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http://dx.doi.org/10.7314/APJCP.2016.17.S3.275

Overexpression of the MUC1 Gene in Iranian Women with Breast Cancer Micrometastasis  

Mansouri, Neda (Department of Medical Genetics, Shahid Beheshti University of Medical Sciences)
Movafagh, Abolfazl (Department of Medical Genetics, Shahid Beheshti University of Medical Sciences)
Soleimani, Shahrzad (Department of Medical Genetics, Shahid Beheshti University of Medical Sciences)
Taheri, Mohammad (Department of Medical Genetics, Shahid Beheshti University of Medical Sciences)
Hashemi, Mehrdad (Department of Genetics, Tehran medical sciences branch, Islamic Azad University)
Pour, Atefeh Heidary (Department of Cell and Molecular Biology, Pharmaceutical Sciences Branch, Islamic Azad University)
Shargh, Shohreh Alizadeh (Medical Laboratory Science Department, Midwifery-Nursing Institute, Islamic Azad University of Chalous Branch)
Mosavi-Jarahi, Alireza (Department of Epidemiology, School of Medicine, Shahid Beheshti University of Medical Sciences)
Sasaninejad, Zahra (Department of genetic, Islamic Azad University)
Zham, Hanieh (Cancer Research Center, Shohadae Tajrish Hospital, Department of Radiation Oncology, Shahid Beheshti University of Medical Sciences)
Hajian, Parastoo (Cancer Research Center, Shohadae Tajrish Hospital, Department of Radiation Oncology, Shahid Beheshti University of Medical Sciences)
Moradi, Hossein Allah (Department of General Medicen, Shahid Beheshti University of Medical Sciences)
Mirzaei, Hamid Reza (Cancer Research Center, Shohadae Tajrish Hospital, Department of Radiation Oncology, Shahid Beheshti University of Medical Sciences)
Fardmanesh, Hedieh (Molecular Medicine Research Center, Hormozgan University of Medical Sciences)
Ohadi, Mina (Iranian Research Center on Aging, University of Social Welfare and Rehabilitation Sciences)
Publication Information
Asian Pacific Journal of Cancer Prevention / v.17, no.sup3, 2016 , pp. 275-278 More about this Journal
Abstract
The membrane epithelial mucin MUC1 is expressed at the luminal surface of most simple epithelial cells, but expression is greatly increased in most breast cancers. The aims of present study were to investigate expression of the MUC1 gene and interactive affects in metastases. Whole cell RNA isolation from 50 sentinel lymph nodes (SNLs) of breast cancer patients was performed using reverse transcription and real-time PCR. All patients were diagnosed with breast cancer and without metastasis, confirmed by IHC staining. The evaluation of tumor and normal samples for expression of MUC1 gene, the results were 49.1% non-expressive and 45.3% expression (Student t, p = 0.03). Also in comparison of normal breast tissue and breast cancer SLN for MUC1 gene, MUC1 negative SLNs were 75.0% (18 samples) and MUC1 positive samples were 25.0% (6 samples). Over-expression of MUC1 gene may offer a target for therapy related to progression and metastasis in women with breast cancer.
Keywords
Breast cancer; micrometastasis; sentinel lymph node; real-time PC;
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Times Cited By KSCI : 2  (Citation Analysis)
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1 Bernier AJ, Zhang J, Lillehoj E, et al (2011). Non-cysteine linked MUC1 cytoplasmic dimers are required for Src recruitment and ICAM-1 binding induced cell invasion. Mol Cancer, 10, 93.   DOI
2 Besmer DM, Curry JM, Roy LD, et al (2011). Pancreatic ductal adenocarcinoma mice lacking mucin 1 have a profound defect in tumor growth and metastasis. Cancer Res, 71, 4432-42.   DOI
3 Cascio S, Zhang L, Finn OJ (2011). MUC1 protein expression in tumor cells regulates transcription of proinflammatory cytokines by forming a complex with nuclear factor-kappaB p65 and binding to cytokine promoters. J Biol Chem, 286, 42248-56.   DOI
4 Colditz GA, Bohlke K, Berkey CS (2014). Breast cancer risk accumulation starts early: prevention must also. Breast Cancer Res Treat, 145, 567-79   DOI
5 Fardmanesh H, Shekari M, Movafagh A, et al (2016). Upregulation of the double-stranded RNA binding protein DGCR8 in invasive ductal breast carcinoma. Gene, 58, 146-51.
