• BMB Reports
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• v.40 no.3
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• pp.315-324
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• 2007

The novel $\alpha$-amylase purified from locally isolated strain, Bacillus sp. KR-8104, (KRA) (Enzyme Microb Technol; 2005; 36: 666-671) is active in a wide range of pH. The enzyme maximum activity is at pH 4.0 and it retains 90% of activity at pH 3.5. The irreversible thermoinactivation patterns of KRA and the enzyme activity are not changed in the presence and absence of $Ca^{2+}$ and EDTA. Therefore, KRA acts as a $Ca^{2+}$-independent enzyme. Based on circular dichroism (CD) data from thermal unfolding of the enzyme recorded at 222 nm, addition of $Ca^{2+}$ and EDTA similar to its irreversible thermoinactivation, does not influence the thermal denaturation of the enzyme and its Tm. The amino acid sequence of KRA was obtained from the nucleotide sequencing of PCR products of encoding gene. The deduced amino acid sequence of the enzyme revealed a very high sequence homology to Bacillus amyloliquefaciens (BAA) (85% identity, 90% similarity) and Bacillus licheniformis $\alpha$-amylases (BLA) (81% identity, 88% similarity). To elucidate and understand these characteristics of the $\alpha$-amylase, a model of 3D structure of KRA was constructed using the crystal structure of the mutant of BLA as the platform and refined with a molecular dynamics (MD) simulation program. Interestingly enough, there is only one amino acid substitution for KRA in comparison with BLA and BAA in the region involved in the calcium-binding sites. On the other hand, there are many amino acid differences between BLA and KRA at the interface of A and B domains and around the metal triad and active site area. These alterations could have a role in stabilizing the native structure of the loop in the active site cleft and maintenance and stabilization of the putative metal triad-binding site. The amino acid differences at the active site cleft and around the catalytic residues might affect their pKa values and consequently shift its pH profile. In addition, the intrinsic fluorescence intensity of the enzyme at 350 nm does not show considerable change at pH 3.5-7.0.

• Therapeutic Science for Rehabilitation
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• v.1 no.2
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• pp.5-13
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• 2012

The purpose of the research study is to confirm in effectiveness of somatosensory stimulation and to propose ideal training strategy for functional recovery of stroke patients. Through the previous literatures, our study investigated to the advantages and disadvantages in electrical somatosensory stimulation for stroke patients. Also, our study suggested to applicable strategies and confirmed to growth direction about new somatosensory stimulation therapy for functional recovery in stroke patients. Result of research study, although many study demonstrated to the effectiveness about somatosensory stimulation therapy for stroke patients, many therapists have experienced many difficulties in somatosensory stimulation application for stroke patients in rehabilitation environments. Thus, few have the therapeutic tools for somatosensory or specific sensory input. However, apparently the previous literatures showed that effectiveness of somatosensory stimulation on functional recovery of patients with brain damage. In conclusion, we can be confirmed that an ideal somatosensory stimulation program is very effective in promoting recovery and the integrity of the somatosensory pathway of stroke patients. Furthermore, we anticipate that using the customized mechanical interface provides to positive effects in rehabilitation of patients with brain damage.

• The KIPS Transactions:PartC
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• v.12C no.6 s.102
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• pp.773-780
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• 2005

Recently, the telecommunication network is in a transition toward the BcN (Broadband convergence Networks) that integrates the Internet, the telecommunication and the broadcasting. The BcN makes it possible to provide of 4he third-party application by using the Open API The Parlay X API is an example of the Open API and the Parlay X API is grafted in the Web Service and is abstracted from the Parlay API. So, the Parlay X API does not only have advantages of a Web Service, but also disadvantages of the Web Service. The main disadvantage of the Web Service is that it does not support the any QoS mechanism. In this paper, we propose the architecture that solves the reliability among the QoS issues for the Web Service. The proposed mechanism adopts the Asynchronous architecture for the reliable Web Service and improves the performance of the retransmission between the client and the server using the expected response time. We perform the performance evaluation through the simulation in older to compare with the existing mechanism. Consequently, we can confirm that the performance of the proposed mechanism performs better than the existing mechanism.

