• Title/Summary/Keyword: Interaction Effects

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Effect of Energy Sources (Glucose, Pyruvate and Lactate) Added to Dulbecco's Modified Eagle Medium (DMEM) on the Mouse 2-cell Embryo Development (Glutamine 함유 배양액에 첨가한 에너지원이 마우스의 배 발달에 미치는 영향)

  • Kim, Ju-Hwan;Park, Kee-Sang;Lee, Taek-Hoo;Chun, Sang-Sik;Song, Hai-Bum
    • Clinical and Experimental Reproductive Medicine
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    • v.27 no.1
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    • pp.1-7
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    • 2000
  • Objective: Mammalian embryos undergo changes of energy environment for transfer from oviduct to uterus. Also, the human reproductive organ (oviduct, uterus) contains energy sources of different concentration (oviduct - glucose: 0.5 mM, pyruvate: 0.32 mM, lactate: 10.5 mM; uterus - goucose: 3.15 mM, pyruvate: 0.1mM, lactate: 5.87 mM, respectively). This study was conducted to examine the effect of these energy sources added in DMEM with glutamine on the mouse embryo development. Methods: There was used ICR female mouse. Two cell embryos of mouse are collected by method of 'flushing'. Flushing fluid was used Ham's F-10 added to 20% FBS. The collected 2 cell embryos were cultured in media such as Control (only DMEM), group A and B (DMEM supplemented with 0.5 mM and 3.15 mM glucose), and group C and D (DMEM supplemented with 0.1 mM and 0.32 mM pyruvate), and group E and F (DMEM supplemented with 5.87 mM and 10.5 mM lactate). All experimental media supplemented with 20% hFF, respectively. Pattern of embryo development was observed to interval at 24hr during 96hr. Results : The media with glutamine added glucose (group A: 51.0%; group B: 48.4%) was significantly (p<0.05) higher than other experimental group in development into the morula stage after 24 hr in culture, but not significantly different compared with control and the rate of development into the blastocyst was significantly (p<0.05) low in the both of pyruvate (group C: 7.9% group D: 6.8%) and lactate (group E: 7.1%, group F: 7.1%) treatment group after 48 hr in culture. Development into the blastocyst and hatched balstocyst after 72 hr in culture revealed similarly in control (81.9%) and glucose treatment group (group A: 83.3%, group B: 82.8%). However, development into the hatched and attached blastocyst after 96hr in culture revealed significantly (p<0.05) development in the glucose treatment group (group A: 82.3%, group B: 78.5%) than control (63.2%), and its of pyruvate (group C: 34.1%, group D: 34.1%) and lactate (group E: 25.9%, group F: 33.3%) treatment group were significantly (p<0.05) lower than control similar to previous observations. Conclusion : The glucose added to the DMEM with only glutamine, as energy source, was highly to the rate of development compared with control, but the other energy sources were not, synthetically. Above refer to, the human reproductive organ (oviduct, uterus) contains energy sources of different concentration. Thus, further studies are will examine continuously to effects by interaction of different energy sources in the mouse embryo development, and these results will provide to foundation on the human embryo culture.

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A Study of the Effects of Health Contracting on Compliance with Health Behaviors in Clients with Hypertension (자가간호증진을 위한 건강계약이 고혈압자의 건강행위 이행에 미치는 영향)

