological function and biological function. The last function of dietary fiber will be presented in the symphosium. Dietary fiber has several kinds of nutritional properties. One is energy source. Short chain fatty acids(SCFA) are oxidized and produced energy in body. Dietary fiber has not high energy, but not zero kilocalories. Another one is to be a constitutional component of higher animals' tissue. Last but most important one is physiological functions of dietary fiber.

• 한국방재학회:학술대회논문집
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• 2008.02a
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• pp.831-833
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• 2008

Intestinal pathogenic bacteria, Campylobacter, were detected in water samples collected from the Yeong-san river. 50 ml of water samples were filtered and incubated in enrichment broth. PCR using campylobacter genus specific primers showed positive results in all sites. We report the epidemical potential public health risk.

• Journal of Nutrition and Health
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• v.32 no.2
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• pp.129-136
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• 1999

This study was conducted to investigate the effect of fructooligosaccharide on intestinal flora, lipid metabolism and immune response. Thirty two male rats of Sprague-Dawley strain were divided into two groups according to body weight. Each group was fed the diet containing 2% cholesterol or the normal diet, respectively for 4 weeks. Each group was again divided into two sub-groups and they were fed with the diet containing 5% of sucrose and fructooligosaccharide, respectively for 8 weeks. The number of bifidobacteria slight increased, but not significantly, in oligosaccharide groups. Plasma total lipid concentration in cholesterol group was signficantly increased compared to the value in normal group. Dietary oligosaccharide decreased plasma total lipid concentration and triglyceride concentrations in normal group, but not in cholesterol group. Fecal lipid excretion was higher in cholesterol group than in normal group. Fecal cholesterol concentration in cholesterol-oligosaccharide group was significantly increased compared to other groups. Cholesterol had slight effects on TBARS and the immune status, but dietary oligosaccharide had no effects on these variables. The results in this study suggest that dietary oligosaccharide had no effect on serum and liver profiles of rats fed high cholesterol diet but increases the fecal cholesterol excretion.

• Journal of Dairy Science and Biotechnology
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• v.37 no.1
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• pp.15-26
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• 2019

A common belief is that human milk is sterile. However, the development of culture-independent molecular methods, especially Next Generation Sequencing, has revealed that human milk harbors diverse and rich bacterial communities. Although studies aimed at characterizing the microbiota of human milk have produced different findings, Staphylococcus and Streptococcus are presumed to be normal members of the microbiota. Factors that influence variation in the microbiota are unclear; however, the postpartum time, route of delivery, maternal obesity, and health status may be influential. The origin of the microbiota is a hotly debated topic. Human milk bacteria are thought to be introduced through bacterial exposure of the mammary duct during breast feeding and/or the entero-mammary pathway from the maternal gastrointestinal tract. Although the exact mechanism related to the entero-mammary pathway is unknown, it is presumed that bacteria penetrate the intestinal epithelium and then migrate to the mammary gland, dendritic cells, and macrophages. In this review, various relevant studies are introduced.