• Title/Summary/Keyword: International Agricultural Research Institute

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Occurrence and eradication of Plum pox virus on Ornamentals in Korea, 2016-2017 (2016-2017년 국내 핵과류에서의 자두곰보병 발생 및 방제)

  • Kim, Mikyeong;Kim, Gi-Su;Kwak, Hae-Ryun;Kim, Jeong-Eun;Seo, Jang-Kyun;Hong, Seong-Jun;Lee, Gyeong-Jae;Kim, Ju-Hui;Choi, Min-Kyeong;Kim, Byeong-Ryeon;Kim, Ji-Gwang;Han, In-Yeong;Lee, Hyeon-Ju;Won, Heon-Seop;Kang, Hyo-Jung;Han, Jong-Woo;Ko, Suk-Ju;Kim, Hyo-Jeong;Kim, Seung-Han;Lee, Jung-Hywan;Choi, Hong-Soo
    • Research in Plant Disease
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    • v.25 no.1
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    • pp.8-15
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    • 2019
  • Plum pox virus (PPV) is a significant viral disease in Prunus spp. worldwide. A nationwide survey was started in Prunus spp. orchards, since PPV was first detected from peach in Korea, 2015. During 2016-2017, samples were collected from 30,333 trees in 1,985 orchards of stone fruits in 8 provinces and 4 cities, Korea and tested by RT-PCR using specific PPV primer set. As a result, 21 trees including peach (9 trees), Japanese apricot (4 trees), plum (1 tree), apricot (7 trees) in 10 orchards were infected and controlled by eradication program. Amplicons of the expected size (547 bp) were obtained from total RNA of seven peach trees in 2016, and directly sequenced. BLAST analysis revealed the highest nucleotide (NT) identity (99%) with a PPV D isolates (LC331298, LT600782) in Genbank. The seven isolates from shared nt sequence identities of 98 to 100% with one another. Phylogenetic analysis showed the isolates in peach clustered closely with the PPV-D isolates from Korea, Japan, USA, and Canada. This is, to our knowledge, the first report of the presence of PPV in Prunus spp. orchards in Korea, 2016-2017, we hope that our results and efforts will contribute to effective measures for eradication of PPV.

Comparison of Carcass and Sensory Traits and Free Amino Acid Contents among Quality Grades in Loin and Rump of Korean Cattle Steer

  • Piao, Min Yu;Jo, Cheorun;Kim, Hyun Joo;Lee, Hyun Jung;Kim, Hyun Jin;Ko, Jong-Youl;Baik, Myunggi
    • Asian-Australasian Journal of Animal Sciences
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    • v.28 no.11
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    • pp.1629-1640
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    • 2015
  • This study was performed to compare carcass traits, sensory characteristics, physiochemical composition, and contents of nucleotides, collagen, and free amino acids among quality grades (QG) and to understand the association between QG and above parameters in loin and rump of Korean cattle steer. Loin and rump samples were obtained from 48 Korean cattle steers with each of four QG (QG 1++, 1+, 1, and 2; average 32 months of age). Carcass weight and marbling score (MS) were highest in QG 1++, whereas texture score measured by a meat grader was highest in QG 2. A correlation analysis revealed that MS (r = 0.98; p<0.01) and fat content (r = 0.73; p<0.01) had strong positive correlations with QG and that texture had a strong negative correlation (r = -0.78) with QG. Fat content in loin was highest but protein and moisture contents were lowest in QG 1++. Our results confirmed that a major determinant of QG is the MS; thus, intramuscular fat content. The International Commission on Illumination $L^*$, $a^*$, and $b^*$ values in loin were highest in QG 1++. Numeric values of shear force in loin were lowest in QG 1++, whereas those of tenderness, juiciness, and overall acceptability tended to be highest in QG 1++ without statistical significance. QG was strongly correlated with juiciness (r = 0.81; p<0.01) and overall acceptability (r = 0.87; p<0.001). All sensory characteristics were higher (p<0.05) in loin than those in rump. Adenosine-5'-monophosphate (AMP) and inosine-5'-monophosphate (IMP) contents in both loin and rump did not differ among QGs. No nucleotide (AMP, IMP, inosine, hypoxanthine) was correlated with any of the sensory traits. Total, soluble, and insoluble collagen contents in loin were higher in QG 1++ than those in QG 1. All three collagens had lower content in loin than that in rump. All three collagens were positively correlated with tenderness, juiciness, and overall acceptability. Glutamic acid content did not significantly differ among the four QGs in either loin or rump. In conclusion, it is confirmed that QG is associated with sensory traits but nucleotide contents in beef may not be a major factor determining meat palatability in the present study.