  • 이향련
    • Journal of Korean Academy of Nursing
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    • v.17 no.3
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    • pp.204-217
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    • 1987
  • It is generally accepted that the delivery of health care is undergoing many changes specially those related to acute, contagious disease care and to the increase of chronic illnesses which can not be cured but are controlable. The health care practitioner can not be soley responsible for the control of their clients' care. Because the clients will play a vital role in controlling their illnesses, long term participation by both the health care provider and the client is necessary. Since most individuals with hypertension do not experience signs or symptoms, the disease is difficult to detect and even when diagnosed, clients do not comply well with their hypertension regimens. The noncompliant client is at increased risk for compliants involving the heart, brain, kidney and other organs. In an effort to explore methods of increasing patient participation in and adherence to treatment programs for hypertension, the researcher used health contracting to promote self care. The research questions are; 1) Will the health contracting increase compliance in health behavior and reduce the blood pressure\ulcorner 2) If clients comply with their regimens will this reduce their blood pressure\ulcorner The research design utilized in this study was a quasi-experimental design. A purposive sample, was abtained from two churches in the 1. area, consisting of 64 clients with hypertension. The data was collected from the middle of January to the 1st of September 1985. Randomization was only of the two church groups into experimental and control groups. Compliance with health behavior related to the hypertensive regimen, blood pressure and body weight were measured, compared and analyzed. In the experimental group measurements were made 6 times; one month before the education program after education program when health contracting was done and 4 more times once a month for 4 months. In the control group measurements were made 3 times; one month before the education program after the education program, and once 4 months later. There was no health contracting. The data were analyzed by t-test, Pearson correlation and ANOVA according to purpose of the study. The result of this study may be summarized as follows: The result related to the hypothesis on the effect of health contracting are as follows: H$_1$; “The hypothesis that the experimental group, with a health contractual agreement will demonstrate increased compliance levels for health behavior than the control group” was supported(t=-5.29, df=62, p=.000). H$_2$; “The hypothesis that the experimental group, with a health contractual agreement, will demonstrate a greater reduction in blood pressure than the control group” was supported (for systolic blood pressure t=2.72, df=62, p=.009, for diastolic blood pressure t=1.95, df=62, p=.050). H$_3$; The hypothesis that the greater the compliance of the client with health behavior the lower the client's blood pressure will be was partially supported (for systolic pressure r=-.2981, p=.008, for diastolic pressure r=-.1720, p=.087). From the examination of the results of this study it can be concluded that the interaction between the nurse and the client, contracting to define goals and reinforcing compliant behavior, leads to improved compliance with health care behaviors and thus to an increase in the effectiveness of nursing care. Further consideration need to be given to the inclusion of the concept of health contracting in primary nursing and to further research in this area.

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The Effect of the Satisfaction after Consumption and Consumer Self-Confidence for Hedonic Products on Transaction Coupling (소비 후 만족도와 소비자 자신감이 거래 커플링에 미치는 영향 - 쾌락적 제품을 중심으로 -)

  • Kang, Seong-Min;Kang, Hyun-Mo
    • CRM연구
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    • v.4 no.2
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    • pp.1-17
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    • 2011
  • In the study of transaction coupling and consumer behavior it is argued that the satisfaction after consumption and consumer self-confidence would affect the degree of transaction coupling. Based on Kivetz(1999), this study expand transaction coupling which is a mental accounting process. Satisfaction after consumption and consumer self-confidence have been frequently cited as a key construct for predicting various consumer-related behaviors. The purpose of this research is to examine the effect of satisfaction after consumption and consumer self-confidence for hedonic products on transaction coupling. In order to explain the impact of consumer self-confidence clearly, the authors used a five-factor(i.e., information acquisition, consideration-set formation, personal and social outcomes, persuasion knowledge and marketplace interfaces). Using the scenario about baseball game, the authors manipulated the consumer satisfaction after consumption (satisfaction vs. dissatisfaction) between-subjects design. And consumer self-confidence was measured based on Bearden et al.(2001). The results of experimental study showed that the main effects of satisfaction after consumption is significant. The larger consumer satisfaction after consumption reflected a higher degree of transaction coupling. The 2-way interaction between satisfaction after consumption and consumer self-confidence is also significant. Specifically, the transaction coupling differentiation from satisfaction after consumption tends to be larger at high consumer-self confidence than at low one.

