• 제목/요약/키워드: Intermedia

검색결과 262건 처리시간 0.025초

Optimization of Extraction Conditions for Active Compounds of Herbal Medicinal Formula, DF, by Response Surface Methodology

  • Jeong, Birang;Choi, Seong Yeon;Jang, Hyeon Seok;Yoo, Guijae;Kim, Seung Hyun;Kim, Jung-Hwan;Kwon, Yong Soo;Roh, Jong Seong;Yoon, Yoosik;Shin, Soon Shik;Yang, Heejung
    • Natural Product Sciences
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    • 제23권1호
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    • pp.9-15
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    • 2017
  • DF formula is comprised of three traditional herbs, Ephedra intermedia, Rheum palmatum and Lithospermum erythrorhizon, and locally used for treating of the metabolic diseases, such as obesity and diabetes in Korea. We tried to optimize the extraction conditions of two major components, (-)-ephedrine and (+)-pseudoephedrine, in DF formula using response surface methodology with Box-Behnken design (BBD). The experimental conditions with 70% for EtOH concentrations, 4.8 hour for extraction hours and 8.7 times for the solvent to material ratio were suggested for the optimized extraction of DF formula with the highest amounts of (-)-ephedrine and (+)-pseudoephedrine in the designed model.

현대 패션디자인의 연속 표현[serial expression]형식에 관한 연구 (A Study on the Forms of Serial Expression in Contemporary Fashion Design)

  • 권자영;금기숙
    • 복식
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    • 제57권8호
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    • pp.114-124
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    • 2007
  • Contemporary fashion design has been represented as intermedia transcended value and spatiotemporal notion and also had a tendency to concentrate on serial process that materials are transfigured through time rather than existence. These forms related to interaction with time, space and performance as well as compositive genres, hybrid culture, compound gender define as 'serial expression' in this study. The serial expression ran be characterized that system, process, series, enumeration of sequences, depiction of performance, repetition of action in fashion collections and exhibitions of designers. The concept and circumstances made by author as a creator of fashion broaden perceptions of audiences and arouse spectators to participate in the situation as needing immediate attention. The forms of fashion and Conceptual Art in serial expression are analogous and even identical situations represent in fashion collection. Therefore analysis serial forms of art derives formative features: Narrative process, Imitation and Appropriation, Virtual reality and High technology, Hybridism and Convergence. This study suggest a framework to analyze conceptual fashion that give salience to megatrend in contemporary fashion culture on artistic point of view.

Antibacterial Activity of Sophoraflavanone G Isolated from the Roots of Sophora flavescens

  • Cha, Jeong-Dan;Jeong, Mi-Ran;Jeong, Seung-Il;Lee, Kyung-Yeol
    • Journal of Microbiology and Biotechnology
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    • 제17권5호
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    • pp.858-864
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    • 2007
  • This study investigated the antibacterial activities of sophoraflavanone G from Sophora flavescens in combination with two antimicrobial agents against oral bacteria. The combined effect of sophoraflavanone G and the antimicrobial agents was evaluated using the checkerboard method to obtain a fractional inhibitory concentration(FIC) index. The sophoraflavanone G+ampicillin(AM) combination was found to have a synergistic effect against S. mutans, S. sanguinis, S. sobrinus, S. gordonii, A. actinomycetemcomitans, F nucleatum, P. intermedia, and P. gingivalis, whereas the sophoraflavanone G+gentamicin(GM) combination had a synergistic effect against S. sanguinis, S. criceti, S. anginosus, A. actinomycetemcomitans, F nucleatum, P. intermedia, and P. gingivalis. Neither combination exhibited any antagonistic interactions(FIC index>4). In particular, the MICs/MBCs for all the bacteria were reduced to one-half$\sim$one-sixteenth as a result of the drug combinations. A synergistic interaction was also confirmed by time-kill studies for nine bacteria where the checkerboard suggested synergy. Thus, a strong bactericidal effect was exerted through the drug combinations, plus in vitro data suggested that sophoraflavanone G combined with other antibiotics may be microbiologically beneficial rather than antagonistic.

