• 생명과학회지
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• 제17권11호
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• pp.1482-1487
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• 2007

ISSR분자 마커를 이용하여 왕대속 대나무의 유연관계를 분석 실시하였다. 전세계적으로 왕대속에 속하는 4종은 경제학적, 생태학적으로 중요한 식물 자원중 하나이다. 총 11개의 ISSR 프라이머 중 9개의 프라이머에서 증폭이 일어 났으며 총 64개의 밴드를 확인 할 수 있었다. ISSR분석결과 왕대속 4종의 대나무에서 70.49%인 43개의 다형현상이 나타났다. 4개의 분류군으로 분류된 왕대속 대나무의 집단내 다양성(Hs)은 0.092,집단간 다양성(Gst)은 0.499로 나타났다. 각 종에 대한 유전자 유동(Nm)은 0.559로 나타났다. 따라서 왕대속에 속하는 4종의 유전자 유동(Nm)은 아주 낮음을 알 수 있었다 4종의 분류군에 대한 유전자 다양성(Ht)은 0.291로 낮게 나타났으며 ISSR 마커로 명확하게 그들은 분류가 되었다. 또한 왕대속의 4종은 단일계통임을 확인할 수 있었다.

• The Plant Pathology Journal
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• 제36권1호
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• pp.11-28
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• 2020

Faba bean (Vicia faba L.) is one of the most important legume crops in Egypt. However, production of faba bean is affected by several diseases including fungal diseases. Fusarium wilt incited by Fusarium oxysporum Schlecht. was shown to be the most common wilt disease of faba bean in Assiut Governorate. Evaluation of 16 faba bean genotypes for the resistance to Fusarium wilt was carried out under greenhouse conditions. Three molecular marker systems (inter-simple sequence repeat [ISSR], sequence related amplified polymorphism [SRAP], and simple sequence repeat [SSR]) and a biochemical marker (protein profiles) were used to study the genetic diversity and detect molecular and biochemical markers associated with Fusarium wilt resistance in the tested genotypes. The results showed that certain genotypes of faba bean were resistant to Fusarium wilt, while most of the genotypes were highly susceptible. The percentage of disease severity ranged from 32.83% in Assiut-215 to 64.17% in Misr-3. The genotypes Assiut-215, Roomy-3, Marut-2, and Giza2 were the most resistant, and the genotypes Misr-3, Misr-1, Assiut-143, Giza-40, and Roomy-80 performed as highly susceptible. The genotypes Assiut-215 and Roomy-3 were considered as promising sources of the resistance to Fusarium wilt. SRAP markers showed higher polymorphism (82.53%) compared with SSR (76.85%), ISSR markers (62.24%), and protein profile (31.82%). Specific molecular and biochemical markers associated with Fusarium wilt resistance were identified. The dendrogram based on combined data of molecular and biochemical markers grouped the 16 faba bean genotypes into three clusters. Cluster I included resistant genotypes, cluster II comprised all moderate genotypes and cluster III contained highly susceptible genotypes.

• 원예과학기술지
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• 제29권6호
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• pp.600-609
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• 2011

Allium속에 포함된 6종의 122점을 수집하고, 이 종들의 유전적 관계는 ISSR 마커를 이용하여 확인하였다. 형태적 분석은 6개의 양적 형질을 측정하고 1개의 질적 형질은 수치화하였다. SSR 분석은 17개의 primer를 사용하여 총 370개의 다형성 밴드를 얻었다. 형태적 특성 분석은 유전적 거리로 구분할 경우 3개의 그룹으로 분류되었으나, 부분적으로 몇 몇 종들은 분류에 어려움이 있었다. ISSR 결과를 바탕으로 Allium 속의 군집분석은 5개의 그룹으로 분리되었다. 형태적 분석과 SSR분석 간의 상관 관계는 유의성이 매우 낮았다(r = 0.036). 따라서 본 연구에서 개발한 ISSR 마커는 달래와 산달래의 분류와 교배 육종에 유용하게 이용될 수 있을 것이다.

