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The Comparison of Morphological and Constituents of the Leaves of Magnolia officinalis, M. biloba and M. obovata  

Park, Seo-Hee (College of Pharmacy, Chungnam National University)
Yun, Ui-Jung (College of Pharmacy, Chungnam National University)
Shin, Jong-Hun (Lab of Marine Natural Products and Structure Determination, Natural Products Research Institute, Seoul National University)
Kwon, Byoung-Mok (Molecular Cancer Research Center, Korea Research Institute of Bioscience & Biotechnology)
Bae, Ki-Hwan (College of Pharmacy, Chungnam National University)
Publication Information
Korean Journal of Pharmacognosy / v.37, no.4, 2006 , pp. 278-282 More about this Journal
Abstract
In this study, we analyzed and quantified the contents of magnolol (1), honokiol (2), and obovatol (3) in the leaves of Magnolia species by HPLC. HPLC methods were developed and validated by ICH guide lines comparing the linearity, intraday precision, inter-day precision. The optimal conditions were as follows; wavelength: 288 nm, column: Zorbax Eclipse XDBC18 $(2.1\times150\;mm,\;5{\mu}m)$, mobile phase: Acetonitril - 0.1 % $H_3PO_4$ (45:55, v/v) buffer, flow rate: 0.5 ml/min. The contents of magnolol, honokiol and obovatol were 1.08%, 2.37%, and 0.57% in the leaves of M. officinalis, 0.50%, 0.03%, and 1.54% in the leaves of M. biloba, 0.37%, 0.03%, and 0.76% in the leaves of M. obovata. The contents of effective ingredients in the leaves of M. officinalis and M biloba were higher than that of M. obovata. Especially, the content of obovatol in the leaves of M. biloba was remarkably high. This result showed that the shapes of leaves have a certain relationship with the content of obovatol.
Keywords
Magnolia officinalis; M. biloba; M. obovata; magnolol; honokiol; obovatol; HPLC;
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1 Bae, K., Jang B. S., Chung C. P. and Son S. H. (1993) The effects of honokiol and magnolol on the antimicrobial, bacterial collagenase activity, cytotoxicity and cytokine production. Kor. J. Academy of Periodontology. 23: 145-158
2 Ma, Peng, and Lin, Tao. (1993) Determination and comparison of magnolol and honokiol in Magnolia obovata by RP-HPLC. Huaxi Yaoxaue Zazhi. 8(2): 87-89
3 Momma, N., Sue, H., Hamaya, Y, Ueda, J. and Ohsawa, K. (1994) A simultaneous determination of honokiol and magnolol in kampo medicines containing Magnolia bark by ion-pair high-performance liquid chromatography. Yakugaku Zasshi. 114(10): 799-802   DOI
4 Hao, Q. H, Chen, G H., Feng, Y. Q. and Tian, Y L. (2005) Determination of the main efficient composition in the herb of Magnolia officinalis for eliminating oxygen radicals. Hebei Daxue Xuebao, Ziran Kexueban. 25(2): 164-166, 178
5 Zhang, Z. P., Hu, Z. D. and Yang, G L. (1997) Separation and determination of magnolol and honokiol in Magnolia officinalis bark by capillary zone electrophoresis. Mikrochimica Acta. 127(3-4): 253-258   DOI
6 강소신의학원 (1998) 중약대사전, 제 10권,6614. 상해과학출판사 출판, 도서출판 정담 발행, 서울
7 Ou, X., Zhang, L. and Xi, Y. (1984) Gas chromatographic assay of magnolol and honokiol in Magnolia officinalis and its extracts. Yaoxue Tongbao. 19(7): 421-423
