• 한국생물정보학회:학술대회논문집
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• 한국생물정보시스템생물학회 2005년도 BIOINFO 2005
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• pp.305-310
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• 2005

Internal motion of the protein has been described in many papers with C$_{\alpha}$ correlation coefficients to find motional correlation and functional characteristics. To describe the secondary structural motion and stability in protein, we have studied molecular dynamics (MD) simulations on FADD Death Domain and FADD Death Effector Domain which have a similar structure but have different functional characteristics. After 10ns MD simulations, the inter-helical motional correlations and the hydrogen bond ratios were compared between the two domains. From these data we could distinctly compare the internal motions of them and could explain the differences in experimental thermodynamic melting behaviors at molecular level.

• 대한전자공학회:학술대회논문집
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• 대한전자공학회 2003년도 하계종합학술대회 논문집 II
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• pp.823-826
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• 2003

FPGA-based logic emulator with lane gate capacity generally comprises a large number of FPGAs connected in mesh or crossbar topology. However, gate utilization of FPGAs and speed of emulation are limited by the number of signal pins among FPGAs and the interconnection architecture of the logic emulator. The time-multiplexing of interconnection wires is required for multi-FPGA system incorporating several state-of-the-art FPGAs. This paper proposes a circuit partitioning algorithm called SCATOMi(SCheduling driven Algorithm for TOMi)for multi-FPGA system incorporating four to eight FPGAs where FPGAs are interconnected through TOMi(Time-multiplexed, Off-chip, Multicasting interconnection). SCATOMi improves the performance of TOMi architecture by limiting the number of inter-FPGA signal transfers on the critical path and considering the scheduling of inter-FPGA signal transfers. The performance of the partitioning result of SCATOMi is 5.5 times faster than traditional partitioning algorithms. Architecture comparison show that the pin count is reduced to 15.2%-81.3% while the critical path delay is reduced to 46.1%-67.6% compared to traditional architectures.

• 한국해양학회지:바다
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• 제4권3호
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• pp.246-253
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• 1999

퇴적물 입도분석 방식에 따른 분석결과의 편차를 비교하였다. 한반도 주변해역에서 기존에 보고된 퇴적물 입도자료들은 연구기관에 따라 상당한 편차가 나타났으며, 실험실간 편차보정작업(Inter-lab calibration)이 요구된다. 퇴적물 입도분석 결과는 분석방식에 따라 상이하게 나타났다. 레이저 회절방식은 반복측정의 정확도가 매우 우수하였지만, 2-3 ${\mu}m$보다 세립한 계층의 함량을 과소측정하였다. 이와 반면에 입자침전속도에 근거한 입도분석 방식은 조립실트(5-6 ${\phi}$) 계층의 오차가 크게 나타났으며, 점토질 함량이 다소 과다하게 측정되었다. 퇴적물 입도분석에 가장 일반적으로 활용되는 체질과 피켓법은 0.5 ${\phi}$ 간격의 분석작업이 가장 효과적인 것으로 나타났다. 특히 피켓법의 경우 조립실트 계층의 영역에서 0.5 ${\phi}$ 간격의 분석작업이 요구되었다. 또한 사질 퇴적물의 체질방식에서는 하나의 체에 걸러지는 퇴적물 양에 따른 오차가 매우 중요한 것으로 나타났다.

• 한국대기환경학회지
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• 제32권3호
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• pp.305-319
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• 2016

This study was carried out to evaluate the performance of two sampling methods, i.e., adsorbent tubes and canisters, for the measurement of ambient volatile organic compounds (VOCs). A total of 24 target VOCs were selected from a list of 48 priority hazardous air pollutants (HAPs) in Korea. The two sampling methods were investigated with a wide range of performance criteria such as repeatability, linearity, and lower detection limits. In addition, mean relative errors (MRE) and mean duplicate precisions (MDP) were estimated by inter-lab comparison studies for duplicate field samples. Precisions for the two methods appeared to be well comparable with the performance criteria recommended by USEPA TO-15 and TO-17 for canister and adsorbent methods, respectively. Correlations and variations between the VOCs concentrations determined by the two methods were generally good in most cases. However, MREs and MDPs for individual VOCs appeared to be widely ranged, depending on each VOC. This implies that the two methods have its own advantages and disadvantages in determining a variety of VOCs in ambient air, and neither of which has absolute superiority. Finally, 9 of 24 VOCs were found to be difficult to determine by either methods due to their unstability in a canister, and lack of appropriate standard materials. Thus, it is suggested that development of measurement methods for such unstable VOCs is an urgent task from a viewpoint of HAPs management.

• 제어로봇시스템학회논문지
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• 제21권6호
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• pp.589-594
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• 2015

In this paper, we described the Cramer-Rao Lower Bound (CLRB) performances of Time Difference of Arrival (TDOA) and Frequency Difference of Arrival (FDOA) methods when there are multiple emitters. The TDOA and FDOA values between two receivers can be simultaneously estimated by using the so-called Complex Ambiguity Function (CAF). In the case of multiple emitters, there exist Inter Symbol Interferences (ISIs) in the measurement data. Therefore, it is required to reduce the effect of ISI and provide a performance evaluation method of TDOA and FDOA estimations. In order to eliminate the ISIs, using of a filter bank before calculating CAF is proposed when the carrier frequencies of the emitters are different to one another. Angle of Arrival (AOA) or Received Signal Strength (RSS) methods before calculating CAF were proposed to reduce the ISIs when the carrier frequencies are the same. In order to evaluate the CRLB of TDOA and FDOA estimations, we employed the conditional probability distribution method and described the numerical comparison results.

