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Expression of Neurotrophic Factors and Their Receptors in Rat Posterior Taste Bud Cells

  • Park, Dong-Il;Chung, Ki-Myung;Cho, Young-Kyung;Kim, Kyung-Nyun
    • International Journal of Oral Biology
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    • v.39 no.2
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    • pp.107-114
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    • 2014
  • Taste is an important sense in survival and growth of animals. The growth and maintenance of taste buds, the receptor organs of taste sense, are under the regulation of various neurotrophic factors. But the distribution aspect of neurotrophic factors and their receptors in distinct taste cell types are not clearly known. The present research was designed to characterize mRNA expression pattern of neurotrophic factors and their receptors in distinct type of taste cells. In male 45-60 day-old Sprague-Dawley rats, epithelial tissues with and without circumvallate and folliate papillaes were dissected and homogenized, and mRNA expressions for neurotrophic factors and their receptors were determined by RT-PCR. The mRNA expressions of brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT3), receptor tyrosine kinase B (TrkB), exclusion of nerve growth factor (NGF), neurotrophin-4/5 (NT4/5), receptor tyrosine kinase A (TrkA), receptor tyrosine kinase C (TrkC), and p75NGFR were observed in some population of taste cell. In support of this result and to characterize which types of taste cells express NT3, BDNF, or TrkB, we examined mRNA expressions of NT3, BDNF, or TrkB in the $PLC{\beta}2$ (a marker of Type II cell)-and/or SNAP25 (a marker of Type III cell)-positive taste cells by a single taste cell RT-PCR and found that the ratio of positively stained cell numbers were 17.4, 6.5, 84.1, 70.3, and 1.4 % for $PLC{\beta}2$, SNAP25, NT3, BDNF, and TrkB, respectively. In addition, all of $PLC{\beta}2$-and SNAP25-positive taste cells expressed NT3 mRNA, except for one taste bud cell. The ratios of NT3 mRNA expressions were 100% and 91.7% in the SNAP25-and $PLC{\beta}2$-positive taste cells, respectively. However, two TrkB-positive taste cells co-expressed neither $PLC{\beta}2$ nor SNAP 25. The results suggest that the most of type II or type III cells express BDNF and NT3 mRNA, but the expression is shown to be less in type I taste cells.

A Study on the Value Factors of Culture Consumers for Corporate Culture Marketing through Big Data Techniques (빅데이터 기법을 통한 기업 문화마케팅을 위한 문화소비자의 가치 요소 연구)

  • Oh, Se Jong
    • The Journal of the Convergence on Culture Technology
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    • v.6 no.1
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    • pp.31-36
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    • 2020
  • Corporate Culture Marketing is a marketing tool that enhances a company's cultural image or conveys its image through culture. Culture Consumer value analysis is important predictive data in identifying the value and pursuit of life in individual consumption behavior, explaining the choice behavior of culture consumers, and serves as the basis for decision making. The research method was linked to the text mining and opinion mining techniques of big data, and extracted positive, negative and neutral words. The analysis targets culture consumers participating in concerts at Hyundai Card's 'Super Concert', which is subject to domestic consumers, and CJ ENM's 'KCON', which is subject to foreign consumers. The culture consumer value elements of corporate culture marketing are the basic conditions, and they were derived as 'Consensus Communication (Expression of Sensibility)', 'Participation Sharing(VIP Belonging)', 'Social Change Issue', 'Differentiating Services', 'Price Discount Benefit' and 'Location Quality'. In the future, we will need to foster 'Culture Technology Marketers' and apply them in areas such as arts management planning, cultural investment, cultural distribution, cultural space, Corporate Culture, CSR and K-pop marketing to enhance corporate interests and brand value and enhance brand value.

