• 수산해양교육연구
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• 제24권4호
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• pp.591-601
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• 2012

양식 넙치에 Edwardsiella tarda 또는 Streptococcus iniae를 단독 또는 혼합감염하여 넙치에서 병원성을 비교하였다. 넙치에 단독 또는 혼합감염 후 혈액생화학적 성상으로 alanine aminotransferase (ALT), aspatate aminotransferase (AST), glucose 농도, total protein 농도를 조사하였으며, 라이소자임 활성, 신장과 비장 조직 중의 세균수, 조직병리학적 변화와 누적폐사율을 조사하였다. 혼합감염구는 다른 시험구에 비하여 누적폐사율, 신장과 비장 조직 중의 세균수, AST활성과 병리조직학적 변화가 크게 나타났으나, 라이소자임 활성에서는 유의적인 차이가 보이지 않았다. 따라서 E. tarda와 S. iniae의 혼합감염은 신장과 비장 조직 중의 세균수 증가와 내부 장기의 손상으로 숙주에 높은 병원성을 나타낸 것으로 판단된다.

• 한국환경과학회지
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• 제28권2호
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• pp.183-189
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• 2019

Curcumin ($C_{21}H_{20}O_6$) is a hydrophobic polyphenol found in turmeric. Although curcumin has been used as a natural medicine, its major limitation is related to poor absorption from the gut. Therefore, we developed a method for preparation of Curcumin Nanospheres (CN) to improve the aqueous-phase solubility of curcumin and investigate the functional role of CN in promoting feed efficiency and odor reduction in mice. CN showed inhibitory effects on actate dehydrogenase (LDH) cytotoxicity induced by ecotoxic substance toluene in gut epithelial HCT116 cells. In addition, the weights of internal organs (liver, heart, kidneys, and spleen) and the levels of serum Glutamate Oxaloacetate Transaminase (GOT), Glutamate Pyruvate Transaminase (GPT), and LDH did not show significant differences between mice administered oral CN for two weeks and compared to the control group. Interestingly, CN not only reduced hydrogen sulfide ($H_2S$) and ammonia ($NH_3$) levels and fecal odor, but also improved feed efficiency in mice. These results demonstrate that oral nano-delivery of anti-ecotoxicological CN is a functional system to deliver curcumin to the gut to improve feed efficiency and reduce fecal odor in mice.

• 한국음향학회지
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• 제38권4호
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• pp.443-449
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• 2019

심혈관계 질환 환자가 발생 하였을 때, 환자의 뇌와 내부 장기를 보호하고 생존율을 높이기 위해서는 사건발생 이후 신속하게 응급 의료 서비스를 제공하여 경과 시간을 줄이는 것이 가장 중요하다. 뿐만 아니라 심폐소생술의 실시를 위한 판단은 경동맥의 맥을 직접 짚는 '경동맥 촉진법'은 실시자의 주관적인 판단과 약해진 심혈관 기능에 따른 뇌혈류 차단을 할 수 있다. 본 연구는 개발된 다중 초음파 도플러 채널 쌍과 산소포화도 측정 모듈이 결합된 패치형 프로브를 이용하여 경동맥의 혈류 속도, 맥박, 산소포화도를 생체 내 실험을 통해 정성적으로 측정하였다. 따라서 본 시스템은 응급 상황에서 정량적이고 신속하게 환자의 심폐 기능을 모니터링 하여 심폐소생술 판단 여부를 객관적으로 제공하여 응급 상황 시 심혈관계 질환 환자의 생존률을 높일 수 있는 차세대 진단 기기로 활용 될 수 있다.

• 셀메드
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• 제11권1호
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• pp.3.1-3.6
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• 2021

Ascites is the accumulation of fluid in the peritoneal cavity, and it is generally allied with liver disease like cirrhosis of the liver. The ascites is commonly associated with liver cirrhosis, malignancy, or cardiovascular disease today. The liver cirrhosis is the most significant cause of ascites in developed countries as per the available data. In the modern medicine system, the treatment includes the restriction of sodium intake, use of diuretics, and paracentesis done in severe cases. There are many side effects of synthetic chemical diuretics, and their long-term use is not recommended. The alternative medicine like Unani system of medicine provides natural diuretics and drugs which also have protective effects on kidney, liver and other internal organs. It is well known in all drug systems that the prognosis is poor in ascites, but the use of Unani herbal medicines can minimize the side effects caused by synthetic chemical diuretics. The natural herbs used for the management of ascites have fewer side effects as compared to synthetic drugs. The natural drugs can be used for longer duration and provide some dietary supplement which improves the quality of life. This review addressed the thorough treatment of ascites by natural diet and medications.