• 식물분류학회지
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• 제43권3호
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• pp.236-243
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• 2013

북방계 식물들의 잔존적 피난처인 아고산대에 분포하는 식물들은 기후변화에 따른 자생지 환경변화로 인해 멸종 및 멸절 위험에 크게 직면해 있는 실정이다. 본 연구는 한반도의 남부지역 아고산대에 제한적으로 분포하고 있는 특산식물이며 희귀식물인 설앵초(Primula farinosa subsp. modesta (Bisset & Moore) Pax)에 대한 유전적 다양성과 지리적 분화 양상을 구명을 통한 보존전략을 수립하기 위해 3지역 6집단 165개체를 대상으로 ISSR (Inter Simple Sequence Repeat) 분석을 수행하였다. 그 결과 집단 수준의 유전 다양성의 평균은(P = 60.62, SI = 0.299, h = 0.190) 유사한 생활사를 갖는 다년생 초본류의 평균보다 낮은 수준이었으며, 이형화주의 특징을 갖는Primula속의 근연 분류군들에 비해서도 다소 낮은 결과를 보였다. 유전적 분화도 구명을 위해 AMOVA(Analysis of molecular variance) 분석 결과 전체 유전변이 중 약 50%가 지역 간에 분포하는 결과를 보여 종내 지역간 분화도가 매우 높은 수준으로 판단되며, Bayesian cluster 분석 결과에서도 조사된 세 지역이 각각 독특한 유전적 cluster를 형성함으로써 개체군간 유전자 이동이 매우 제한적임을 암시하였다. 따라서 설앵초 집단의 급속한 분획화와 낮은 수준의 유전적 다양성, 지역간 높은 분화도 등의 특성들을 고려하여 설앵초 집단의 개체군 감소 및 멸절 방지를 위한 현지 내 외 보전 전략 수립이 시급한 것으로 판단된다.

• 생명과학회지
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• 제17권1호
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• pp.24-30
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• 2007

꽃치자(Gardenia jasminoides var. radicans)와 열매치자(C. jasminoides for. grandiflora)는 Gardenia속으로는 우리나라에 두 분류군밖에 없으며 열매치자는 약용, 식용, 꽃치자는 방향제로 쓰인다. 그런데 꽃이 지고 나면 형태적으로 구분이 거의 되지 않는다 ISSR분석으로 이들 종의 유전적 다양도와 집단구조를 실시하였다. 88개의 DNA 분절에서 한 분류군에만 나타나는 특이밴드가 탐지되었다. 한국의 세 야생 집단은 분리되어 있고 패치 분포를 보이지만 재배종에 비해 높은 유전적 다양성을 유지하친 있었다. 열매치자가 꽃치자보다 유전적 다양성이 높았으며 ISSR 마커로 이들 분류군이 잘 분리되었다. 또한 야생 집단이 재배 집단보다 다양성이 약간 높으나 유의성은 없었다. 이는 재배화과정에서 유전적 다양성의 일부 상실이 있었으나 인위적인 채취와 식재로 야생 집단과 재배 집단의 유전적 교류가 존재하였음을 시사한다.

• International Journal of Industrial Entomology and Biomaterials
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• 제19권2호
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• pp.249-253
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• 2009

Raily ecorace of Indian tasar silkworm is wild in nature and distributed abundantly in dense deciduous forest on Shorea robusta (Sal) in Bastar ($17^{\circ}4'$ and $20^{\circ}34'$ N, $80^{\circ}15'$ and $82^{\circ}15'$ E and altitude ranging from 150 to 1200 mMSL) forest ranges of Chhattisgarh, India. It is represented by about 20 populations. Out of those, eleven populations showed intra- as well as inter- population variability based on phenotypic expression and also in major economic traits viz. cocoon weight, shell weight, filament length and denier. Genetic diversity in these eleven populations was studied using Inter-Simple Sequence Repeat (ISSR) markers. The band profiles generated with eight ISSR primers have depicted variation in band size. All the primers exhibited polymorphism which is an indicative of the genetic variation in individual Raily silkworm. Among the populations, total polymorphism recorded was 76%. The population genetic aspects assessed through POPGENE software package are discussed in the paper. Nei's gene diversity (h) ranged from 0.194 to 0.337 exhibiting high heterozygosity. Relevance of the present study is of high significance in formulating conservation strategies and sustainable utilization of the economically important Raily ecorace of Antheraea mylitta.

• 한국약용작물학회지
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• 제13권5호
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• pp.249-253
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• 2005

This study was carried out to develop convenient and reproducible methods for identifying the genetic relationship among germplasms of Panax species based on molecular genetics. Using random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) analyses, genetic polymorphism of the Panax species was investigated with following cultivars and accessions, such as Chunpoong, Yunpoong, Kopoong, Sunpoong, and Kumpoong in domestic cultivars, Hwangsuk, Jakyung and Suckju in domestic accessions, and Panax quinquefolius L. and Panax japonicus C.A. Meyer in foreign introduced accessions, respectively. Specific DNA fragments ranging from 200 to 3,000 base pairs in size could be obtained with various ISSR and RAPD primers under the optimized PCR conditions. The dissimilarity coefficients among the genetic polymorphisms of ginseng cultivars and accessions were calculated from 0.26 to 0.90 in RAPD and from 0.12 to 0.89 in ISSR analysis, respectively. Eleven plant samples were grouped siblings together with cultivars and parents based on cluster analysis of genetic distance depending on genetic property such as origin of the species. In results, both RAPD and ISSR analyses were useful for identifying the genetic relationship among cultivars and accessions of Panax species at DNA level.