Theoretical Study on Structural Properties of Phenthylamine Derivatives (페네틸아민 유도체의 구조적 특성에 관한 이론적 연구)

  • Lee, Chul Jae
    • The Journal of the Convergence on Culture Technology
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    • v.6 no.4
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    • pp.761-766
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    • 2020
  • Phenylamine derivatives are substances that have a biochemical action and are widely applied as psychotropic drugs. In particular, with regard to the quantitative analysis of substances such as ephedrine, amphetamine, pentermine, and dopamine, many previous studies such as electrochemical, vacuum ultraviolet method, and gas chromatography have been conducted. However, there have not been many studies on the structural characteristics of molecular units. Therefore, In this study, we used (HyperChem8.0's, HC) semi-empirical PM3 method to calculate the total energy, band gap, electrostatic potential, and net charge of ephedrine, amphetamine, pentamine and dopamine to investigate the chemical properties of each derivative according to the molecular structure change. The results showed that for total energy -43,171.8, -32,9538.3, -36,407.3 and -43,061.2 Kcal/mol, respectively, while for band gaps, 10.16379377, 9.9531666, 9.7878002 and 9.0589282 eV. Also, for electrostatic potentials, 1.301~-0.045, 1.694~0.299, 0.694~-0.158 and 1.587~-0.048 respectively. Finally, looking at the distribution of net charges, the oxygen atoms, nitrogen atoms and carbon atoms were -0.312~-0.242, -0.161~-0.051 and +0.13~-0.12 respectively. These results are expected to lead to chemical action centered on phenyl radicals and oxygen and nitrogen atoms common to phenethylamine derivatives.

Evaluation of Citizens' Perception on the Utilization of Children's Parks for the Elderly (고령층을 위한 어린이공원의 활용방안에 대한 시민의식평가)

  • Lee, Kyong-bok;Hong, Kwang-Pyo;LEE, Hyukjae
    • The Journal of the Convergence on Culture Technology
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    • v.6 no.4
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    • pp.507-515
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    • 2020
  • The children's park, which was created uniformly during the industrialization period, is less utilized and is mainly used by the elderly during the day. In addition, sports facilities and rest facilities for the elderly are being installed, damaging the essence of children's parks. The main purpose of this study was to present the creation of a children's park that was appropriate to its essence and the utilization plan for the elderly. In this study, we conduct a survey to identify citizens' perception of children's parks and found that the opinions of children and the elderly were generally different. Children want to change and enjoy various games, while older people want to enjoy various programs while maintaining the status quo. As above, children do not dislike using the park with the elderly, but hope to create a separate park if possible. Meanwhile, the elderly want to renovate the current children's park and continue to use it. Therefore, in the future, some areas of children's parks will be created for the elderly or the entire area will be changed into small parks for the elderly, considering the location characteristics and population distribution of children's parks. In addition, the government should prepare measures for the introduction of programs for the elderly or maintenance using the elderly.

Korean Broadcasting Laws under the WTO Service Negotiation (WTO 서비스 협상과 국내 방송규제: 정책적 대응 및 규제정비의 필요성)

  • Song, Kyoung-Hee
    • Korean journal of communication and information
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    • v.22
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    • pp.77-106
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    • 2003
  • As globalization of communication is going on and as the media have become increasingly central to the world economy, media policy matters have become the province of world economic organizations like the IMF and the WTO. The WTO service negotiation is focused primarily on the discriminatory and quantitative barriers associated with the trade of audiovisual services. Domestic measures such as subsidization, content regulation including quotas, and licensing requirements and restrictions on foreign ownership and control are at issues here. These measures have been successfully implemented by countries wishing to withstand competition from the American audiovisual industry. The debate about trade in audiovisual services is permeated by the unstated assumption that these programs are pure commodities whose production, distribution, exhibition and in turn, values are solely determined by the market forces. It is therefore presumed that liberalization of trade in audiovisual services will benefit all, serving cultural pluralism and diversity as well as economic efficiency. However, this assumption is not shared by developing countries, the recipients of U.S. television material. They argue audiovisual sector requires a social and cultural approach, since it plays a key role in the preservation of people's identity and social bonds. They claim that it is the each state's right to define its media policy and to implement it through the means it considers fit. These clashing views over the nature of the audiovisual material and the ways in which protect cultural pluralism and diversity do not confine to be the realm of theoretical debate. Each state's interest and motivation to protect its local industry and to have a competitive advantage in the international market is working in this battle. Consolidation with the countries like Australia, Canada, and EU nations, in favour of cultural exemption, seems to be the best policy for us. However, we are not entirely free from the WTO pressures, considering relation to the U. S. This study analyzes Korean Broadcasting Law compared with those of other OECD countries and tries to propose some strategical guidelines facing WTO service negotiation in the area of broadcasting.