• Nuclear Engineering and Technology
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• 제54권12호
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• pp.4698-4707
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• 2022

As part of the ICRP Task Group 103 project, we developed ten thyroid models for the pediatric mesh-type reference computational phantoms (MRCPs). The thyroid is not only a radiosensitive target organ needed for effective dose calculation but an important source region particularly for radioactive iodines. The thyroid models for the pediatric MRCPs were constructed by converting those of the pediatric voxel-type reference computational phantoms (VRCPs) in ICRP Publication 143 to a high-quality mesh format, faithfully maintaining their original topology. At the same time, we improved several anatomical parameters of the thyroid models for the pediatric MRCPs, including the mass, overlying tissue thickness, location, and isthmus dimensions. Absorbed doses to the thyroid for the pediatric MRCPs for photon external exposures were calculated and compared with those of the pediatric VRCPs, finding that the differences between the MRCPs and VRCPs were not significant except for very low energies (<0.03 MeV). Specific absorbed fractions (target ⟵ thyroid) for photon internal exposures were also compared, where significant differences were frequently observed especially for the target organs/tissues close to the thyroid (e.g., a factor of ~1.2-~327 for the thymus as a target) due mainly to anatomical improvement of the MRCP thyroid models.

• 한국발생생물학회지:발생과생식
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• 제27권2호
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• pp.91-99
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• 2023

The sea cucumber, Apostichopus japonicus, is one of the most valuable aquatic species. The color of body wall and appearance are important for the value of sea cucumbers. To examine expression pattern of long-chain acyl-coenzyme A dehydrogenase (LCAD), nuclear distribution C-containing protein 3 (NUDCD3), and receptor tyrosine kinase Tie-1 (TIE1), previously reported as differently expressed genes during the pigmentation of sea cucumber, we analyzed the temporal profiles of LCAD, NUDCD3, and TIE1 mRNAs in LED-exposed and light-shielded A. japonicus. Real-time quantitative PCR revealed that the LCAD, NUDCD3, and TIE1 mRNAs from the juveniles at 40-60 days post-fertilization (dpf) exhibited increasing patterns as compared to those of an early developmental larva (6-dpf). At 60-dpf juveniles, the LCAD and TIE1 mRNA levels of LED-exposed individuals were higher than those of light-shielded ones, whereas at 40-dpf and 50-dpf juveniles, the NUDCD3 mRNA expression was higher in the light-shielded condition (p<0.05). In the pigmented juveniles (90-dpf), the LCAD and TIE1 mRNA levels tended to show higher levels in red individuals than those in green ones, but there was a conversely higher level of NUDCD3 mRNA in green larva. In situ examination of LCAD and NUDCD3 mRNAs in light-shielded 6-dpf larva revealed that both genes are mainly expressed in the internal organs compared to the body surface. Together, these results may provide insights into the differential gene expression of LCAD, NUDCD3, and TIE1 during pigmentation process of the sea cucumber.

• 한국어병학회지
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• 제37권1호
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• pp.79-88
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• 2024

Aeromonas spp. 감염은 관상어 산업뿐만 아니라 양식업에서도 상당한 경제적 손실을 초래하는 것으로 보고되고 있다. 한편 2022년 12월부터 2023년 1월 사이에 국내에서 사육중인 금붕어(Crassius auratus)에서 Aeromonas병증을 보이는 어체에 대해서 발병 원인균을 규명하기 위해서 조사 하였다. 병증을 보이는 금붕어의 피부와 내부 장기에서 그람 음성균이 분리되었다. Biochemical test와 16S rRNA gene으로는 Aeromonas 속까지 동정할 수 있었으나 종레벨까지는 구분하기 어려웠다. 이에 가장 상동성이 높았던 A.veronii를 대상으로 species-specific PCR을 수행했고 명확히 종 수준으로 동정할 수 있었다. 아울러서 장독소 유전자, 감염 실험 및 항생제 내성 분석을 수행하였다. 금붕어에 대한 균주의 생체 내 병원성 시험 결과, 실험 주사 후 일주일 이내에 감염된 숙주에서 100%의 치사율을 나타냈다. 장독소 유전자인 act 독력 유전자가 검출되었다. 균주의 항균제 감수성 분석 결과 대부분의 항균제에 감수성을 보였으나, Ampicillin, Imipenem, Meropenem 및 Clindamycin 약제에는 내성을 보였다.