A meta-analysis of microbiota implicated in peri-implantitis

  • Han-gyoul Cho;Ran-Yi Jin;Seung-Ho Ohk
    • International Journal of Oral Biology
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    • 제48권3호
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    • pp.19-31
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    • 2023
  • Peri-implantitis is a disease affecting the tissue surrounding dental implants, destroying both soft and hard tissues. A total of 2,015 studies were collected by searching items in the National Library of Medicine, including keywords, such as "peri-implantitis," "microbiota," and "microbiome." Of them, 62 studies were screened and considered eligible for analysis. Only 16 studies qualified all criteria mentioned here: "Using PCR methods for microorganism detection," "Suggesting quantified results," "Stating obvious clinical diagnosis criteria ("Bleeding on probing," "Probing pocket depth," "Suppuration," and "Radiographic bone loss")." Only 8 studies were included in the meta-analysis because the others had special issues. Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola, Aggregatibacter actinomycetemcomitans, Prevotella intermedia, and Epstein-Barr virus were the microbiological subjects of analysis. The odds ratio (OR) between the healthy implants and peri-implantitis were calculated for each microorganism to compare two groups, and the forest plots were suggested as the visual materials. P. gingivalis (1.392 < OR < 2.841), T. forsythia (1.345 < OR < 3.221), T. denticola (2.180 < OR < 5.150), A. actinomycetemcomitans (1.975 < OR < 6.456), P. intermedia (1.245 < OR < 3.612), and Epstein-Barr virus (1.995 < OR < 9.383). The species showed that their 95% confidence interval of odds ratio was higher than 1, indicating that they were detected more frequently in periimplantitis than in healthy implants. Meanwhile, other species, such as Fusobacterium nucleatum and Staphylococcus aureus, were not included in the meta-analysis because the number of studies was insufficient.

싸리류(類)의 예확회수별(刈穫回數別) 생산성(生産性)에 관(關)한 연구(硏究) (Productivity of Lespedeza Species by the Number of Cutting Times)

  • 한영창
    • 한국산림과학회지
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    • 제23권1호
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    • pp.17-27
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    • 1974
  • 1972년(年)부터 1973년(年)까지 2개년간(個年間) 싸리 조록싸리 풀싸리를 공시재료(供試材料)로 하여 예확회수별(刈穫回數別) 생육상황(生育狀況) 생산성(生産性) 사료가(飼料價) 비료가(肥料價)의 변화(變化)를 구명(究明)코저 년간(年間) 1.2.4회(回) 예확구(刈穫區)를 설정(設定) 예확시험(刈穫試驗)을 실시한 결과(結果)를 요약(要約)하면 다음과 같다. 1. 싸리 조록싸리 풀싸리 모두가 년간(年間) 4회예확(回刈穫)의 4번예(番刈)만을 제외(除外)하고는 전년도(前年度)보다 생육(生育) 및 생산량(生産量)이 증가(增加)하였다. 2. 예확회수별(刈穫回數別) 종별(種別) 생산량(生産量)은 종간예확회수간(種間刈穫回數間) 1%가 넘는 고도(高度)의 유의성(有意性)을 인정(認定)할 수가 있으며 종간(種間)에서는 싸리 풀싸리 조록싸리의 순위(順位)로 수확량(收穫量)이 많았고 년간(年間) 4회예확(回刈穫)의 4번예(番刈)는 생육(生育) 및 수확량(收穫量)의 감소(減少)를 가져왔다. 3. 싸리 조록싸리 풀싸리 모두 년간(年間) 예확회수(刈穫回數)가 증가(增加)함에 따라 생건비(生乾比)(건중량(乾重量)/생중량(生重量)) 엽경비(葉莖比)(경중량(莖重量)/엽중량(葉重量))는 감소(減少)했다. 4. 사료가중(飼料價中) 조단백질은 생육(生育)시기가 진행(進行)함에 따라 감소(減少)하는데 반해 조섬유는 증가(增加)하였으며 특(特)히 조지방(粗脂肪)은 예확회수별(刈穫回數別) 예확시기(刈穫時期)에 따라 변화(變化)가 많았다. 5. 비료가(肥料價)는 공시종(供試種) 전부(全部) 예확시기(刈穫時期)가 늦은것이 낮게 나타났다. 6. 싸리류(類)를 사비료(飼肥料)로 사용(使用)코저 할때는 사비료가(飼肥料價)가 높은 싸리 풀싸리로써 년간(年間) 2-3회(回) 예확(刈穫)이 좋겠고 연료(燃料)로 사용(使用)코저 할때는 생산량(生産量)이 많은 싸리로써 년일회(年一回) 예확(刈穫)이 좋은 것으로 판단(判斷)되었다.

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한국 소아·청소년의 타액과 치태 내 치주 병원균 출현율 (Prevalence of Periodontopathogens in Saliva and Plaque of Korean Children and Adolescents)

  • 최혜진;김재환;이대우;양연미;김재곤
    • 대한소아치과학회지
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    • 제43권1호
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    • pp.8-16
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    • 2016
  • 소아에서 치주 병원균의 조기 집락은 향후 치주 질환으로 발전될 위험 인자로 작용할 수 있으므로 원인균의 조기 진단과 예방이 최선의 치료라 할 수 있다. 이 연구는 치주적으로 건강한 어린이와 청소년에서 타액과 치태를 채취하고, PCR을 사용하여 주요 치주 질환 원인균 5종의 분포 정도를 조사하였으며, 나아가 타액과 치태의 검출 차이를 비교 분석하였다. A. actinomycetemcomitans와 P. intermedia는 고등학생(만17세)에서, T. forsythia와 F. nucleatum는 초등학생(만8세)에서 가장 많이 검출되었다. 반면, P. gingivalis는 모든 대상에서 발견되지 않았다. 그리고 치은연상치태보다 타액에서 유의하게 검출이 더 잘 된 것으로 나타났다. 치주 병원균이 상대적으로 더 많이 검출된 청소년기에서는 치주 질환 예방을 위한 구강위생교육이 더욱 강화될 필요가 있으며, 치주 질환의 조기진단 및 관련 연구에 있어서 타액은 보다 유용한 도구가 될 것이라 사료된다.

