• 식물분류학회지
• /
• 제52권4호
• /
• pp.219-225
• /
• 2022

To elucidate the ancestry of the allopolyploids E. stevenii and E. boöphthona, I examined eleven isozyme loci and 24 morphological characters from 28 populations representing five related Euphorbia species from Australia. According to an analysis of genetic and morphological data, three diploid species differentiated recently, but two independent polyploid species are estimated to have differentiated a relatively long time ago. Fixed heterozygosity for most isozymes in E. stevenii and E. boöphthona strongly suggests that these two species are allopolyploids rather than autopolyploids. The isozyme profiles of E. stevenii indicate that it is an allopolyploid that evolved from interspecific hybridization between the diploid E. tannensis and unidentified or extinct tetraploid species. In addition, isozyme patterns strongly suggest that E. stevenii was one of the ancestors of E. boöphthona. However, E. boöphthona showed a large number of fixed alleles that were not detected in any other Australian Eremophyton species. The most likely hypothesis for the origin of E. boöphthona is that it was formed by hybridization and chromosomal doubling between an extinct diploid species and the hexaploid E. stevenii.

• 한국작물학회:학술대회논문집
• /
• 한국작물학회 1998년도 춘계 학술대회지
• /
• pp.39-40
• /
• 1998

• 대한본초학회지
• /
• 제34권5호
• /
• pp.1-12
• /
• 2019

Objectives : The consumption of licorice is large in Korea, but domestic production is insufficient due to the lack of adaptability. This study aimed to provide a morphological basis for adding interspecific hybrid licorice with improved adaptability to pharmacopoeia. Methods : This study was to establish identification criteria for the original plants, external and internal morphology of the authentic herbal medicines (Glycyrrhiza uralensis, G. glabra and G. inflata), market products and artificially interspecific hybrid forms of licorice. For this purpose, previous studies were investigated and visual and histological observations were carried out. We focused on the internal morphology by microscopic observation for securing objectivity. Finally, we proposed the identification keys for precise classification of each part. Results : 1) Original plant : Licorice species in the compendium were distinguished by the number of leaflets, presence of hair on the fruit, curvature and swelling of the fruit. 2) External morphology : Licorice species were distinguished by degree of powderiness, tearing gap, radial structure in the cross section and existence of protrusion of outer epidermis. 3) Internal morphology : Licorice species were distinguished by the degree of development of phloem fiber bundle, development of obliterated sieve, whether the secondary medullary ray are branched. In the case of interspecific hybrids, the characteristics of both species used for hybridization were mixed in all observation methods. Conclusions : These results suggest that the interspecific crossbred licorice is suitable for the pharmacopoeial standard. Therefore, it can be applied as a herbal medicine through additional supplementary study.

• 한국해양생명과학회지
• /
• 제2권2호
• /
• pp.83-89
• /
• 2017

In abalones, interspecific hybridization has been suggested as a possible means to increase production and desired traits for the industry. In Korea, Haliotis gigantea is considered a species with a larger size and higher temperature tolerance than H. discus hannai. However, H. discus hannai is considered the most valuable and popular fishery resource due to its better acceptance and higher market prices. Thus, viable interspecific hybrids have been produced by artificial inseminating H. gigantea eggs with H. discus hannai sperm. However, the reciprocal hybrid cross was not successful. In this study, the hybridity and the growth and thermal tolerance performance of the interspecific hybrids were examined. A combination of various assays revealed maximum growth occurrence at 21℃ and the higher growth rate in the hybrids than that of H. discus hannai parent. In addition, the growth and survival at high-temperature (28℃) of the hybrids was equivalent to that of the highly tolerant H. gigantea parent, suggesting new possibilities to overcome the mass mortality in H. discus hannai during high temperature periods of summer season in Korea. Furthermore, the induced interspecific hybrid status was confirmed by the presence of species-specific bands for each parental species of the random amplified polymorphic DNA (RAPD) profiles using universal rice primer (URP), which could be used as speciesspecific markers to distinguish the hybrids and their parental species.

