• 제목/요약/키워드: Interest Stability

검색결과 455건 처리시간 0.032초

대장균에서 Selenocysteine을 가지고 있는 Formate Dehydrogenase H의 최적화 생산 (Optimized Production of Selenocysteine Containing Formate Dehydrogenase H in Escherichia coli)

  • 사영승;김용환
    • KSBB Journal
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    • 제26권3호
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    • pp.189-192
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    • 2011
  • Much interest has been recently focused on the production of large quantities of hydrogen, due to its potential importance in our economy and needs in the petroleum and chemical industries. Formate dehydrogenase H (FDH-H) from Escherichia coli containing selenocysteine that oxidizes formate to carbon dioxide with the release of hydrogen is a component of the anaerobic formate hydrogen-lyase complex of E. coli. To make full use of FDH-H, we need effective expression condition. In this approach, we investigated the effect of pH on FDH-H stability and observed the effect of selenite and formate concentration on the activity of FDH-H. Additionally, coexpression of selenocysteine insertion genes were tried to improve the expression of FDH-H. The highest level of FDH-H expression was achieved by coexpression of selenocysteine insertion genes (pSUABC) as well as by the addition of $10\;{\mu}M$ selenite and 10 mM formate. At this optimized condition, a 2.6 fold elevation of expression of FDH-H was achieved.

외륜 이동로봇의 균형제어 알고리즘 (Balancing Control Algorithm for a Single-Wheeled Mobile Robot)

  • 이현탁;박희재
    • 한국생산제조학회지
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    • 제26권1호
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    • pp.144-149
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    • 2017
  • There have been lots of interest on service and entertainment robots. To ensure that robots work in harmony with humans, their stability and compactness are some of the key issues. Obviously, robots with fewer wheels occupy a smaller floor area compared to those with more wheels. In addition, robots with fewer wheels, whose posture stabilities are maintained by feedback control, are stable even under larger accelerations and/or higher locations of the center of mass. To facilitate controller design, it is assumed that both pitch and roll dynamics are decoupled. The dynamic equations of motion for the proposed robot are derived from the Euler-Lagrange equation. To obtain the optimal balancing control law, linear quadratic regulator control methods are applied to the linearized dynamic equations. Simulation and experimental results verify the effectiveness and performance of the proposed balancing control algorithm for a single-wheeled mobile robot.

통합 샤시제어 시스템 개발을 위한 시뮬레이션 환경 구축 (A Simulation Environment Development for Global Chassis Control System of Vehicles)

  • 황태훈;박기홍;허승진;이민수;이규훈;기승관
    • 한국정밀공학회:학술대회논문집
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    • 한국정밀공학회 2005년도 춘계학술대회 논문집
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    • pp.1095-1098
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    • 2005
  • Most electronic chassis control systems until today have been designed with optimization on its own performance. However, According to the increase of the interest regarding a vehicle safety and development of information technique, the integration technique of current chassis systems is being emphasized. Each enterprise proposed it with name of GCC(Global Chassis Control) or UCC(Unified Chassis Control). This study realizes control algorithm of suspension and brake by using the vehicle model of low degree of freedom as the primary stage of realization of integrated chassis control system. The proposed algorithm build the simulation environment connected to the CarSim having full vehicle model of 27 degree of freedom for raising the thrust of results

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Fluorescence Microscopy of Condensed DNA Conformations of Bacterial Cells

  • Suleymanoglu, Erhan
    • Journal of Microbiology
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    • 제40권4호
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    • pp.319-326
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    • 2002
  • Cellular DNA in prokaryotes is organized in nucleic acid-protein self-assemblies referred to as the nucleoid. The physical forces responsible for its stability inside the poor solvent properties of the cytoplasm and their functional implications are not understood. Studies on the organisation and functioning of the cytosol of cells largely rely on experimental protocols performed in highly dilute solutions using biochemically purified molecules, which is not a reliable substitute for the situation existing in vivo. Our current research interest is focused on the characterization of biological and physical forces determining the compaction and phase separation of DNA in Escherichia coli cytoplasm. We have emphasized the effect of excluded volume in solutions with high macromolecular concentrations (macromolecular crowding) upon self-association patterns of reactions. The prokaryotic cytosol was simulated by addition of inert polymer polyethylene glycol (PEG) (average molecular weight 20000), as an agent which afterwards facilitates the self-association of macromolecules. Fluorescence microscopy was used for direct visualization of nucleoids in intact cells, after staining with DAPI (4',6-diamidino-2-phenylindole dihydrochloride). Addition of the crowding agent PEG 20,000, in increasing concentrations generated progressively enhanced nucleoid compaction, the effect being stronger in the presence of 0.2 M NaCl and 5 mM MgCl$\_$2/. Under these conditions, the nucleoids were compacted to volumes of around 2 ㎛$\^$3/ or comparable sizes with that of living cells.

