• Molecules and Cells
• /
• 제45권12호
• /
• pp.896-910
• /
• 2022

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a highly transmissible and potentially fatal virus. So far, most comprehensive analyses encompassing clinical and transcriptional manifestation have concentrated on the lungs. Here, we confirmed evident signs of viral infection in the lungs and spleen of SARS-CoV-2-infected K18-hACE2 mice, which replicate the phenotype and infection symptoms in hospitalized humans. Seven days post viral detection in organs, infected mice showed decreased vital signs, leading to death. Bronchopneumonia due to infiltration of leukocytes in the lungs and reduction in the spleen lymphocyte region were observed. Transcriptome profiling implicated the meticulous regulation of distress and recovery from cytokine-mediated immunity by distinct immune cell types in a time-dependent manner. In lungs, the chemokine-driven response to viral invasion was highly elevated at 2 days post infection (dpi). In late infection, diseased lungs, post the innate immune process, showed recovery signs. The spleen established an even more immediate line of defense than the lungs, and the cytokine expression profile dropped at 7 dpi. At 5 dpi, spleen samples diverged into two distinct groups with different transcriptome profile and pathophysiology. Inhibition of consecutive host cell viral entry and massive immunoglobulin production and proteolysis inhibition seemed that one group endeavored to survive, while the other group struggled with developmental regeneration against consistent viral intrusion through the replication cycle. Our results may contribute to improved understanding of the longitudinal response to viral infection and development of potential therapeutics for hospitalized patients affected by SARS-CoV-2.

• Journal of Plant Biotechnology
• /
• 제37권1호
• /
• pp.25-46
• /
• 2010

최근 원유 및 석탄 등의 가격 급등에 따른 에너지 비용이 가중되고 있으며 지속적인 상승이 예상되어 기업은 물론 개인들도 에너지 사용에 보다 많은 부담을 가지게 될 것이다. 따라서 에너지원을 다양화하고 에너지를 안정적으로 공급하는 체계 구축이 시급하며, 기후변화협약의 규제에 대응하고 기존 에너지원과의 가격경쟁력을 확보하여 차세대 산업으로 성장시키기 위한 기술 확보가 절실한 시점이다. 유럽의 경우 바이오디젤 원료 및 공업적 원료로서 식물성 유지확보에 많은 정책적 배려를 하고 있다. 식용 식물성유지의 과잉생산에 따른 폭락과 미국과의 무역마찰을 피하기 위해 농업의 재편을 시도하고 있으며, BHA (Blair House Agreement)를 미국과 맺고 우루과이라운드에 입각한 국산 보조프로그램에 관련된 문제를 해결하였다. 온대지방에서 바이오디젤 연료의 원료로 적합한 유채 기름은 이미 유럽에서 상용화가 이루어지고 있으며, 유채기름을 구성하고 있는 지방산 중에서 oleic acid 함량이 높아야 겨울철 저온유동점이 영하로 낮아져 바이오디젤을 자동차연료로 쓰기에 유리하다. 중부 이남의 농지에 2모작으로 유채재배가 국내에서 가능할 경우 바이오디젤 원료확보와 동시에 헥타르 (ha) 당 7톤 이상의 이산화탄소 ($CO_2$)저감을 주는 효과가 있으며, 농업분야에서 환경 친화적 바이오에너지 생산이 가능해 지고 농민들의 소득증대와 관상용으로 지역관광 발전에도 크게 도움이 될 수 있을 것으로 생각된다.

• International Journal of Industrial Entomology and Biomaterials
• /
• 제22권2호
• /
• pp.83-93
• /
• 2011