• 한국대기환경학회지
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• 제15권2호
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• pp.121-138
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• 1999

This study was carried out to evaluate the performance of a sampling and analytical methodology for the measurement of selected volatile organic compounds (VOCs) in the ambient air. VOCs were determined by the adsorbent tube sampling and automatic thermal desorption coupled with GC/FID and GC/MSD analysis. Target analytes were aromatic VOCs, including BTEX, 1,3,5-and 1,2,4,-trimethylbenzenes(TMBs), and naphthalene. The methodology was investigatedwith a wide range of performance criteria such as repeatability, linearity, lower detection limits, collection efficiency, thermal conditioning, breakthrough volume and calibration methods using internal and external standards. standards. Stability of samples collected on adsorbent tubes during storage was also investigated. In addition, the sampling and analytical method developed during this study was applied to real samples duplicately collected in various indoor and outdoor environments. Precisions for the duplicate samples and distributed volume samples appeared to be well comparable with the performance criteria recommended by USEPA TO-17. The audit accuracy was estimated by inter-lab comparison of both duplicate samples and standard materials between the two independent labs. The overall precision and accuracy of the method were estimated to be within 30% for major aromatic VOCs such as BTEX. This study demonstrated that the adsorbent sampling and thermal desorption method can be reliably applied for the measurement of BTEX in ppb levels frequently occurred in common indoor and ambient environments.

• 생약학회지
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• 제37권4호
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• pp.278-282
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• 2006

In this study, we analyzed and quantified the contents of magnolol (1), honokiol (2), and obovatol (3) in the leaves of Magnolia species by HPLC. HPLC methods were developed and validated by ICH guide lines comparing the linearity, intraday precision, inter-day precision. The optimal conditions were as follows; wavelength: 288 nm, column: Zorbax Eclipse XDBC18 $(2.1\times150\;mm,\;5{\mu}m)$, mobile phase: Acetonitril - 0.1 % $H_3PO_4$ (45:55, v/v) buffer, flow rate: 0.5 ml/min. The contents of magnolol, honokiol and obovatol were 1.08%, 2.37%, and 0.57% in the leaves of M. officinalis, 0.50%, 0.03%, and 1.54% in the leaves of M. biloba, 0.37%, 0.03%, and 0.76% in the leaves of M. obovata. The contents of effective ingredients in the leaves of M. officinalis and M biloba were higher than that of M. obovata. Especially, the content of obovatol in the leaves of M. biloba was remarkably high. This result showed that the shapes of leaves have a certain relationship with the content of obovatol.

• 한국해양생명과학회지
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• 제9권1호
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• pp.9-21
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• 2024

마비성 패류 독소(Paralytic shellfish poisoning, PSP)는 유해 조류에 의해 생성되며, 독소에 노출된 수산물을 섭취하였을 때 중독이 발생한다. 수산물 중 PSP를 검출하는 표준 시험법인 Mouse bioassay (MBA)는 낮은 검출한계와 동물 윤리 문제로 대체 시험법의 개발 필요성이 대두되고 있다. 이러한 대체 시험법 중, PSP가 신경 세포막의 Na⁺ 채널을 차단하는 기전을 이용한 마우스 뇌신경 모세포종 세포 기반 시험법(Neuro-2a assay)의 표준화를 위한 노력이 대두되고 있다. Neuro-2a assay의 원리는 Neuro-2a 세포주에 Na⁺/K⁺ ATPase 억제제인 Ouabain(O)과 Na⁺ 채널 활성화제인 Veratridine (V)을 처리하여 과도한 Na⁺ 유입으로 인한 세포사멸을 유도한 상태에서, Na⁺ 채널 억제제인 PSP를 처리하게 되면 Na⁺ 유입이 차단되어 세포가 생존하는 것을 측정하는 것이다. 본 연구에서는 PSP 검출을 위한 Neuro-2a assay를 국내 연구 환경에 맞게 다양한 매개변수를 개선하여 최적 시험법을 확립하고자 하였다. 고려한 매개변수들은 세포밀도, 배양 조건 및 PSP 처리 조건 등으로, 그 결과는 아래와 같다. 초기 세포밀도는 40,000 cells/well로, 세포 배양시간 및 처리시간은 각각 24시간으로 설정하였다. 또한 최적 O/V 농도는 500/50 μM로 설정하였다. 본 연구에서 PSP 중 Saxitoxin (STX)에 대해서 O/V 처리가 된 상태에서 S자형 용량-반응 그래프가 도출되는 8가지 농도(368~47,056 fg/μl)를 확인하였고, Neuro-2a assay의 실험실 간 변동성 비교를 통해, 실험의 적정성 확인을 위한 5가지 Quality Control Criteria와 실험 데이터의 신뢰가능 범위(Data Criteria) 6가지를 설정하였다. 확 립된 조건으로 Neuro-2a assay를 진행한 결과 반수영향농도(EC₅₀) 값은 약 1,800~3,500 fg/μl로 나타났다. 실험실 간 변동성 비교 결과, Quality Control Criteria 값 및 Data criteria 값의 변동계수(coefficients of variation (CVs))가 1.98~29.15% 범위로 산출되어 실험의 적정성 및 재현성이 확인되었다. 본 연구를 통해 우리나라에서 활용할 수 있는 PSP 검출용 Neuro-2a assay 시험법의 최적 조건 및 5가지 Quality control 기준을 제시하였고, PSP 중 대표적인 독소인 STX을 대상으로 Neuro-2a assay를 실시한 결과 유의한 EC₅₀ 값을 산출할 수 있었으며, 향후 국내 수산물을 대상으로 MBA를 대체할 수 있는 PSP 검출법으로 활용될 것으로 기대된다.