• 한국균학회지
• /
• 제25권3호통권82호
• /
• pp.233-237
• /
• 1997

여름느타리버섯의 단포자에 UV를 처리하여 pab 요구성 영양요구주를 작성하였으며 이 균의 균사에서 원형질체를 분리한 후 Coprinus pab 1유전자를 함유하는 plasmid를 이용하여 prototrophy로 형질전환 하였다. 형질전환율은 ${\mu}g$의 DNA 당 5개의 형질전환주를 얻을 수 있었다. 형질전환주는 Southern 분석결과 염색체 DNA 속으로 integration된 것으로 확인되었으며 영양생장과 생식 생장시 모두 안정하게 유지되었다. 형질전환주와 화합성인 다른 영양요구주와 교배후 자실체의 포자를 분리하여 유전분석 결과 pab 생합성 유전자 보다는 그 유전자 주위에 integration이 일어난 것으로 추정되었다.

• 미생물학회지
• /
• 제29권1호
• /
• pp.16-24
• /
• 1991

The yeast gene THR1 encodes the homoserine kinase (EC 2.7.1.39: HKase) which catalyses the first step of the threonine specific arm at the end of the common pathway for methionine and threonine biosynthesis. A recombinant plasmid pMC3 (12.6 kilobase pairs, vector YCp50) has been cloned into E. coli HB101 from a yeast genomic library through its complementing activity of a thr1 mutation in a yeast recipient strain M39-1D. When subcloned into pMC32 (8.6kbp, vector YRp7) and pMC35 (8.3 kbp, vector YIp5), the HindIII fragment (2.7 kbp) of pMC3 insery was positive in the thrI complementing activity in both yeast and E. coli auxotrophic strains. The linearized pMC35 was introduced into the original recipient yeast strain and the mitotically stable chromosomal integrant was identified among the transformants. Through the tetrad analysis, the integration site of the pMC35 was localized to the region of THR1 structural gene at an expected genetic distance of approximately 11.1 cM from the ARG4 locus on the right arm of the yeast chromosome VIII. When episomically introduced into the auxotrophic cells and cultured in Thr omission liquid medium, the cloned gene overexpressed the HKase in the order of thirteen to fifteenfold, as compared with a wildtype. HKase levels are repressed by addition of threonine at the amount of 300 mg/l and 1, 190 mg/l for pMC32 and pMC3, respectively. Data from genetic analysis and HKase response thus support that the cloned HindIII yeast DNA fragment contains the yeast thr1 structural gene, along with necessary regulatory components for control of its proper expression.

• Journal of Microbiology and Biotechnology
• /
• 제24권11호
• /
• pp.1503-1509
• /
• 2014

With the purpose of facilitating the process of stable strain generation, a shuttle vector for integration of genes via a double recombination event into two ectopic sites on the Sulfolobus acidocaldarius chromosome was constructed. The novel chromosomal integration and expression vector pINEX contains a pyrE gene from S. solfataricus P2 ($pyrE_{sso}$) as an auxotrophic selection marker, a multiple cloning site with histidine tag, the internal sequences of malE and malG for homologous recombination, and the entire region of pGEM-T vector, except for the multiple cloning region, for propagation in E. coli. For stable expression of the target gene, an ${\alpha}$-glucosidase-producing strain of S. acidocaldarius was generated employing this vector. The malA gene (saci_1160) encoding an ${\alpha}$-glucosidase from S. acidocaldarius fused with the glutamate dehydrogenase ($gdhA_{saci}$) promoter and leader sequence was ligated to pINEX to generate pINEX_malA. Using the "pop-in" and "pop-out" method, the malA gene was inserted into the genome of MR31 and correct insertion was verified by colony PCR and sequencing. This strain was grown in YT medium without uracil and purified by His-tag affinity chromatography. The ${\alpha}$-glucosidase activity was confirmed by the hydrolysis of $pNP{\alpha}G$. The pINEX vector should be applicable in delineating gene functions in this organism.