6 Fontenot JD, Mariappan SV, Catasti P, et al (1995). Structure of a tumor associated antigen containing a tandemly repeated immunodominant epitope. J Biomol Struct Dyn, 13, 245-60.   DOI
7 Heidari MH, Porghasem M, Mirzaei N, et al (2014). The effect of high level natural ionizing radiation on expression of PSA, CA19-9 and CEA tumor markers in blood serum of inhabitants of Ramsar, Iran. J Environ Radioact, 128, 64-7.   DOI
8 Hollingsworth MA, Swanson BJ (2004). Mucins in cancer: protection and control of the cell surface. Nat Rev Cancer, 4, 45-60.   DOI
9 Kamata M, Denda-Nagai K, Kubota N, et al (2002). Vaccination of mice with MUC1 cDNA suppresses the development of lung metastases. Clin Exp Metastasis, 19, 689-96.   DOI
10 Kimura T, Tanaka S, Haruma K, et al (2000). Clinical significance of MUC1 and E-cadherin expression, cellular proliferation, and angiogenesis at the deepest invasive portion of colorectal cancer. Int J Oncol,16, 55-64.
11 Ma Y, Ye F, Xie X, et al (2010). Significance of PTPRZ1 and CIN85 expression in cervical carcinoma. Arch Gynecol Obstet, 284, 699-704.
12 Movafagh A, Hajifathali A, Isfahani F, et al (2012). Geographic heterogeneity of cytogenetic characteristics of acute myeloid leukemia in the early detection. Iran J Cancer Prev, 2, 85-9.
13 Movafagh A, Mirfakhraei R, Mousavi-Jarrahi A (2011). Frequent incidence of double minute chromosomes in cancers, with special up-to-date reference to leukemia. Asian Pac J Cancer Prev, 12, 3453-6.
14 Movafagh A, Hajifathali A, Zamani M (2011). Secondary chromosomal abnormalities of de novo acute myeloid leukemia-a first report from the Middle East. Asian Pac J Cancer Prev, 12, 2991-4.
15 Nishidate T, Katagiri T, Lin ML, et al (2004). Genome-wide gene-expression profiles of breast-cancer cells purified with laser microbeam microdissection: identificationof genes associated with progression and metastasis. Int J Oncol, 25, 797-819.
16 Ouellette RJ, Richard D, Maicas E (2004). RT-PCR for mammaglobin genes, MGB1 and MGB2, identifies breast cancer micrometastases in sentinel lymph nodes. Am J Clin Pathol, 121, 637-43.   DOI
17 Pegoraro S, Ros G, Piazza S, et al (2013). HMGA1 promotes metastatic processes in basal-like breast cancer regulating EMT and stem ness. Oncotarget, 4, 1293-308.   DOI
18 Roy LD, Sahraei M, Subramani DB, et al (2010). MUC1 enhances invasiveness of pancreatic cancer cells by inducing epithelial to mesenchymal transition. Oncogene, 30,1449-59.
19 Saintigny P, Coulon S, Kambouchner M, et al (2005). Real-time RT-PCR detection of CK19, CK7 and MUC1 mRNA for diagnosis of lymph node micrometastases in non small cell lung carcinoma. Int J Cancer, 115, 777-82.   DOI
20 Schroeder JA, Adriance MC, Thompson MC, et al (2003). MUC1 alters beta-catenin-dependent tumor formation and promotes cellular invasion. Oncogene, 22, 1324-32.   DOI
21 Seifi-Alan M, Shamsi R, Ghafouri-Fard S, et al (2014). Expression analysis of two cancer-testis genes, FBXO39 and TDRD4, in breast cancer tissues and cell lines. Asian Pac J Cancer Prev, 14, 6625-9.
22 Shargh SA, Sakizli M, Khalaj V, et al. (2014) Downregulation of E-cadherin expression in breast cancer by promoter hypermethylation and its relation with progression and prognosis of tumor. Med Oncol, 31, 250.   DOI
23 Suzuki H, Shoda J, Kawamoto T, et al (2004). Expression of MUC1 recognized by monoclonal antibody MY.1E12 is a useful biomarker for tumor aggressiveness of advanced colon carcinoma. Clin Exp Metastasis, 21, 321-9.   DOI
24 Taylor-Papadimitriou J, Burchell JM, Plunkett T, et al (2002). MUC1 and the immunobiology of cancer. J Mammary Gland Biol Neoplasia, 7, 209-21.   DOI
25 Tinder TL, Subramani DB, Basu GD, et al (2008). MUC1 enhances tumor progression and contributes toward immunosuppression in a mouse model of spontaneous pancreatic adenocarcinoma. J Immunol, 181, 3116-25.   DOI
26 Wakasaki T, Masuda M, Niiro H, et al (2010). A critical role of c-Cbl-interacting protein of 85 kDa in the development and progression of head and neck squamous cell carcinomas through the ras-ERK pathway. Neoplasia, 12, 789-96.   DOI
27 Wang X, Zachary S, Vierkant RA, et al (2010). Association of genetic variation in mitotic kinases with breast cancer risk. Breast Cancer Res Treat, 119, 453-62.   DOI
28 Wei X, Xu H, Kufe D (2006). MUC1 oncoprotein stabilizes and activates estrogen receptor alpha. Mol Cell, 20, 295-305.