• Journal of the Korean Society for Library and Information Science
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• v.25
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• pp.83-150
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• 1993

Traditionally, the emphasis of the information services had been placed on the information provider's knowledge of information sources or information systems. However, in view of the increasing complexity of the information environment. and changes taking place in information professions the evaluation of the information services has callded for further research in interpersonal communication aspects of the librarian user interface especially that which links to user satisfaction. This experimental study was carried out for the purpose of exploring the implications of nonverbal behaviors in measuring user satisfaction in the light of the fact that the meaning of nonverbal clues includes more than verbal communication especially in dealing with variable barriers even though they operate at low levels of awareness. In this sense, among the wide range of nonverbal communication areas in particular kinesics, artifacts and the proximics were examined to suggest alternative model which can be applied effectively in our specific set of information work environment. Two major university libraries and two research libraries in Korea were chosen and visited for the anlysis and per each refernce/information interview the librarian's nonverbal behaviors were checked on the observer's checklist and the qustionnaires completed by the user at a time. On the basis of these data firstly, the relation of user satisfaction concerned with the librarian's nonverbal communication to user satisfaction with information services was analyzed and the possible communication barriers were investigated. In addition, the effect of training the information librarian with positive nonverbal communication skills on the user's satisfaction was analyzed in a SAS computer program in the area of kinesics. On the other hand, the effect of other nonverbal behaviors such as proximics and artifacts known very important clues to improving a complex human interaction was discussed along with their experimental results. In conclusion, it was found that most of users were not sensitive to the importance of nonverbal behaviors exposed by the information librarian, but it was proved that the ultimate user satisfaction and the satisfaction of the librarian's nonverbal behaviors are correlated positively, Moreover, nonverbal behaviors displayed by the trained librarian toward the user had a considerable effect on the user's evalution of information services compared with those by the untrained. These findings imply that in order to control the librarian's nonverbal behaviors and to help the user overcome his barriers the information librarian as a specialist needs to assure and modify willilingly his communication behavior. Without making effective use of such nonverbal communication, information librarian could not expect to meet the user's information needs either. However, when these positive nonverbal behaviors are applied it would be highly desirable to be aware of cultural and contextual differences. With regard to increasing information services success the applicable nonverbal communication model for library management and education can be suggested as below.

• Journal of KIISE:Computing Practices and Letters
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• v.9 no.6
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• pp.699-711
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• 2003

Home networking and Internet control technologies must satisfy three requirements with a view to being applied to home for raising the qualify of human life. Firstly, the cost of adding home networking ability to the traditional appliance must be minimized. Secondly, the mutual accessibility between home network and Internet must be guaranteed in the various Internet service environment of home. Finally, communication must be possible between the home network and the Internet that use different communications protocols. This paper proposes the ARCS (Appliance Remote Control System) that controls appliances based on LnCP (Living network Control Protocol) via the Internet. LnCP is the most cost-effective network and control protocol for home network. ARCS guarantees the accessibility from the Internet to home network and provides users with the virtual interface designed for controlling appliances using the Internet application program. In addition, this paper proposes AICS (Appliance Internet Control Specification). AICS, is based on LnCP, defines the message structure and control commands for the Internet control communication. ARCS and home gateway are developed to meet AICS.

• Journal of KIISE:Software and Applications
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• v.32 no.12
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• pp.1271-1282
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• 2005

Recently, PC-based control is incredibly developed in the industrial control field, but it is difficult for PLC programming in PC. Therefore, I need to develop the softeware PLC, which support the international PLC programming standard(IECl131-3) and can be applied to diverse control system by using C language. In this paper, I have developed the ISPLC(Intelligent Agent System based Software Programmable Logic Controller). In ISPLC system, LD programmed by a user which is used over $90\%$ among the 5 PLC languages, is converted to IL, which is one of intermediate codes, and IL is converted to the standard C rode which can be used in a commercial editor such as Visual C++. In ISPLC, the detection of logical error in high level programming(C) is more eaier than PLC programming itself The study of code conversion of LD->IL->C is firstly tried in the world as well as KOREA. I developed an execution engine with a good practical application. To show the effectiveness of the developed system, 1 applied it to a practical case, a real time traffic control(RT-TC) system. ISPLC is minimized the error debugging and programming time owing to be supported by windows application program.

