• KSBB Journal
• /
• v.24 no.1
• /
• pp.30-40
• /
• 2009

Statistical optimization of the production medium was carried out in order to find an optimal medium composition in itaconic acid fermentation process. Itaconic acid utilized in the manufacture of various synthetic resins is a dicarboxylic acid biosynthesized by fungal cells of Aspergillus terreus in a branch of the TCA cycle via decarboxylation of cis-aconitate. Through OFAT (one factor at a time) experiments, six components (glucose, fructose, sucrose, soluble starch, soybean meal and cottonseed flour) were found to have significant effects on itaconic production among various carbon- and nitrogen-sources. Hence, using these six factors, interactive effects were investigated via fractional factorial design, showing that the initial concentrations of sucrose and cottonseed flour should be high for enhanced production of itaconic acid. Furthermore, through full factorial design (FFD) experiments, negative effects of $KH_2PO_4$ and $MgSO_4$ on itaconic acid biosynthesis were demonstrated, when excess amounts of the each component were initially added. Based on the FFD analysis, further statistical experiments were conducted along the steepest ascent path, followed by response surface method (RSM) in order to obtain optimal concentrations of the constituent nutrients. As a result, optimized concentrations of sucrose and cottonseed flour were found to be 90.4g/L and 53.8g/L respectively, with the corresponding production level of itaconic acid to be 4.36 g/L (about 7 fold higher productivity as compared to the previous production medium). From these experimental results, it was assumed that optimum ratio of the constituent carbon (sucrose) and nitrogen (cottonseed flour) sources was one of the most important factors for the enhanced production of itaconic acid.

• Restorative Dentistry and Endodontics
• /
• v.24 no.1
• /
• pp.67-75
• /
• 1999

The use of composite restorative materials is established due to continuing improvements in the materials and restorative techniques. Composite resins are widely used for the restoration of cervical lesions because of esthetics, good physical properties and working time. There are several types of cavity design for class V composite resin filling, but inappropriate cavity form may affect bonding failure, microleakage and fracture during mastication. Cavity preparations for composite materials should be as conservative as possible. The extent of the preparation is usually determined by the size, shape, and location of the defect. The design of the cavity preparation to receive a composite restoration may vary depending on several factors. In this study, 5 types of class V cavity were prepared on each maxillary central incisor. The types are; 1) V-shape, 2) round(U) shape, 3) box form, 4) box form with incisal bevel and 5) box form with incisal bevel and grooves for axial line angles. After restoration, in order to observe the concentration of stress at bonding surfaces of teeth and restorations, developing a 2-dimensional finite element model of labiopalatal section in tooth, surrounding bone, periodontal ligament and gingiva, based on the measurements by Wheeler, loading force from direction of 45 degrees from lingual side near the incisal edge was applied. This study analysed Von Mises stress with SuperSap finite element analysis program(Algor Interactive System, Inc.). The results were as follows : 1. Stress concentration was prevalent at tooth-resin bonding surface of cervical side on each model. 2. In model 2 without line angle, stress was distributed evenly. 3. Preparing bevel eliminated stress concentration much or less at line angle. 4. Model with round-shape distributed stress concentration more evenly than box-type model with sharp line angle, therefore decreased possibility of fracture. 5. Adding grooves to line angles had no effect of decreasing stress concentration to the area.

• Journal of Korean Tunnelling and Underground Space Association
• /
• v.19 no.3
• /
• pp.355-373
• /
• 2017