• 한국미생물·생명공학회지
• /
• 제4권4호
• /
• pp.166-178
• /
• 1976

서로 다른 11주의 Bacillus coagulans와 13종의 Bacillus 속 14주를 deoxyribonucleic acid(DNA)-DNA hybridization method에 의해서 분류학적인 연구를 하였다. 사용한 B. coagulans 11주중 6주는 흙에서(일본 오사까교외) 분리했고, 나머지 5주는 ATCC, IFO에서 authentic strains을 얻어서 사용했다. 사용된 B. coagulans는 Bergey's Manual(8th ed)에 의거 Gordon씨들의 방법으로 동정한 결과 B. coagulans로서 확인되었다. 이렇게 동정된 B. coagulans을 분자 생물학적 차원에서 지금까지의 Conventional taxonomic study와의 관계를 연구하기 위해서 사용한 11주의 B. coagulans중 ATCC 7070을 $^3$H labeled input 즉 standard로 해서 사용했을 때 B. coagulans 내의 intraspecific DNA homology indexes는 76% 이상으로 나타났다. 이와같은 발견은 Bergey's Manual에 의거한 conventional taxonomic study의 결과와 잘 일치하고 있었음으로 새로 분리한 6주와 authentic sources로부터 받은 5주는 같은 group의 B. coagulans라는 사실을 입증해 주었다. 그리고 B. coagulans와 다른 species의 Bacillus 속 즉 B. pumilus(168), B. licheniformis (IFO 12107), B. pumilus(IFO12110), B. firmus(ATCC 14575), B. lentus(ATCC 10840), B. circulans(ATCC 4513), B.macelans(ATCC 8244), B.polymyxa, (ATCC 842), B.sphaericus(ATCC 14577), B.brevis(ATCC 8246, IFO 12334), B.laterosporus(ATCC 64), B. pantothenticus(ATCC 14576) interspecific DNA homology indexes가 각각 2～4%을 보임으로써 B. coagulans는 molecular level면에서 이들 Bacillus 속과는 상동성 관계가 적음을 나타내었다. 반면에 B. coagulans(ATCC 7050)와 E. coli(F-12)와의 상동성은 1%이하였다.

• 한국육종학회지
• /
• 제41권4호
• /
• pp.468-473
• /
• 2009

본 연구에서는 양파와 파간 종간교잡 F1의 불임성의 한 요인으로 여겨지는 염색체 이상과 교잡식물체의 염색체의 조성을 밝혀 향후 종간교잡을 이용한 양파의 형질개량과정에 종간교잡 육종의 효율성을 높이고자 하였다. 종간교잡식물체의 감수분열 염색체를 관찰한 결과 중기에 7쌍의 2가염색체와 2개의 1가염색체가 관찰되었다. 체세포 염색체수는 16개로 양파, 파의 염색체 수와 같았으며 GISH한 결과 파와 양파의 염색체가 각각 8개씩 확인되었다. 5S, 45S, TSD DNA를 probe로 하여 양파, 파, 종간잡종의 염색체의 signal을 관찰한 결과 5S와 45S rDNA는 는 3종 모두에서 1쌍의 염색체에서 나타났는데 5S의 경우 염색체의 subcentromeric 에서 45S는 부수체 염색체에서 관찰되었다. TSD DNA는 염색체의 telomeric 부위에서 관찰되었으나 양파의 경우 2쌍은 염색체의 말단부와 중심체 부분에서 signal이 관찰되었고 종간교잡 세포에서도 2쌍이 관찰되어 양파에서 유래된 염색체임이 확인되었다.