근로자의 구강환경요인에 따른 구강세균 발생의 위험요인 (Risk factors for the development of oral bacteria in workers according to oral environment)

  • 홍민희
    • 한국산학기술학회논문지
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    • 제17권6호
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    • pp.537-545
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    • 2016
  • 본 연구는 구강환경요인을 살펴보고, 구강환경이 구강세균 검출에 미치는 위험요인 알아보고자 시행하였다. 연구기간은 2015년 2월 15일~ 2월 28일까지 성인 근로자 60명을 대상으로 조사하였다. 연구변수로는 안정시, 자극시 타액분비율, 타액완충능, 타액 pH, 설배와 설하 구강건조도, 구취 설태량을 측정하였다. 구강세균의 검출 유무를 확인하기 위하여 안정시 타액분비율의 gDNA를 추출하여 균을 검출하였다. 그 결과, S.mutans균은 흡연자 15배, 자극시, 안정시 타액분비율 1.3~1.6배 위험도가 더 높았으며, P.intermedia는 흡연자 13배, 설하 구강건조도 4.3배, 설태량 4배 TM7은 설하 구강건조도 5.5배 더 높은 위험도를 나타냈다. 구강 내 세균을 줄이기 위해서는 타액분비율을 촉진시키는 습관을 형성하고, 금연교육을 시행함이 중요하다고 본다. 또한 구강위생이 깨끗하고 관리가 잘되는 성인에서는 구강세균이 많이 줄어들고, 구강질환을 좀 더 예방할 수 있을 것이라 기대된다.

한국인 성인성 치주염 환자에서 16S rRNA 분석을 이용한 치은연하치태 세균 분포도 조사 (The detection of subgingival plaque microflora using 16S rRNA analysis in Korean adult periodontitis)

  • 박성희;김소영;최성호;채중규;김종관;조규성
    • Journal of Periodontal and Implant Science
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    • 제28권4호
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    • pp.691-703
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    • 1998
  • The 16S rRNA analyzing method is a bacterial identification method that is useful in identifying bacteria which is difficult to do by other means. The following 7 types of bacteria which are Treponema, A. actinomycetemcomitans, P. gingivalis, Fusobacterium, B. forsythus, P. intermedia, P. micros were evaluated in order to study their distribution among patients with adult periodontitis. The 16S rRNA analyzing method was used to compare bacterial distribution among 3 groups. Subgingival plaque acquired from the affected sites(pocket depth ${\geq}6mm$) of 29 patients with adult periodontitis were grouped as the experimental group while plaque from the non-affected sites(pocket depth ${\leq}3mm$) were grouped as control 2 and finally plaque acquired from students with healthy periodontal tissues were grouped as control 1. The results are as follows ; 1. The distribution of Treponema was 12.5% for control 1, 21.4% for control 2 and 75.4% for the experimental group. For A. actinomycetemcomitans the distribution was 0.5%, 19.0%, 44.4% in respect to the order of groups mentioned above. P.gingivalis showed 10.5%, 43.1%, 94.0% distribution, Fusobacterium 33.0%, 48.3%, 81.0% distribution, B. forsythus 9.5%, 17.2%, 65.9% distribution, P. intermedia 1.0%, 12.1%, 26.3% distribution and finally P. micros 5.0%, 19.0%, 48.7% respectively. In all 7 types of bacteria, the experimental group showed higher bacterial distribution compared to the other two groups with statistically significant difference. 2. In the case of Treponema, A. actinomycetemcomitans, gingivalis,Fusobacterium, B. forsythus, P. intermedia, P. micros showed significant difference between control 1 and 2. These results suggest that the 16S rRNA analyzing method which was applied on Koreans for the first time could be utilized and useful in finding potential pathogens of periodontal disease.