Biochemical and enzymatic characterization for extracellular protease isolated from Cordyceps militaris cultivated on rice bran medium was investigated. C militaris produced proteolytic enzymes from 10 days after inoculation, maximum enzyme production was found at 25 days. The optimum temperature and pH of proteases production was at $25^{\circ}C$ and pH 7.0, respectively. The protease activity was observed in the four peaks (Pro-I, Pro-II, Pro-III, and Pro-IV) separated through Sephadex G-100 column chromatography. The separated protease was optimally active at $25^{\circ}C$. Optimum pH of the protease was between 7 and 8. Enzyme was also stable over at $30-80^{\circ}C$. The enzyme was highly stable in a pH range of 4-9. Protease activity was found to be slightly decreased by the addition of $Mg^{2+}$, $Mn^{2+}$, $Zn^{2+}$, $Fe^{2+}$ and $Cu^{2+}$, whereas inhibited by the addition of $Ca^{2+}$ and $Co^{2+}$ Protease activity was inhibited by protease inhibitor PMSF. On the other hand, the partially purified protease was investigated on proteolytic protease activity by zymogram gel electrophoresis using three substances (casein, gelatin and fibrin). Four active bands (F-I, FII, F-III, and F-IV) of fibrin degradation were revealed on fibrin zymogram gels. Both of F-II and FIII showed caseinolytic, fibrinolytic and gelatinolytic activities in three gels. Thermostability, pH stability, and pH-thermostability of the enzyme determined the residual fibrinolytic activity also displayed on fibrin zymogram gel. The only one enzyme (F-II) displayed over a broad range of temperature at $30-90^{\circ}C$. The FII displayed fibrinolytic activity in the pH range 3-5, but was inactivated in the range of pH 6-11. The F-I and F-III showed enzyme activity in the pH range of 6-11. In the pH-thermostability, the F-II only kept fibrinolytic activity after heating at $100^{\circ}C$ for 10, 20 and 30 min at pH 3 and pH 7, respectively. On the other hand, the F-II was retained activity until heating for 10 min under pH 11 condition. By using fibrin zymogram gel electrophoresis, extracellular fibrinolytic enzyme F-II from C. militaris showed unusual thermostable under acid and neutral conditions.

• 한국환경과학회지
• /
• 제25권4호
• /
• pp.579-588
• /
• 2016

본 연구에서는 홍어껍질에서 추출한 콜라겐 펩타이드 이화학적 특성과 비만 유발 쥐에 대한 투여 실험을 통해서 지방조직의 지질 감소효과를 조사하였으며, 홍어 껍질 콜라겐 펩타이드를 원료로 한 응용 제품 개발을 통하여 산업적 적용을 검토하였다. 일반성분 분석 결과 홍어 껍질 콜라겐 펩타이드는 대부분이 단백질로 이루어져 있는 고단백 저지방 물질이며, 분자량 측정 결과 약 1000 Da.의 저분자량이고, 아미노산 분석에서는 전형적인 콜라겐 단백질의 특성을 나타내었다. 비만 유발 쥐에 홍어 껍질 콜라겐 펩타이드를 500 mg/kg씩 18일간 경구 투여한 실험 결과에서 사료 섭취량과 음수량이 상당량 줄어 들었으며, 지방조직의 중성지방과 총 콜레스테롤 수치가 유의적으로 감소하였다. 홍어 껍질 콜라겐 펩타이드를 대량생산을 한 결과 제품의 최종 수율은 원료대비 약 17.23%로 상당히 높은 수율을 나타내었다. 이를 원료로 한 기능성 화장품 및 생활용품을 생산 및 출시하였다. 따라서 홍어 가공 부산물인 껍질을 재이용한 기능성 단백질 소재 개발을 통하여 건강기능식품과 화장품 분야에서의 기능성과 가격경쟁력을 갖춘 소재 개발에 기여 할 것으로 기대된다.

• 한국산림과학회지
• /
• 제100권4호
• /
• pp.630-638
• /
• 2011

본 연구의 목적은 북한지역의 REDD 탄소배출권 잠재량과 그 사업비용을 추정하여 REDD 사업의 경제적 타당성을 가늠해 보는 데에 있다. 북한지역의 REDD 잠재량은 국제통계의 1990년부터 2010년까지의 산림면적과 인구수를 활용하여 산정하였으며, REDD 사업비용은 북한에서 단위면적 당 생산할 수 있는 식량을 남한이 지원해준다고 가정하고, 농업 부문의 토지기회비용을 활용하여 간접 추정하였다. 북한지역의 산림전용을 참조수준 대비 25% 감소시키는 시나리오를 적용했을 때의 REDD 탄소배출권 잠재량은 20년간 4,232만~5,290만$tCO_2eq.$으로 추정되었다. 이는 우리나라 중기 온실가스 감축 목표의 28~35%에 해당하는 양이다. 한편, 북한지역에서 산림 농지로 전용한 곳에서 농사를 지을 때 얻을 수 있는 수익과 같은 수준의 REDD 사업의 수익이 보장되는 REDD 크레딧 가격, 즉 REDD 사업의 손익분기가격은 산림의 비영속성에 대한 위험율을 20%로 가정할 경우 19.19$/$tCO_2eq.$으로 추정되었다. 이 가격은 2010년 자발적 시장에서 거래된 REDD 탄소배출권 가격인 5$/$tCO_2eq.$보다 높아 경제적 타당성이 없는 것으로 나타났다. 하지만 REDD 사업이 탄소 흡수 이외에 공편익을 제공한다는 점에서는 선행연구에서 제시한 산림소실에 의한 온실가스 배출의 사회적 한계비용인 20$/$tCO_2eq.$보다 낮다는 점에서 그 타당성을 인정할 수 있다. 북한에서의 REDD 사업의 타당성은 위험율에 의해 결정된다고 할 것이다. 따라서 북한지역 REDD 사업의 경제적 타당성을 담보하기 위해서는 북한 정부와 주민들의 사업에 대한 확고한 보장과 참여가 필요하다.