• 한국지반공학회:학술대회논문집
• /
• 한국지반공학회 2009년도 세계 도시지반공학 심포지엄
• /
• pp.695-699
• /
• 2009

공정과 공사비는 건설 프로젝트의 관리에 있어서 매우 중요한 두 가지 요소로써, 그동안 이들을 통합 관리하려는 노력이 많이 이루어져 왔으나 국내의 국가계약법에 따른 내역체계의 한계점과 내역 및 WBS 표준화 작업의 미흡으로 인해 실제 시행에 많은 어려움을 겪고 있다. 본 연구에서는 최신관리기법인 BIM의 객체기반 3D 모델링 정보를 이용하여 비용과 일정정보를 연계하여 관리하는 방안을 제시하였다. 먼저 프로젝트 구성정보를 비용, 일정, 구조물의 부위별 구성요소로 나누고 각 구성정보에 의한 분류체계의 특징을 분석하였고, 국내의 내역중심 관리체계와 전체적인 관리 시스템 상의 한계를 감안하여 제약조건을 설정하고 작업분류체계(WBS)를 통한 일정정보를 중심으로 비용과 3D 모델링의 부위별 객체들을 연계하는 방안을 제시하였다. 제시된 방안의 검증을 위하여 대림산업에서 시공중인 청풍대교 현장에 대하여 사례연구를 실시하였으며, 이를 통하여 일정변화에 따른 시각화 및 비용예측 등의 기대효과를 확인하고 한계점 및 향후 추가 연구사항들을 가늠해볼 수 있었다.

• Journal of Positioning, Navigation, and Timing
• /
• 제11권4호
• /
• pp.327-332
• /
• 2022

The Korea Augmentation Satellite System (KASS) is the Satellite-Based Augmentation System (SBAS) under development in Korea. KASS navigation service support navigation Safety of Life (SoL) service. KASS signal provides corrections to Global Positioning System (GPS) data received from KASS Reference Stations (KRS) and is broadcast form Geostationary Earth Orbiting (GEO) satellites to KASS users and is used by GPS/SBAS user equipment to improve the accuracy, availability, continuity and integrity of the navigation solution. Seven KRS's collect the satellite data and send them to the KASS Processing Stations (KPS) for the generation of the corrections and the monitoring the integrity. For performing its computation the KPS needs to know accurate and reliable KRS antennas coordinates. These coordinates are provided as configuration parameters to the KPS. This means that the reference frame in which the KPS work is the one represented by the set of coordinates provided as input. Therefore, the activity to maintain the accuracy of the KRS antenna coordinates is necessary, knowing that coordinates can evolve due to earth plates movements or earthquakes. In this paper, we analyzed the geodetic survey results for KRS antenna coordinates from Site Acceptance Test (SAT) #1 in December 2020 to August 2022. In the future, it is expected that these activities and planning for KRS coordinates maintenance will be produced and provided to KASS system operators for KPS configuration updates during the KASS lifetime of 15 years. Through these maintenance activities, it is expected that monitoring and analysis of unpredictable events such as earthquakes and seism will be possible in the future.

• 패션비즈니스
• /
• 제13권6호
• /
• pp.20-30
• /
• 2009

The purpose of this study is to develop the effective customized elderly fashion marketing process based on the web site, where older customer will be able to choose various fabrics and to try them out. This aims to establish new prototype of internet shopping mall for customized elderly fashion clothing. In this study, new method of product presentation on the online shopping mall is proposed to offer product information through 3D virtual reality. With the online shopping mall(SATC Mall) as a showcase, we presented virtual mapping system so that it enable the customers to select the fabrics and to see exactly how chosen fabric will look when applied to image of clothing. As an initial test of the application of simulation to measure 3D visualization of product, mapping software Vision Easy Map Pro Version 6.0(NedGraphics) Vision Easy Map Viewer Version 5.0(NedGraphics) were chosen and applied. By using this mapping system, the fabric change of the apparel product could be made on the internet shopping web site. However, this approach has been successful applied for presenting and customizing garment products. Future research will focus on the integration of mapping coordination into SATC Mall.