Tunnelling in urban areas, it is essential to understand existing structure-tunnel interactive behavior. Serviced structures in the city are supported by pile foundation, since they are certainly effected due to tunnelling. In this research, thus, pile load distribution and ground behavior due to tunnelling below grouped pile were investigated using laboratory model test. Grouped pile foundations were considered as 2, 3 row pile and offsets (between pile tip and tunnel crown: 0.5D, 1.0D and 1.5D for generalization to tunnel diameter, D means tunnel diameter). Soil in the tank for laboratory model test was formed by loose sand (relative density: Dr = 30%) and strain gauges were attached to the pile inner shaft to estimate distribution of axial force. Also, settlements of grouped pile and adjacent ground surface depending on the offsets were measured by LVDT and dial gauge, respectively. Tunnelling-induced deformation of underground was measured by close range photogrammetric technique. Numerical analysis was conducted to analyze and compare with results from laboratory model test and close range photogrammetry. For expression of tunnel excavation, the concept of volume loss was applied in this study, it was 1.5%. As a result from this study, far offset, the smaller reduction of pile axial load and was appeared trend of settlement was similar among them. Particulary, ratio of pile load and settlement reduction were larger when the offset is from 0.5D to 1.0D than from 1.0D to 1.5D.

• Journal of Animal Science and Technology
• /
• v.59 no.11
• /
• pp.27.1-27.7
• /
• 2017

Background: Cashew nut shell liquid (CNSL) is an agricultural byproduct containing alkylphenols that has been shown to favorably change the rumen fermentation pattern only under experimentally fixed feeding conditions. Investigation of CNSL potency in rumen modulation under a variety of feeding regimens, and evidence leading to the understanding of CNSL action are obviously necessary for further CNSL applications. The objective of this study was to evaluate the potency of CNSL for rumen modulation under different dietary conditions, and to visually demonstrate its surfactant action against selected rumen bacteria. Methods: Batch culture studies were carried out using various diets with 5 different forage to concentrate (F:C) ratios (9:1, 7:3, 5:5. 3:7 and 1:9). Strained rumen fluid was diluted with a buffer and incubated with each diet. Gas and short chain fatty acid (SCFA) profiles were characterized after 18 h incubation at $39^{\circ}C$. Monensin was also evaluated as a reference additive under the same conditions. Four species of rumen bacteria were grown in pure culture and exposed to CNSL to determine their morphological sensitivity to the surfactant action of CNSL. Results: CNSL supplementation decreased total gas production in diets with 5:5 and 3:7 F:C ratios, whereas the F:C ratio alone did not affect any gas production. Methane decrease by CNSL addition was more apparent in diets with 5:5, 3:7, and 1:9 F:C ratios. An interactive effect of CNSL and the F:C ratio was also observed for methane production. CNSL supplementation enhanced propionate production, while total SCFA production was not affected. Monensin decreased methane production but only in a diet with a 1:9 F:C ratio with increased propionate. Studies of pure cultures indicated that CNSL damaged the cell surface of hydrogen- and formate-producing bacteria, but did not change that of propionate-producing bacteria. Conclusion: CNSL can selectively inhibit rumen bacteria through its surfactant action to lead fermentation toward less methane and more propionate production. As CNSL is effective over a wider range of dietary conditions for such modulation of rumen fermentation in comparison with monensin, this new additive candidate might be applied to ruminant animals for various production purposes and at various stages.

• Asian Pacific Journal of Cancer Prevention
• /
• v.17 no.sup3
• /
• pp.275-278
• /
• 2016

The membrane epithelial mucin MUC1 is expressed at the luminal surface of most simple epithelial cells, but expression is greatly increased in most breast cancers. The aims of present study were to investigate expression of the MUC1 gene and interactive affects in metastases. Whole cell RNA isolation from 50 sentinel lymph nodes (SNLs) of breast cancer patients was performed using reverse transcription and real-time PCR. All patients were diagnosed with breast cancer and without metastasis, confirmed by IHC staining. The evaluation of tumor and normal samples for expression of MUC1 gene, the results were 49.1% non-expressive and 45.3% expression (Student t, p = 0.03). Also in comparison of normal breast tissue and breast cancer SLN for MUC1 gene, MUC1 negative SLNs were 75.0% (18 samples) and MUC1 positive samples were 25.0% (6 samples). Over-expression of MUC1 gene may offer a target for therapy related to progression and metastasis in women with breast cancer.