Application of a Robust Fuzzy Sliding Mode Controller Synthesis on a Buck-Boost DC-DC Converter Power Supply for an Electric Vehicle Propulsion System

  • Allaoua, Boumediene;Laoufi, Abdellah
    • Journal of Electrical Engineering and Technology
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    • 제6권1호
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    • pp.67-75
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    • 2011
  • The development of electric vehicle power electronics system control, composed of DC-AC inverters and DC-DC converters, attract much research interest in the modern industry. A DC-AC inverter supplies the high-power motor torques of the propulsion system and utility loads of electric vehicles, whereas a DC-DC converter supplies the conventional low-power and low-voltage loads. However, the need for high-power bidirectional DC-DC converters in future electric vehicles has led to the development of many new topologies of DC-DC converters. The nonlinear control of power converters is an active research area in the field of power electronics. This paper focuses on the use of the fuzzy sliding mode strategy as a control strategy for buck-boost DC-DC converter power supplies in electric vehicles. The proposed fuzzy controller specifies changes in control signals based on the surface and knowledge on surface changes to satisfy the sliding mode stability and attraction conditions. The performance of the proposed fuzzy sliding controller is compared to that of the classical sliding mode controller. The satisfactory simulation results show the efficiency of the proposed control law, which reduces the chattering phenomenon. Moreover, the obtained results prove the robustness of the proposed control law against variations in load resistance and input voltage in the studied converter.

원발성 흉벽 골종양24례 보고 (Primary Bony Thorax Tumor Report of 24 cases)

  • 조건현;이홍균
    • Journal of Chest Surgery
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    • 제18권1호
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    • pp.69-74
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    • 1985
  • Most clinicians have taken a lot of interest in tumors arising from the bony thorax because not only of their rarity and predictable diagnosis which could be reflected as a unique radiologic shadow but also variable surgical modes for maintenance of chest wall stability encountered after en-bloc resection. By the retrospective review, we have analyzed 24 cases of primary bony thorax tumors which were experienced and surgically treated at the St. Mary`s hospital of Catholic Medical College from Jan. 1969 to Sept. 1984. The results are as follows: 1. Age incidence was evenly distributed through all decades and the male-female ratio is 15:9. 2. 16 cases out of 24 were benign tumors and the commonest one of which was fibrous dysplasia. 3. Remaining 8 cases were malignant tumors and among which osteogenic sarcoma was the commonest one. 4. The majority of tumors [22/24] were developed from the rib and the remains were from the sternum. 5. Common manifestation were palpable mass or swelling and localized tenderness. 6. Various kinds of operative procedure were underwent: single resection of rib including tumor,14 cases; multiple resection of ribs with chemotherapy or myoplasty, 2 cases; en-bloc resection of the chest wall and reconstructive procedure, 5 cases; partial resection of sternum, 1 case; bone biopsy and chemotherapy, 2 cases.

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실내공간에 있어서 식물 이용 방법과 선호도 분석에 관한 연구 (A Study on the Method of Using Plants and Preference Analysis for Interior Space)