• Journal of Biomedical Engineering Research
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• v.19 no.3
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• pp.251-260
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• 1998

The Holter monitoring system is a widely used noninvasive diagnostic tool for ambulatory patient who may be at risk from latent life-threatening cardiac abnormalities. In this paper, we design a high performance intelligent holter monitoring system which is characterized by the small-sized and the low-power consumption. The system hardware consists of one-chip microcontroller(68HC11E9), ECG preprocessing circuit, and flash memory card. ECG preprocessing circuit is made of ECG preamplifier with gain of 250, 500 and 1000, the bandpass filter with bandwidth of 0.05-100Hz, the auto-balancing circuit and the saturation-calibrating circuit to eliminate baseline wandering, ECG signal sampled at 240 samples/sec is converted to the digital signal. We use a linear recursive filter and preprocessing algorithm to detect the ECG parameters which are QRS complex, and Q-R-T points, ST-level, HR, QT interval. The long-term acquired ECG signals and diagnostic parameters are compressed by the MFan(Modified Fan) and the delta modulation method. To easily interface with the PC based analyzer program which is operated in DOS and Windows, the compressed data, that are compatible to FFS(flash file system) format, are stored at the flash memory card with SBF(symmetric block format).

• Journal of the Institute of Convergence Signal Processing
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• v.8 no.1
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• pp.58-62
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• 2007

Digital communication is important for reliability and mobilization of the multi-channel communication systems. Transmitting and receiving data for the mobilization should be possible in anywhere and in anytime. And this system must be designed light weight small size and low power. One are essential technology for implementing the mobile wireless communication system on the age of ubiquotos. Requirements in constructing such communication field are followings. At first data transmitting and receiving should be carried out by a simple command. Second, the device should be designed as hand-hold type and low power consumption. Third, data communication should be reliable. As one of examples, car to car system which is popular in the market is introduced here, All traffic information in highway is transmitted from one car to another by using this system which can prevent possible traffic accident. This paper shows the design of a digital data communication system with CC1020 chip. This CC1020 makes easy frequency selection and easy switch from the transmit mode to the receive mode by simple setting of a memory register in the chip. The transmit power of this system is designed 10dBm and its communication range is about 100m. The power supplied this system is 3V considered as low power. The sleep mode can be easily entered during transmit mode or receive mode. We shows the program algorithm of CC1020 and interface circuit between MCU and CC1020. We shows the Photo of the CC1020 Module and Atmega128 Module.. We analysed the receiver rate with this system.

• Korean Journal of Food Science and Technology
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• v.19 no.6
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• pp.550-555
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• 1987

Strain gauges attached on the Bourdon tube and load cell were used as the sensors for measuring the vacuum pressure in drying chamber and the weight loss of Shiitake mushrooms respectively. The vacuum drying system was interfaced further with the Bear II microcomputer. The interface devices used were built with such IC chips as MC 6821, ADC 0809, SN 74244 and SN 7424. The relationship between readings of vacuum gauge (P, mmHg) and digital outputs (D) from the microcomputer was represented by P =3.08 D-13.4875(r=0.9999). The weights of drying sample (W) were also related with the digital outputs (D) by W=0.4076 D-6.4762 (r=0.9999). During the vacuum drying of Shiitake mushrooms. the data on pressure and weight were recorded at regular intervals using an acquisition program on the microcomputer system. The Page model was fitted well to the drying data of Shiitake mushrooms. resulting in the following empirical equations : $(M-M_e)/(M_o-M_e)=\exp(-0.1569t^{1.0048})$ at 400 mm Hg up to 14 hours and $(M-M_e)/(M_o-M_e)=\exp(-0.1385_t^{1.2688})$ at 600 mm Hg up to 8 hours.

• Journal of the Korean Geosynthetics Society
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• v.9 no.1
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• pp.47-57
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• 2010

Granular soils having a large particle size have been used as a filling material in the construction of foundation, harbor, dam, and so on. Consequently, the shear behavior of this granular soil plays a key role in respect of stability of structures. For example, soil particle crushing occurring at the interface between structure and soil and/or within soil mass can cause a disturbance of ground characteristics and consequently induce issues in respect of stability of structures. In order to investigate the shear behavior according to an existence and nonexistence of particle crushing, numerical analyses were conducted by using the DEM (Discrete Element Method)-based software program PFC2D (Particle Flow Code). By dividing soil particle bonding model into crushing model and noncrushing model, total four particle bonding models were simulated and their results were compared. Noncrushing model included one ball model and clump model, and crushing model included cluster model and Lobo-crushing model. The combinations of soil particle followed the research results of Lobo-Guerrero and Vallejo (2005) which were composed of eight circles. The results showed that the friction angle was in order of clump model > cluster model > one ball model. The particle bonding model compared to one ball model and noncrushing model compared to crushing model showed higher shear strength. It was also concluded that the model suggested by Lobo-Guerrero and Vallejo (2005) is not appropriate to simulate the soil particle crushing.