• Journal of the Korean GEO-environmental Society
• /
• v.21 no.1
• /
• pp.5-12
• /
• 2020

Vibration propagated from seismic sources has damping according to distance and amplification and reduction characteristic in different regions according to topography and geological structure. The vibration propagated from the seismic source to the bedrock is largely affected by the damping according to the separation distance, which can be simply estimated through the damping equation. However, it is important to grasp geological information by location because vibration estimation transmitted to the surface are affected by the natural period of the soil located above the bedrock. Geotechnical investigation data are needed to estimate the seismic intensity based on geological information. If there is no Vs profile, the standard penetration tests are mainly used to determine the soil parameters. The Integrated DB Center of National Geotechnical Information manages the geotechnical survey data performed on the domestic ground, and there is the standard penetration test information of 400,000 holes. In this study, the possibility of quantitation the amplification coefficient for each region was examined to calculated the physical interactive seismic intensity based on geotechnical information. At this time, the shear wave column diagram was generated from the SPT-N value and ground response analysis was performed in the target area. The site coefficients for each zone and the seismic intensity distribution for the seismic motion present a significant difference according to the analysis method and the regional setting.

• Korean Journal of Remote Sensing
• /
• v.13 no.1
• /
• pp.1-12
• /
• 1997

The western equatorial Pacific Ocean, where sea surface temperature is the warmest on the globe, is characterized by numerous convective systems and large annual precipitation. In this region, the cloudiness data with tops higher than 8km level obtained from the GMS-IR data are used to investigate the diurnal variation of cloudiness. The amplitude and phase of diurnal and semi-diurnal cycles are mainly investigated to examine details on the temporal and spatial structure of clouds. Cloudiness variation has typical cycles and each cycle is associated with the air-sea interactive phenomena. Spectral analysis on the cloudiness time series data indicates that 30-60 day, 17-20day, 7-8 day, diurnal and semi diurnal cycle are peaked. During Northern Winter and Southern Summer, the large cloudiness exsists over New Guinea, the adjacent seas of North Australia, and the open oceanic regions east of $160^{\circ}$E. Cloudiness diurnal variability over the lands and their adjacent seas is about 2.0 times larger than that over the open sea regions. That may be due to the difference of specific heat between the land and sea. The maximum and minimum cloudiness appeared at 18:00 and 09:00 hours over the land, and at noon and 21:00 hours over the sea, respectively. The amplitude of diurnal component over the land is 4,7 times larger than that of semi-diurnal component, and 1.5 times over the sea.

• Korean Journal of Food Science and Technology
• /
• v.35 no.4
• /
• pp.604-609
• /
• 2003

In order to investigate the transfer rate of calcium into rice and the effect of soaking conditions-temperature (20, 30, 40, 50 and $60^{\circ}C$, $X_1$), calcium concentration of soaking solution (0, 250, 500, 750 and 1000 mg/L, $X_2$) and soaking time (5, 10, 15, 20 and 25 min, $X_3$), moisture absorption rate, hardness and calcium content of rice after soaking were analyzed. When the soaking temperature and time were increased the moisture absorption rate also increased (p<0.01). Quadratic and interaction effects on the moisture absorption rate among variables were highly significant, within 5%. The predictive model for moisture absorption rate $(Y_1)$ was as follows: $Y=16.14+0.78X_1+1.24X_3-0.23X_1{^2}+0.14X_2{^2}-0.42X_3X_1-0.38X_3{^2}\;(R^2=0.9123)$. Hardness was decreased as soaking time and temperature increased. Calcium contents of soaked rice increased as temperature, the calcium concentration of soaking solution and soaking time were increased (p<0.01). Also the interactive effect between soaking time and calcium concentration of soaking solution was high. The predictive model for calcium content $(Y_3)$ was as follows: $Y_3=470.80+110.87X_1+123.47X_2+52.29X_3-7.42X_1{^2}-14.87X_2{^2}-9.49X_3X_2-20.57X_3{^2}\;(R^2=0.9840)$. From the results in this study, if adults and children take 400 g of calcium enriched rice with 600 mg/kg and $700{\sim}900\;mg/kg$, respectively, they can expect to take more calcium than the recommended intake without other calcium reinforcing agents.