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Effect of Experiential Space Perception on Performing Interactive Digital Contents (체험적 공간감이 상호작용 콘텐츠 수행에 미치는 영향)

  • Yun, Han-Kyung;Song, Bok-Hee
    • The Journal of Korea Institute of Information, Electronics, and Communication Technology
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    • v.4 no.2
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    • pp.111-118
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    • 2011
  • It is not easy to define a boundary between TV and home computer in these day since developing H/W and S/W of computer technology induces that TV is conflated with computer. Coming digital HD broadcasting forces for replacement of TV at home and the trend of TV is became bigger. The evolved TV is able to replace the computer by connecting to the network and people want to do interactions with contents by using the bidirectional communication. Therefore, it is expected to changing the human lifestyle. It is natural that contents for all members of family are needed, since screen of TV become bigger. It is required that the contents should guarantees the accessability of information to the all of family members and the easy interaction with contents. But, the related basic research is not enough to catch the user's eye to induce flow or presence. Our goal of this study is to analyse effects of experiential space perception on performing interactive digital contents. The result shows that users interacted with contents without any difficulty when they met a same dimension and shape of objects as dimension and shape objects in their experiences or learning.

The Effect of Emotional Coaching Parent Education Program on Parenting Efficacy, Parenting Attitude, and Emotional Expressiveness (감정코칭 부모교육 프로그램이 양육효능감 및 양육행동, 정서표현성에 미치는 효과)

  • Beak, Seung Seon;Ahn, Youn Kyung;Kwak, Kyeong Hwa
    • Korean Journal of Child Education & Care
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    • v.17 no.2
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    • pp.1-24
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    • 2017
  • The purpose of this study is to present information about effective parent education method by examining effects of parenting education program on emotional coaching, parenting efficacy, parenting attitude and emotional expression. For this purpose, 43 parents who have a toddler who is financed in H daycare center and Y daycare center in G city of Jeonbuk were selected as research subjects. Among them, twenty - two mothers of infants who are financially supported by H day care center were selected as experiment group and they conducted a total of 8 cycles of emotional coaching parent education program for 120 minutes per week and once a week. For the analysis of collected data, ANCOVA was performed using SPSS 18.0 program. The results were as follows. The parenting program of emotional coaching positively affects mother 's parenting efficacy, parenting attitude and emotional expressiveness. The results of this study can be used as basic data of parent education method which helps the desirable interaction with infant through more specific steps through emotional coaching to parents with toddlers.

The Longitudinal Relationship among Paternal Involvement, Maternal Parenting Stress, Psychosocial Development of Infant during Infancy and Peer Interactions during Childhood (영아기 아버지 양육참여, 어머니 양육스트레스, 영아 심리사회발달과 유아기 또래상호작용 간의 종단적 관계 분석)

  • Jang, Hyo Eun;Kim, Choon Kyung
    • Korean Journal of Child Education & Care
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    • v.18 no.1
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    • pp.77-102
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    • 2018
  • The objectives of this study were to analyze the longitudinal mediation effects of maternal parenting stress and psychosocial development of infant on the influence of paternal involvement during infancy to children's peer interactions. For these objectives, latent growth modeling was used and adapted to the data taken from the Panel Study on Korean Children(PSKC), Wave I (0-year olds) to Wave III (2-year olds) and Wave V(4-year olds) to Wave VII(6-year olds). During a total of six years, 1,018 parent responded. The major results of this study were as follows. First, maternal parenting stress(initial status) had a significant mediating effect on the influence of paternal involvement(initial status) to children's play interaction(initial status). Second, maternal parenting stress(initial status/change rate) had a longitudinal mediating eff ect on the influence of paternal involvements(initial status/change rate) to children's play disruption-disconnection interactions(initial status/change rate). Third, psychosocial development of infants(initial status/change rate) had a longitudinal mediating effect on the influence of paternal involvement(initial status/change rate) to children's play interactions(initial status/change rate). Fourth, psychosocial development of infant(initial status) had a significant mediating effect on the influence of paternal involvement(initial status) to children's play disruption-disconnection interactions(initial status). The implications of the findings of the current study and suggestions for future research were also discussed.