• 한국기후변화학회지
• /
• 제1권2호
• /
• pp.121-131
• /
• 2010

본 연구에서는 미국에서 개발되어 IBSNAT(International Benchmark Site Network for Agrotechnology Transfer) 사업에 의해 국제적으로 널리 보급된 DSSAT 패키지(CERES-Rice 모형)의 최근 윈도우 버전(DSSAT v4)을 국내에 도입하여, 우리나라 벼 품종과 기상요소, 그리고 국립농업과학원의 정밀농업토양자료 등을 적용하여, 출수생태형별 품종유전모수를 추정한 후, GCM 기후시나리오에 따른 벼 생육 및 생산량의 변동을 시뮬레이션 방법으로 평가하였다. 온도가 상승함에 따라 출수 기간은 단축하는 것으로 조사되었다. 온도가 $2^{\circ}C$ 상승하였을 때는 7~8일 단축되는 것으로 모의되었고, $5.2^{\circ}C$ 상승하는 UKMO 기후시나리오를 적용하면 최대 16~18일 단축되는 것으로 추정되었다. 출수 기간은 출수생태형의 구분 없이 온도 상승에 따라 비슷하게 단축되는 것으로 분석되었지만, 벼 등숙 기간의 변화는 출수생태형에 따라 다른 경향을 나타내었다. 즉, 생육 기간이 길은 중만생종의 출수 기간은 온도 상승 정도에 따라 14~18일 짧아졌는데 반하여, 생육 기간이 짧은 조생종의 경우에는 1~3일 짧아져, 단축 정도가 상대적으로 적은 것으로 조사되었다. 온도가 30년 평년 기후(1971~2000년)보다 $2^{\circ}C$와 $3^{\circ}C$ 상승하는 조건에서, 전국적인 평균 벼 수량은 평년 기후에 의해 추정된 것보다 각각 4.5%와 8.2% 감소하는 것으로 전망되었고, $CO_2$ 배증에 따라 온도 상승을 가장 높게 예측하는 UKMO 전지구 기후변화모델을 적용하여, 전국적으로 벼 수량을 모의하였을 때는 평년기후에 적용하여 추정한 수량보다 전국 평균 14.9% 감소하는 것으로 분석되었다. 이는 온난화로 인한 등숙 기간의 단축뿐 아니라 고온에서의 임실율 저하, 그리고 야간고온에 의한 호흡손실 때문인 것으로 판단된다. 따라서 지역별 정밀농업기후 추정과 이에 근거한 최적 품종의 선택, 이앙기 및 수확기 등 생육 기간의 조절 등 온난화 대응기술의 개발이 필요하다.

• Journal of Medicine and Life Science
• /
• 제17권2호
• /
• pp.47-52
• /
• 2020

Glucagon regulates glucose and fat metabolism as well as being involved in the production of ketone bodies. The new antidiabetic drug, a sodium-glucose co-transporter-2 inhibitor, increases glucagon, and reduces the risk of cardiovascular death and hospitalization due to heart failure. The presence of metabolic syndrome is an important risk factor for cardiovascular diseases(CVD) in type 2 diabetes(T2DM) patients. We, thus, investigated the association between glucagon levels and metabolic syndrome in T2DM patients. This cross-sectional study involved 317 T2DM patients. Fasting and postprandial (30 min after ingestion of a standard mixed meal) glucagon levels were measured. Metabolic syndrome was defined according to the criteria of the International Diabetes Federation. A multiple regression logistic analysis was employed for statistical evaluation. A total of 219 (69%) subjects had metabolic syndrome. The fasting and postprandial glucagon levels did not differ between the group with metabolic syndrome and the group without. Postprandial glucagon levels increased significantly with the increase in the number of metabolic syndrome components, but the fasting levels did not. However, a hierarchical logistic regression analysis revealed that the postprandial glucagon levels did not contribute significantly to metabolic syndrome even after adjusting for other covariates. Fasting and postprandial glucagon levels are not associated with metabolic syndrome in T2DM patients. However, further studies are needed to investigate the relationship between glucagon and cardiovascular risk in patients with T2DM.