• Structural Engineering and Mechanics
• /
• 제30권3호
• /
• pp.331-350
• /
• 2008

A framework for multi-platform analytical and multi-component hybrid (testing-analysis) simulations is described in this paper and illustrated with several application examples. The framework allows the integration of various analytical platforms and geographically distributed experimental facilities into a comprehensive pseudo-dynamic hybrid simulation. The object-oriented architecture of the framework enables easy inclusion of new analysis platforms or experimental models, and the addition of a multitude of auxiliary components, such as data acquisition and camera control. Four application examples are given, namely; (i) multi-platform analysis of a bridge with soil and structural models, (ii) multiplatform, multi-resolution analysis of a high-rise building, (iii) three-site small scale frame hybrid simulation, and (iv) three-site large scale bridge hybrid simulation. These simulations serve as illustrative examples of collaborative research among geographically distributed researchers employing different analysis platforms and testing equipment. The versatility of the framework, ease of including additional modules and the wide application potential demonstrated in the paper provide a rich research environment for structural and geotechnical engineering.

• 환경영향평가
• /
• 제4권1호
• /
• pp.1-8
• /
• 1995

Environmental Impact Assessment(EIA) in Korea has developed as a tool for environmental conservation and decision making since Environmental Impact Statement(EIS) preparation in 1981. For effective implementation of it, we do system development and method enhancement continuously. The recently introduced GIS (Geographic Information System) can integrate geographic and attribute data, which will be applied to scoping, assessment, and alternative assessment, etc. in EIA. This study has three major components. First, it explains building of EIA factors of natural environment, living environment, and socio-economic environment of the study area defined in EIS preparation regulation. Second, the study presents applications of assessment method concerning suitability analysis of landfill site using GIS. Finally, it shows integration of GIS and odor impact assessment. Based on these analysis, the study makes some recommendations and conclusions.

• 한국실내디자인학회논문집
• /
• 제26권5호
• /
• pp.16-24
• /
• 2017

This study examines Safdie's design philosophy: place, purpose, and process and design strategies found in museum buildings. Safdie, influenced by personal background as immigrant, education of McGil University in Canada, apprenticeship from Louis Kahn, is one of the most recognized architects who designed renowned public and cultural buildings after Habitat 67 in Montreal, Canada. In order to reveal design strategies his eight museum buildings has been selected and analyzed in terms of interpretation of context, program presented in floor plan and section design, and the process of integrity found in structure and material. The results of this paper can be summarized as follows: 1) Reflection of existing context and regeneration of the locality has been in a creative way to reinforce the sense of place. 2) The layout of major and gallery space is determined according to the Safdie's interpretation of the site context and this can be categorized into three types. 3) Integration of the innovative structural frame and material has been experimented by using tartan grid and toroid form in the major and gallery spaces. This study is intended to reevaluate the significance of Safdie's design approach to building uniqueness of the site, program and tectonic.

• 패션비즈니스
• /
• 제17권3호
• /
• pp.1-14
• /
• 2013

This study is about the evaluation of the color arrangements of working clothes that are harmonized with working environment and assist in the protection of workers in industrial sites. This study attempted color arrangement design with the intention of giving order to the colors of working environment and working clothes so as to help improve the safety of work and the management aspects including human resource management, efficiency improvement, and the practice of business ethics. The study method consisted of literature research, empirical research, and evaluation research. The literature research performed theoretical examination about colors, industrial safety, and visibility. Three actual outdoor work sites in the machine industry were chosen and pictures were taken at 20-30 m from workers. The background colors of the work environment of each work site and the colors of the working clothes that were actually worn were analyzed through color values measured with a color-difference meter(CR-400). As a result, it was found that between value and chroma which affect visibility, color arrangement based on differences in chroma could be a greater influence than color arrangement based on differences in value. The results of this study could assist the integration of color schemes among working environment colors, people and working clothes by suggesting color arrangements with improved visibility that are applicable to the working clothes of domestic machine manufacturers that are playing a pivotal role in domestic industries and by presenting appropriate guidelines.