Impacts of wave and tidal forcing on 3D nearshore processes on natural beaches. Part I: Flow and turbulence fields

  • Bakhtyar, R.;Dastgheib, A.;Roelvink, D.;Barry, D.A.
    • Ocean Systems Engineering
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    • v.6 no.1
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    • pp.23-60
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    • 2016
  • The major objective of this study was to develop further understanding of 3D nearshore hydrodynamics under a variety of wave and tidal forcing conditions. The main tool used was a comprehensive 3D numerical model - combining the flow module of Delft3D with the WAVE solver of XBeach - of nearshore hydro- and morphodynamics that can simulate flow, sediment transport, and morphological evolution. Surf-swash zone hydrodynamics were modeled using the 3D Navier-Stokes equations, combined with various turbulence models (${\kappa}-{\varepsilon}$, ${\kappa}-L$, ATM and H-LES). Sediment transport and resulting foreshore profile changes were approximated using different sediment transport relations that consider both bed- and suspended-load transport of non-cohesive sediments. The numerical set-up was tested against field data, with good agreement found. Different numerical experiments under a range of bed characteristics and incident wave and tidal conditions were run to test the model's capability to reproduce 3D flow, wave propagation, sediment transport and morphodynamics in the nearshore at the field scale. The results were interpreted according to existing understanding of surf and swash zone processes. Our numerical experiments confirm that the angle between the crest line of the approaching wave and the shoreline defines the direction and strength of the longshore current, while the longshore current velocity varies across the nearshore zone. The model simulates the undertow, hydraulic cell and rip-current patterns generated by radiation stresses and longshore variability in wave heights. Numerical results show that a non-uniform seabed is crucial for generation of rip currents in the nearshore (when bed slope is uniform, rips are not generated). Increasing the wave height increases the peaks of eddy viscosity and TKE (turbulent kinetic energy), while increasing the tidal amplitude reduces these peaks. Wave and tide interaction has most striking effects on the foreshore profile with the formation of the intertidal bar. High values of eddy viscosity, TKE and wave set-up are spread offshore for coarser grain sizes. Beach profile steepness modifies the nearshore circulation pattern, significantly enhancing the vertical component of the flow. The local recirculation within the longshore current in the inshore region causes a transient offshore shift and strengthening of the longshore current. Overall, the analysis shows that, with reasonable hypotheses, it is possible to simulate the nearshore hydrodynamics subjected to oceanic forcing, consistent with existing understanding of this area. Part II of this work presents 3D nearshore morphodynamics induced by the tides and waves.

Comparison Study of the Impact Response Characteristics of Fixed Cylindrical Offshore Structures Considering Seawater Fluid Region (해수유체영역을 고려한 고정식 실린더형 해양구조물의 충격응답특성 비교연구)

  • Lee, Kangsu;Hong, Keyyong
    • Journal of Advanced Marine Engineering and Technology
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    • v.39 no.4
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    • pp.489-494
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    • 2015
  • This research focused on minimizing the response of fixed cylindrical offshore structures to a ship impact considering the seawater fluid part. A collision between a ship and offshore structure is generally a complex problem and it is often impractical to perform rigorous finite element analyses to include all the effects and sequences during the collision. The structural behavior of a fixed cylindrical type offshore substructure with a seawater fluid part has a simpler response and small deformation due to the dissipation of impact energy. Upon applying the impact force of a ship to the cylindrical structure, the maximum acceleration, internal energy, and plastic strain are calculated for each load cases using Ls-dyna finite element software. In the maximum cases 2.0 m/s velocity, the response result for the structure was carried out to compare between having a fluid region and no fluid region. Fluid-structure interaction analysis was performed using the ALE method, which make it possible to apply a fluid region on the impact problem. The case of a fixed cylindrical type offshore structure without a seawater fluid part can be a more conservative design.