• 한국환경과학회지
• /
• 제26권11호
• /
• pp.1255-1266
• /
• 2017

Phosphorus (P) is an essential and major nutrient for both plants and animals. However, anthropogenic P in the environment may cause severe problems such as the deterioration of water quality. Therefore, it is essential for the Korean government to manage P in the agricultural sector. The annual P budget for Korea was 46 kg P ha-1 in 2013, placing Korea in second among Organisation for Economic Co-operation and Development (OECD) countries. P surplus and deficiency in agricultural lands can be estimated according to the P budget, which is one of the OECD agri-environment indicators. In the P budget, it is important to ensure consistency in the input-output data sources, in order to apply national and regional policies for the environmentally sound management of agricultural P. This study examines the impacts on the input-output data sources in the regional P budget in Korea. P budgets were between 99-145 kg-P/ha, depending on different data sources. We suggest two recommended data combinations (DC 1 and DC 2) for reliability of the data. P budgets calculated using DC 1 and DC 2 were 128 kg-P/ha and 97 kg-P/ha, respectively. According to the results, one of the core factors affecting P budgets was crop production. In this study, DC 2 was recommended rather than DC 1 in order to consider the cultivated areas for various crops. It is also necessary to analyze the sensitivity of the coefficients used in P budget in the future.

• 한국환경과학회지
• /
• 제27권10호
• /
• pp.809-820
• /
• 2018

DeNOx experiments for the effects of hydrocarbon additives on diesel SNCR process were conducted under oxidizing diesel exhaust conditions. A diesel-fueled combustion system was set up to simulate the actual cylinder and head, exhaust pipe and combustion products, where the reducing agent $NH_3$ and $C_2H_6/diesel$ fuel additives were separately or simultaneously injected into the exhaust pipe, used as the SNCR flow reactor. A wide range of air/fuel ratios (A/F=20~40) were maintained, based on engine speeds where an initial NOx level was 530 ppm and the molar ratios (${\beta}=NH_3/NOx$) ranged between 1.0~2.0, together with adjusting the amounts of hydrocarbon additives. Temperature windows were normally formed in the range of 1200~1350K, which were shifted downwards by 50~100K with injecting $C_2H_6/diesel$ fuel additives. About 50~68% NOx reduction was possible with the above molar ratios (${\beta}$) at the optimum flow #1 ($T_{in}=1260K$). Injecting a small amount of $C_2H_6$ or diesel fuel (${\gamma}=hydrocarbon/NOx$) gave the promising results, particularly in the lower exhaust temperatures, by contributing to the sufficient production of active radicals ($OH/O/HO_2/H$) for NOx reduction. Unfortunately, the addition of hydrocarbons increased the concentrations of byproducts such as CO, UHC, $N_2O$ and $NO_2$, and their emission levels are discussed. Among them, Injecting diesel fuel together with the primary reductant seems to be more encouraging for practical reason and could be suggested as an alternative SNCR DeNOx strategy under diesel exhaust systems, following further optimization of chemicals used for lower emission levels of byproducts.

• 한국환경과학회지
• /
• 제17권5호
• /
• pp.537-546
• /
• 2008

The experiment was conducted to determine the effect of the mixed herbal medicine for the substitution of antibiotics on the performance of laying hens. Day old hyline 1,500 layer chicks were randomly assigned to 4 treatments. Control were 600 and three each treatment was 300. Eggs were collected at every weeks for measuring egg production and week 26, 27, 28 for chemical analyses. Chemical analyses were done for fatty acid profile of egg yolks, amino acid, antibiotics residue in eggs(collect eggs after supplying OTC 21ppm/bird/day to control for four day at 26, 27, 28) during the laying period($19{\sim}77wk$). On $0{\sim}4$ week, T-3 showed higher feed intake than those fed the other diets and on $5{\sim}13$ week, T-2 showed highest feed intake among treatments. After birds were moved ($14{\sim}18$ wk) CT-3 showed highest, but for overall rearing period, there was not significantly different among treatments. In conclusion the mixed herbal medicine did not tended to influence palatability to rearing birds. Egg quality(egg yolk color, haugh unit, eggshell breaking strength) showed no difference among treatments. Ratio of unsaturated fatty acid (mg/100g)/saturated fatty acid (mg/100g) was not difference among treatments, but linolenic acid and docosahexaenoic acid(DHA) of the mixed herbal medicine treatments showed higher then control. The antibiotics residue of CT, T treatments egg was not detected and control was not detected or below allowance. In conclusion the mixed herbal medicine can be possible to feed laying hen without antibiotics.