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Amino Acid Properties and Sensory Characteristics of Chicken Stock by Different Salt Contents (소금 첨가량을 달리한 닭 육수의 아미노산 조성 및 관능적 특성)

  • Kim, Doog-Seok;Kim, Jog-Seck;Seoung, Tae-Jong
    • Culinary science and hospitality research
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    • v.16 no.4
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    • pp.274-285
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    • 2010
  • This study attempts to develop a mass production product standardized by the application of high pressure extraction cooking(HPEC) in order to suggest a desirable direction for the development of salt contained standardized chicken stock. In our experiment on chicken stock with varying its salt content, the total free amino acid content was highest in S3, which contained 0.3% of salt In addition, when the total content of free amino acid was divided into the contents of essential amino acid, palatable amino acid, and other types of amino acid, they showed the same distribution as the total content of free amino acid. In addition, the total content of palatable amino acid was highest among the specimens. In the results of investigating the palatability of chicken stock according to salt content, saltiness increased with the increase of the salt content, but no significant difference was observed in preference for saltiness. It is believed to have come from the difference in the sensory evaluators' preference for saltiness, and it shows that the salt content has an effect on sweet taste, delicate taste, fishy smell, and color. In particular, specimen 83, which contained 0.3% of salt, showed the highest content of palatable amino acid, and the highest level of delicate taste in sensory tests, suggesting the correlation between palatable amino acid and delicate taste.

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The Effects of Franchisor's Promotion Strategies on Food Service Franchisee Trust and Business Performance (외식 프랜차이즈 가맹본부의 프로모션 활동이 가맹점의 신뢰와 경영성과에 미치는 영향에 관한 연구)

  • An, Sang-Joon;Kim, Tae-Hwan;Chang, Jun-Suk
    • Journal of Convergence for Information Technology
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    • v.7 no.6
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    • pp.259-265
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    • 2017
  • This study reviewed the existing studies in order to set up a strategy that can achieve the trust and business performance of the franchisers, who are actually operating franchised business, breaking from the research trend centered around franchise headquarters and added the factors of the franchise headquarters' strategy for expanding franchises, including word-of-mouth communication and advertising & publicity and added the exercise of recruitment agency outsourcing, flagship store, recently utilized often as preceding variables to verify the causal relation between the trust of the franchiser and the business performance of the franchise. The implications of this study, it can be concluded that the government authority for the attraction of healthy franchises can continuously attract franchises and enter the market can continuously attract franchises by increasing the trust of the franchises, and powerfully investing and managing them for the effort through word-of-mouth marketing. It is expected that this study will be helpful in the establishment of a business strategy for forming a continuous relationship as well as the franchise head office's strategy for the recruitment of new franchises.