Oviposition Activity of Black Soldier fly (Hermetia illucens) under Artificial Illumination

  • Park, Kwan-Ho;Han, Moon-Hee;Lee, Seokhyun;Kim, Eun-Sun;Song, Myung-Ha;Kim, Won-Tae;Choi, Ji-Young;Kim, Hong Geun
    • International Journal of Industrial Entomology and Biomaterials
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    • v.35 no.2
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    • pp.100-105
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    • 2017
  • Under natural conditions, black soldier fly, Hermetia illucens (L.) (Diptera: Stratiomyidae), colonizes in warm temperate regions, and is active in Korea from May through October. Information on black soldier fly rearing, which is affected by seasonal factors in Korea, is limited. Oviposition by black soldier flies is dependent on light intensity and wavelength. Therefore, continuous mass rearing of this fly requires determination of optimal artificial conditions of illumination. In this study, we compared the number of eggs laid under an artificial light source (750 watt HPL lamp) versus nature sunlight. Our results showed that compared to oviposition under natural sunlight, the use of one or two lamps for 7 hours, resulted in only 43 and 76%, of the total number of eggs laid under natural sunlight, respectively. We also investigated the hatchability of oviposited eggs under artificial illumination and under natural sunlight. The hatching rate under the former was much significantly lower than under the latter. Further detailed research is required to develop methods for successful mass rearing of black soldier fly throughout the year by means of an indoor system.

Fruit Morphology, Citrulline, and Arginine Levels in Diverse Watermelon (Citrullus lanatus) Germplasm Collections

  • Awraris Derbie Assefa;On-Sook Hur;Na-Young Ro;Jae-Eun Lee;Ae-Jin Hwang;Bit-Sam Kim;Ju-hee Rhee;Jung Yoon Yi;Ji Hyun Kim;Ho-Sun Lee;Jung-Sook Sung;Myung-Kon Kim;Jae-Jong Noh
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2020.08a
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    • pp.33-33
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    • 2020
  • Watermelon (Citrullus lanatus) is a non-seasonal, economically important, cucurbit cultivated throughout the world with Asia as a continent contributing the most. As part of the effort in diversifying watermelon genetic resources in the already cultivated group, this study was devoted to providing baseline data on morphological quality traits and health-beneficial phytonutrients of watermelon germplasm collections, thereby promoting watermelon research and cultivation programs. To this end, we reported morphological traits, citrulline, and arginine levels of watermelon genetic resources obtained from the gene bank of Agrobiodiversity Center, Republic of Korea, and discussed the relationship between each other. Diverse characteristics were observed among many of the traits. But, most of the genetic resources (>90%) were either red or pink-fleshed. Korean origin fruits contained intermediate levels of soluble solid content (SSC) while The USA, Russian, Tajikistan, Turkmenistan, Taiwan, and Uruguay originated had generally the highest levels of soluble solids. The citrulline and arginine contents using HPLC method were ranged from 6.9 to 52.1 mg/g (average, 27.3 mg/g) and 1.8 to 21.3 mg/g (average, 9.8 mg/g), respectively. The citrulline content determined using Citrulline Assay Kit was ranged from 6.5 to 42.8 mg/g (average, 27.0 mg/g). Resources with high citrulline and arginine levels contained low SSC. Whereas, red- and pink-colored flesh samples had less citrulline compared to yellow and orange. In addition to the profiling of morphological characters and phytonutrients, molecular marker characterization and identification of sources of resistance to diseases and pests are recommended for a more complete diversity analysis of watermelon genetic resources.