• International Journal of Industrial Entomology and Biomaterials
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• 제30권1호
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• pp.6-16
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• 2015

This study established the genetic characterisation of 10 microsporidian isolates infecting non-mulberry silkworms (Antheraea assamensis and Samia cynthia ricini) collected from biogeographical forest locations in the State of Assam, India, using PCR-based markers assays: inter simple sequence repeat (ISSR) and random amplified polymorphic DNA (RAPD). A Nosema type species (NIK-1s_mys) was used as control for comparison. The shape of mature microsporidian spores were observed oval to elongated, measuring 3.80 to $4.90{\mu}m$ in length and 2.60 to $3.05{\mu}m$ in width. Fourteen ISSR primers generated reproducible profiles and yielded 178 fragments, of which 175 were polymorphic (98%), while 16 RAPD primers generated reproducible profiles with 198 amplified fragments displaying 95% of polymorphism. Estimation of genetic distance coefficients based on dice coefficients method and clustering with un-weighted pair group method using arithmetic average (UPGMA) analysis was done to unravel the genetic diversity of microsporidians infecting Indian muga and eri silkworm. The similarity coefficients varied from 0.385 to 0.941 in ISSR and 0.083 to 0.938 in RAPD data. UPGMA analysis generated dendrograms with two microsporidian groups, which appear to be different from each other. Based on Euclidean distance matrix method, 2-dimensional distribution also revealed considerable variability among different identified microsporidians. Clustering of these microsporidian isolates was in accordance with their host and biogeographic origin. Both techniques represent a useful and efficient tool for taxonomical grouping as well as for phylogenetic classification of different microsporidians in general and genotyping of these pathogens in particular.

• 한국자원식물학회지
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• 제28권6호
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• pp.697-703
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• 2015

Twenty apple germplasm accessions from the Korean Genebank were successfully cryopreserved using two-step freezing to back up genetic resources maintained by field collections. This study examined the morphological and genetic stability of cryopreserved dormant apple buds that were stored in liquid nitrogen, and then rewarmed and regrown. Whole plants were regenerated directly from dormant buds through budding without an intermediary callus phase. The cryopreserved buds produced high levels of shoot formation (76.2-100%), similar to those of noncryopreserved buds (91.3-100%), with no observed differences between cryopreserved and noncryopreserved materials. Three of the twenty cryopreserved apple germplasm accessions were used to assess morphological and genetic stability. No differences in morphological characteristics including shoot length, leaf shape, leaf width/length ratio, and root length were observed between controls (fresh control and noncryopreserved) and cryopreserved plantlets. The genetic stability of regenerants (before and after cryopreservation) was investigated using inter simple sequence repeat (ISSR) markers. The ISSR markers produced 253 bands using four primers, ISSR 810, SSR 835, ISSR 864, and ISSR 899. These markers showed monomorphic banding patterns and revealed no polymorphism between the mother plant and regenerants before and after cryopreservation, suggesting that cryopreservation using two-step freezing does not affect the genetic stability of apple germplasm. These results show that two-step freezing cryopreservation is a practical method for long-term storage of apple germplasms.

• Journal of Forest and Environmental Science
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• 제24권1호
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• pp.27-34
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• 2008

Level and distribution of genetic diversity in seven populations of Albizia lucida Benth. in eastern region of the Indian sub-continent were estimated using ISSR markers. Relatively higher level of genetic diversity within populations was observed in seven populations of A. lucida (mean of 0.38). From the result of AMOVA, majority of genetic diversity was allocated within populations (96.2%) resulting in a moderate degree of population differentiation. The observed distribution pattern of I-SSR variant among the populations was coincided with the typical pattern of long-lived woody tree species. Genetic relationships among the populations, reconstructed by UPGMA method, revealed two genetic groups. The population of Anugul and Bargarh turned out to be the most closely related despite a distance location between them. These formations will be of great value in the development of conservation plans for species exhibiting high levels of genetic differentiation due to fragmentation, such as indication of conservation unit size, which populations should be chosen as priority in conservation plans and which samples should be introduced in areas with a low number of individuals of A. lucida.