Effect of Light Intensity and Temperature on the Photosynthesis and Respiration of Panax spp (광도와 온도가 인삼의 광합성 및 호흡에 미치는 영향)

  • 이종화
    • Journal of Ginseng Research
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    • v.12 no.1
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    • pp.11-29
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    • 1988
  • This study was conducted to investigate the effect of light intensity and temperature on the photosynthesis and respiration of ginseng plant. Highly significant, second degree curvilinear regressions were recognized among the photosynthesis of ginseng leaves, light intensity and temperature. And an interaction between the effects of light intensity and temperature on the photosynthesis of ginseng leaves was found to be highly significant. The increasing rate of photosynthesis with the increase of light intensity was markedly decreased with increasing temperature. The light compensation point of ginseng leaves was significantly varied with temperature, and the average point was approximately 600 lux. The light saturation point of Korean ginseng was 11,000 lux at $15^{\circ}C$ and $20^{\circ}C$ and around 9,500 lux at above $25^{\circ}C$. The decreasing rate of photosynthesis with the increase of temperature significantly increased with increasing light intensity. The optimum temperature for the photosynthesis of ginseng leaves was about 15 to $22^{\circ}C$ and markedly decreased with increasing light intensity. The highest photosynthesis occurred in ginseng leaves grown with the shade of 15% transmittance. The respiration of ginseng leaves increased with the shade of 5% and/or 30% transmittance. High temperature stimulated the respiration of ginseng leaves. Percent respiration to photosynthesis of ginseng leaves grown with the shade was increased at high temperature and decreased with increasing light Intensity. It was also increased with increasing transmittance. The maximum $CO_2$ absorption of ginseng leaves grown with the shade of 5Ps and ISVS transmittance accurred at 9 o'clock a.m., whereas that of 20% transmittance occurred at 7-9 o'clock a.m. The duration of $CO_2$ absorption was distinctively long with the shade of high transmittance. The $CO_2$ compensation point in the photosynthesis of ginseng leaves was 130 ppm.

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Mechanism of $Ca^{2+}$ -activated $Cl^-$ Channel Activation by Ginsenosides in Xenopus Oocytes

  • Park, Seok;Jung, Se-Yeon;Park, Seong-Hwan;Ko, Sung-Ryong;Hyewon Rhim;Park, Chul-Seung;Nah, Seung-Yeol
    • Journal of Ginseng Research
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    • v.24 no.4
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    • pp.168-175
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    • 2000
  • Relatively little is known about the signaling mechanism of ginseng saponins (ginsenosides), active ingredients of ginseng, in non-neuronal cells. Here, we describe that ginsenosides utilize a common pathway of receptor-mediated signaling pathway in Xenopus oocytes: increase in intracellular $Ca^{2+}$ concentration via phospholipase C (PLC) and $Ca^{2+}$ mobilization. Ginsenosides induced a marked and robust artivation of $Ca^{2+}$-activated Cl- channels in Xenopus oocytes. The effect of ginsenosides was completely reversible, in a dose-dependent manner with EC$_{50}$ of 4.4 $\mu\textrm{g}$/mi, and specifically blocked by niflumic acid, an inhibitor of $Ca^{2+}$-activated Cl- channel. Intracellular injection of BAPIA abolished the effect of ginsenosides. Intracellular injection of GTP${\gamma}$S also abolished the effect of ginsenosides. The effect of gin senosides on $Ca^{2+}$-activated Cl- currents was greatly reduced by the intracellular injection of heparin, an IP$_3$ receptorantagonist or the pretreatment of PLC inhibitor. These results indicate that ginsenosides activate endogenous $Ca^{2+}$-activated Cl- channels via the activation of PLC and the release of $Ca^{2+}$ from the IP$_3$-sensitive intracellular store following the initial interaction with membrane component(s) from extracellular side. This signaling pathway of ginsenosides may be one of the action mechanisms for the pharmacological effects of ginseng.ts of ginseng.

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