• Journal of Radiation Protection and Research
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• 제49권1호
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• pp.50-64
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• 2024

Background: The reference dose coefficients (DCs) of the International Commission on Radiological Protection (ICRP) have been widely used to estimate organ doses of individuals for risk assessments. This approach has been well accepted because individual anatomy data are usually unavailable, although dosimetric uncertainty exists due to the anatomical difference between the reference phantoms and the individuals. We attempted to quantify the individual variation of organ doses for photon external exposures by calculating and comparing organ DCs for 30 individuals against the ICRP reference DCs. Materials and Methods: We acquired computed tomography images from 30 patients in which eight organs (brain, breasts, liver, lungs, skeleton, skin, stomach, and urinary bladder) were segmented using the ImageJ software to create voxel phantoms. The phantoms were implemented into the Monte Carlo N-Particle 6 (MCNP6) code and then irradiated by broad parallel photon beams (10 keV to 10 MeV) at four directions (antero-posterior, postero-anterior, left-lateral, right-lateral) to calculate organ DCs. Results and Discussion: There was significant variation in organ doses due to the difference in anatomy among the individuals, especially in the kilovoltage region (e.g., <100 keV). For example, the red bone marrow doses at 0.01 MeV varied from 3 to 7 orders of the magnitude depending on the irradiation geometry. In contrast, in the megavoltage region (1-10 MeV), the individual variation of the organ doses was found to be negligibly small (differences <10%). It was also interesting to observe that the organ doses of the ICRP reference phantoms showed good agreement with the mean values of the organ doses among the patients in many cases. Conclusion: The results of this study would be informative to improve insights in individual-specific dosimetry. It should be extended to further studies in terms of many different aspects (e.g., other particles such as neutrons, other exposures such as internal exposures, and a larger number of individuals/patients) in the future.

• 대한영상의학회지
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• 제85권2호
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• pp.474-479
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• 2024

Mucormycosis는 Mucorales에 의해 유발되는 진균 감염으로, 다양한 장기를 침범할 수 있으며 그중에서도 폐점균증은 드물지만 특히 면역 저하 환자에서 생명을 위협하는 기회 감염이다. 폐점균증의 영상 소견은 다양하지만, 폐점액진균증 환자에서 점점 커지는 폐동맥색전증을 모방하는 경우는 드물게 보고되었다. 저자들은 골수이형성 증후군 환자에서 종괴와 폐색전증을 모방한 혈관 침습성 점막 진균증의 증례에 대해 보고하고자 한다.

• Journal of Ginseng Research
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• 제48권5호
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• pp.511-519
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• 2024

Background: The cycle of seasonal dormancy of perennating buds is an essential adaptation of perennial plants to unfavorable winter conditions. Plant hormones are key regulators of this critical biological process, which is intricately connected with diverse internal and external factors. Recently, global warming has increased the frequency of aberrant temperature events that negatively affect the dormancy cycle of perennials. Although many studies have been conducted on the perennating organs of Panax ginseng, the molecular aspects of bud dormancy in this species remain largely unknown. Methods: In this study, the molecular physiological responses of three P. ginseng cultivars with different dormancy break phenotypes in the spring were dissected using comparative genome-wide RNA-seq and network analyses. These analyses identified a key role for abscisic acid (ABA) activity in the regulation of bud dormancy. Gene set enrichment analysis revealed that a transcriptional network comprising stress-related hormone responses made a major contribution to the maintenance of dormancy. Results: Increased expression levels of cold response and photosynthesis-related genes were associated with the transition from dormancy to active growth in perennating buds. Finally, the expression patterns of genes encoding ABA transporters, receptors (PYRs/PYLs), PROTEIN PHOSPHATASE 2Cs (PP2Cs), and DELLAs were highly correlated with different dormancy states in three P. ginseng cultivars. Conclusion: This study provides evidence that ABA and stress signaling outputs are intricately connected with a key signaling network to regulate bud dormancy under seasonal conditions in the perennial plant P. ginseng.