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Cooperation Measures for Agricultural Infrastructure Development in North Korea (북한 농업생산기반조성 현황과 협력 방안)

  • Choe Jin Wook
    • Proceedings of the Korean Society of Crop Science Conference
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    • 1998.10a
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    • pp.134-158
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    • 1998
  • The objectives of this article are to identify the current status of agricultural infrastructure in North Korea and to suggest some cooperation measures among South and North Koreas and international agencies in order to develop the North Korea's agricultural infrastructure. The area of cultivated land in North Korea is 1,992 thousand ha (paddy field: 585 thousand ha: upland: 1,407 thousand ha) in 1997. Major water use facilities in North Korea are 1,900 reservoirs (included 100 big dams), 36,400 irrigation pumping stations, 1,600 drainage pumping stations, and 14'a,000 wells. In addition, there is 'two thousand Ri canal construction project' linking the rivers of Aprok, Daeryong, Daedong, Jaeryong, and Yesong. The unit of paddy land consolidation is about 1 ha which is regarded as rational for agricultural mechanization. The project of 'Darak' upland construction to create small size farmland, which has been carried out since 1976 has been unsuccessful due to the shortage of construction equipment. The area of farmland created by reclamation by 1995 is only 75 thousand ha although the potential project area is at)out 320 thousand ha along the western coast. It is due to the fact that civil engineering technologies and equipments are old and investment funds are insufficient. These are a few suggested areas of cooperation among South and North Korea and international agencies in order to improve North Korea's agricultural infrastructure : i) see land reclamation and land consolidation projects to increase paddy fields for rice production; ii) rehabilitation project in farm land areas devastated by flood; and iii) agricultural water development project which including diagnosing and rehabilitating irrigation and drainage facilities.

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Regional Application of the OECD Phosphorus Budget: Comparison of the Input-Output Data Sources (OECD 인 수지 산정법의 지역단위 적용 연구: 유출입 자료 출처 비교)

  • Lim, Do Young;Ryu, Hong-Duck;Chung, Eu Gene;Kim, Yongseok
    • Journal of Environmental Science International
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    • v.26 no.11
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    • pp.1255-1266
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    • 2017
  • Phosphorus (P) is an essential and major nutrient for both plants and animals. However, anthropogenic P in the environment may cause severe problems such as the deterioration of water quality. Therefore, it is essential for the Korean government to manage P in the agricultural sector. The annual P budget for Korea was 46 kg P ha-1 in 2013, placing Korea in second among Organisation for Economic Co-operation and Development (OECD) countries. P surplus and deficiency in agricultural lands can be estimated according to the P budget, which is one of the OECD agri-environment indicators. In the P budget, it is important to ensure consistency in the input-output data sources, in order to apply national and regional policies for the environmentally sound management of agricultural P. This study examines the impacts on the input-output data sources in the regional P budget in Korea. P budgets were between 99-145 kg-P/ha, depending on different data sources. We suggest two recommended data combinations (DC 1 and DC 2) for reliability of the data. P budgets calculated using DC 1 and DC 2 were 128 kg-P/ha and 97 kg-P/ha, respectively. According to the results, one of the core factors affecting P budgets was crop production. In this study, DC 2 was recommended rather than DC 1 in order to consider the cultivated areas for various crops. It is also necessary to analyze the sensitivity of the coefficients used in P budget in the future.