• 한국어류학회지
• /
• 제26권1호
• /
• pp.70-73
• /
• 2014

Interspecific hybrids between tiger grouper Epinephelus fuscoguttatus and giant grouper E. lanceolatus were genetically identified based on the partial sequence analysis of mitochondrial cytochrome c oxidase I (COX I) gene and nuclear recombination activating gene 2 (RAG 2) gene. Out of 585 base positions of RAG 2, a total of five nucleotide substitutions were detected between the two parental species (E. fuscoguttatus and E. lanceolatus). The hybrids had two distinct types of RAG 2 sequences corresponding to those of both parental species. Mitochondrial COX I gene sequencing showed that hybrids had sequences identical to E. fuscoguttatus. Molecular data clearly demonstrate that hybridization does occur between E. fuscoguttatus and E. lanceolatus, but with E. fuscoguttatus as the maternal parent.

• 원예과학기술지
• /
• 제33권3호
• /
• pp.453-458
• /
• 2015

A new Hibiscus cultivar 'Daewangchun', having vigorous growth, uniform plant habit, upright, compact branches, and a long red eye was developed through interspecific crosses between H. syriacus 'Samchully' (♀) and H. sinosyriacus 'Seobong' (♂). The Hibiscus breeding program was initiated in 2005 and Hibiscus ${\times}$ 'Daewangchun' was preliminarily selected as '05-R-204' in 2 008 due to its vigorous growth and stable flower quality w ith rich pink flowers. The s elected line was further evaluated for different growth characteristics, leaf shape, leaf size, and flowering characteristics, as well as tested for distinctness, homogeneity, and stability during 2010-2012. The new cultivar 'Daewangchun' had violet pink flowers (RHS N80C) with a long red eye spot, medium size and fan petals. The size of flower was 12.0 cm and size of the red eye was 3.0 cm. Leaves were 8.7 cm long and 4.7 cm wide. After plant characteristic evaluation for 3 years (2010-2012), 05-R-204 was registered as cultivar 'Daewangchun' (4731, No. of plant variety protection rights) in 2013. This newly developed cultivar 'Daewangchun' has tall vigorous growth and unique flowers with a long red eye and can be used as specimen plants in landscaping.

• Plant Biotechnology Reports
• /
• 제2권4호
• /
• pp.233-238
• /
• 2008

Kadainou R-1, an interspecific hybrid grape derived from red (Vitis ficifolia var. ganebu) and white (V. vinifera cv. Muscat of Alexandria) grapes, accumulates high concentrations of anthocyanin in the berry skin. Hence, the expression of uridine 50 -diphosphate (UDP)-glucose:flavonoid 3-O-glucosyltransferase (UFGT), the key enzyme of the anthocyanin pathway, was examined in the berry skin of Kadainou R-1. As information on gene sequences of V. ficifolia var. ganebu and other wild grape species was unavailable, we performed GeneChip hybridization using biotin-labeled genomic deoxyribonucleic acid (DNA) to investigate how the genomic sequences of V. vinifera varieties and that of V. ficifolia var. ganebu differ. The study showed a lower correlation coefficient between V. vinifera cultivars and V. ficifolia var. ganebu than that among V. vinifera cultivars. The sequences of the UFGT gene derived from both parents of the red and white cultivars were sequenced in Kadainou R1 and revealed that both were expressed irrespective of the fact that it was not expressed in the white grape (male parent).

• 한국동물학회지
• /
• 제24권4호
• /
• pp.201-215
• /
• 1981

척추동물 6종의 젖산탈수소효소의 초자내 종간 및 종내 분자교잡을 실시한 결과 드렁허리 $H_4$ 동위효소와 가물치 $M_4$ 동위효소의 교잡 및 가물치 $M_4$동위효소와 메기 동위효소의 교잡에서 $H_2M_2$ 동위효소라고 생각되는 교잡형 동위효소를 얻었다. 하등 척추동물 젖산탈수소효소의 하부단위체 M과 H의 상호간 조합에 관한 한 3차 구조의 하부단위체는 동일한 구조를 가진 하부단위체와 이량체를 형성하고 이러한 동질이량체가 사량체를 형성한다고 사료된다.