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항균작용을 가진 수종 한약재의 구취억제 효과 (Deodorizing Effect of Several Antibacterial Medicinal Herbs on Oral Malodor)

  • 김현경;박재우;윤성우;류봉하;김진성
    • 대한한의학회지
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    • 제31권4호
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    • pp.151-163
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    • 2010
  • Objective: We investigated the oral malodor inhibitory effect of Scutellariae Radix (SR), Phellodendri Cortex (PC), Moutan Cortex (MTC) and Magnoliae Cortex (MGC) for the development of a gargle solution. Methods: 1. Against P. gingivalis and Pr. intermedia, the minimal bactericidal concentration (MBC) and the change of viable cells that were exposed to 1% each herbal extract were observed. 2. Deodorizing activity of 2% herbal extract and Garglin $Mint^{(R)}$ against methyl mercaptan were evaluated by gas chromatography (GC). 3. We used the salivary sediment system (SSS) as the malodor model. 4. The clinical examination was repeated 3 times by 2 subjects by $Halimeter^{(R)}$. Baseline VSC of each subject was measured. Then, the control subject gargled with cysteine for 30 sec. After 4 min, subjects would gargle for 30 seconds with herbal extracts (2%) and Garglin $Mint^{(R)}$. Subsequently, the concentration of VSC was measured at 0, 4, 8, 12, 16, 20, 40 and 60 minutes. Results: 1. Against P. gingivalis, MBC of SR, PC and MTC was 0.1%, and MBC of MGC was 1%. Removal time of P. gingivalis was as follows; 5 hr in MGC, 24 hr in SR and PC, and 48 hr in MTC. Against Pr. intermedia, MBC of SR and PC was 0.5%, and MBC of MTC, MGC was 1%. Removal time of Pr. intermedia was as follows; 5 hr in MTC and 24 hr in SR, PC and MGC. 2. Deodorizing effect of herbal extracts against methyl mercaptan was as follows; MGC and MTC had 100%, SR had 82.22%, PC had 66.60%, Garglin $Mint^{(R)}$ had 40.54%. 3. In the experiment using SSS, PC and MTC had statistically significant malodor-inhibitory effects (p<.05). 4. In the clinical examination, PC and MGC had statistically significant inhibitory effects at every elapsed time compared to the control subject. MTC had that until 40 min. SR had that at 0, 4, 8, 20, and 60 min. Conclusions: SR, PC, MTC and MGC have an antibacterial effect and the chemical removable activity of the oral malodor caused by VSC. These four herbs could have potential as effective anti-malodor agents.

구강함수제 개발을 위한 오매, 비파엽, 오가피, 백지의 구취억제효과 연구 (Study on Deodorizing Effects of Mume Fructus, Eriobotryae Folium, Acanthopanacis Cortex and Angelicae Dahuricae Radix for the Development of a Gargle Solution)

  • 장선영;박재우;윤성우;류봉하;김진성
    • 대한한의학회지
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    • 제31권4호
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    • pp.115-128
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    • 2010
  • Objectives: The aim of this study was to investigate deodorizing effectsof medicinal herbs (Mume Fructus, Eriobotryae Folium, Acanthopanacis Cortex, Angelicae Dahuricae Radix) for development of a gargle solution. Methods: 1. The antimicrobial effects of medicinal herbs were evaluated with the minimal bactericidal concentration (MBC) and the change of the number of viable cells in the herb extracts(1%) for 48 hrs against P. gingivalis 2561 and Pr. intermedia ATCC 25611. 2. Deodorizing activity of each herb and Garglin $Mint^{(R)}$gainst methyl mercaptan were analyzed by gas chromatography (GC). 3. We used the malodor modeling of the salivary sediment system with a Halimeter. 4. In the preliminary clinical study, the baseline concentration of VSC in the oral cavity of each subject was measured by Halimeter. Subjects would gargle for 30 seconds with cysteine. After 4 minutes subjects would gargle for 30 seconds with Garglin and herb extracts (2%). Subsequently, concentration of VSC were measured at 0, 4, 8, 12 and 20 minutes. Results: 1. MBC of Mume Fructusfor P. gingivalis 2561 was determined to be <1% and MBCs of Eriobotryae Folium for P. gingivalis 2561 and Pr. intermedia ATCC 25611 were determined to be <2% and <1%, respectively. Mume Fructus (1%) completely suppressed the P. gingivalis cell viability from 5 hrs and Eriobotryae Folium (1%) completely suppressed the Pr. intermedia cell viability from 48 hrs. 2. In GC analysis, deodorizing activities were 91.54% with Mume Fructus, 87.97% with Eriobotryae Folium, 100% with Acanthopanacis Cortex, 72.36% with Angelicae Dahuricae Radix and 40.54% with Garglin $Mint^{(R)}$. 3. In malodor modeling of the salivary sediment system, each of the medicinal herbs had significantly inhibitory effect on malodor formation (p<0.05). 4. In the preliminary clinical study, the concentration of VSC of the herb groups was significantly lower than of the control group, but not in Garglin $Mint^{(R)}$. Conclusions: Mume Fructus, Eriobotryae Folium, Acanthopanacis Cortex and Angelicae Dahuricae Radixhave deodorizing activities and potential as an effective mouthwash against oral malodor.