Vegetation and flora of Hibiscus hamabo inhabited naturally in Soan Island

  • Ahn, Young-Hee;Chung, Kyu-Hwan;Park, Hee-Seung
    • Journal of Environmental Science International
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    • v.12 no.11
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    • pp.1181-1187
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    • 2003
  • Hibiscus hamabo, called "Hwang-geun", growing about 3m in height is a deciduous shrub or subtree of Malvaceae. Because the number of these species is very limited in the world, the Ministry of Environment has designated H. hamabo as a preserved plant. The Korea Forest Service also protects it strictly by law since H. hamabo is an out-of-the-way plant and possibly may be exterminated soon in Korea. Investigation for distribution and ecological characteristics of the habitat for H. hamabo was carried out on Soan Island. Two wild H. hamabo were found at the forest edge (equation omitted) along the sea coast located in the southern part of Soan Island and this was the first report in the Korean academic world. These two wild H. hamabos were growing in a naturally inhibited area. The diameters at the base were 12cm and 15cm. The Tree heights were 150cm and 210cm and the number of branches of each wild H. hamabo was 4 and 7. However, the present condition of these plants was not good. Environmental conditions of the naturally inhibited area of H. hamabo were very mild because it is located at the edge of the forest and is always sunny during the daytime since the slope of the inhibited area is facing South. The ground drained very well since the soil was made of gravels and sand. Because the percent of vegetation of the subtree layer where H. hamabo was growing was 40%, the cover degree and sociability of flex crenata trees and Eurya japonica were found to be high. In the naturally inhibited area of H. hamabo, a dominant value of Rubus parvifolius in the lower part of the herb layer was very high and many plants in Compositae, such as Artemisia princeps var. orientalis and Erigeron annuus, were also present. A dominant value of liana, such as Vitis thunbergii var. sinuata, Rosa multiflora, Clematis terniflora and Hedera rhombea, and Gramineae plants that rhizomes were well developed and aggressively propagated, such as Miscanthus sinensis var. purpurascens, Phragmites communis, Spodiopogon cotulifer and Oplismenus undulatifolius which were surveyed as high, too. These results imply that H. hamabo might be exterminated soon through a natural selection if the proper management of the naturally inhibited area of H. hamabo is not conducted continually.

A Compensation Method of Timing Signals for Communications Networks Synchronization by using Loran Signals (Loran 신호 이용 통신망 동기를 위한 타이밍 신호 보상 방안)

  • Lee, Young-Kyu;Lee, Chang-Bok;Yang, Sung-Hoon;Lee, Jong-Gu;Kong, Hyun-Dong
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.34 no.11A
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    • pp.882-890
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    • 2009
  • In this paper, we describe a compensation method that can be used for the situation where Loran receivers lose their phase lock to the received Loran signals when Loran signals are employed for the synchronization of national infrastructures such as telecommunication networks, electric power distribution and so on. In losing the phase lock to the received signals in a Loran receiver, the inner oscillator of the receiver starts free-running and the performance of the timing synchronization signals which are locked to the oscillator's phase is very severly degraded, so the timing accuracy under 1 us for a Primary Reference Clock (PRC) required in the International Telecommunications Union (ITU) G.811 standard can not be satisfied in the situation. Therefore, in this paper, we propose a method which can compensate the phase jump by using a compensation algorithm when a Loran receiver loses its phase lock and the performance evaluation of the proposed algorithm is achieved by the Maximum Time Interval Error (MTIE) of the measured data. From the performance evaluation results, it is observed that the requirement under 1 us for a PRC can be easily achieved by using the proposed algorithm showing about 0.6 us with under 30 minutes mean interval of smoothing with 1 hour period when the loss of phase lock occurs.

Estimation of Theoretical and Technical Potentials of Geothermal Power Generation using Enhanced Geothermal System (우리나라 EGS 지열발전의 이론적 및 기술적 잠재량 평가)

  • Song, Yoon-Ho;Baek, Seung-Gyun;Kim, Hyoung-Chan;Lee, Tae-Jong
    • Economic and Environmental Geology
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    • v.44 no.6
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    • pp.513-523
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    • 2011
  • We estimated geothermal power generation potential in Korea through Enhanced Geothermal System (EGS) technology following the recently proposed protocol which was endorsed by international organizations. Input thermal and physical data for estimation are density, specific heat and thermal conductivity measurements from 1,516 outcrop samples, 180 heat production, 352 heat flow, and 52 mean surface temperature data. Inland area was digitized into 34,742 grids of $1'{\times}1'$ size and temperature distribution and available heat were calculated for 1 km depth interval from 3 km down to 10 km. Thus estimated theoretical potential reached 6,975 GW which is 92 times total generation capacity of Korea in 2010. Technical potential down to 6.5 km and considering land accessibility, thermal recovery ratio of 0.14 and temperature drawdown factor of $10^{\circ}C$ was 19.6 GW. If we disregard temperature drawdown factor, which can be considered in estimating economic potential, the technical potential increases up to 56 GW.