  • 김태환;한승원
    • 한국실내디자인학회논문집
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    • 제11호
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    • pp.31-37
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    • 1997
  • This study intens to provide available guideline of useful plants in interior and be used by designer. It focuses on evaluating the character of plants, the method of installation and the form of composition for suitable design of using plants. The study finds out that using plants in interior are necessary positively by 94% respondents. And results of factor analysis are that plants are needed for emothional stability(factor 1), visual charming(factor 2), and educational effection(factor 3). According to job, the group of students is visual charming (P=0.0037), the public servants and housewives is educational effection(p=0.0029). The most available space of plants as follows : Department(30.5%), Hotell(26.3%), House and Restuarant(12.7%). It has been identified that the public institution and commercial space are much indused plants. The most indused plants in house is ordered that the veranda, the living room, the entrance. This result is concerned about relation of natural light plants. The repondants reply that the favorite arrangement style is the corner form(63%), the center garden form(52.1%), the large planter(19.7%), the planter box(11.3%). They respond that they are more favorite light green color than dark, and mono color than mixture color. Finally, interior Designers should be understood the functional and aesthetic elements and the special interest about plants for developing desigv.

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태양광 기반의 가변속 하이브리드 시스템을 위한 직류 전압 제어 (DC-Voltage Regulation for Solar-Variable Speed Hybrid System)

  • 니이테게카 기디언;이경규;최재호;송유진
    • 전력전자학회논문지
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    • 제21권3호
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    • pp.231-237
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    • 2016
  • Recently, the interest in DC systems to achieve more efficient connection with renewable energy sources, energy storage systems, and DC loads has been growing extensively. DC systems are more advantageous than AC systems because of their low conversion losses. However, the DC-link voltage is variable during operation because of different random effects. This study focuses on DC voltage stabilization applied in stand-alone DC microgrids by means of voltage ranges, power management, and coordination scheme. The quality and stability of the entire system are improved by keeping the voltage within acceptable limits. In terms of optimized control, the maximum power should be tracked from renewable resources during different operating modes of the system. The ESS and VSDG cover the power shortage after all available renewable energy is consumed. Keeping the state of charge of the ESS within the allowed bands is the key role of the control system. Load shedding or power generation curtailment should automatically occur if the maximum tolerable voltage variation is exceeded. PSIM-based simulation results are presented to evaluate the performance of the proposed control measures.

그래핀 플레이크 크기에 따른 전기 이중층 커패시터용 전극의 전기화학적 특성 (Electrochemical Properties of EDLC Electrodes with Diverse Graphene Flake Sizes)

  • 유혜련
    • 한국전기전자재료학회논문지
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    • 제31권2호
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    • pp.112-116
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    • 2018
  • Electric double layer capacitors (EDLCs) are promising candidates for energy storage devices in electronic applications. An EDLC yields high power density but has low specific capacitance. Carbon material is used in EDLCs owing to its large specific surface area, large pore volume, and good mechanical stability. Consequently, the use of carbon materials for EDLC electrodes has attracted considerable research interest. In this paper, in order to evaluate the electrochemical performance, graphene is used as an EDLC electrode with flake sizes of 3, 12, and 60 nm. The surface characteristic and electrochemical properties of graphene were investigated using SEM, BET, and cyclic voltammetry. The specific capacitance of the graphene based EDLC was measured in a 1 M $TEABF_4/ACN$ electrolyte at the scan rates of 2, 10, and 50 mV/s. The 3 nm graphene electrode had the highest specific capacitance (68.9 F/g) compared to other samples. This result was attributed to graphene's large surface area and meso-pore volume. Therefore, large surface area and meso-pore volume effectively enhances the specific capacitance of EDLCs.

발광층($Alq_3$)의 두께 변화에 따른 OLED의 효율 특성 연구 (Efficiency Properties of OLED Depending on Thickness Variation of Emission Layer($Alq_3$))

  • 박준우;최규채;김동은;김병상;권영수
    • 대한전기학회:학술대회논문집
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    • 대한전기학회 2009년도 제40회 하계학술대회
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    • pp.1236_1237
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    • 2009
  • Organic light emitting diode (OLED) is currently the focus of intense interest in the field of photonics. It is attractive for the in low-operating voltage, low power consumption, easy fabrication and low cost. A typical OLED consists of one or more organic layers sandwiched between a high work function anode, such as indium tin oxide (ITO), and a low work function cathode such as Ca, Mg:Ag, and Al. Tris-(8-hydroxy)quinolinealuminum ($Alq_3$) has taken a prominent position in the development of OLED due to its relative stability as an electron transporting and emitting material. We investigated an efficiency improvement of the OLED depending on thickness variation of $Alq_3$.

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