Callus induction and plant regeneration from immature zygotic embryos of various maize genotypes (Zea mays L .) (다양한 계통의 옥수수 미성숙배로부터 캘러스 유도와 식물체 재분화)

  • Hong, Joon Ki;Park, Ki Jin;Lee, Gang-Seob;Kim, Dool Yi;Kim, Ju-Kon;Lee, Seung Bum;Suh, Eun Jung;Lee, Yeon-Hee
    • Journal of Plant Biotechnology
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    • v.44 no.1
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    • pp.49-55
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    • 2017
  • We investigated the callus induction and plant regeneration ability of 16 maize genotypes, including the Korean inbred lines, using 9 to 15 day-old immature zygotic embryos from maize grown in pots and from field cultures. Immature zygotic embryos placed on MS medium supplemented with L-proline 0.7 g/L, MES 0.5 g/L, Dicamba 1.5 mg/L, 2,4-D 0.5 mg/L, $AgNO_3$ 4 mg/L, and sucrose 20 g/L, showed the highest frequency of callus induction. The highest number of shoots regenerated when the embryogenic callus were transferred to MS medium supplemented with 5 mg/L zeatin. The root formation was observed when shoots were grown on MS medium supplemented with 0.2 mg/L indole-3-butyric acid (IBA). Additionally, under the same culture conditions, immature zygotic embryos from maize grown in the field also had a high frequency of plant regeneration. Except one genotype, 15 genotypes showed callus induction and shoot regeneration. Among the 16 genotypes tested, H99, B98, HW3, and B73 yielded the best plant regeneration. H99 showed maximum shoot formation from the primary embryogenic callus. The results suggest that genotypes and growth conditions of the maize plant plays very important roles for enhancing the embryogenesis competence of immature zygotic embryos. The successful regeneration from immature zygotic embryos of maize inbred lines provides a basis for molecular breeding of new cultivars by genetic transformation.

Evaluation of Flow-Pollutant Load Delivery Ratio Equations on Main Subwatersheds within Juam Lake (농촌유역 유량-유달율 단순회귀식을 이용한 주암호 상류유역의 유달율 추정가능성 평가)

  • Jung, Jae-Woon;Lim, Byung-Jin;Choi, Dong-Ho;Choi, Yu-Jin;Lee, Kyoung-Sook;Kim, Young-Joo;Kim, Kap-Soon;Chang, Nam-Ik;Yoon, Kwang-Sik
    • Journal of Environmental Science International
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    • v.21 no.10
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    • pp.1235-1244
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    • 2012
  • The objective of this study is to evaluate Flow-Pollutant load delivery ratio equations developed from rural watershed on main subwatersheds within Juam Lake. Two regression equations for BOD and three equations for T-P were evaluated on Bosung cheon, Dongbok cheon, Songgwang cheon, Naenam cheon, and Sinpyeon cheon. The results show that estimation of BOD delivery ratio using flow-delivery equation is reliable when relative composition of discharge load of pollutant sources of a watershed is similar to those of watershed where the equation developed. On the other hand, application of regression equation for T-P was feasible when the landuse pattern and relative composition of discharge load of pollutant sources of a watershed is similar to those of watershed where the equation developed.

The Performance and Productivity Changes of Agricultural Sector in Transition Countries - Focusing on the Effects of Policy Reform and Initial Conditions - (체제전환국가의 농업부문 생산성 및 생산성 변화 -제도개혁과 초기조건의 영향을 중심으로-)

  • Kim, Hanho;An, Donghwan
    • International Area Studies Review
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    • v.12 no.3
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    • pp.3-23
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    • 2008
  • We investigated the effects of policy reform, industrial transformation, and initial conditions on the agricultural productivity changes in 28 transition countries. Our findings are: (i) Asian and CEE transition countries performed better than CIS countries, while the performance improvement of CEE countries seems to be more prominent compared to that of Asian and CIS countries; (ii) The technical progress proved to be the main source of productivity growth, particularly in CEE countries; (iii) Reform policy and industrial transformation seems to have positive effects on the performance of agricultural sector and its changes; (iv) The initial conditions also matter.