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The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Optimized Production of Selenocysteine Containing Formate Dehydrogenase H in Escherichia coli

대장균에서 Selenocysteine을 가지고 있는 Formate Dehydrogenase H의 최적화 생산

  • Sa, Young-Seung (Department of Chemical Engineering, Kwangwoon University) ;
  • Kim, Yong-Hwan (Department of Chemical Engineering, Kwangwoon University)
  • Received : 2011.01.27
  • Accepted : 2011.06.02
  • Published : 2011.06.30
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Abstract

Much interest has been recently focused on the production of large quantities of hydrogen, due to its potential importance in our economy and needs in the petroleum and chemical industries. Formate dehydrogenase H (FDH-H) from Escherichia coli containing selenocysteine that oxidizes formate to carbon dioxide with the release of hydrogen is a component of the anaerobic formate hydrogen-lyase complex of E. coli. To make full use of FDH-H, we need effective expression condition. In this approach, we investigated the effect of pH on FDH-H stability and observed the effect of selenite and formate concentration on the activity of FDH-H. Additionally, coexpression of selenocysteine insertion genes were tried to improve the expression of FDH-H. The highest level of FDH-H expression was achieved by coexpression of selenocysteine insertion genes (pSUABC) as well as by the addition of $10\;{\mu}M$ selenite and 10 mM formate. At this optimized condition, a 2.6 fold elevation of expression of FDH-H was achieved.

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References

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