8 Kwon, B. M., Kim, M. K, Lee, S. H., Kim, J. A., Lee, I. R., Kim, Y. K. and Bok, S. H. (1997) Acyl-CoA : Cholesterol Acyltransferase Inhibitors from Magnolia obovata. Planta Med. 63: 550-551   DOI   ScienceOn
9 Wang, X., Wang, Y, Geng, Y, Li, F. and Zheng, C. (2004) Isolation and purification of honokiol and magnolol from cortex Magnoliae officinalis by high-speed counter-current chromatography. J. Chromatography A. 1036: 171-175   DOI   ScienceOn
10 Bae, K. H., Kim, Y. H, Won, D. H, Lee, J. S. and Kang, J. S. (1997) Quality Evaluation on Magnoliae Cortex. Yakhak Hoeji. 41(4): 407-413
11 Si, J., Pan, X., Tong, Z. and Zeng, Y R. (2000) A study on bark quality of Magnolia officinalis Rehd. et Wils. China journal of Chinese materia medica. 25(8): 466-469
12 Shoji, Y., Takashi, N., Akihide K., Toshihiro N. and Itsuo, N. (1991) Isolation and Characterization of Phenolic Compounds from Magnoliae Cortex Produced in China. Chem. Pharm. Bull. 39: 2024-2036   DOI
13 Mikio, O., Hideaki, O. and Makoto, N. (1989) Makino's New Illustrated Flora of Japan, 117. The Hokuryukan Publishing Co., Tokyo, Japan
14 Zhou, Y. and Shen, J. (2001) Determination of honokiol and magnolol in concentrated Magnolia officinalis granules by HPLC. Guangdong Yaoxueyuan Xuebao. 17(3): 193-194
15 Kotani, A., Kojima, S., Hakamata, H., Jin, D. and Kusu, F. (2005) Determination of honokiol and magnolol by micro HPLC with electrochemical detection and its application to the distribution analysis in branches and leaves of Magnolia obovata. Chem. Pharm. Bull. 53(3): 319-322   DOI   ScienceOn
16 Hisashi, M., Tadashi, K., Mamiko, O., Toshio, M., Yasuko S. and Masayuki, Y. (2001) Effects of Constituents from the Bark of Magnolia obovata on Nitric Oxide Prodution in Lipopolysaccharide-Activated Macrophages. Chem. Pharm. Bull. 49: 716-720   DOI   ScienceOn
17 Zhou, G and Zhu, Z. (1985) Comparison tests of Shaanxi's Jiang Po (Magnolia) and Hou Po {Magnolia officinalis and M. officinalis var. biloba) by TLC and GLC. Zhongcaoyao. 16(3): 104-106
18 Bae, K. (1999) The Medicinal Plants of Korea, 114. Kyo-Hak Publishing Co., Seoul
19 Pang, L. Y, Chen, G H., Tian, Y. L. and Li, Y. Q. (2004) Determination of magnolol and honokiol in the herb of Magnolia officinalis by fluorescence spectrophotometry. Hebei Daxue Xuebao, Ziran Kexueban. 24(2): 159-162
20 Wang, G, Zhang, S., Zhang, Z. and Lei, Z. (2001) Determination of magnolol and honokiol in Magnolia officinalis and their supercritical fluid extract by capillary zone electrophoresis. Zhongguo Yaoxue Zazhi. 36(4): 265-267
21 Ito, K., Iida, T., Ihino, K, Masa, T, Masao, H. and Namba, T. (1982) Obovatol and Obovatal, Novel Biphenyl Ether Lignans from the Leaves of Magnolia obovata THUNB. Chem. Pharm. Bull. 30(9): 3347-3353   DOI   ScienceOn
22 Haraguchi, H., Ishikawa, H., Shirataki, N. and Fukuda, A. (1997) Antiperoxidative activity of neolignans from Magnolia obovata. Journal of Pharmacy and Pharmacology. 49(2): 209-212   DOI   ScienceOn
23 Si, J., Pan, X., Tong, Z. and Zeng, Y (1998) Study on the relationship between provenance, leaf type and quality in Magnolia officinalis. Journal of Chinese medicinal materials. 21(11): 541-543